Gene Transfer and Expression - A Laboratory Manual (Paperback, New edition)

This book presents a descriptive, comprehensive catalogue of viral and cellular cis-acting elements and the latest expression vectors for obtaining optimum expression of foreign genes introduced into mammalian cells. Included is a selection of optimized, state-of-the-art protocols for transferring genes into mammalian cells and subsequently assaying for transfer and expression of the genes of interest. This unique volume describes in detail essential techniques for propagating cells and cell lines, DNA transfer techniques, drug selection and gene amplification techniques, expression cloning, subtractive hybridization and retrovirus-mediated gene transfer techniques. Also featured are highly sensitive assays for gene transfer and expression, including detection of DNA and RNA by both isotopic and non-isotopic detection methods as well as the most sensitive non-isotopic Western techniques for the detection of proteins.

General

Imprint:	W. H. Freeman
Country of origin:	United States
Release date:	September 1993
Authors:	Michael Kriegler
Dimensions:	280 x 220 x 16mm (L x W x T)
Format:	Paperback
Pages:	242
Edition:	New edition
ISBN-13:	978-0-7167-7004-6
Categories:	Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > General Books > Science & Mathematics > Biology, life sciences > Zoology & animal sciences > Vertebrates > Mammals > General Promotions
LSN:	0-7167-7004-0
Barcode:	9780716770046

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