4 Water Sources ........................................ 149 Criteria ............................................. 149 Major types .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150 . . . . . . . . . . . . Summary ............................................ 152 5 Water Treatment ...................................... 155 Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 . . . . . . . . . . . . Materials ............................................ 155 Treatment options . . . . . . . . . . . . . . . . . . . . . . . . . . . 156 . . . . . . . . . . . System design ........................................ 169 System monitoring and control . . . . . . . . . . . . . . . . . . . . . 172 . . . . . . . . Environmental considerations .............................. 174 Summary ............................................ 174 6 Culture Units ......................................... 175 Considerations in choosing culture units ...................... 175 Characteristics of culture units . . . . . . . . . . . . . . . . . . . . . 175 . . . . . . . . Applications of culture units .............................. 191 Hatchery design " . . . . . . . . . . . . . . . . . . . . . . . . . . 208 . . . . . . . . . . . Summary ............................................ 210 7 Obtaining Fish for Stocking . ............................. 211 Stock from the wild .................................... 211 Stock from the hatchery ................................. 211 Spermatogenesis (sperm formation) ....................... 232 Oogenesis (egg formation) ............................. 232 Oocyte maturation ................................... 233 Endocrine control of oocyte maturation and ovulation .......... 237 fuduced ovulation . . . . . . . . . . . . . . . . . . . . . . . . . . 238 . . . . . . . . . . Timing and egg quality . . . . . . . . . . . . . . . . . . . . . . . 257 . . . . . . . . . Artificial fertilization ................................. 265 Care of eggs ....................................... 267 Storage of gametes ................. ' .................. 269 Natural ovulation . . . . . . . . . . . . . . . . . . . . . . . . . . 270 . . . . . . . . . . Care of broodfish . . . . . . . . . . . . . . . . . . . . . . . . . . 289 . . . . . . . . . . Egg collection .. . . . . . . . . . . . . . . . . . . . . . . . . . . 290 . . . . . . . . . . fuduced vs natural ovulation ............................ 290 Broodfish adaptability . . . . . . . . . . . . . . . . . . . . . . . . . 291 . . . . . . . . . . Examples ............................................ 291 Genetic considerations . . . . . . . . . . . . . . . . . . . . . . . . . 295 . . . . . . . . . . Hybridization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 296 . . . . . . . . . . . . Sex control .......................................... 296 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 298 . . . . . . . . . . . . . vi 8 Nutrition of Larval Fish . . . . . . . . . . . . . . . . . . . . . . . 299 . . . . . . . . . . Feeding criteria ....................................... 299 Choice and culture of foods . . . . . . . . . . . . . . . . . . . . . . 307 . . . . . . . . . General feeding practices . . . . . . . . . . . . . . . . . . . . . . . 336 . . . . . . . . . . Specific feeding practices ................................ 352 General methods used in our hatchery . . . . . . . . . . . . . . . . . 372 . . . . . . . Industrial-scale larval food processing in Italian hatcheries ......... 373 Summary ............................................ 374 9 Nutrition of Juvenile and Adult Fish ...................... 375 ............................. 375 Requirements and components Broodstock nutrition .................................... 407 Nutritional disorders .................................... 408 Environmental considerations . . . . . . . . . . . . . . . . . . . . . 411 . . . . . . . . . Feed studies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 411 . . . . . . . . . . . . Suggested feed formulas ................................. 460 Making and storing feeds ................................ 461 Feeding methods ...................................... 464 Summary ............................................ 467 10 Energetics ............................................ 469 Energy budget components and influencing factors . . . . . . . . . . . 469 . . . .

Advances in morphological and molecular methods continue to uncover new information on the origin and evolution of bats. Presenting some of the most remarkable discoveries and research involving living and fossil bats, this book explores their evolutionary history from a range of perspectives. Phylogenetic studies based on both molecular and morphological data have established a framework of evolutionary relationships that provides a context for understanding many aspects of bat biology and diversification. In addition to detailed studies of the relationships and diversification of bats, the topics covered include the mechanisms and evolution of powered flight, evolution and enhancement of echolocation, feeding ecology, population genetic structure, ontogeny and growth of facial form, functional morphology and evolution of body size. The book also examines the fossil history of bats from their beginnings over 50 million years ago to their diversification into one of the most globally wide-spread orders of mammals living today.

While it is true that members of most sexually reproducing species can be defined as either male or female, those who belong to the rest of the biological world are not so simply understood. Hermaphroditic creatures reproduce both as male and as female individuals, providing a fascinating glimpse into alternative sexual practices in nature and their ecological and evolutionary successes and failures.

Eloquently written by an award-winning biologist and pioneer in molecular ecology, this primer on hermaphroditism traces the phenomenon throughout Earth's myriad species, accounting for the adaptive significance of alternative sexual systems. Accessible and richly illustrated, the text maps the evolutionary origins of hermaphroditism, as well as its historical instances and fictional representations, underscoring the relevance of dual sexuality to our biological, intellectual, and cultural making. John C. Avise describes the genetics, ecology, phylogeny, and natural history of hermaphroditic plants, fish, and invertebrate animals and details organisms that either reproduce simultaneously as male and female or switch routinely between one sex and the other. Filled with surprising creatures and compelling revelations, this textbook stands alone in its clear yet comprehensive treatment of hermaphroditism and its unique challenge to the supremacy of separate sexes.

Almost a quarter of a century has elapsed since Cellular Biology of the Uterus, the predecessor of the present volume, was planned. During that period, especially in the decade since the publication of the last edition of Biology of the Uterus, new information in the field has been so voluminous as to require major revisions of most of the chapters, the addition of several new chapters, and the collaboration of a second editor to facilitate the selection of appropriate experts as authors. As in prior editions, a balance has been struck between classical biology and modem biochemistry and biophysics. The inclusion of basic histological and embryo logical information provides a necessary, though often lacking, background for the protein chemist and molecular biologist and a bridge between the cell biologist and clinician. Thus, major practical problems in human reproduction, such as the genesis of endometrial carcinoma and the cause of the initiation of labor, may be approached on a firm scientific footing. The current edition deals primarily with the biology of the uterus itself (comparative and human) rather than placentation or pregnancy and thus is a synthesis of data derived from many techniques, both conventional and modem. As it is clearly beyond the competence of anyone scientist to prepare such a text on the basis of personal knowledge and experience, the aid of 22 distinguished scientists was enlisted."

Originally published during the early part of the twentieth century, the Cambridge Manuals of Science and Literature were designed to provide concise introductions to a broad range of topics. They were written by experts for the general reader and combined a comprehensive approach to knowledge with an emphasis on accessibility. The Life-Story of Insects by G. H. Carpenter was first published in 1913. The book contains an informative account of the facts and meanings of insect transformations, allowing scope for further entomological investigation in the field.

From the discovery of Pdx1, the first "master gene" of pancreatic development, to the most recent findings on the role of microRNAs in beta cell homeostasis, the last fifteen years have seen an unprecedented advance in our understanding of the precise development and organization of the many different cell types that make up the pancreas. It is now widely acknowledged that the therapeutic differentiation of stem cells into pancreatic cells is an ambitious endeavor that will not succeed without a thorough understanding of the molecular processes underlying the native development of the organ. This book, aimed at experts and students alike, offers a comprehensive review of the state of the art in both pancreatic development and regeneration. The many strategies to differentiate adult and embryonic stem cells into pancreatic beta cells are also discussed in the context of potential therapeutic interventions for type I diabetes.

Comparative endocrinology is one of the most rapidly developing subdis ciplines within the field of endocrinology, and it is having a significant impact on research at the molecular, cellular, organisma1 and environmental levels. Much of the current ferment in endocrinology is in reproductive endocrinology. The purpose of this volume on hormones and reproduction in fishes, amphibians and reptiles is to summarize our present understandings and to identify important research problems to be addressed in the area of comparative reproductive endocrinology. It was inspired by the gathering at Copper Mountain, Colorado, of eminent endocrine scientists from around the world on the occasion of the Tenth International Symposium on Comparative Endocrinology in July, 1985. While preparing for that meeting, we decided that a special volume on reproductive endocrinology was needed to summarize what is known and to stimulate research in particular directions. Why do we emphasize fishes, amphibians and reptiles? First, knowledge about the reproductive endocrinology of these ectothermic vertebrates can provide a clearer picture of the evolution of reproductive hormones and their effects on target organs. This comparative approach can lead to new theories about the evolution of reproductive control mechanisms. Second, studies concerning the reproductive endocrinology of "lower" vertebrates can result in development of "model systems" for application to studies of birds and mammals. Indeed, information about the patterns of reproductive control in ectothermic vertebrates can tell us which are evolutionarily stable and which are labile."

Cell signalling lies at the heart of many biological processes and currently is the focus of intense research interest. In multicellular organisms, it is central to how different types of cell communicate with each other and how they detect and respond to extracellular signals. Intercellular communication is vital to single-celled organisms as well, allowing them to respond to environmental cues and signals.

To date, much of the understanding of signalling mechanisms has come from research on specific cell types (eg mouse lymphocyte and cardiomyocyte) or on organisms in which communication systems such as nervous and endocrine systems are well established. This volume therefore aims to 'fill the gap' by concentrating on 'simple organisms' where the elements of those signalling systems first evolved. Many of the groups covered contain important pathogens or parasites, and the potential for manipulating signalling pathways for therapeutic intervention will be highlighted.

Biological and machine systems exist within a complex and changing three-dimensional world. We appear to have no difficulty understanding this world, but how do we go about forming a perceptual model of it? Centred around three key themes: depth processing and stereopsis; motion and navigation in 3D; and natural scene perception, this volume explores the latest cutting-edge research into the perception of three dimension environments. It features contributions from top researchers in the field, presenting both biological and computational perspectives. Topics covered include binocular perception; blur and perceived depth; stereoscopic motion in depth; and perceiving and remembering the shape of visual space. This unique book will provide students and researchers with an overview of ongoing research as well as perspectives on future developments in the field. Colour versions of a selection of the figures are available at www.cambridge.org/9781107001756.

How do we understand and explain the apparent dichotomy between plasticity and robustness in the context of development? Can we identify these complex processes without resorting to 'either/or' solutions? Written by two leaders in the field, this is the first book to fully unravel the complexity of the subject, explaining that the epigenetic processes generating plasticity and robustness are in fact deeply intertwined. It identifies the different mechanisms that generate robustness and the various forms of plasticity, before considering the functional significance of the integrated mechanisms and how the component processes might have evolved. Finally, it highlights the ways in which epigenetic mechanisms could be instrumental in driving evolutionary change. Essential reading for biologists and psychologists interested in epigenetics and evolution, this book is also a valuable resource for biological anthropologists, sociobiologists, child psychologists and paediatricians.

Originally published in 2005, this unique resource presents 27 easy-to-follow laboratory exercises for use in student practical classes in developmental biology. These experiments provide key insights into developmental questions, and many of them are described by the leaders in the field who carried out the original research. This book intends to bridge the gap between experimental work and the laboratory classes taken at the undergraduate and post-graduate levels. All chapters follow the same format, taking the students from materials and methods, through results and discussion, so that they learn the underlying rationale and analysis employed in the research. The book will be an invaluable resource for graduate students and instructors teaching practical developmental biology courses. Chapters include teaching concepts, discussion of the degree of difficulty of each experiment, potential sources of failure, as well as the time required for each experiment to be carried out in a class with students.

This book provides a comprehensive view of research in lens developmental biology, emphasising technical and molecular breakthroughs. Elucidation of the mechanisms that govern lens development has enabled us to understand how the normal lens forms and how developmental processes are involved in the maintenance of its normal structure, function and growth throughout life. This knowledge is fundamental to our understanding of many lens disorders. The ocular lens has also become a model for understanding the developmental biology of more complex organ systems. In this 2004 book, leading experts in lens cell biology and development discuss lens evolution, induction, morphology, the regulation of the lens cell cycle and fiber cell differentiation, as well as lens regeneration. This book is an authoritative treatment of the subject that will serve as a reference for graduate students and research scientists in developmental biology and in the visual sciences, as well as for ophthalmologists.

Originally published in 1995, this significant publication on genomic or parental imprinting was prepared by an outstanding team of international authorities. Genomic imprinting results in the preferential expression of one allele, depending on the parent of origin. It is associated with several disease syndromes in humans. Interest in this area has expanded rapidly from the time when it was first recognised that some aspects of inheritance were not adequately explained by the Mendelian laws. The chapters cover a wealth of material to help explain not only the mechanisms of genomic imprinting but also its biological and medical consequences. This interdisciplinary volume encompasses clinical genetics, pathology, developmental biology, evolution and genetics. It will be of interest to all scientists and clinicians working in this area.

Darwin's theory of evolution by natural selection fails to explain the forms of organisms because it focuses on inheritance and survival, not on how organisms are generated. The first part of this 2007 book (by Gerry Webster) looks critically of the conceptual structure of Darwinism and describes the limitation of the theory of evolution as a comprehensive biological theory, arguing that a theory of biological form is needed to understand the structure of organisms and their transformations as revealed in taxonomy. The second part of the book (by Brian Goodwin) explores such a theory in terms of organisms as developing and transforming dynamic systems, within which gene action is to be understood. A number of specific examples, including tetrapod limb formation and Drosophila development, are used to illustrate how these hierarchically-organized dynamic fields undergo robust symmetry-breaking cascades to produce generic forms.

Biological development, how organisms acquire their form, is one of the great frontiers in science. While a vast knowledge of the molecules involved in development has been gained in recent decades, big questions remain on the molecular organization and physics that shape cells, tissues and organisms. Physical scientists and biologists traditionally have very different backgrounds and perspectives, yet some of the fundamental questions in developmental biology will only be answered by combining expertise from a range of disciplines. This book is a personal account by Professor Lionel Harrison of an interdisciplinary approach to studying biological pattern formation. It articulates the power of studying dynamics in development: that to understand how an organism is made we must not only know the structure of its molecules; we must also understand how they interact and how fast they do so.

Originally published in 1985, this book describes research on the ecological, structural, physiological, genetic and molecular factors that control morphogenesis in the higher fungi. The topics range from the relation between organism and substrate to problems associated with the production of mushrooms in commercial conditions, and include accounts of research on biochemical, molecular and structural aspects of mushroom fruit body development. Thus both pure and applied studies of the biology of basidiomycetes are included in this volume, which provides a detailed synthesis of the area, by authors of the highest calibre.

Meristematic cells in plants become the many different types of cells found in a mature plant. This is achieved by a selective response to chemical signals both from neighbouring cells and distant tissues. It is these responses that shape the plant, its time of flowering, the sex of its flowers, its length of survival or progress to senescence and death. How do plants achieve this? This treatise addresses this question using well-chosen examples to illustrate the concept of target cells. The authors discuss how each cell has the ability to discriminate between different chemical signals, determining which it will respond to and which it will ignore. The regulation of gene expression through signal perception and signal transduction is at the core of this selectivity and the Target Cell concept. This volume will serve as a valuable reference for all researchers working in the field of plant developmental biology.

The present review covers a very neglected field in regeneration studies, namely, tissue and organ regeneration in reptiles, especially represented by the lizard model of regeneration. The term "regeneration" is intended here as "the ability of an adult organism to recover damaged or completely lost body parts or organs." The process of recovery is further termed "restitutive regeneration" when the lost part is reformed and capable of performing the complete or partial physiological activity performed by the original, lost body part. Lizards represent the only amniotes that at the same time show successful organ regeneration, in the tail, and organ failure, in the limb (Marcucci 1930a, b; Simpson 1961, 1970, 1983). This condition offers a unique opportunity to study at the same time mechanisms that in different regions of the same animal control the success or failure of regeneration. The lizard model is usually neglected in the literature despite the fact that the lizard is an amniote with a basic histological structure similar to that of mammals, and it is therefore a better model than the salamander (an a- mniote) model to investigate regeneration issues.

Maia is the story of an idea, and its development into a working hypothesis, that provides a cybernetic interpretation of how growth is controlled. Growth at the lowest level is controlled by regulating the rate of growth. Access to the output of control mechanisms is provided by perturbing the growing organism, and then filtering out the consequences to growth rate. The output of the growth control mechanism is then accessible for interpretation and modelling. Perturbation experiments have been used to provide interpretations of hormesis, the neutralization of inhibitory load and acquired tolerance to toxic inhibition, and catch-up growth. The account begins with an introduction to cybernetics covering the regulation of growth and population increase in animals and man and describes this new approach to access the control of growth processes. This book is suitable for postgraduate students of biological cybernetics and researchers of biological growth, endocrinology, population ecology and toxicology.

Branching morphogenesis, the creation of branched structures in the body, is a key feature of animal and plant development. This book brings together, for the first time, expert researchers working on a variety of branching systems to present a state-of-the-art view of the mechanisms that control branching morphogenesis. Systems considered range from single cells, to blood vessel and drainage duct systems to entire body plans, and approaches range from observation through experiment to detailed biophysical modelling. The result is an integrated overview of branching.

The objectives of this volume are to present an up-to-date (literature survey up to 2001) account of the biology of Artemia focusing particularly upon the major advances in knowledge and understanding achieved in the last fifteen or so years and emphasising the operational and functional linkage between the biological phenomena described and the ability of this unusual animal to thrive in extreme environments. Artemia is a genus of anostracan crustaceans, popularly known as brine shrimps. These animals are inhabitants of saline environments which are too extreme for the many species which readily predate them if opportunity offers. They are, thus, effectively inhabitants of extreme (hypersaline) habitats, but at the same time are able to tolerate physiologically large changes in salinity, ionic composition, temperature and oxygen tension. Brine shrimp are gener ally thought of as tropical and subtropical, but are also found in regions where temperatures are very low for substantial periods such as Tibet, Siberia and the Atacama desert. They have, thus, great powers of adaptation and are of interest for this capacity alone. The earliest scientific reference to brine shrimp is in 1756, when Schlosser reported their existence in the saltpans of Lymington, England. These saltpans no longer exist and brine shrimp are not found in Britain today. Later, Linnaeus named the brine shrimp Cancer salinus and later still, Leach used the name Artemia salina. The strong effect which the salinity of the medium exerts on the morphological development of Artemia is now widely recognised."

This book is indispensable to researchers in fields as diverse as Molecular Biology and Biophysics. It covers the important role that mitochondria play in a variety of biochemical spheres. It analyses how mitochondria affect metabolic pathways, how they are active in the regulation of cytosolic constituents, and their role in initiating signal pathways. Also covered are the way mitochondria help to regulate apoptosis, and how they modulate cellular hypertrophy and proliferation. It gives an overview of the emergence of mitochondria as an important regulator of cell signaling, with a particular focus on their pathophysiology.

In September 2005 an International Meeting on "Targeted Therapies in Cancer: Myth or Reality" was held in Milan. This successful Meeting was intended to represent a forum for scientists and clinicians working in cancer drug discovery and therapy to share their reflections and experiences on how the paradigm shift from empiricism to molecular targeted therapies is contributing to the translation of basic knowledge into new therapies for cancer patients. This book collects the contributions given by scientists and clinicians, from Academia and Industry, who participated to this Meeting.

We hope that this book contributes to improve our approach to cancer drug discovery and, ultimately, to find new, more efficacious and better tolerated drugs for cancer patients. It provides an overview of diverse approaches ranging from drug discovery to cellular therapy. Although this change in paradigm has been useful, its entry into the clinical arena was associates with unforeseen problems including the emergence of resistance, unexpected side effects and failures. Time is therefore ripe for a critical cultural reflection on the state of the art, prospects and limitations. Ultimately, is targeted therapy in cancer a myth or a reality?

Lemurs: Ecology and Adaptation brings together information from recent research, and provides new insight into the study of lemur origins, and the ecology and adaptation of both extant and recently extinct lemurs. In addition, it addresses issues of primate behavioral ecology and how environment can play a major role in explaining species variation. Moreover, in a larger context, the information contained in this volume expands our knowledge of primate ecology and allows us further insight into mammalian adaptations to unusual and often harsh environmental conditions that arise from both natural and anthropogenic factors.

The book is divided into two sections. The first section is a background to lemurs and their ecology and it includes chapters on origins of lemurs, history of ecological studies on lemurs in Madagascar, theories relating to the evolution of lemur traits, and ecology of the recently extinct (sub-fossil) lemurs. Section two is comprised of chapters focusing on the ecology and adaptations of many species of extant lemurs to the diverse habitats found on Madagascar, and in some cases, adaptations to extreme climatic variability and natural disasters.

This volume covers aspects of ecology, behavior, genetics, taxonomy, 'cultural' patterns, hunting by non-human primates, physiology, dietary chemistry, and ecotourism, in several major clades of primates from galagos and pottos, through cercopithecoids, to hominoids.