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Laboratory Guide to Biochemistry, Enzymology and Protein Physical Chemistry - A Study of Aspartate Transcarbamylase (Paperback)
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Laboratory Guide to Biochemistry, Enzymology and Protein Physical Chemistry - A Study of Aspartate Transcarbamylase (Paperback)
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Total price: R2,565
Discovery Miles: 25 650
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1 Aspartate Transcarbamylase.- 2 Molecular Genetics: Regulation of
Aspartate Transcarbamylase Biosynthesis.- 3 Purification of
Aspartate Transcarbamylase and Its Subunits.- 4 Structural and
Physicochemical Study of Aspartate Transcarbamylase.- 5 Enzymatic
Catalysis and Regulation.- 6 Complementary Experiments.- 1.
Equations and Units.- 2. Molar Mass and Molecular Mass.- 3. Units
of Catalytic Activity.- 4. Units of Radioactivity.- 5. Units of
Quantity.- 7. Calculation of Acceleration.- 8. Bacterial Strains.-
9. Solutions and Reagents.- 9.1. 0.8-M Tris-Acetate Buffer, pH 8.-
9.2. 0.2-M Acetic Acid.- 9.6. Elution Buffer for Chromatography: 10
x Stock Solution.- 9.7. 100-mM Aspartate, pH 8.- 9.8. 10-mM
Carbamylaspartate, pH 8.- 9.9. 1-M Phosphate Buffer, pH 7.2.- 9.10.
Buffer for Dilution of E.- 9.11. Buffer for Dilution of C.- 9.12.
Buffer for Dilution of R and Recombination.- 9.13. 200-mM
Cacodylate Buffer, pH 6, 7, and 7.5.- 9.14. 200-mM Tris-Acetate
Buffer, pH 8 and 9.- 9.15. 200-mM Glycine Buffer, pH 10.- 9.16.
400-mM Succinate, pH 7.- 9.17. Reaction Medium: 20-mM CAP-200-mM
Tris-Acetate, pH 8.- 10. Preparation of Standard Protein Mixtures
for Column Calibration.- Answers To Questions.- References.
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