|
Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
|
Buy Now
Advances in GAPDH Protein Analysis: A Functional and Biochemical Approach (Hardcover, 1st ed. 2017)
Loot Price: R2,653
Discovery Miles 26 530
|
|
|
Advances in GAPDH Protein Analysis: A Functional and Biochemical Approach (Hardcover, 1st ed. 2017)
Expected to ship within 18 - 22 working days
|
This book presents modern and classic analytical approaches that
are crucial for the biochemical and functional characterization of
the archetypal protein, glyceraldehyde-3-phosphate dehydrogenase
(GAPDH). The distinguishing feature of the book is that it covers,
in addition to other methods, some of the uncommon but valuable
techniques as well. For example, in-gel visualization of enzyme
activity, immunoblotting protocols for native (non-denatured)
proteins, and proteins resolved by pH-gradient
[IEF-isoelectrofocusing], etc. These expedient methods are relevant
and vital for the verification of biochemical properties of GAPDH,
or similar protein of interest. This work outlines detailed
protocols that are essential to investigate classical (cellular)
and recently reported extracellular (secretory) isoforms of GAPDH.
Precisely, the book covers techniques pertinent to enzymatic and
non-enzymatic analysis of GAPDH that include, but not limited to,
electrophoretic mobility shift assay (EMSA), two-dimensional
(2D)-immunoblotting, immunofluorescence/confocal microscopy, mass
spectrometry, ion-exchange and affinity chromatography. Readers
will discover the importance of the experimental methods described
in the book as they relate to the evaluation of the role and
significance of GAPDH. Furthermore, majority of the methods
described in the book have also been validated in the author's
laboratory, besides other research groups worldwide, underlining
the repeatability and reproducibility of the protocols. Each method
begins with an abstract and a brief background emphasizing its
application and relevance. This will enable the readers to
determine the choice of experimental design according to their
research objectives. The book explains the methods systematically
with ample illustrations to facilitate quick and easy comprehension
of the practical knowledge. Although the book is focused on GAPDH,
many of the protocols may be adopted to other proteins or enzymes
with minimal modifications. Noteworthy, it is unequivocally
established that GAPDH is a multifunctional protein involved in
several cellular processes of health & disease conditions.
Hence, this book will be a valuable practical guide for young
researchers, scientists and clinician-scientists.
General
Is the information for this product incomplete, wrong or inappropriate?
Let us know about it.
Does this product have an incorrect or missing image?
Send us a new image.
Is this product missing categories?
Add more categories.
Review This Product
No reviews yet - be the first to create one!
|
You might also like..
|
Welcome to Loot.co.za!
Sign in / Register |Wishlists & Gift Vouchers |Help | Advanced search
