Over the course of the past decade, there have been remarkable
advances in the study of human pathogenic fungi. These developments
have taken place throughout a wide range of disciplines, and have
come as the result of newly available genome sequences of pathogens
such as candida albicans and other model fungi. In Candida
Albicans: Methods and Protocols, expert researchers explore these
exciting new insights, focusing on the study of medically important
fungi and Candida spp in particular. Chapters examine critical
aspects of molecular methods, providing information on reporter
gene assays, transformation, gene expression in vivo, and methods
for large-scale gene disruption. At the same time, the work
includes in-depth descriptions of disease models of candidiasis,
facts about strain identification, and guidelines on the
preparation of samples for proteomic investigations and tandem
affinity purification. Composed in the highly successful "Methods
in Molecular Biology " series format, each chapter contains a brief
introduction, step-by-step methods, a list of necessary materials,
and a Notes section which shares tips on troubleshooting and
avoiding known pitfalls.
Authoritative and cutting edge, Candida Albicans: Methods and
Protocols is an invaluable source of methods for investigators in
the exhilarating fields of medical and molecular mycology."
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