Fluorescence-based sensing is a significant technique used in
prominent fields such as fluorescence-activated cell sorting, DNA
sequencing, high-throughput screening, and clinical diagnostics.
Fluorescence Sensors and Biosensors emphasizes the most recent
developments and emerging technologies with the broadest impacts.
The text begins with the development of aptamers
(oligoribonucleotides) and biorecognition techniques based on
periplasmic binding proteins. The following chapters review the
molecular beacon approach for DNA recognition, describe resonance
energy transfer (FRET) in sensing, and present the use of carbonic
anhydrase recognition platform for metal ion determination and
imaging. The book explores the advantages of fluorophores,
fluorescent labels, sensor and assay construction, metal-enhanced
fluorescence, phosphorescent labels, and lab-on-a-chip
applications. It also describes new anion-selective fluorescent
probes used as analytes in clinical determinations.
The final chapters highlight the application of fluorescence
sensing technology to several practical problems, such as the
development of planar waveguide biosensors for clinical diagnostics
and the adaptation of fluorescence-based sensing approaches for
biochemical production by fermentation. The book also discusses the
measurement of analytes, such as free zinc ions, at ultratrace
levels in biological specimens. Written by internationally renowned
authors in their fields, Fluorescence Sensors and Biosensors
provides an up-to-date account of fluorescence-based sensors
focused on practical applications in biotechnology, analytical
chemistry, and biomedicine.
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