Plant biotechnology has now become a key tool in improving crop productivity and enhancing commercial value of plant products. The book complies various methods of in vitro propagation and genetic manipulation of important aromatic and medicinal plants. It puts together latest techniques and innovations in the field of plant biotechnology such as effective protocols of genetic manipulation, isolation of secondary metabolites, use of somaclonal variation, stress management in plants. It also explores the role of various physiological and biochemical factors affecting the genetic stability of in-vitro cultured plants. These themes are of interest to both graduate and postgraduate students. Further this book will be useful for to researchers, academicians and industrialist to review latest progress and future prospects of these technologies.

During the past 15 years, cellular and molecular approaches have emerged as valuable adjuncts to supplement and complement conventional breeding methods for a wide variety of crop plants. Biotechnology increasingly plays a role in the creation, conservation, characterization and utilization of genetic variability for germplasm enhancement. For instance, anther/microspore culture, somaclonal variation, embryo culture and somatic hybridization are being exploited for obtaining incremental improvement in the existing cultivars. In addition, genes that confer insect- and disease-resistance, abiotic stress tolerance, herbicide tolerance and quality traits have been isolated and re-introduced into otherwise sensitive or susceptible species by a variety of transgenic techniques. Together these transformative methodologies grant access to a greater repertoire of genetic diversity as the gene(s) may come from viruses, bacteria, fungi, insects, animals, human beings, unrelated plants or even be artificially derived. Remarkable achievements have been made in the production, characterization, field evaluation and commercialization of transgenic crop varieties worldwide. Likewise, significant advances have been made towards increasing crop yields, improving nutritional quality, enabling crops to be raised under adverse conditions and developing resistance to pests and diseases for sustaining global food and nutritional security. The overarching purpose of this 3-volume work is to summarize the history of crop improvement from a technological perspective but to do so with a forward outlook on further advancement and adaptability to a changing world. Our carefully chosen "case studies of important plant crops" intend to serve a diverse spectrum of audience looking for the right tools to tackle complicated local and global issues.

Sebacinales have emerged as a fascinating order with mutualistic plant-fungal symbionts that consists of exclusively beneficial fungi.

This volume of Soil Biology presents an overview of the current results in Sebacinales research with a focus on the potential of these fungi in crop improvement and stress tolerance. The authors demonstrate that Sebacinales are not only extremely versatile in their associations with roots, but are also almost universally present as symptomless endophytes. With this extraordinary diversity, Sebacinales with the key fungus "Piriformospora indica" might possess remarkable significance in natural ecosystems. Their biotechnological applications are expected to improve the quality of crops while maintaining ecologically and economically sustainable production systems.

This book focuses on the conventional breeding approach, and on the latest high-throughput genomics tools and genetic engineering / biotechnological interventions used to improve rice quality. It is the first book to exclusively focus on rice as a major food crop and the application of genomics and genetic engineering approaches to achieve enhanced rice quality in terms of tolerance to various abiotic stresses, resistance to biotic stresses, herbicide resistance, nutritional value, photosynthetic performance, nitrogen use efficiency, and grain yield. The range of topics is quite broad and exhaustive, making the book an essential reference guide for researchers and scientists around the globe who are working in the field of rice genomics and biotechnology. In addition, it provides a road map for rice quality improvement that plant breeders and agriculturists can actively consult to achieve better crop production.

Our lives and well being intimately depend on the exploitation of the plant genetic resources available to our breeding programs. Therefore, more extensive exploration and effective exploitation of plant genetic resources are essential prerequisites for the release of improved cultivars. Accordingly, the remarkable progress in genomics approaches and more recently in sequencing and bioinformatics offers unprecedented opportunities for mining germplasm collections, mapping and cloning loci of interest, identifying novel alleles and deploying them for breeding purposes. This book collects 48 highly interdisciplinary articles describing how genomics improves our capacity to characterize and harness natural and artificially induced variation in order to boost crop productivity and provide consumers with high-quality food. This book will be an invaluable reference for all those interested in managing, mining and harnessing the genetic richness of plant genetic resources.

"Biotechnology and Plant Breeding" includes critical discussions of the newest and most important applications of biotechnology in plant breeding, covering key topics such as biometry applied to molecular analysis of genetic diversity, genetically modified plants, and more. This work goes beyond recombinant DNA technology to bring together key information and references on new biotech tools for cultivar development, such as double-haploids, molecular markers, and genome-wide selection, among others.

It is increasingly challenging for plant breeders and agricultural systems to supply enough food, feed, fiber and biofuel for the global population. As plant breeding evolves and becomes increasingly sophisticated, a staggering volume of genetic data is now generated. "Biotechnology and Plant Breeding" helps researchers and students become familiar with how the vast amounts of genetic data are generated, stored, analyzed and applied.

This practical resource integrates information about plant breeding into the context of modern science, and assists with training for plant breeders including those scientists who have a good understanding of molecular biology/biotechnology and need to learn the art and practice of plant breeding. Plant biologists, breeding technicians, agronomists, seed technologists, students, and any researcher interested in biotechnologies applied to plant breeding will find this work an essential tool and reference for the field.
Presents in-depth but easy-to-understand coverage of topics, so plant breeders can readily comprehend them and apply them to their breeding programs Includes chapters that address the already developed and optimized biotechnologies for cultivar development, with real-world application for usersFeatures contributions by authors with several years of experience in their areas of expertise

In the pursuit of technological advancement in the field of biotechnology and pharmaceutical industries to counteract health issues, bacterial infections remain a major cause of morbidity and mortality. The ability of bacterial pathogens to form biofilms further agglomerates the situation by showing resistance to conventional antibiotics. To overcome this serious issue, bioactive metabolites and other natural products were exploited to combat bacterial infections and biofilm-related health consequences. Natural products exhibited promising results in vitro, however; their efficacy in in vivo conditions remain obscured due to their low-solubility, bioavailability, and biocompatibility issues. In this scenario, nanotechnological interventions provide a multifaceted platform for targeted delivery of bioactive compounds by slow and sustained release of drug-like compounds. The unique physico-chemical properties, biocompatibility and eco-friendly nature of bioinspired nanostructures has revolutionized the field of biology to eradicate microbial infections and biofilm-related complications. The green-nanotechnology based metal and metal oxide nanoparticles and polymeric nanoparticles have been regularly employed for antimicrobial and antibiofilm applications without causing damage to host tissues. The implications of these nanoparticles toward achieving sustainability in agriculture by providing systemic resistance against a variety of phytopathogens therefore plays crucial role in growth and crop productivity. Also the advent of smart and hybrid nanomaterials such as metal-based polymer nanocomposites, lipid-based nanomaterials and liposomes have the inherent potential to eradicate bacterial biofilm-related infections in an efficient manner. The recent development of carbon-based nanomaterials such as carbon nanotubes (CNTs) and silica based nanomaterials such as mesoporous silica nanoparticles (MSNs) also exploit a target of dreadful healthcare conditions such as cancer, immunomodulatory diseases, and microbial infections, as well as biofilm-related issues owing to their stability profile, biocompatibility, and unique physio-chemical properties. Recently novel physical approaches such as photothermal therapy (PTT) and antimicrobial photodynamic therapy (aPDT) also revolutionized conventional strategies and are engaged in eradicating microbial biofilm-related infections and related health consequences. These promising advancements in the development of novel strategies to treat microbial infections and biofilm-related multidrug resistance (MDR) phenomenon may provide new avenues and aid to conventional antimicrobial therapeutics.

This book provides insights into the latest achievements in genomics research on Brassica rapa. It describes the findings on this Brassica species, the first of the U's triangle that has been sequenced and a close relative to the model plant Arabidopsis, which provide a basis for investigations of major Brassica crop species. Further, the book focuses on the development of tools to facilitate the transfer of our rich knowledge on Arabidopsis to a cultivated Brassica crop. Key topics covered include genomic resources, assembly tools, annotation of the genome, transposable elements, comparative genomics, evolution of Brassica genomes, and advances in the application of genomics in the breeding of Brassica rapa crops.

Meeting the world's food security challenge will require a multi-national, collaborative effort to integrate the best research from science, engineering and socioeconomics so that technological advances can bring benefits where they are most needed. The present book covers the effect of major environmental problems on crop production and how to cope with these issues for sustainable agriculture and improvements of crops. The world's population is predicted to hit 9.6 Billion by 2050, up from today's total of nearly 7.3 Billion, and with it food demand is predicted to increase substantially. The post-war 'second agricultural revolution' in developed countries, and the 'green revolution' in developing nations in the mid- 1960s converted agricultural practices and elevated crop yields spectacularly, but the outcome is levelling off and will not meet projected demand. Simultaneously, crop production is affected by many other factors, including industrial pollution, overuse of fertilizers and insecticides, heavy metal and radiation stresses etc. It has been noted that many pests are becoming resistant to insecticides. Estimates vary, but around 25% of crops can be lost to pests and diseases. Climate change associated with agriculture is also a global issue. Agriculture is a significant contributor to greenhouse gases and is estimated to account for 10-12% of total greenhouse gas (GHG) emissions. Many of the issues highlighted are global problems and are addressed thoroug hly in this work.

Petunia belongs to the family of the Solanaceae and is closely related to important crop species such as tomato, potato, eggplant, pepper and tobacco. With around 35 species described it is one of the smaller genera and among those there are two groups of species that make up the majority of them: the purple flowered P.integrifolia group and the white flowered P.axillaris group. It is assumed that interspecific hybrids between members of these two groups have laid the foundation for the huge variation in cultivars as selected from the 1830 s onwards.

Petunia thus has been a commercially important ornamental since the early days of horticulture. Despite that, Petunia was in use as a research model only parsimoniously until the late fifties of the last century. By then seed companies started to fund academic research, initially with the main aim to develop new color varieties. Besides a moment of glory around 1980 (being elected a promising model system, just prior to the Arabidopsis boom), Petunia has long been a system in the shadow. Up to the early eighties no more then five groups developed classical and biochemical genetics, almost exclusively on flower color genes. Then from the early eighties onward, interest has slowly been growing and nowadays some 20-25 academic groups around the world are using Petunia as their main model system for a variety of research purposes, while a number of smaller and larger companies are developing further new varieties.

At present the system is gaining credibility for a number of reasons, a very important one being that it is now generally realized that only comparative biology will reveal the real roots of evolutionary development of processes like pollination syndromes, floral development, scent emission, seed survival strategies and the like.

As a system to work with, Petunia combines advantages from several other model species: it is easy to grow, sets abundant seeds, while self- and cross pollination is easy; its lifecycle is four months from seed to seed; plants can be grown very densely, in 1 cm2 plugs and can be rescued easily upon flowering, which makes even huge selection plots easy to handle. Its flowers (and indeed leaves) are relatively large and thus obtaining biochemical samples is no problem. Moreover, transformation and regeneration from leaf disc or protoplast are long established and easy-to-perform procedures. On top of this easiness in culture, Petunia harbors an endogenous, very active transposable element system, which is being used to great advantage in both forward and reverse genetics screens.

The virtues of Petunia as a model system have only partly been highlighted. In a first monograph, edited by K. Sink and published in 1984, the emphasis was mainly on taxonomy, morphology, classical and biochemical genetics, cytogenetics, physiology and a number of topical subjects. At that time, little molecular data was available. Taking into account that that first monograph will be offered electronically as a supplement in this upcoming edition, we would like to put the overall emphasis for the second edition on molecular developments and on comparative issues.

To this end we propose the underneath set up, where chapters will be brief and topical. Each chapter will present the historical setting of its subject, the comparison with other systems (if available) and the unique progress as made in Petunia. We expect that the second edition of the Petunia monograph will draw a broad readership both in academia and industry and hope that it will contribute to a further expansion in research on this wonderful Solanaceae."

This book is open access under a CC BY 4.0 license. By 2050, human population is expected to reach 9.7 billion. The demand for increased food production needs to be met from ever reducing resources of land, water and other environmental constraints. Rice remains the staple food source for a majority of the global populations, but especially in Asia where ninety percent of rice is grown and consumed. Climate change continues to impose abiotic and biotic stresses that curtail rice quality and yields. Researchers have been challenged to provide innovative solutions to maintain, or even increase, rice production. Amongst them, the 'green super rice' breeding strategy has been successful for leading the development and release of multiple abiotic and biotic stress tolerant rice varieties. Recent advances in plant molecular biology and biotechnologies have led to the identification of stress responsive genes and signaling pathways, which open up new paradigms to augment rice productivity. Accordingly, transcription factors, protein kinases and enzymes for generating protective metabolites and proteins all contribute to an intricate network of events that guard and maintain cellular integrity. In addition, various quantitative trait loci associated with elevated stress tolerance have been cloned, resulting in the detection of novel genes for biotic and abiotic stress resistance. Mechanistic understanding of the genetic basis of traits, such as N and P use, is allowing rice researchers to engineer nutrient-efficient rice varieties, which would result in higher yields with lower inputs. Likewise, the research in micronutrients biosynthesis opens doors to genetic engineering of metabolic pathways to enhance micronutrients production. With third generation sequencing techniques on the horizon, exciting progress can be expected to vastly improve molecular markers for gene-trait associations forecast with increasing accuracy. This book emphasizes on the areas of rice science that attempt to overcome the foremost limitations in rice production. Our intention is to highlight research advances in the fields of physiology, molecular breeding and genetics, with a special focus on increasing productivity, improving biotic and abiotic stress tolerance and nutritional quality of rice.

Advances in molecular biology and genome research in the form of molecular breeding and genetic engineering put forward innovative prospects for improving productivity of many pulses crops. Pathways have been discovered, which include regulatory elements that modulate stress responses (e.g., transcription factors and protein kinases) and functional genes, which guard the cells (e.g., enzymes for generating protective metabolites and proteins). In addition, numerous quantitative trait loci (QTLs) associated with elevated stress tolerance have been cloned, resulting in the detection of critical genes for stress tolerance. Together these networks can be used to enhance stress tolerance in pulses. This book summarizes recent advances in pulse research for increasing productivity, improving biotic and abiotic stress tolerance, and enhancing nutritional quality.

Cash crops are grown and sold for monetary gain and not necessarily for sustenance. They include coffee, tea, coconut, cotton, jute, groundnut, castor, linseed, cocoa, rubber, cassava, soybean, sweet potato, potato, wheat, corn and teff. While some of these crops have been improved for realizing yield potential, breeding of many of them is still in infancy. Crops that underwent rigorous breeding have eventually lost much of the diversity due to extensive cultivation with a few improved varieties and the diversity in less bred species is to be conserved. Over the past years, scholars and policy makers have become increasingly aware of the short and long-run impact of climatic factors on economic, food security, social and political outcomes . Genetic diversity, natural and induced, is much needed for the future generations to sustain food production with more climate resilient crops. In contrast, crop uniformity produced across the farm fields in the form of improved varieties is genetically vulnerable to biotic and abiotic stresses. Thus, it is essential and challenging to address the issue of compromising between maximizing crop yield under a given set of conditions and minimizing the risk of crop failure when conditions change. Cash crops are grown in an array of climatic conditions. Many of the world's poor still live in rural areas. Many are subsistence farmers, operating very small farms using very little agricultural inputs for achieving marketable outputs. Conserving the diversity of these crops and addressing all issues of crop culture through modern tools of biotechnology and genomics is a real challenge. We believe the focus of this book is to fill an unmet need of this and other grower communities by providing the necessary knowledge, albeit indirectly via the academics, to manage the risks of cash crops breeding through managing genetic diversity.

Micropropagation is a reliable technology applied commercially worldwide for large-scale plant multiplication, germplasm conservation, pathogen elimination, genetic manipulations and supply of selected plants. In Protocols for Micropropagation of Selected Economically-Important Horticultural Plants, well recognised researchers in the field compile step-wise protocols for rapid plant multiplication of economically-important horticultural species. The book contains 35 chapters, divided into four major sections. The first three sections (Section A, B and C) contain 29 micropropagation protocols of selected fruit and nut species, indoor and outdoor ornamental plants, cut flowers, and vegetables. In addition to the detailed protocols of in vitro shoot initiation, proliferation, root induction and acclimatization, chapters also include detailed information on medium preparation, explant selection and preparation. The six chapters of Section D cover specific reviews on pivotal topics, such as in vitro rejuvenation, synthetic seed technology, thermotherapy and meristem culture in banana, genetic transformation of pineapple, flower color somaclonal variation in torenia, and cryotherapy of horticultural crops. Moreover, as a part of the highly successful Methods in Molecular Biology series, chapters include introductions to the respective topic, lists of necessary materials, notes, and illustrative photos. Comprehensive and well-written, Protocols for Micropropagation of Selected Economically-Important Horticultural Plants offers a useful resource for horticulturists, researchers, commercial companies, plant propagators, biotechnologists and students interested in micropropagation.

Important breakthroughs have recently been made in our understanding of the cognitive and sensory abilities of pollinators, such as how pollinators perceive, memorize, and react to floral signals and rewards; how they work flowers, move among inflorescences, and transport pollen. These new findings have obvious implications for the evolution of floral display and diversity, but most existing publications are scattered across a wide range of journals in very different research traditions. This book brings together outstanding scholars from many different fields of pollination biology, integrating the work of neuroethologists and evolutionary ecologists to present a multidisciplinary approach.

This book is the first to provide a detailed and critical account of the emergence, development, and implementation of plant variety protection laws in Asian countries. Each chapter undertakes a critical socio-legal analysis of one or more legal frameworks to understand, evaluate, and explore the concerns of diverse national stakeholders, the histories and dynamics of law-making, and the ways in which plant variety protection and seed certification laws interact with local agricultural systems. The book also assesses how Asian countries can capitalise on the 'unused policy space' in international agreements such as the Agreement on Trade-Related Aspects of Intellectual Property Rights and the International Convention for the Protection of New Varieties of Plants, as well as international obligations beyond these, such as those contained in the Convention on Biological Diversity and the Plant Treaty. It also highlights the many ways in which Asian experiences can offer new insights into the relationship between intellectual property and plants, and how relevant laws might be re-imagined in other regions, including Africa, Europe, and the Americas. By adding an important new perspective to the ongoing debate on intellectual property and plants, this book will appeal to academics, practitioners, and policy-makers engaged in work surrounding intellectual property laws, agricultural biodiversity, and plant breeding.

Cereal Genomics published in 2004 served the purpose of collecting all information on cereal genomics at one place and was well received by the cereal workers through-out the world. The last eight years have witnessed significant advancement in the field of cereal genomics. For instance, high-density genetic maps, physical maps, QTL maps and even draft genome sequence have become available for several cereal species. Furthermore, the next generation sequencing (NGS) technologies have revolutionized genomics research, so that it is possible now to sequence genomes of hundreds or thousands of accessions of an individual cereal crop. Significant amounts of data generated using these NGS technologies created a demand for computational tools to analyse this massive data. In view of these developments, the Editors realised that there was a need to have an updated volume on the present status and future prospects of cereal genomics. These developments related to technology and the tools have been documented in this volume, thus supplementing our earlier edited volume Cereal Genomics . Cereal Genomics II discusses advances in cereal genomics research made during the last eight years, and presents state-of-art cereal genomics and its utilization involving both basic research such as comparative genomics and functional genomics, and applied research like QTL mapping and molecular breeding.

This volume examines the interrelated fields of food security, energy security and sustainable agriculture as the key to a stable global agricultural platform and is arranged in six parts. The first part is focused on policy considerations relating to food and energy security and sustainable agriculture. The authors from this part include Former Under Secretary of Agriculture Gale Buchanan, Former Under Secretary of Energy Raymond Orbach (Chapter 1), Stephen Hughes, Bryan Moser and William Gibbons (Chapter 2) and Thomas Redick (Chapter 3). Part II addresses soil and water, which are two of the key components in secure and sustainable food production. Authors from this part are Jerry Hatfield (Chapter 4) and Mahbub Alam, Sharon Megdal et al. (Chapter 5). The third part covers sustainable and secure food production specifically addressing genetically modified traits in Chapter 6 (James McWilliams) and omega-3 fatty acids in Chapter 7 (Jay Whelan et al.). Agronomic implications relative to food security and sustainable agriculture are described in Part IV. Authors include Ravi Sripada, Pradip Das et al. (Chapter 8), Duska Stojsin, Kevin Matson and Richard Leitz (Chapter 9) and S.H. Lee, David Clay and Sharon Clay (Chapter 10). International sustainable agriculture and food security is addressed in Part V with authors Jeff Vitale and John Greenplate (Chapter 11), Julie Borlaug et al. (Chapter 12) and Sylvester Oikeh et al. (Chapter 13). The final part covers the use of chemicals in sustainable agriculture and food/energy security with Leonard Gianessi and Ashley Williams communicating the role of herbicides and Harold Reetz emphasizing the importance of fertilizers both in maximizing crop yields to maintain a sustainable secure source for food production."

This specially curated collection features four reviews of current and key research on improving crop nutrient use efficiency. The first chapter explores the relationship between rhizobacteria and plant roots, looking primarily at the recruitment of rhizobacteria by the plant to carry out particular functions, such as nutrient acquisition. The chapter highlights our current understanding of the molecular determinants of legume nodulation as well as challenges for improvements of biological nitrogen fixation in legumes and non-legumes. The second chapter considers the rising use of nitrogen (N) fertilizer in agriculture and its role in the shrinking contribution of soil organic N. The chapter explores the impact of the inefficient management of N (low nitrogen-use efficiency) and the consequent developments of major environmental issues, such as pollution to groundwater, oceans and the atmosphere. The third chapter addresses key issues in using N fertilizers in wheat production, such as product cost and environmental impact. The chapter summarises the development of N-efficient cultivars and their economic benefits, as well as their role in reducing the environmental impact of excessive N fertilizer inputs, whilst maintaining respectable yields. The final chapter considers the use of breeding techniques, including genetic variability, to develop more efficient wheat varieties with improved traits related to nitrogen capture, nitrogen assimilation and nitrogen remobilization.

Palms are monocots, Angiosperms, belonging to the family Palmae (Arecaceae), perennials having woody stems. Palmae (Arecaceae) family comprised of about six subfamilies, 200 genera and 2,700 species that are distributed all over the tropical, subtropical and Mediterranean landscape. Palms are diverse (ecologically and morphologically) group of plants. Ornamental palms are important component of landscape as well as interiorscapes. Additionally, these plants are good source of food, feed and shelter with numerous other commercial benefits. Likewise other trees and crops, landscape and field nurseries of palms are also subjected to various threats of insect pest and diseases (caused by different plant pathogens). Amongst fungal diseases leaf spots, leaf blights, Fusarium wilts, butt rots, bud rots, root rots, lethal yellowing and decline of palms are major growth constraints of palm growth. In developing countries very little attention has been paid on the etiology and management of these fungal diseases on ornamental palms. Accurate diagnosis and reliable management plan of palm fungal diseases usually requires expertise in both modern and advanced plant pathological approaches. Historically it was general belief that plant pathogens are not associated with human diseases. Since 19th century, several clinical reports are available indicating many plant pathogenic fungi (Aspergillus spp., Penicillium spp., Alternaria spp., Trichoderma spp., Fusarium spp., Curvularia spp. and Colletotrichum Spp) as novel agents of human diseases. Besides the association of fungal plant pathogens infecting ornamental palms, harbouring any of earlier mentioned or other fungal species (capable of causing certain diseases in human beings or pets) by the ornamental palms cultivation (either grown indoor or outdoor) is an important area of research to be explored and addressed thoroughly. This book will provide the deep information regarding major fungal diseases of ornamental palms, their symptoms, disease identification, and etiology and management strategies. This book will also provide unique knowledge regarding the ornamental palms harbouring kinds of human fungal pathogens and their practical management at domestic and commercial scale, in order to make cultivation of these plant more beneficial for humans, animals and environment.

Genetic engineering and biotechnology along with conventional breeding have played an important role in developing superior cultivars by transferring economically important traits from distant, wild and even unrelated species to the cultivated varieties which otherwise could not have been possible with conventional breeding. There is a vast amount of literature pertaining to the genetic improvement of crops over last few decades. However, the wonderful results achieved by crop scientists in food legumes' research and development over the years are scattered in different journals of the World. The two volumes in the series 'Alien Gene Transfer in Crop Plants' address this issue and offer a comprehensive reference on the developments made in major food crops of the world. These volumes aim at bringing the contributions from globally renowned scientists at one platform in a reader-friendly manner. The 1st volume entitled, 'Alien Gene Transfer in Crop Plants: Innovations, Methods and Risk Assessment" will deal exclusively with the process and methodology. The contents of this volume have been designed to appraise the readers with all the theoretical and practical aspects of wide hybridization and gene transfer like processes and methods of gene transfer, role of biotechnology with special reference to embryo rescue, genetic transformation, protoplast fusion and molecular marker technology, problems such as cross incompatibility and barriers to distant hybridization and solutions to overcome them. Since wild and weedy relatives of crop plants may have negative traits associated with them, there are always possibilities of linkage drag while transferring alien alleles. Therefore, problems and limitations of alien gene transfer from these species will also be discussed in this series. Further, the associated risks with this and assessment of risks will also be given due weightage.

This book presents up-to-date information on various vector-less/direct (physical, chemical) and vector-mediated/indirect (Agrobacterium-mediated) plant transformation techniques. It summarizes various strategies that facilitate a gene from lower organism to be expressed in higher plants and also in silico designing of synthetic gene for higher expression. It also highlights the importance of strong promoters to drive the expression of transgene(s). This book encompasses the advantages and drawbacks of cisgenesis and transgenesis, their implications towards sustainable crop improvement, and their future prospects. The importance, limitations, challenges, recent developments, and future prospects of molecular pharming is also discussed. The book concludes with a chapter that summarizes the major contribution of GM-crops towards global food security and economy, advances in genome editing for crop improvement, challenges and risk associated with the release of GM-crops, and the future of GM technology. This book is meant for students and researchers in the field of life sciences, food science, and agriculture.

Increasing agro productivity to feed a growing global population under the present climate scenario requires optimizing the use of resources and adopting sustainable agricultural production. This can be achieved by using plant beneficial bacteria, i.e., those bacteria that enhance plant growth under abiotic stress conditions, and more specifically, microorganisms such as plant growth promoting rhizobacteria (PGPR), which are the most promising candidates in this regard. Attaining sustainable agricultural production while preserving environmental quality, agro-ecosystem functions and biodiversity represents a major challenge for current agricultural practices; further, the traditional use of chemical inputs (fertilizers, pesticides, nutrients etc.) poses serious threats to crop productivity, soil fertility and the nutritional value of farm produce. Given these risks, managing pests and diseases, maintaining agro-ecosystem health, and avoiding health issues for humans and animals have now become key priorities. The use of PGPR as biofertilizers, plant growth promoters, biopesticides, and soil and plant health managers has attracted considerable attention among researchers, agriculturists, farmers, policymakers and consumers alike. Using PGPR can help meet the expected demand for global agricultural productivity to feed the world's booming population, which is predicted to reach roughly 9 billion by 2050. However, to do so, PGPR strains must be safe for the environment, offer considerable plant growth promotion and biocontrol potential, be compatible with useful soil rhizobacteria, and be able to withstand various biotic and abiotic stresses. Accordingly, the book also highlights the need for better strains of PGPR to complement increasing agro-productivity.

While there has been great progress in the development of plant breeding over the last decade, the selection of suitable plants for human consumption began over 13,000 years ago. Since the Neolithic era, the cultivation of plants has progressed in Asia Minor, Asia, Europe, and ancient America, each specific to the locally wild plants as well as the ecological and social conditions. A handy reference for knowing our past, understanding the present, and creating the future, this book provides a comprehensive treatment of the development of crop improvement methods over the centuries. It features an extensive historical treatment of development, including influential individuals in the field, plant cultivation in various regions, techniques used in the Old World, and cropping in ancient America. The advances of scientific plant breeding in the twentieth century is extensively explored, including efficient selection methods, hybrid breeding, induced polyploidy, mutation research, biotechnology, and genetic manipulation. Finally, this book presents information on approaches to the sustainability of breeding and to cope with climatic changes as well as the growing world population.

One of the predicted consequences of the depletion of stratospheric ozone is an increase in the amount of ultraviolet light reaching the surface of the earth, in particular UV-B (320-280nm). Although the real effects are as yet unknown, this change in radiation could have profound consequences for plant growth and productivity. The need for information concerning the relationship between plants and UV-B is therefore pressing. This volume brings together authoritative contributions from leading experts in UV-B/plant studies and is unique in considering interactions at various scales, ranging from the level of the cell through to the level of the community. Information concerning ozone depletion and physical aspects of UV-B radiation complements the biological information to provide a thorough and comprehensive review of the status of knowledge.