Proceedings of a Seminar in the CEC Programme of Research on Plant Protein Improvement, held in Copenhagen 11th-13th September 1984

In the series of International Protoplast Symposia the Symposium of 1987 was held in Wageningen (The Netherlands). Earlier Symposia took place in Jena (DDR) 1963, Brno (CSSR) 1967, Salamanca (Spain) 1971, Nottingham (UK) 1975, Szeged (Hungary) 1979 and Basel (Switzerland) 1983. This 7th International Protoplast Symposium was organized by K.J. Puite (Secretary), J.J.M. Dons (Treasurer), H.J.Huizing and E.J.L. Hotke-Staal (Local Organizers), the first three persons being scientists, respectively, from the Research Institute Ital, the Institute for horticultural plant breeding IVT and the Foundation for agricultural plant breeding SVP at Wageningen. Scientific Advisers of the Symposium were A. J. Kool, M. Koornneef and F.A. Krens. The International Agricultural Centre lAC served as the Symposium location. The Organizing Committee decided that the scientific programme of the Symposium should be mainly focussed on protoplast technology of relevance to plant breeding. Therefore research on microbial protoplasts and on secondary metabolites was not included. About 250 scientists from 27 different countries were welcomed at the meeting. Speakers at Symposium Sessions and authors of Poster contributions were asked to hand over their manuscripts for the Symposium Proceedings already at the meeting, permit ting early publication of the Proceedings. These manuscripts give the state of the art of the protoplast research and illustrate the progress since the last Protoplast Symposium.

Part of a series which presents papers of topical interest relating to the breeding of plants important to agriculture and horticulture.

The field of plant breeding has grown rapidly in the last decade with breakthrough research in genetics and genomics, inbred development, population improvement, hybrids, clones, self-pollinated crops, polyploidy, transgenic breeding and more. This book discusses the latest developments in all these areas but explores the next generation of needs and discoveries including omics beyond genomics, cultivar seeds and intellectual and property rights. This book is a leading-edge publication of the latest results and forecasts important areas of future needs and applications.

This open access book focuses on the linear selection index (LSI) theory and its statistical properties. It addresses the single-stage LSI theory by assuming that economic weights are fixed and known - or fixed, but unknown - to predict the net genetic merit in the phenotypic, marker and genomic context. Further, it shows how to combine the LSI theory with the independent culling method to develop the multistage selection index theory. The final two chapters present simulation results and SAS and R codes, respectively, to estimate the parameters and make selections using some of the LSIs described. It is essential reading for plant quantitative geneticists, but is also a valuable resource for animal breeders.

More than 20 million childhood deaths occur every year due to the micronutrient deficiency and diet-related non-communicable diseases (cardiovascular diseases, cancers, chronic respiratory diseases and diabetes). The United Nations (UN) recently announced that the increase in chronic, non-communicable diseases has resulted in 36 million deaths around the world annually, claiming more lives than all other causes combined. These chronic diseases are not isolated to developed countries and are even more pronounced in the developing world. Such chronic illnesses have caused far more deaths than infectious diseases throughout the world (except Africa) in recent years. Therefore, enrichment of micronutrients in staple food crops is of paramount importance for the nutritional security in our world. Biofortification is the development of micronutrient- and/or vitamin-rich crops using traditional crop improvement practices as well as modern biotechnology tools. It is a more sustainable and cost effective method than food supplementation, fortification and diet diversification. This work consolidates available information on the different aspects of breeding for improved nutrition of pulses. An overview of entire pulses based on their nutritional profile is given so that audience can find the desired information easily. Food legumes are the active ingredients in many gluten-free food products and there is a continuous rise of the use of pulses flour in milling and baking processes. Our book sheds light on recent efforts and the underlying constraints of meeting the public demand. We believe this work provides the basic information for anyone interested in biofortification and stimulate further research to meet this unique challenge.

Originating in South America, cassava is grown in over 100 countries around the world. It is the third most important source of calories in the tropics after rice and maize. Its caloric value, as well as its ability to tolerate dry conditions and poor soils, makes it a key food security crop in developing countries. As demand for food grows, there is an urgent need to increase yields in the face of such challenges as climate change, threats from pests and diseases and the need to make cultivation more resource-efficient and sustainable. Drawing on an international range of expertise, this collection focuses on ways of improving the cultivation of cassava at each step in the value chain, from breeding to post-harvest storage. Volume 2 starts by reviewing genetic resources, advances in breeding and their application to produce varieties with desirable traits such as higher yield. It then goes on to review developments in understanding and managing pests and diseases. Achieving sustainable cultivation of cassava Volume 2: Genetic resources, breeding, pests and diseases will be a standard reference for agricultural scientists in universities, government and other research centres and companies involved in improving cassava cultivation. It is accompanied by Volume 1 which reviews cultivation techniques.

"These books present a comprehensive coverage of issues facing wheat production globally. The authors represent the top scientists involved in the diverse areas that are important for sustainable wheat production and will this book provides an excellent resource for those interested in wheat improvement and production." Dr Hans-Joachim Braun, Director Global Wheat Program and CRP Wheat, International Maize and Wheat Improvement Center (CIMMYT), Mexico Wheat is the most widely cultivated cereal in the world and a staple food for around 3 billion people. It has been estimated that demand for wheat could increase by up to 60% by 2050. There is an urgent need to increase yields in the face of such challenges as climate change, threats from pests and diseases and the need to make cultivation more resource-efficient and sustainable. Drawing on an international range of expertise, this collection focuses on ways of improving the cultivation of wheat at each step in the value chain, from breeding to post-harvest storage. Volume 1 reviews research in wheat breeding and quality traits as well as diseases and pests and their management. Chapters in Part 1 review advances in understanding of wheat physiology and genetics and how this has informed developments in breeding, including developing varieties with desirable traits such as drought tolerance. Part 2 discusses aspects of nutritional and processing quality. Chapters in Part 3 cover research on key wheat diseases and their control as well as the management of insect pests and weeds. Achieving sustainable cultivation of wheat Volume 1: Breeding, quality traits, pests and diseases will be a standard reference for cereal scientists in universities, government and other research centres and companies involved in wheat cultivation. It is accompanied by Volume 2 which reviews improvements in cultivation techniques.

This work presents state-of-the-art overviews on topics relating to the breeding of agriculturally and horticulturally important plants. It is a series that appears in the form of one or more hardbound volumes per year. The text monitors developments in plant breeding research and covers major field crops, horticultural crops and specialities such as mushrooms and nuts. It also includes trees grown for timber and pulp.

This book provides up-to-date information on the environmental impact of transgenic trees on genetically modified tree (GMT) communication strategy. It is useful to public/private organisations as well as to private and public research bodies and universities worldwide since it reports on the global status of GMT research and policy. A high number of genetically modified trees (GMTs) with altered or novel characteristics have been produced in the last 15 years. However, their very low public acceptance is a basic problem in their commercialization. Breeders anticipate economic and ecological benefits, like reduced product costs and less pressure on native forests, while opponents fear risks, such as unintended spread of GMTs. But what is true? To answer this question, the COST Action FP0905 focused on key aspects related to GMTs: (a) biological characterization; (b) assessment of possible environmental impacts; (c) socio-economic implications and public acceptance/concerns; (d) providing science-based information to communicate with the public.

Plant Breeding Reviews is an ongoing series presenting state-of-the art review articles on research in plant genetics, especially the breeding of commercially important crops. Articles perform the valuable function of collecting, comparing, and contrasting the primary journal literature in order to form an overview of the topic. This detailed analysis bridges the gap between the specialized researcher and the broader community of plant scientists.

Rice is one of the most important foods in the world. As the demand for rice continues to increase, there is an urgent need to increase yields in the face of such challenges as climate change, threats from pests and diseases and the need to make cultivation more resource-efficient and sustainable. Drawing on an international range of expertise, this collection focuses on ways of improving the cultivation of rice at each step in the value chain, from breeding to post-harvest storage. Volume 1 reviews research in physiology and breeding and its application to produce varieties with improved traits such as higher yields. It then goes on to discuss nutritional and other aspects of rice quality and the ways these can be enhanced. Achieving sustainable cultivation of rice Volume 1: Breeding for higher quality and yield will be a standard reference for rice scientists in universities, government and other research centres and companies involved in rice cultivation. It is accompanied by Volume 2 which reviews improvements in cultivation techniques, pest and disease management.

Mushrooms are fleshy fungi with a high prospective for the production of secondary metabolites including extracellular enzymes with high agricultural and biotechnological significance. Worldwide, they are well recognized as supplementary foods due to their high nutritional values and their medicinal importance, which includes their uses in exhibiting antioxidant and antimicrobial activities, immune enhancer, and to be effective for the treatment of several diseases including diabetes and few types of cancers as well. According to recent studies, extracellular enzymes produced by several white-rot fungal strains such as Phanerochaete chrysosporium, Pleurotus sajor-caju and several mushrooms have shown a high capacity to decolorize dyes that are very harmful for the environment. Moreover, wild macrofungi have the capability to synthesize nanoparticles which are more useful for the treatment of cancer, gene therapy, DNA analysis and biosensors. Wild macrofungi are extremely important model for basic biology and commercial manufacture.

This open access book shares the experiences of Tropical Legumes III (TLIII) project in facilitating access to seed of improved legume varieties to smallholder farmers through innovation platforms. It highlights practices and guiding principles implemented in eight developing countries of sub-Saharan Africa and South Asia. This book details key processes that respective teams employed to create an innovation space that delivers seed, other inputs, knowledge and financial services to agricultural communities and most importantly, the underserved farmers in remote areas of the drylands. It offers valuable insights into the pathway to establishing, promoting and operating innovation platforms to enhance the performance and competitiveness of legume crops' value chains, and addresses critical issues that must be considered to make innovation platforms more sustainable and attractive to beneficiaries. The book offers a wealth of practical insights for development workers, technical staff, and project managers. This publication is all about TLIII community of practice. It will definitely inspire other development workers and scientists to share their own experiences for others to learn from.

A range of techniques is available to the plant breeder today to complement classical breeding methods. The options are based on the integration of advances in plant cell biology with those in plant molecular biology. Plant cell, tissue and organ cultures provide efficient systems for transformation, for the achievement of wide crosses and for the production of variation through spontaneous and induced mutation, while permitting effective isolation of desired genotypes by in vitro selection. This volume presents a critical appraisal of the methodologies of plant genetic manipulation for advanced undergraduates, postgraduates, researchers and plant breeders, and provides guidance on the choice of breeding options. The latter depends on the breeding system of the crop, the breeding objective and the tissue culture systems applicable to the target genotype(s).

This book includes papers from keynote lecture and oral presentations of Plant and Microbe Adaptations to Cold (PMAC) 2012, an international conference on winter hardiness of crop and pathogenic microbes. The PMAC has been started in 1997 in Japan as an interdisciplinary forum for scientists and extension people working in the field in plant pathology, plant physiology, microbiology, and crop breeding to increase our knowledge and improve our understanding of overwintering of crops, forages and grasses and solve the problems associated with losses due to freezing and heavy snow cover. Successive meetings have been held in Iceland (2000), Canada (2003), Italy (2006), and Norway (2009). PMAC2012 will be a special meeting with a focus on global climate change, food security and agriculture sustainability and the whole program will be arranged to reflect this theme. The topics covered by this proceedings includes, global warming in agricultural environment, plant adaptations to cold, microbial adaptations to cold, plant-microbe interaction under cold, and molecular breeding for winter hardiness. The researches range from molecular biology to ecology and breeding. Experts in the field will report cutting edge research and thoughtful strategies for sustainability.

Plant breeding has played a significant role in the development of human civilizations. Conventional plant breeding has significantly improved crop yield by genetically manipulating agronomically important traits. However, it has often been criticized for ignoring indigenous germplasm, failing to address the needs of the marginal and the poor farmers, and emphasizing selection for broad instead of local adaptation. Participatory plant breeding (PPB) is the process by which the producers and other stakeholders are actively involved in a plant-breeding programme, with opportunities to make decisions throughout. The Working Group on Participatory Plant Breeding (PPBwg) was established in 1996 under the framework of the Consultative Group on International Agricultural Research (CGIAR). Research in PPB can promote informed participation and trust in research among consumers and producers, and in recent years, PPB has had a significant impact on food production by quickly and cost-effectively producing improved crop varieties. At the same time, there has been significant research in the area. PPB offers significant advantages that are particularly relevant to developing countries where large investments in plant breeding have not led to increased production, especially in the marginal environments. In addition to the economic benefits, participatory research has a number of psychological, moral, and ethical benefits, which are the consequence of a progressive empowerment of the farming communities. PPB can empower groups such as women or less well-off farmers that are traditionally left out of the development process. This book explores the potential of PPB in the coming decades. The topic is more relevant since international breeding efforts for major crops are aimed at decentralizing local breeding methods to better incorporate the perspective of end users into the varietal development process. The first book incorporating the upcoming research on this novel breeding approach, it reviews the important tools and applications of PPB in an easy-to-read, succinct format, with illustrations to clarify these complex topics. It provides readers with a basic idea of participatory plant breeding as well as advances in the field and insights into the future to facilitate the successful integration of farmers into breeding programmes. This book is a valuable reference resource for agriculturists, agricultural advisers, policy makers, NGOs, post-doctoral students and scientists in agriculture, horticulture, forestry and botany.

Plant adaptation is a fundamental process in plant breeding. It was the first criterion in the initial domestication of plants thousands of years ago. Adaptedness is generally a quantitative complex feature of the plant, involving many traits, many of which are quantitative. Adaptation to stresses like cold, drought or diseases are among the most central problems in a world grappling with global food security. Modern plant breeding, based on mendelian genetics, has made plant improvement more effective and more precise and selective. Molecular genetics and genetic engineering has considerably increased this selectivity down to single genes affecting single traits. The time has come when plant breeding efficiency may cause loss of genetic resources and adaptation. In these proceedings an effort is made to merge modern plant breeding efficiency with ecological aspects of plant breeding, reflected in adaptation. It is hoped that this merger results in more sustainable use of genetic resources and physical environments. The book is based on 10 keynotes addressing a wide spectrum of themes related to adaptation. In addition each subject is further elaborated in up to three case studies on particular plant species or groups of plants. The keynotes do in fact overlap to some degree and there are articles in this volume that seemingly contradict each other, a common aspect in advanced fields of research. The keen reader may conclude that, in a world where climates and environments are under continuous change and where human society is more and more polarized into a developed and a developing part, adaptation of our cultivated plants has different constraints on yields depending on ecology, and indeed economy.

This book reviews modern strategies in the breeding of vegetables in the era of global warming. Agriculture is facing numerous challenges in the 21st century, as it has to address food, nutritional, energy and environmental security. Future vegetable varieties must be adaptive to the varying scenarios of climate change, produce higher yields of high- quality food and feed and have multiple uses. To achieve these goals, it is imperative to employ modern tools of molecular breeding, genetic engineering and genomics for 'precise' plant breeding to produce 'designed' vegetable varieties adaptive to climate change. This book is of interest to scientists working in the fields of plant genetics, genomics, breeding, biotechnology, and in the disciplines of agronomy and horticulture.

This collection of essays looks at sexuality and reproduction from an evolutionary perspective. Covering experimental discoveries as well as theoretical investigations, the volume explores the relationship between evolution and other areas of human behaviour.

This book presents recent research on natural fibers extracted from pineapple leaves. Covering several extraction processes, properties of pineapple leaf fibers and comparisons with other natural fibers, and their applications, it provides up-to-date information on the subject of natural fibers from prominent researchers in academia and industry as well as government/private research laboratories across the world. The book is a comprehensive reference resource for university and college faculties, professionals, postdoctoral research fellows, undergraduate/graduate students, researchers and scientists working in the areas of non-forest product utilization, natural fibers, and biomass materials.

Asiatic Momordica comprises 10 species of which 3 are monoecious and rest dioecious. They are unique in the sense that all are wild or cultivated vegetables with medicinal uses. Barring bitter gourd, all are under utilized vegetables with little research and conservation efforts. In this book, the authors have presented the available information on various aspects of Asiatic Momordica like taxonomy, cytogenetics, crop improvement, origin, ethnobotany and crop production at one place. The book is well illustrated with color maps and photographs depicting habit, morphology and distribution of individual taxa. Species distribution map for all Indian taxa, descriptor for characterization and preliminary evaluation of dioecious and monoecious group available varieties, keys for botanical identification are some of the unique features of the book. The book will be useful to botanists, horticulturists, foresters, environment educators, conservation biologists, plant science students in general and all interested in agrobiodiversity.

This book collates the most up to date information on Fragaria, and Rubus genomes. It focuses on the latest advances in the model system Fragaria vesca, along with the allied advances in economically important crops. Covering both basic and applied aspects of crop genomics, it illustrates strategies and resources for the study and utilization of genome sequences and aligned functional genomics resources. Rosaceous berries are collectively an increasingly important set of high-value global crops, with a trade value of over GBP2 billion dollars per annum. The rosaceous berries strawberry, raspberry and blackberry share some common features at the genome scale, namely a range of ploidy levels in each genus and high levels of heterozygosity (and associated inbreeding depression) due to self-incompatibility systems, dioecy, or multispecies hybridization events. Taken together, although the genomes are relatively compact, these biological features lead to significant challenges in the assembly and analysis of berry genomes, which until very recently have hampered the progress of genome-level studies. The genome of the woodland strawberry, Fragaria vesca, a self-compatible species with a homozygous genome was first sequenced in 2011 and has served as a foundation for most genomics work in Fragaria and to some extent Rubus. Since that time, building upon this resource, there have been significant advances in the development of genome sequences for related crop species. This, coupled with the revolution in affordable sequencing technology, has led to a suite of genomics studies on Fragaria and more recently Rubus, which undoubtedly aid crop breeding and production in future years.

This work synthesizes research and practical work, including various techniques and applications of botany and nanoparticles, including enzymology, pharmaceuticals, phenolics, antioxidants, metal particles, synthesis of nanoparticles by plants and microbes, and more. The text discusses the latest research as well as key sources of information condensed from other scholars across the globe, providing a comprehensive resource for scholars working in nanobotany, as well as chemists and researchers in the pharmaceutical industry.