Min Li and a panel of hands-on experimentalists detail
state-of-the-art molecular techniques for studying NMDA
ligand-gated ion channels and developing assays for nontherapeutic
lead selection. The topics range from cDNA cloning to in vitro and
in vivo investigation of the channel complex in the mammalian
brain. Additional topics include the biochemical analysis of the
channel protein and the construction of various heterologous
systems for both basic research and high throughput screens (HTS)
for pharmaceutical chemicals. Although the focus is on NMDA
receptors, the methods are applicable to other ligand-gated ion
channels and with some modification may be extended to related
membrane signaling receptors. NMDA Receptor Protocols offers
today's scientists powerful methods for basic research on NMDA
receptor structure and function, as well as enormous opportunities
for clinical investigation toward the development of novel
bioactive compounds.
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