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Books > Science & Mathematics > Biology, life sciences > Molecular biology
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Proteoglycan Protocols (Hardcover, 2001 ed.)
Loot Price: R4,864
Discovery Miles 48 640
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Proteoglycan Protocols (Hardcover, 2001 ed.)
Series: Methods in Molecular Biology, 171
Expected to ship within 10 - 15 working days
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Proteoglycans are some of the most elaborate macromolecules of
mammalian and lower organisms. The covalent attachment of at least
five types of glycosami- glycan side chains to more than forty
individual protein cores makes these molecules quite complex and
endows them with a multitude of biological functions. Proteoglycan
Protocols offers a comprehensive and up-to-date collection of
prepa- tive and analytical methods for the in-depth analysis of
proteoglycans. Featuring st- by-step detailed protocols, this book
will enable both novice and experienced researchers to isolate
intact proteoglycans from tissues and cultured cells, to establish
the composition of their carbohydrate moieties, to generate
strategies for prokaryotic and eukaryotic expression, to utilize
methods for the suppression of specific proteoglycan gene
expression and for the detection of mutant cells and degradation
products, and to study specific interactions between proteoglycans
and extracellular matrix proteins as well as growth factors and
their receptors. The readers will find concise, yet comprehensive
techniques carefully drafted by leading experts in the field. Each
chapter commences with a general Introduction, followed by a
detailed Materials section, and an easy-to-follow Methods section.
An asset of each chapter is the extensive notation that includes
troubleshooting tips and practical considerations that are often
lacking in formal methodology papers. The reader will find this
section most valuable because it is clearly provided by experienced
scientists who have first-hand knowledge of the techniques they
outline. In addition, most of the chapters are well illustrated
with examples of typical data generated with each method.
General
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