Classical natural product chemistry is transitioning to modern day
metabolomics as a result of the advent of comprehensive analytical
platforms and sensitive analytical instrumentation. Therefore, it
is worthwhile to summarize recent developments with current
analytical platforms and highlight how metabolomics is being
integrated into this classical field to dereplicate and profile
natural product extracts. Metabolomics Tools for Natural Product
Discoveries: Methods and Protocols aims to unite diverse and
recently developed methodologies and protocols in order to identify
bioactive secondary metabolites for the purpose of drug discovery.
Some topics covered in this volume include applications for the
extraction of selected natural products from less common sources
such as bryophytes and hard corals, various biological assays,
comprehensive applications and strategies for GC-MS, LC-MS, and
NMR, as well as protocols and strategies for the structure
elucidation of isolated natural products. Written in the successful
Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
protocols, and notes on troubleshooting and avoiding known
pitfalls. Authoritative and easily accessible Metabolomics Tools
for Natural Product Discoveries: Methods and Protocols seeks to
serve both professionals and research students with its well-honed
methodologies for natural product isolation, biomarker discovery,
dereplication, biological assays, and comprehensive metabolomic
platforms available for high-throughput analyses.
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