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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Enzymology
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Proprotein Convertases (Paperback, Softcover reprint of the original 1st ed. 2011)
Loot Price: R4,067
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Proprotein Convertases (Paperback, Softcover reprint of the original 1st ed. 2011)
Series: Methods in Molecular Biology, 768
Expected to ship within 10 - 15 working days
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Today, activation endoproteolysis of secretory proteins is
recognized as a fundamental biological mechanism of spatial and
temporal regulation of protein activity as well as of
diversification of protein functions. In Proprotein Convertases,
experts in the field examine detailed methods involving proprotein
convertases, the enzymes mediating this endoproteolysis, which
reside within or cycle between the various compartments of the
secretory pathway. Providing a timely assessment of impact of
activation/inactivation endoproteolysis in the secretory pathway,
the volume offers a broader perspective on the biochemistry of the
PCSKs (proprotein convertases, subtilisin/kexin-type) by exploring
structural and functional analogies with bacterial subtilisin and
on the enzymology of endoproteolysis itself by describing the
involvement in the process of non-PCSK-type such as cathepsin L.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their specific
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Meticulous and
up-to-date, Proprotein Convertases represents an instructive and
useful reference book for all scientists interested in
endoproteolytic activation and/or inactivation of secretory
proproteins through limited proteolysis, for experts in the field
and newcomers to it as well.
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