Basic Confocal Microscopy, Second Edition builds on the successful
first edition by keeping the same format and reflecting relevant
changes and recent developments in this still-burgeoning field.
This format is based on the Confocal Microscopy Workshop that has
been taught by several of the authors for nearly 20 years and
remains a popular workshop for gaining basic skills in confocal
microscopy. While much of the information concerning fluorescence
and confocal microscopy that made the first edition a success has
not changed in the six years since the book was first published,
confocal imaging is an evolving field and recent advances in
detector technology, operating software, tissue preparation and
clearing, image analysis, and more have been updated to reflect
this. Several of these advances are now considered routine in many
laboratories, and others such as super resolution techniques built
on confocal technology are becoming widely available.
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