This new volume of Current Topics in Developmental Biology covers Stem Cells in Development and Disease. The chapters provide a comprehensive set of reviews covering such topics as the mechanisms of pluripotency in vivo and in vitro, hematopoietic stem cell development, intestinal stem cells and their defining niche, epithelial stem cells in adult skin, the mammary stem cell hierarchy, satellite cells, neural stem cells of the hippocampus, lung stem and progenitor cells in tissue homeostasis and disease, spermatogonial stem cell functions in physiological and pathological conditions, the origin, biology, and therapeutic potential of facultative adult hepatic progenitor cells, nephron progenitor cells, adult stem cell niches, cancer stem cells, pluripotency and cellular heterogeneity, and cellular mechanisms of somatic stem cell aging
Covers the area of Stem Cells in Development and DiseaseInternational board of authors Provides a comprehensive set of reviews covering such topics as Intestinal Stem Cells, Nephron Stem Cells/Progenitors, Skin epithelial Stem Cells and Lung Stem Cells

Understanding the importance and necessity of the role of autophagy in health and disease is vital for the studies of cancer, aging, neurodegeneration, immunology, and infectious diseases. Comprehensive and up-to-date, this book offers a valuable guide to these cellular processes whilst encouraging researchers to explore their potentially important connections. Volume 3 explores the role of autophagy in specific diseases and developments, including: Crohn's Disease, Gaucher Disease, Huntington's Disease, HCV infection, osteoarthritis, and liver injury. A full section is devoted to in-depth exploration of autophagy in tumor development and cancer. Finally, the work explores the relationship between autophagy and apoptosis, with attention to the ways in which autophagy regulates apoptosis, and the ways in which autophagy has been explored in Lepidoptera, elucidating the use of larval midgut as a model for such exploration. From these well-developed foundations, researchers, translational scientists, and practitioners may work to better implement more effective therapies against some of the most devastating human diseases. Volumes in the Series Volume 1: Molecular Mechanisms. Elucidates autophagy's association with numerous biological processes, including cellular development and differentiation, cancer, immunity, infectious diseases, inflammation, maintenance of homeostasis, response to cellular stress, and degenerative diseases such as Alzheimer's, Parkinson's, Huntington's, amyotrophic lateral sclerosis, and prion diseases. Volume 2: Role in General Diseases. Describes the various aspects of the complex process of autophagy in a myriad of devastating human diseases, expanding from a discussion of essential autophagic functions into the role of autophagy in proteins, pathogens, immunity, and general diseases. Volume 3: Role in Specific Diseases. Explores the role of autophagy in specific diseases and developments, including: Crohn's Disease, Gaucher Disease, Huntington's Disease, HCV infection, osteoarthritis, and liver injury, with a full section devoted to in-depth exploration of autophagy in tumor development and cancer, as well as the relationship between autophagy and apoptosis. Volume 4: Mitophagy. Presents detailed information on the role of mitophagy, the selective autophagy of mitochondria, in health and disease, by delivering an in-depth treatment of the molecular mechanisms involved in mitophagy initiation and execution, as well as the role of mitophagy in Parkinson Disease, cardiac aging, and skeletal muscle atrophy. Volume 5: Role in Human Diseases. Comprehensively describes the role of autophagy in human diseases, delivering coverage of the antitumor and protumor roles of autophagy; the therapeutic inhibition of autophagy in cancer; and the duality of autophagy's effects in various cardiovascular, metabolic, and neurodegenerative disorders. Volume 6: Regulation of Autophagy and Selective Autophagy. Provides coverage of the mechanisms of regulation of autophagy; intracellular pathogen use of the autophagy mechanism; the role of autophagy in host immunity; and selective autophagy. Volume 7: Role of Autophagy in Therapeutic Applications. Provides coverage of the latest developments in autophagosome biogenesis and regulation; the role of autophagy in protein quality control; the role of autophagy in apoptosis; autophagy in the cardiovascular system; and the relationships between autophagy and lifestyle. Volume 8: Autophagy and Human Diseases. Reviews recent advancements in the molecular mechanisms underlying a large number of genetic and epigenetic diseases and abnormalities, and introduces new, more effective therapeutic strategies, in the development of targeted drugs and programmed cell death, providing information that will aid on preventing detrimental inflammation. Volume 9: Necrosis and Inflammation in Human Diseases. Emphasizes the role of Autophagy in necrosis and inflammation, explaining in detail the molecular mechanism(s) underlying the formation of autophagosomes, including the progression of Omegasomes to autophagosomes.

Autophagy: Cancer, Other Pathologies, Inflammation, Immunity, Infection, and Aging is a complete, authoritative examination of the role of autophagy in health and disease. Understanding this phenomenon is vital for the studies of cancer, aging, neurodegeneration, immunology, and infectious diseases. Comprehensive and forward thinking, this four-volume work offers a valuable guide to cellular processes while encouraging researchers to explore their potentially important connections. Understanding the role of autophagy is critical, considering its association with numerous biological processes, including cellular development and differentiation, cancer (both antitumor and protumor functions), immunity, infectious diseases, inflammation, maintenance of homeostasis, response to cellular stress, and degenerative diseases such as Alzheimer's, Parkinson's, Huntington's, amyotrophic lateral sclerosis, and prion diseases. Cell homeostasis is achieved by balancing biosynthesis and cellular turnover. In spite of the increasing importance of autophagy in various pathophysiological conditions mentioned above, this process remains underestimated and overlooked. As a consequence, its role in the initiation, stability, maintenance, and progression of these and other diseases (e.g., autoimmune disease) remains poorly understood. This work will broaden the knowledge base of academic and clinical professors, post-doctoral fellows, graduate and medical students regarding this vital biological process. Volumes in the Series Volume 1: Molecular Mechanisms. Elucidates autophagy's association with numerous biological processes, including cellular development and differentiation, cancer, immunity, infectious diseases, inflammation, maintenance of homeostasis, response to cellular stress, and degenerative diseases such as Alzheimer's, Parkinson's, Huntington's, amyotrophic lateral sclerosis, and prion diseases. Volume 2: Role in General Diseases. Describes the various aspects of the complex process of autophagy in a myriad of devastating human diseases, expanding from a discussion of essential autophagic functions into the role of autophagy in proteins, pathogens, immunity, and general diseases. Volume 3: Role in Specific Diseases. Explores the role of autophagy in specific diseases and developments, including: Crohn's Disease, Gaucher Disease, Huntington's Disease, HCV infection, osteoarthritis, and liver injury, with a full section devoted to in-depth exploration of autophagy in tumor development and cancer, as well as the relationship between autophagy and apoptosis. Volume 4: Mitophagy. Presents detailed information on the role of mitophagy, the selective autophagy of mitochondria, in health and disease, by delivering an in-depth treatment of the molecular mechanisms involved in mitophagy initiation and execution, as well as the role of mitophagy in Parkinson Disease, cardiac aging, and skeletal muscle atrophy. Volume 5: Role in Human Diseases. Comprehensively describes the role of autophagy in human diseases, delivering coverage of the antitumor and protumor roles of autophagy; the therapeutic inhibition of autophagy in cancer; and the duality of autophagy's effects in various cardiovascular, metabolic, and neurodegenerative disorders. Volume 6: Regulation of Autophagy and Selective Autophagy. Provides coverage of the mechanisms of regulation of autophagy; intracellular pathogen use of the autophagy mechanism; the role of autophagy in host immunity; and selective autophagy. Volume 7: Role of Autophagy in Therapeutic Applications. Provides coverage of the latest developments in autophagosome biogenesis and regulation; the role of autophagy in protein quality control; the role of autophagy in apoptosis; autophagy in the cardiovascular system; and the relationships between autophagy and lifestyle. Volume 8: Autophagy and Human Diseases. Reviews recent advancements in the molecular mechanisms underlying a large number of genetic and epigenetic diseases and abnormalities, and introduces new, more effective therapeutic strategies, in the development of targeted drugs and programmed cell death, providing information that will aid on preventing detrimental inflammation. Volume 9: Necrosis and Inflammation in Human Diseases. Emphasizes the role of Autophagy in necrosis and inflammation, explaining in detail the molecular mechanism(s) underlying the formation of autophagosomes, including the progression of Omegasomes to autophagosomes.

Understanding the importance and necessity of the role of autophagy in health and disease is vital for the studies of cancer, aging, neurodegeneration, immunology, and infectious diseases. Comprehensive and forward thinking, these books offer a valuable guide to both cellular processes while inciting researchers to explore their potentially important connections. Considering that autophagy is associated with numerous biological processes, including cellular development and differentiation, cancer (both antitumor and protumor functions), immunity, infectious diseases, inflammation, maintenance of homeostasis, response to cellular stress, and degenerative diseases such as Alzheimer's, Parkinson's, Huntington's, amyotrophic lateral sclerosis, and prion diseases, there is a great need to understanding its role. Cell homeostasis is achieved by balancing biosynthesis and cellular turnover. In spite of the increasing importance of autophagy in various pathophysiological situations (conditions) mentioned above, this process remains underestimated and overlooked. As a consequence, its role in the initiation, stability, maintenance, and progression of these and other diseases (e.g., autoimmune disease) remains poorly understood. Volumes in the Series Volume 1: Molecular Mechanisms. Elucidates autophagy's association with numerous biological processes, including cellular development and differentiation, cancer, immunity, infectious diseases, inflammation, maintenance of homeostasis, response to cellular stress, and degenerative diseases such as Alzheimer's, Parkinson's, Huntington's, amyotrophic lateral sclerosis, and prion diseases. Volume 2: Role in General Diseases. Describes the various aspects of the complex process of autophagy in a myriad of devastating human diseases, expanding from a discussion of essential autophagic functions into the role of autophagy in proteins, pathogens, immunity, and general diseases. Volume 3: Role in Specific Diseases. Explores the role of autophagy in specific diseases and developments, including: Crohn's Disease, Gaucher Disease, Huntington's Disease, HCV infection, osteoarthritis, and liver injury, with a full section devoted to in-depth exploration of autophagy in tumor development and cancer, as well as the relationship between autophagy and apoptosis. Volume 4: Mitophagy. Presents detailed information on the role of mitophagy, the selective autophagy of mitochondria, in health and disease, by delivering an in-depth treatment of the molecular mechanisms involved in mitophagy initiation and execution, as well as the role of mitophagy in Parkinson Disease, cardiac aging, and skeletal muscle atrophy. Volume 5: Role in Human Diseases. Comprehensively describes the role of autophagy in human diseases, delivering coverage of the antitumor and protumor roles of autophagy; the therapeutic inhibition of autophagy in cancer; and the duality of autophagy's effects in various cardiovascular, metabolic, and neurodegenerative disorders. Volume 6: Regulation of Autophagy and Selective Autophagy. Provides coverage of the mechanisms of regulation of autophagy; intracellular pathogen use of the autophagy mechanism; the role of autophagy in host immunity; and selective autophagy. Volume 7: Role of Autophagy in Therapeutic Applications. Provides coverage of the latest developments in autophagosome biogenesis and regulation; the role of autophagy in protein quality control; the role of autophagy in apoptosis; autophagy in the cardiovascular system; and the relationships between autophagy and lifestyle. Volume 8: Autophagy and Human Diseases. Reviews recent advancements in the molecular mechanisms underlying a large number of genetic and epigenetic diseases and abnormalities, and introduces new, more effective therapeutic strategies, in the development of targeted drugs and programmed cell death, providing information that will aid on preventing detrimental inflammation. Volume 9: Necrosis and Inflammation in Human Diseases. Emphasizes the role of Autophagy in necrosis and inflammation, explaining in detail the molecular mechanism(s) underlying the formation of autophagosomes, including the progression of Omegasomes to autophagosomes.

Autophagy in Health and Disease, Second Edition provides a comprehensive overview of the process of autophagy and its impact on human physiology and pathophysiology. It expands on the scope of the first edition by covering a wider range of cell types, developmental processes, and organ systems. The second edition is an international effort by investigators from 15 different countries whose many contributions are comprised in 28 chapters organized into six sections. The first section (Chapters 1-7) covers foundational concepts, including history, trajectory of the research field, mechanisms of autophagy, and autophagy regulation. The second section (Chapters 8-11) details developmental aspects, including stem cells, embryogenesis, hematopoiesis, and paligenosis. The subsequent sections are devoted to the role of autophagy in specific organ systems involved in metabolic control and diabetes (Chapters 12-15), the cardiovascular system (Chapters 16-18), and the nervous system (Chapters 19-20). The final section (Chapters 21-28) addresses autophagy in other organ systems vital to human health and longevity. Also included are chapters on microautophagy, chaperone-mediated autophagy, and the potential for autophagy as a therapeutic target. Autophagy in Health and Disease is invaluable to anyone new to the field as well as established investigators looking for a broader understanding of autophagy from outside their specific field of study.

The rapid expansion of the area of free radical biology in the last 25 years has occurred within a framework of assumptions and preconceived notions that has at times directed the course of this movement. The most dominant of these notions has been the view that free radical production is without exception a bad thing, and that the more efficient our elimination of these toxic substances, the better off we will be. The very important observation by Bernard Babior and colleagues in 1973 that activated phagocytes produce superoxide in order to kill micro organisms, served to illustrate that constructive roles are possible for free radicals. For many in the field, however, this merely underscored the deadly nature of oxygen-derived radicals, both from the microbe's point of view and from the host's as well. (Phagocyte-produced superoxide is responsible in part for the tissue injury manifested as inflammation. See Harris and Granger, Chapter 5, and Leff, Hybertson and Repine, Chapter 6.)
Mother Nature, however, has a penchant for being able to make a silk purse from a sow's ear. If one is dealt a bad hand, one must simply make the best of it. After two decades of focusing on the destructive side of free radicals, the last few years have begun to reveal a new and finer perspective on free radical metabolism - a role in regulation of cellular function (see Schulze-Osthoff and Baeuerle, Chapter 2). Evidence from a number of sources suggests that an increase in the oxidative status of cell encourages that cell to grow and divide. Increasing the expression of mangnese superoxide dismutase can suppress the malignant phenotype of melanon cells (see Oberley and Oberley, Chapter 3). Oxidative stress beyond a certain poitosis (from the Greek, literally "to fall apart"). Is this suicide response an evolutionary fail-safe device to curtail tumorogenesis? Does oxidative stress-induced apoptosis account for the loss of immune cells in AIDS (see Flores and McCor Chapter 4)?
This volume attempts to present the spectrum of roles, both good and bad played by active oxygen species as understood at this point in the evolution of this field of free radical biology.

International Review of Cytology presents current advances and comprehensive reviews in cell biology both plant and animal. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research. Articles in this volume include Function and Evolution of the Vacuolar Compartment in Green Algae and Land Plants
(Viridiplantae); Cell biology and pathophysiology of diacylglycerol kinase family: morphological aspects in tissues and organs; Structure and function of desmosomes; Subepithelial Fibroblasts in Intestinal Villi: Roles in Intercellular Communication; and Syndrome of Aluminum Toxicity and Diversity of Aluminum Resistance in Higher Plants.

International Review of Cytology presents current advances and comprehensive reviews in cell biology both plant and animal. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research. Articles in this volume include Mechanisms of Gradient Detection: A Comparison of Axon Pathfinding with Eukaryotic Cell Migration; Leptin and the Regulation of Hypothalamic-Pituitary-Adrenal Axis; Focal Adhesion and p53 Signaling in Cancer Cells; Cell and Molecular Biology of the Spindle Matrix; Mitochondrial Biology and Disease in Dictyostelium; Oxytocin and the Human Prostate in Health and Disease.

A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases.

International Review of Cytology presents current advances and comprehensive reviews in cell biology both plant and animal. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research.

There have been tremendous strides in cellular transplantation in recent years, leading to accepted practice for the treatment of certain diseases, and use for many others in trial phases. The long history of cellular transplantation, or the transfer of cells from one organism or region of the body to another, has been revolutionized by advances in stem cell research, as well as developments in gene therapy.
Cellular Transplants: From Lab to Clinic provides a thorough foundation of the basic science underpinning this exciting field, expert overviews of the state-of-the-art, and detailed description of clinical success stories to date, as well as insights into the road ahead. As highlighted by this timely and authoritative survey, scale-up technologies and whole organ transplantation are among the hurdles representing the next frontier.
The contents are organized into four main sections, with the first covering basic biology, including transplant immunology, the use of immunosuppressive drugs, stem cell biology, and the development of donor animals for transplantation. The next part looks at peripheral and reconstructive applications, followed by a section devoted to transplantation for diseases of the central nervous system. The last part presents efforts to address the key challenges ahead, such as identifying novel transplantable cells and integrating biomaterials and nanotechnology with cell matrices.
- Provides detailed description of clinical trials in cell transplantation
- Review of current therapeutic approaches
- Coverage of the broad range of diseases addressed by cell therapeutics
- Discussion of stem cell biology and its role in transplantation

The acclaimed International Review of Cytology series presents current advances and reviews in cell biology, both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research.

International Review of Cytology presents current advances and comprehensive reviews in cell biology--both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research.

International Review of Cytology presents current advances and comprehensive reviews in cell biology--both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research.
Key Features
* Authored by some of the foremost scientists in the field
* Provides up-to-date information and directions for future research
* Valuable reference material for advanced undergraduates, graduate students and professional scientists

This book covers core and emerging in vitro and in vivo protocols used to study how various components of the tumor microenvironment are established and subsequently interact with tumor cells to facilitate carcinogenesis. In addition, the book examines research topics including cellular and molecular biology approaches, in vivo genetic approaches, various "omics"-based strategies, therapeutic strategies to target the microenvironment, and, finally, advanced techniques in the fields of tissue engineering and nanotechnology. Written and validated in the laboratories of a number of trusted collaborating authors for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, The Tumor Microenvironment: Methods and Protocols constitutes a compendium of techniques now available to a broad audience, including basic and clinician scientists, systems biologists, and biological engineers.

This book, part contributed volume, part proceedings, discusses state-of-the-art advances on human cell transformation in cell models for the study of cancer and aging. Several of the chapters are from the Human Cell Transformation: Advances in Cell Models for the Study of Cancer and Aging conference that was held in June 2018 at McGill University. The authors represent international expertise on a wide variety of topics ranging from different types of cancer (prostate, bone, breast, etc.) to tumor microenvironment, tumor progression, homogeneity, and possible therapies and treatments.

Considerable advances have taken place since the initial isolation and characterization of human embryonic stem (HES) cells; however, significant challenges remain before their potential for restoration and regeneration processes in patients can be realized. Understanding the diversity amongst HES cell lines and realizing the ability to isolate lines with robust differentiation potential remain difficult. In the Human Embryonic Stem Cells Handbook, experts in the field provide an assortment of protocols that have been used by various laboratories around the world so as to allow both novices and experienced investigators to compare and contrast different approaches to HES cell isolation and characterization with the hope that, from these protocols, researchers might standardize approaches for HES cell biology. Written in the Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and accessible, Human Embryonic Stem Cells Handbook serves as a valuable reference for scientists pursuing this vital field and its enormous potential.

A major revision of the leading textbook of FNA cytology Both text and illustrations provide a systematic and logical approach to the acquisition, interpretation and diagnosis of Fine Needle Aspirate biopsy specimens. Orell's FNA Cytology will assist in establishing diagnostic criteria and results in the examination of benign and malignant condition in all body systems where the technique is currently applied. The information is always presented in a clinical context: it details the role of FNA and other ancillary techniques in the investigative sequence (the cytological findings are always set in the context of the patient's clinical history, physical exam and other laboratory tests). It gives practical, technical advice on how to obtain good smears and alerts the reader as to possible diagnostic pitfalls and complications. The contents are organized in a framework of anatomical regions (rather than histopathological classifications) in order to reflect the way problems present in clinical practice. Each chapter is divided into two parts: the first deals with indications, accuracy, techniques and complications; the second illustrates the cytological patterns and their implication. Both common and rare conditions are covered.Provides practical tips and advice on how to avoid pitfalls and ensure accurate diagnoses. Over 1,200 colour illustrations capture each entity's cellular, morphological and immunohistochemical appearance. Chapters have been up-dated and revised and a brand new one on cytological findings in infectious diseases added. Both MGG and Pap smears illustrated in parallel as well as the corresponding histology to help provide side-by-side analysis. Access the full text online and download images via Expert Consult. Brand new chapter on cytological findings in infectious diseases. Inclusion of immuno-profiles and other relevant ancillary tests. New illustrations. New contributing authors. Available online via Expert Consult.

Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. Thisnew edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay.
* Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays
* Discusses many new assays developed since the previous edition
* Presents recent developments in cytometric intrumentation/technology"

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.

Cell Therapy for Brain Injury is a thorough examination of using state-of-the-art cell therapy in the treatment of strokes and other traumatic brain injuries. This invaluable book covers this niche topic in depth from basic stem cell biology and principles of cell therapy through proposed mechanisms of action of cell therapy in stroke, pre-clinical data in stroke models, ongoing clinical trials, imaging and tracking of cells with MRI, neural stem cells in stroke and the "big pharma" perspective of cell therapy. Each chapter is written by well-known leaders in each field, thus providing a wealth of expertise. The breadth of this book makes it essential reading for neuroscientists, stem cell biologists, researchers or clinical trialists at pharmaceutical or biotechnology companies. It also serves as a thorough introduction for graduate students or post-doctoral fellows who hope to work in these fields.

Interest in the therapeutic value of embryonic, fetal and adult stem cell types is rapidly expanding throughout the scientific community. The first half of this century should see an explosion of therapeutic applications of stem cells which will grow from the cells and techniques described in this book. Stem Cell Culture provides a comprehensive resource for researchers in the fields of embryonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentiation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Chapters focus on the practical do's and don'ts of isolating and culturing these cell types, and use illustrative data or diagrams that allow the reader to confidently apply techniques and make this a standard reference.
*Compares and contrasts a broad range of species and stem cell types.
*Describes techniques in stem cell research, and goes beyond just protocols by presenting methods within perspective of recent literature reviews.
*Delineates critical steps and potential pitfalls for each method, provided by at-the-bench authors for expert information.
*Covers specific procedures in dealing with Human Embryonic Stem Cells, but also emphasizes other sources of potentially therapeutic stem cells such as amniotic fluid, adult muscle and adipose tissue, and umbilical cord.