Advances in our understanding of biological mechanisms have frequently been associated with the development of techniques. In situ hybridisation is a classic case of just such an advance. The technique effectively combines histochemistry with molecular biology and enables the rapid analysis of the distribution of RNA, or DNA, in the tissues. The information gained from this has caused something of a revolution in our understanding of developmental biology, since a fundamental aspect of development is the spatial and temporal expression of genes. In addition the technique has found application in the field of medicine, providing insights into the functioning of healthy tissues and the diagnosis and study of diseases. This book brings together contributions from leaders in the application of in situ hybridisation and guides the would-be exponent through the various options and variations of the technique.

This manual describes the methodology and applications of microinjection, a powerful technique for transposing material into living cells through needle injection. Information is presented on the techniques principles, the required equipment, preparation of receiving cells and injected material, and the analysis of results. Detailed protocols for a wide range of applications in different cellular systems are included. A large part of the book is also dedicated to the Xenopus laevis oocyte system.

During animal development the descendants of a single cell form many different tissues and organs in appropriate positions within an embryo. To do this they must recognise their position, and our knowledge of how this is done is examined in this book. It starts by considering how much spatial pattern is already laid down when the egg forms inside the mother, and ends just before the formation of visible organs. Within these limits it considers evidence obtained by a variety of techniques, both experimental and biochemical, and from the embryos of many different animal groups. This breadth of coverage and the amount of detail afforded, particularly to the experimental studies, distinguish it from competing works and will make it an interesting review. Moreover, in the final chapter the author analyzes this evidence in ways which will be new to most readers, and which call into question current ideas about spatial determination.

Morphogenesis, the set of processes that generate shape and form in the embryo, remains a central area in developmental biology. This book, first published in 1990, provides an overview of the events and mechanisms of morphogenesis, reviewing the major molecular and cellular mechanisms underlying morphogenetic change and describing how these processes are integrated during normal development. Most of the major embryological systems, both vertebrate and invertebrate, are discussed, with extensive coverage of the molecular mechanisms of morphogenesis involving the extracellular matrix, the membrane and the cytoskeleton. The major focus of the book, however, is on those properties of mesenchymal and epithelial cells responsible for organogenesis, while the extensive reference cover of the historical and contemporary literature (more than 500 titles) provides a useful research tool in this very wide field. This book is aimed at both scholars in the field of embryology, and postgraduate and final-year students in development and anatomy.

The phenomenon of sexual reproduction in eukaryotes poses a special problem: with each union of gametes, how does the species avoid a doubling of chromosome numbers? The solution that eukaryotes invented, early in their evolutionary history, is a unique form of nuclear division, meiosis. Meiosis serves to reduce chromosome numbers by a factor of two in the gamete or gamete-forming cells, with the result that each gamete (or gamete precursor) has a single copy of each chromosome, the haploid state; the union of gametes then restores the pre-gametic or diploid chromosome number. In this book, Bernard John presents a complete review of meiosis, describing its mechanics, position in the life cycle in different species, genetics and, finally, its significance in eukaryotic evolution. For students and researchers interested in eukaryotic cellular biology and genetics, this book may prove useful. Upper division students and research workers in genetics, cytology, and cell biology will also be able to refer to this book.

Patterns in Plant Development offers an introduction to the development of the whole plant. It is essentially a factual book that describes the complex phenomena of development in vascular plants. The point of view is structural, and emphasis is placed on the experimental approach to development. The book deals with lower vascular plants (e.g. ferns) as well as seed plants, so that the treatment of the plant, beginning with the embryo and continuing through the phase of secondary growth (the vascular cambium) is presented. The book is written so that anyone who has completed a basic first-year university course in biology or botany will be able to use it without difficulty. Sufficient background information is provided so that the reader is not required to have an extensive technical background.

Patterns in Plant Development offers an introduction to the development of the whole plant. It is essentially a factual book that describes the complex phenomena of development in vascular plants. The point of view is structural, and emphasis is placed on the experimental approach to development. The book deals with lower vascular plants (e.g. ferns) as well as seed plants, so that the treatment of the plant, beginning with the embryo and continuing through the phase of secondary growth (the vascular cambium) is presented. The book is written so that anyone who has completed a basic first-year university course in biology or botany will be able to use it without difficulty. Sufficient background information is provided so that the reader is not required to have an extensive technical background.

Coverage of the field in Instant Notes in Developmental Biology is current and focuses largely on the principles of embryonic development. It is designed to provide a clear summary of the principles of developmental biology in a compact and easily manageable structure.

The technique of allometry investigates the effects of size on such variables as food intake, energy requirements, growth rates and age at first reproduction. This book, now available in paperback, brings together much of what is known about the consequences of size and provides a new and mathematically rigorous framework within which many quantitative predictions are made and tested using published and unpublished data. New explanations are proposed for many previously unexplained phenomena such as why in some species females are thousands of times heavier than males, whereas in no species are males more than about eight times heavier than females. The models presented afford a new synthesis of the effects of size and open up more pathways for further theoretical investigation and experimental testing. Care has been taken to give verbal presentations of all the mathematical conclusions to ensure that the text is widely intelligible.

A light-hearted look at the nature of academic science, intended for anyone interested in biology but particularly for biology students who want to find out what the future holds in store. The "Egg" of the title refers to the science of developmental biology, which is the speciality of the author, and which provides the material for many of the anecdotes. The "Ego" relates to the vanity of the scientists themselves. Academic scientists have to struggle to maintain their research funding. To do this they must persuade other scientists that they are very good, and that means working at a good institution, publishing papers in the most fashionable journals and giving lectures at the most prestigious meetings. Success often goes to those with the largest egos and it is their style of operation that is described in this book. The author is a well-known scientist who has worked at both universities and research institutes. He has published over 100 scientific papers and an influential book about embryonic development: "From Egg to Embryo".

The editors present, in a single volume, a valuable reference source for students of mammalian embryology, genetics, and physiology, this book provides a thorough overview of the field of embryonic development - its current status along with possible directions in the future.

Ever wondered why your life and health can sometimes be so hard to control? Or why it seems so easy for other people? Mark Hanson and Lucy Green draw on their years of experience as scientists and educators to cut through the usual information on genetics and lifestyle to reveal the secrets of early development which start to make each of us unique, during our first 1,000 days from the moment of conception. Some surprising discoveries, based on little-known new research, show how events during our first 1,000 days make each of us who we are and explain how we control our bodies, processes that go way beyond just the genes which we inherited. Provoking new ways of thinking about being parents, this book empowers individuals and society to give the next generation the gift of a good start to life and future health.

Why does nature love symmetry? In Asymmetry, Developmental Stability and Evolution, Møller and Swaddle analyse the evolutionary implications of symmetry. They advance and explain their theory that symmetry is related to genetic stability and fitness, and that symmetric individuals appear to have quantifiable and significant advantages over their asymmetric counterparts. When assessing potential mates or competitors, animals may be able to use symmetry as an honest indication of quality. This interdisciplinary book, with its associated Web-site, will be of interest to students and researchers in the fields of ecology, evolutionary biology, genetics, and animal behaviour.

This description of a model system for cell differentiation and organogenesis is written by one of the foremost researchers in the area. The main emphasis is on the mammalian kidney, but the book also deals with the development of the transien excretory organs. It includes discussions of induction, proliferation, early cytodifferentiation and morphogenesis and organogenesis. This authoritative account will be valuable to developmental biologists and also to scientists working in paediatric nephrology. As it gives the background of normal development and of control systems, it will also be of use to nephrologists working on abnormalities in the urinary tract.

Regulation of the Early Development of the Nervous System by Growth Factors; P.F. Bartlett, et al. Polysialic Acid as a Regulator of CellCell Interactions during Muscle Innervation; U. Rutishauser. The O2A Cell Lineage; G. Levi, C. Agresti. Cell Fate in the Cerebral Cortex; J. Price. Hox Genes and the Development of the Branchial Region; P. Hunt, et al. A General Model of Cortical Histogenesis V.S. Caviness, Jr., T. Takahashi. The Use of Experimental Genetics to Study Pattern Formation in the Mammalian CNS; K. Herrup. Comparative and Cellular Aspects of Development of Connections in Cerebral Cortex; G.M. Innocenti. The Developing Mouse Whiskerpad; W. Ourednik, et al. Two Phases of Pattern Formation in the Developing Rodent Trigeminal System; S. Jhaveri, R. Erzurumlu. Andogen-Dependent Plasticity of a Neuromuscular System; C. Cracco, A. Vercelli. 7 additional articles. Index.

This book considers in detail the mechanisms of a major human problem. Chromosome imbalance affects all stages of life in ways ranging from spontaneous abortion and retardation to behavioural problems and malignancy. Charles J. Epstein concerns himself with how and why a particular chromosome imbalance produces a specific phenotype. His fundamental goal is to connect chromosome aberrations with functional abnormalities in terms of gene expression, developmental and cell biology, and metabolism. Through his examination of this relationship, we learn more about normal development and function. The book begins with an exploration of several human autosomal aneuploid phenotypes, with particular emphasis on the relationship between genotype and phenotype. In the next part, broad theoretical considerations of the mechanisms which generate these phenotypes are examined with reference to studies on man and other organisms such as bacteria and mice. Experimental approaches to study the effects of aneuploidy are presented next with special attention paid to the development of model systems for studying human aneuploidy.

Gene Regulatory Networks, Volume 139 in the Current Topics in Developmental Biology series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Topics in this release include Mouse hindbrain GRN, Xenopus endoderm GRN - organogenesis, Vertebrate limb GRN, The notochord gene regulatory network in chordate evolution: conservation and divergence from Ciona to vertebrates, Ciona early embryo GRNs, Boolean logic models, Modeling GRN response to morphogen gradient, GRN architecture, Theory of GRN evolution, Evolution of fly segmentation GRNs, GRN evolution in echinoderms, Evolution of network specificity, and more.

Methods in Cell Biology, Volume 158, the latest release in this series, highlights new advances in the field, with this release covering How to orient cells in micro-cavities for high resolution imaging of cytokinesis and lumen formation, A body-on-a-chip (BOC) system for studying gut-liver interaction, Manipulating cultured mammalian cells for mitosis research, Live-cell FLIM-FRET using a commercially available system, A comparative analysis of methods to measure kinetochore-microtubule attachment stability, A workflow for visualizing human cancer biopsies using large-format electron microscopy, Isolation of stage-specific germ cells using facs in drosophila germarium, Computational analysis of filament polymerization dynamics in cytoskeletal networks, and more.

The studies described here were carried out in the Neuroregul ation Group, Department of Physiology, University of Leiden, the Netherlands. Over the last decade, this group, in close collaboration with the Department of Neurosurgery of the Academic Hospital of Leiden, has studied the development of the central nervous system from a neuroanatomical as well as a clinical perspective. During this period, the expression of several morphore gulators in the developing rat spinal cord was extensively investigated. Parallel studies focused on the development of the spinal cord fiber systems, which was studied by means of the intrauterine use of neuronal tracers. The main goal of these studies was to extend our knowledge about the (normal) generation of the spinal cord and to contribute to the under standing of clinical problems related to regeneration and degeneration in the mammalian central nervous system. The studies on morphoregulators, in particular, appeared to benefit two different scientific areas. Firstly, the correlation between morphoregulator expression patterns and known anatomy contributed to our knowledge about spinal cord development. Secondly, the correlation between morpho regulator expression patterns and known developmental processes may help to understand their precise function(s). This volume of Advances in Anatomy, Embryology and Cell Biology presents these particular studies on the development of the rat spinal cord performed over the last decade. As well as integrating the results of the tracer studies, this volume also provides an update on the development of the rat spinal cord.

This unique book looks at the biology of aging from a fundamentally new perspective, one based on evolutionary theory rather than traditional concepts which emphasize molecular and cellular processes. The basis for this approach lies in the fact that natural selection, as a powerful determining force, tends to decline in importance with age. Many of the characteristics we associate with aging, the author argues, are more the result of this decline than any mechanical imperative contained within organic structures. This theory in turn yields the most fruitful avenues for seeking answers to the problem of aging, and should be recognized as the intellectual core of gerontology and the foundation for future research. The author ably surveys the vast literature on aging, presenting mathematical, experimental, and comparative findings to illustrate and support the central thesis. The result is the first complete synthesis of this vital field. Evolutionary biologists, gerontologists, and all those concerned with the science of aging will find it a stimulating, strongly argued account.

In this book, Eric Charnov uses ideas about symmetry, invariance, and scaling laws to explore aspects of population dynamics and the evolution of male and female life histories. Charnov's work is internationally known and will attract great interest among postgraduate students and researchers in ecology, evolution, behaviour, and many other fields within organismal biology.

These Notes grew from my research in evolutionary biology, specifically on the theory of evolutionarily stable strategies (ESS theory), over the past ten years. Personally, evolutionary game theory has given me the opportunity to transfer my enthusiasm for abstract mathematics to more practical pursuits. I was fortunate to have entered this field in its infancy when many biologists recognized its potential but were not prepared to grant it general acceptance. This is no longer the case. ESS theory is now a rapidly expanding (in both applied and theoretical directions) force that no evolutionary biologist can afford to ignore. Perhaps, to continue the life-cycle metaphor, ESS theory is now in its late adolescence and displays much of the optimism and exuberance of this exciting age. There are dangers in writing a text about a theory at this stage of development. A comprehensive treatment would involve too many loose ends for the reader to appreciate the central message. On the other hand, the current central message may soon become obsolete as the theory matures. Although the restricted topics I have chosen for this text reflect my own research bias, I am confident they will remain the theoretical basis of ESS theory. Indeed, I feel the adult maturity of ESS theory is close at hand and I hope the text will play an important role in this achievement.

The North American opossum (Didelphis virginiana) generally is regarded as an important animal, phylogenetically. It is considered to represent a prototype marsupial and closely resembles fossil didelphids (Tyndale-Biscoe 1973). Numerous studies concerning the reproductive biology, embryology, and neurobiology of the opossum have been published. More recently, Didelphis has become popular as an animal model for gastroenterological studies because of the remarkable anatomical and physiological similarities of the esophagus as compared to that of man. Most of the studies of early development have concentrated on early cleavage stages and the formation of the three primary germ layers (Hartman 1916, 1919) and fetal membranes (Selenka 1887; McCrady 1938). The ova of Didelphis remain in the oviduct only for about 24 h before entering the uterus. A corona radiata is absent and each oocyte is surrounded only by a perivitel- line space and a zona pellucida (Talbot and DiCarlantonio 1984). During the short transit period, the egg is fertilized by a single spermatozoon (Rodger and Bedford 1982a,b).

During the last four decades remarkable developments have taken place in instrumentation and techniques for characterizing the microstructure and microcomposition of materials. Some of the most important of these instruments involve the use of electron beams because of the wealth of information that can be obtained from the interaction of electron beams with matter. The principal instruments include the scanning electron microscope, electron probe x-ray microanalyzer, and the analytical transmission electron microscope. The training of students to use these instruments and to apply the new techniques that are possible with them is an important function, which. has been carried out by formal classes in universities and colleges and by special summer courses such as the ones offered for the past 19 years at Lehigh University. Laboratory work, which should be an integral part of such courses, is often hindered by the lack of a suitable laboratory workbook. While laboratory workbooks for transmission electron microscopy have-been in existence for many years, the broad range of topics that must be dealt with in scanning electron microscopy and microanalysis has made it difficult for instructors to devise meaningful experiments. The present workbook provides a series of fundamental experiments to aid in "hands-on" learning of the use of the instrumentation and the techniques. It is written by a group of eminently qualified scientists and educators. The importance of hands-on learning cannot be overemphasized.

The nervous system is particularly fascinating for many biologists because it controls animal characteristics such as movement, behavior, and coordinated thinking. Invertebrate neurobiology has traditionally been studied in specific model organisms, whilst knowledge of the broad diversity of nervous system architecture and its evolution among metazoan animals has received less attention. This is the first major reference work in the field for 50 years, bringing together many leading evolutionary neurobiologists to review the most recent research on the structure of invertebrate nervous systems and provide a comprehensive and authoritative overview for a new generation of researchers. Presented in full colour throughout, Structure and Evolution of Invertebrate Nervous Systems synthesizes and illustrates the numerous new findings that have been made possible with light and electron microscopy. These include the recent introduction of new molecular and optical techniques such as immunohistochemical staining of neuron-specific antigens and fluorescence in-situ-hybridization, combined with visualization by confocal laser scanning microscopy. New approaches to analysing the structure of the nervous system are also included such as micro-computational tomography, cryo-soft X-ray tomography, and various 3-D visualization techniques. The book follows a systematic and phylogenetic structure, covering a broad range of taxa, interspersed with chapters focusing on selected topics in nervous system functioning which are presented as research highlights and perspectives. This comprehensive reference work will be an essential companion for graduate students and researchers alike in the fields of metazoan neurobiology, morphology, zoology, phylogeny and evolution.