RNA interference has become a key method in the suppression of
gene expression and the development of therapeutic agents, yet
there is still the problem of delivery, stability, and the danger
of off-target effects such as the silencing of unwanted genes and
activation of innate immunity. In siRNA and miRNA Gene Silencing:
From Bench to Bedside, expert researchers explore the most recent
advances in siRNA design, expression, delivery, in vivo imaging,
and methods to minimize siRNA s unwanted effects and promote
successful use in patients. As part of the highly successful
Methods in Molecular Biology series, the chapters focus on their
respective subjects with easy-to-use, up-to-date information,
including several step-by-step laboratory protocols on topics such
as new delivery formulations and strategies with promising
applications in vitro and in vivo, validated therapeutic target
genes, and components of miRNA function, biogenesis, and
interference with virus infection.
Comprehensive and cutting-edge, siRNA and miRNA Gene Silencing:
From Bench to Bedside offers an excellent collection of chapters to
aid all those with an interest in RNAi, gene regulation, and new
therapies."
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