Numerous pharmaceutical substances and their metabolites have been
identified in the aquatic environment. Due to their unique
environmental fate and lack of appropriate biomonitoring
techniques, the potential risk of these compounds to public and
environmental health has largely been undetermined. Recent interest
in quantifying pharmaceuticals in waste effluents and aquatic
environments has identified the need to develop bioanalytical
assays that will accurately reflect presence of these compounds.
The goal of this research was to investigate the use of DNA
microarrays as a bioanalytical tool for analysis of pharmaceutical
contamination in re-use waters. Results from this study suggest
that prototypic pharmaceutical contaminants target molecular events
associated with pharmaceutical-induced gene induction and
repression in exposed organisms. Gene expression profiles are
subsequently evaluated as a means to discern pharmaceutical
exposure. When fully developed, this bioanalytical approach will
provide an efficient and robust method for screening pharmaceutical
contaminants in reuse and other water matrices.
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