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Books > Science & Mathematics > Biology, life sciences > Microbiology (non-medical)
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Hydrocarbon and Lipid Microbiology Protocols - Biochemical Methods (Paperback, Softcover reprint of the original 1st ed. 2016)
Loot Price: R2,776
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Hydrocarbon and Lipid Microbiology Protocols - Biochemical Methods (Paperback, Softcover reprint of the original 1st ed. 2016)
Series: Springer Protocols Handbooks
Expected to ship within 10 - 15 working days
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This Volume provides protocols for the biochemical analysis of
hydrocarbon- and lipid-relevant products, cell components and
activities of microbes that interact with hydrophobic compounds.
They include methods for the extraction, purification and
characterisation of surface tension-reducing bioemulsifiers and
biosurfactants that increase the surface area and hence
bioavailability of hydrophobic substrates. Protocols for the
isolation and biochemical analysis of lipids and
polyhydroxyalkanoates, food storage products made during nutrient
abundance that represent important biotechnological products, are
presented. The extraction of membrane lipid rafts, sub-organelles
that fulfil important functional roles for the cell membrane, and
the isolation and characterisation of membrane phospholipid
biomarkers, are also described. The purification and
characterisation of integral membrane hydrocarbon-oxidising enzymes
are addressed. Lastly, two generic methods for the genetic analysis
of catabolic pathways and analysis of ligand binding are presented.
Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of
thousands of structurally different hydrocarbons, hydrocarbon
derivatives and lipids, and a wide array of these molecules are
required for cells to function. The global hydrocarbon cycle, which
is largely driven by microorganisms, has a major impact on our
environment and climate. Microbes are responsible for cleaning up
the environmental pollution caused by the exploitation of
hydrocarbon reservoirs and will also be pivotal in reducing our
reliance on fossil fuels by providing biofuels, plastics and
industrial chemicals. Gaining an understanding of the relevant
functions of the wide range of microbes that produce, consume and
modify hydrocarbons and related compounds will be key to responding
to these challenges. This comprehensive collection of current and
emerging protocols will facilitate acquisition of this
understanding and exploitation of useful activities of such
microbes.
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