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Books > Science & Mathematics > Science: general issues > Scientific equipment & techniques, laboratory equipment > Microscopy
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Confocal Microscopy - Methods and Protocols (Hardcover, 2nd ed. 2014)
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Confocal Microscopy - Methods and Protocols (Hardcover, 2nd ed. 2014)
Series: Methods in Molecular Biology, 1075
Expected to ship within 12 - 17 working days
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Confocal Microscopy: Methods and Protocols, Second Edition takes
the researcher from the bench top through the imaging process, to
the page. Protocols for the preparation of tissues from many model
organisms including worms, flies and mice have been included as
well as chapters on confocal imaging of living cells, three
dimensional analysis, and the measurement and presentation of
confocal images for publication. Emphasis has been placed on the
laser scanning confocal microscope since this is still the
instrument used for most routine applications. The current
generation of modern confocal instruments produces optical sections
of cells and tissues that are free of out-of-focus fluorescence
with reduced chances of artifacts from the techniques of specimen
preparation. This allows the imaging of living specimens and
measurements of physiological events within cells. Confocal
microscopy has become essential in many fields of contemporary
biomedical research where a light microscope is required for
imaging fluorescently labeled cells and tissues, especially cell
biology, developmental biology, neurobiology, and pathology.
Written in the successful Methods in Molecular Biology series
format, chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step,
readily reproducible protocols, and notes on troubleshooting and
avoiding known pitfalls. Authoritative and easily accessible,
Confocal Microscopy: Methods and Protocols, Second Edition is aimed
primarily, but not exclusively, at the novice user with pointers to
more advanced techniques.
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