The production of recombinant biopharmaceuticals with antiviral and
antiproliferative properties is one of the major aims of advanced
genetic engineering. Interferon alpha 2b belongs to the class of
recombinant therapeutics which is equally effective against viral
infections and carcinomas. Recombinant human IFN 2b is widely used
FDA approved protein drug with global sale level is expected to
reach up to 5.8 billion US dollars by 2010 due to its increased
demand as a result of high prevalence of hepatitis infections and
carcinomas in developed as well as developing countries including
Pakistan. In the current research a process was developed for the
production of this recombinant interferon alpha 2b drug by adopting
advanced molecular and biochemical strategies. Intragene
modifications at specific amino acids were also made to produce
antiviral gene sequences with improved antiviral properties. Human
interferon alpha 2b gene analysis was also established in blood
cancers (AML and ALL) in order to find out mutations of clinical
significance in theses cancers.
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