How the Human Genome Works covers the essential principles of genetics in a readable, accessible format using real-life examples of the way genes affect human behavior, health and illness, development, and evolution. Simple two-color graphics and color highlighting illustrate important concepts throughout, and the book also entails a glossary of molecular genetic terms. The text is intended for all readers who need an introduction to, or refresher on human genetics, and includes science, health, medicine, and nursing students, as well as professionals in related fields.

DNA transfer to cultured cells
Edited by Katya Ravid and R. Ian Freshney
Rapid advances in DNA transfer technology have transformed many disciplines, ranging from molecular genetics to biotechnology. Scientists now have the means to introduce copies of genes into different cell types, then detect the expression of these genes in the cell. It is now possible to regulate cell growth that may lead to cancer, develop new biopharmaceuticals, and apply knowledge about the role of genes in cell processes to basic research in molecular genetics.
DNA Transfer to Cultured Cells is the first quick reference to all of the established techniques for the transfer of genetic material to cells in vitro. Featuring contributions by leading researchers in the field, this detailed guide walks the reader through a variety of DNA transfer methods, describes their application to specific cell types, and integrates aspects of molecular biology with tissue culture. Offering overviews and detailed protocols for the techniques under discussion in each of its sections, this book covers an exceptionally broad array of topics, including:
* Viral infection
* Electroporation
* Phosphate precipitation
* DEAE Dextran
* Liposomes
* Yeast artificial chromosomes (YACs)
* Whole chromosome transfer
* Enhanced expression.
Special sections at the end of each chapter list suppliers for necessary reagents and materials. This easy-to-use, self-contained guide addresses key developments of recent years as well as emerging trends in DNA transfer. For practical applications in cell biology, genetics, heredity, biotechnology, or evolution, DNA Transfer to Cultured Cells is a uniqueand unparalleled resource.

Fun and educational, these unique playing cards are beautifully illustrated with detailed cells and cellular structures - perfect for science lovers and cell biologists of all ages! Card faces features favorite illustrations from the textbook Cell Biology by Thomas D. Pollard et al. - from the clathrin triskelion and its three-fold symmetry on the 3 to mitotic chromosome structure on the Queen! Standard 52-card deck with illustrations of echinospherium, seipin ring, muscle thin filament with troponin-tropomyosin, and more. Jokers feature illustrations of cdk2-cyclin A (red joker) and endoplasmic reticulum (black joker). Fourteen different card faces in all featuring the well-loved cellular illustrations of Graham Johnson!

This collection of articles deals with the benefits of different types of stem cells sources, use, manipulation, and aspects for the treatment of chronic diseases. Topics include the role of calcium channel pathway/s in the regulation of neural stem cell differentiation; the evolutionary roles of the totipotent, pluripotent, or even multipotent stem cells; the derivations of multipotent MSCs; and potential canine-derived stem cell therapies for dogs. The scope of the book also provokes further studies into other topics, such as MSC differentiation into hepatocytes and the involvement of these cells with microRNA-133 in type 1 diabetes; the role of Vitronectin in the differentiation into endoderm; the extent to which the stemness of dental pulp stem cells might be useful; PBMCs as a source for pluripotent stem cells; yoga in possible synergy with the finding that bone marrow stromal cells provide relief from a laboratory reagent used as drug-mediated pain in spinal cord injury.

This book gives an up-to-date overview of the biomedical aspects of autophagy research, a large interdisciplinary field emerging on intersection of (i) cell, developmental, and cancer biology, (ii) tissue remodelling and plasticity, ageing and longevity; (iii) immunology, microbiology, toxicology, and pathobiology; (iv) regenerative and adaptive biomedicine; and (v) pharmacology.

Myosin: Biosynthesis, Classes and Function opens with a discussion on class I myosins, the most varied members of the myosin superfamily and a remarkable group of molecular motor proteins that move actin filaments and produce force. Class I myosin molecules have various physiological roles including maintenance of normal intestinal brush border structure, glucose homeostasis, glomerular filtration, immune function, and tumor promotion and suppression, and new studies are revealing that mutations may lead to diseases including cancer and kidney disease. Thus, the authors review the structure and function of the eight myosin-I isoforms (Myo1a-Myo1h) that are expressed in mammals. Next, the book discusses muscle contractile function and its association with the activity of the protein complex actomyosin, in which myosin exhibits enzyme activity, namely the ability to hydrolyze ATP. The demonstrated ability of calix[4]arenes C-97, C-99, C-90 and thiacalix[4]arenes C-798 and C-800 can be used for further research aimed at the use of these compounds as novel pharmacological agents able to efficiently restore normal contractile function of myometrium by inhibition or activation of this function, or the eliminating negative effects of heavy metal cations. Following this, the authors present the results of their experiments on studying the effects of different isotopes of magnesium and zinc on the enzymatic activity of myosin, namely the catalytic subfragment-1 of myosin, isolated from myometrium muscle. It has been revealed that the rate of the enzymatic ATP hydrolysis is 22.5 times higher in the reaction media enriched with the magnetic isotope, 25Mg, as compared to the activity of the same enzyme in the reaction media enriched with the nonmagnetic isotopes, 24Mg or 26Mg or MgCl2 of natural isotope abundance. Continuing, precipitation/extraction methods and MALDI TOF/TOF mass spectrometry were used in order to and identify, for the first time, a protein with the molecular mass of 48 kDa as a fragment of human unconventional myosin 1c isoform b in a blood serum of multiple sclerosis patients. Western-blot analysis using commercial monospecific anti-human Myo1c antibodies has shown that the molecular mass of this protein obtained from a blood serum of different human sources varied in between 46-48 kDa. Thus, the authors name the 46-48 kDa proteins revealed in a blood serum as a short form of the human unconventional myosin 1c (sMyo 1C).

"Liposomes En Biologie Cellulaire Et Pharmacologie".

Each volume in this richly illustrated series, published in association with the Papanicolaou Society of Cytopathology, provides an organ-based approach to the cytologic and histologic diagnosis of small tissue samples. Benign, pre-malignant and malignant entities are presented in a well-organized and standardized format, with high-resolution color photomicrographs, tables, and lists of key specific morphologic criteria. Example vignettes allow the reader to assimilate the diagnostic principles in a case-based format. This volume provides comprehensive coverage of both surgical pathology and cytopathology of focal liver lesions. Extensively illustrated throughout, it contains key cytologic and histologic features, practical points, radiologic and morphologic pictures, flow charts, and tabulated summaries for easy comprehensive overview and quick reference and provides a pragmatic algorithmic approach to cytohistologic diagnosis. With over 700 printed photomicrographs and a CD-ROM offering all images in a downloadable format, this is an important resource for all anatomic pathologists.

Liquid-based cytology preparations are currently the standard of care for gynecological cytology, and are being increasingly used for non-gynecological cytology. Diagnostic Liquid-Based Cytology serves as a handy guide to diagnostic cytopathology on liquid-based preparations. The authors, renowned experts in the field, provide clear, concise, and practical diagnostic guidance. This handbook equips you to achieve accurate diagnosis of most commonly and uncommonly encountered diseases in exfoliative and aspirated tissue samples from various sites. The key cytopathological features of various diseases are described. The book is lavishly illustrated with dozens of color images that depict the full range of common and rare conditions. Diagnostic Liquid-Based Cytology offers highly practical guidance and information needed to solve common diagnostic challenges in liquid-based cytology preparations. Appropriate histopathological correlations and a consideration of the possible differential diagnosis accompany the cytological findings. The book is an excellent resource not only for practicing pathologists as well as for pathologists-in-training, and will be the perfect practical resource for daily reference in the cytopathology laboratory.

Epigenetic Cancer Therapy unites issues central to a translational audience actively seeking to understand the topic. It is ideal for cancer specialists, including oncologists and clinicians, but also provides valuable information for researchers, academics, students, governments, and decision-makers in the healthcare sector. The text covers the basic background of the epigenome, aberrant epigenetics, and its potential as a target for cancer therapy, and includes individual chapters on the state of epigenome knowledge in specific cancers (including lung, breast, prostate, liver). The book encompasses both large-scale intergovernmental initiatives as well as recent findings across cancer stem cells, rational drug design, clinical trials, and chemopreventative strategies. As a whole, the work articulates and raises the profile of epigenetics as a therapeutic option in the future management of cancer.

Although general morphological features have been used to consistently identify the changes in cell ultrastructure occurring during apoptosis, as distinct from necrosis, important advances have been achieved more recently in the investigation of the cellular and molecular aspects of this process. This book brings together the latest international research on the complex subject of programmed cell death, and covers such areas as the biochemical mechanisms, introduction of DNA fragmentation, enetic regulation, and the importance of apoptosis in the immune system, particularly during T-cell development, and in cancer. The comparison of a number of common signal transduction pathways with those involved in cell growth highlights an important relationship between apoptosis and the control of cell proliferation.

The University of Florida has an ambitious goal: to harness the power of its faculty, staff, students, and alumni to solve some of society's most pressing problems and to become a resource for the state of Florida, the nation, and the world. In the upcoming decades, an unprecedented demographic shift will take place; the eighty-five and over population is projected to climb far higher than any other age group. To keep the current elderly population healthy and help prevent future generations from experiencing poor health outcomes, researchers are studying crucial connections between skeletal muscles and whole-body health. The University of Florida is at the forefront of this research, utilizing its nationally recognized excellence in the fields of muscle biology and exercise physiology to discover unique ways to preserve muscle health in the aging and those with diseases. Find out how the proteins within muscle can be manipulated to reduce recovery times for individuals who are bedridden. Learn how older, elite athletes have resisted the inevitable degeneration that comes with aging, and how intermittent fasting may help you live longer and healthier. Step inside the lab where a researcher is uncovering the origins of cancer cachexia a wasting syndrome responsible for 20 percent of cancer deaths worldwide to improve the lives of patients. The loss of skeletal muscle through disease, disuse, or aging is associated with a host of poor health outcomes, but promising new avenues of research are being studied every day at the University of Florida to make for a healthier tomorrow.

2006 marked the 50th anniversary of the discovery of the human chromosome number, a discovery that initiated human cytogenetics as a scientific discipline and set the basis for modern medical genetics. First Years of Human Chromosomes draws together the ground-breaking work of the main researchers in the key period, 1955 to 1960. The book is based on interviews with these researchers and descriptions of the major research findings, giving a strong sense of the excitement and thoughts of those involved. First Years of Human Chromosomes provides a unique record of this exciting period of scientific research and medical advance, and will appeal to all those working in the field of genetics, whether researchers or clinicians.