Hepatocytes account for approximately 80% of the liver mass and
play a significant role in various aspects of liver
physiopathology, exhibiting unrivaled complexity and diversity of
functions. In Hepatocytes: Methods and Protocols, expert
researchers provide the reader with methods, technical protocols,
and review chapters focusing on selected areas of hepatocyte
biology including isolation, culture, differentiation and stem
cells, and hepatocyte use in clinical, basic, and applied research.
With a specific emphasis on human hepatocytes, the volume presents
chapters covering subjects including hepatocyte culture models,
cryopreservation methods, differentiation assessment, liver
ontogenesis, production of hepatocytes from stem cells,
drug/xenobiotic metabolism, toxicity and transport, bile acid and
blood coagulation factor production, infection by HBV and HCV,
humanized animals, biortificial liver devices, hepatocyte
transplantation. As a volume in the highly successful Methods in
Molecular Biology (TM) series, protocol chapters include brief
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and notes on troubleshooting and avoiding
known pitfalls. Comprehensive and cutting-edge, Hepatocytes:
Methods and Protocols will be useful to all those who are currently
using or planning to use human, or animal, hepatocytes to
investigate any aspect of liver physiopathology or who are
interested in liver development or liver stem cells and liver
biotherapy.
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