Recombinant Protein Expression: Prokaryotic hosts and cell-free systems, Volume 659 (Hardcover)

Recombinant Protein Expression, Part A, Volume 659 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more. Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr (R) 250, and Expression of recombinant antibodies in Leishmania tarentolae.

General

Imprint:	Academic Press Inc
Country of origin:	United Kingdom
Series:	Methods in Enzymology
Release date:	October 2021
First published:	2021
Volume editors:	William B. O'Dell • Zvi Kelman
Dimensions:	229 x 152mm (L x W)
Format:	Hardcover
Pages:	460
ISBN-13:	978-0-323-90146-8
Categories:	Books > Science & Mathematics > Biology, life sciences > Molecular biology Books > Science & Mathematics > Biology, life sciences > Biochemistry > General Promotions
LSN:	0-323-90146-8
Barcode:	9780323901468

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