Human Pluripotent Stem Cells - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2011)

Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments. Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better in vitro human disease models; 2) the discovery that a Rho-associated protein Kinase (ROCK) inhibitor allows for efficient single cell passaging and cryopreservation, increasing the efficiency and reliability of hPSC culture; and 3) defined, animal-component-free media, which lay the groundwork for simplified scale-up for therapeutic applications, differentiation protocols, and toxicology screens. The aforementioned technologies can be found in Human Pluripotent Stem Cells: Methods and Protocols, a compilation of 33 detailed protocols in six categories of PSC research that cover laboratory essentials and the derivation of new PSC lines, including induced PSC lines, as well as their growth, maintenance, characterization, genetic manipulation, and differentiation. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Human Pluripotent Stem Cells: Methods and Protocols serves as an ideal guide to scientists conducting their own pluripotent cell research programs and makes great strides towards furthering human knowledge and, ultimately, improving the human condition.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 767
Release date:	August 2016
First published:	2011
Editors:	Philip H Schwartz • Robin L. Wesselschmidt
Dimensions:	254 x 178mm (L x W)
Format:	Paperback
Pages:	468
Edition:	Softcover reprint of the original 1st ed. 2011
ISBN-13:	978-1-4939-5802-3
Categories:	Books > Science & Mathematics > Biology, life sciences > Cellular biology > General Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > General Promotions
LSN:	1-4939-5802-X
Barcode:	9781493958023

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