This book provides comprehensive information on the latest tools and techniques of molecular genetics and their applications in crop improvement. It thoroughly discusses advanced techniques used in molecular markers, QTL mapping, marker-assisted breeding, and molecular cytogenetics.

Plant tissue culture and advanced biotechnologies have proven to be influential tools that complement conventional breeding and accelerate development of many medicinal plants. Various approaches, such as pathway engineering, precursor feeding, transformation, elicitation with biotic and abiotic elicitors and scaling up in bioreactors, have been explored to improve the production of secondary metabolites from different medicinal plants. This book provides a comprehensive description of various studies, carried out on in vitro culture and hairy root cultures of Catharanthus roseus, Silybum marianum and Digitalis species which have been considered as alternative sources for the production of anti-tumour compounds, flavonolignans and cardenolides. Specific focus is on elicitation strategy for increasing production of bioactive compounds of C. roseus L., S. marianum and Digitalis species to overcome the constrains of conventional propagation. This book is valuable for researchers or students working on medicinal plants, phytochemistry, and plant tissue culture. It also serves as a reference for the pharmaceutical industry.

Written by the leading experts in the field, this book examines the evolutionary advantages of gender dimorphism - the occurence of two sexual forms - and sexual dimorphism - dimorphism in secondary sex characters - in flowering plants. Gender dimorphism is found in approximately 10% of flowering plant species and has been an active topic of research. By contrast, sexual dimorphism in plants is much less widely appreciated. The book consists of three sections: The first introduces readers to the tremendous variety of breeding systems and their evolution in plants and sets the stage for a consideration of the evolution of dimorphism in reproductive and non-reproductive characters. The second section deals with the evolution of secondary sexual characters, including the theory related to the evolution of sexual dimorphism and its empirical patterns. The last section deals with the genetics of gender expression and of secondary sexual characters.

Written for plant breeders, researchers and post-graduate students, this book provides a comprehensive review of the methods and underlying theoretical foundations used for selection in plant breeding programmes. The authors review basic elements of population and quantitative genetic theory, and consider in a unique way the tackling of the problems presented by soil heterogeneity and intergenotypic competition when selecting quantitative characters. The efficacy of different methods with regard to developing new varieties of crops are discussed for different modes of reproduction. This work provides a sound basis for informed decision making within the process of a selection programme.

The future of agriculture strongly depends on our ability to enhance productivity without sacrificing long-term production potential. An ecologically and economically sustainable strategy is the application of microorganisms, such as the diverse bacterial species of plant growth promoting bacteria (PGPB). The use of these bio-resources for the enhancement of crop productivity is gaining worldwide importance.

"Bacteria in Agrobiology: Crop Ecosystems" describes the beneficial role of plant growth promoting bacteria with special emphasis on oil yielding crops, cereals, fruits and vegetables. Chapters present studies on various aspects of bacteria-plant interactions, soil-borne and seed-borne diseases associated with food crops such as rice, sesame, peanuts, and horticultural crops. Further reviews describe technologies to produce inoculants, the biocontrol of post harvest pathogens as a suitable alternative to agrochemicals, and the restoration of degraded soils.

This book - the first published on this topic in plants - presents the reader with an overview of recent research on nitric oxide (NO) in plants, which, in view of its empirical interest and its growth regulatory potential, is in the forefront of scientific endeavor in plant science. Subject matter is divided into two parts: Part 1 deals with NO and peroxynitrite biochemistry and regulative mechanisms as presently known in the Plant Kingdom and outlines some of the problems still awaiting clarification. Emphasis is placed on ethylene emission regulation, postharvest control, plant phytopathology and environmental stress tolerance. A further topic is plant NO, like Viagra, related to cyclic nucleotide turnover. Part 2 deals with environmental aspects of NO as an atmospheric pollutant and discusses endogenous means which plants at times employ to cope with this particular type of stress, and how their coping mechanisms may be harnessed for purposes of depollution and augmentation of nitrogen fertilization. The text, accompanied by a wealth of illustrations and annotated references, is intended for lecturers, advanced students and research scientists at universities and research institutes dealing with plant sciences and agriculture, as well as for environmental researchers.

This book provides an overview of the Ocimum genus from its genetic diversity to genome sequences, metabolites and their therapeutic utilities. Tulasi, Ocimum tenuiflorum, as a member of the family Lamiaceae, is a sacred plant in India. The plants of this genus Ocimum are collectively referred to as Basil and holy basil is worshipped in the Hindu religion. Basils are reservoirs of diverse terpenoids, phenylpropanoids and flavonoids, in addition to commercially important aromatic essential oils. In 2016, two working groups in India published the genome sequence in two different genotypes of Ocimum tenuiflorum. To help the readers understand the complexities of the genus and different chemotypes, this book accumulates all the available information on this medicinal plant including the genome. The complete knowledge may enable researchers to generate specific chemotypes in basil either through conventional breeding or development of transgenic lines. It also makes it possible to investigate the medicinal nature of holy basil compared to different species of the same genus.

Grassland farming in Europe was already established during the settlement of the rst farmers together with their domesticated animals after the last ice age. Since then, grassland provides the forage basis to feed ruminant animals for the p- duction of meat and milk. Depending on the ecological conditions and intensity of usage, various plant communities with different species developed, displaying a rich biodiversity. With the introduction of improved crop rotations at the end of the 16th century, grasses and legumes were also grown to an important extent as forage crops on arable land. In the last decades the importance of amenity grasses increased markedly, due to the demand of the society for new usages like landscape protection. Around 1900 interested farmers and academics identi ed the need for gra- land improvement through systematic selection and seed production. This marks the beginning of breeding and research in companies but also at universities and specialized research institutes. Plant collection started with many of the species that are still of importance today. The collected materials were grouped according to the intended use and some type of phenotypic selection was applied. Seed mul- plication of such populations was performed in pure stands and the harvested seed was marketed. Although the vegetative biomass and its quality are of utmost imp- tance in forage crop breeding, it is the seed yield potential which determines the commercial success of a new variety.

This book highlights some of the most important biochemical, physiological and molecular aspects of plant stress, together with the latest updates. It is divided into 14 chapters, written by eminent experts from around the globe and highlighting the effects of plant stress (biotic and abiotic) on the photosynthetic apparatus, metabolites, programmed cell death, germination etc. In turn, the role of beneficial elements, glutathione-S-transferase, phosphite and nitric oxide in the adaptive response of plants under stress and as a stimulator of better plant performance is also discussed. A dedicated chapter addresses research advances in connection with Capsicum, a commercially important plant, and stress tolerance, from classical breeding to the recent use of large-scale transcriptome and genome sequencing technologies. The book also explores the significance of the liliputians of the plant kingdom (Bryophytes) as biomonitors/bioindicators, and general and specialized bioinformatics resources that can benefit anyone working in the field of plant stress biology. Given the information compiled here, the book will offer a valuable guide for students and researchers of plant molecular biology and stress physiology alike.

Forage plant breeding has entered the genome era. This timely book reviews the latest advances in the development and application of molecular technologies which supplement conventional breeding efforts for our major forage crops. It describes the plethora of new technologies and tools now available for high-throughput gene discovery, genome-wide gene expression analysis, production of transgenic plants, genome analysis and marker-assisted selection as applied to forage plants. Detailed accounts are presented of current and future opportunities for innovative applications of these molecular tools and technologies in the identification, functional characterisation, and use of valuable genes in forage production systems and beyond. This book represents a valuable resource for plant breeders, geneticists, and molecular biologists, and will be of particular relevance to advanced undergraduates, postgraduates, and researchers with an interest in forage legumes and grasses.

This comprehensive book details the most recent advances in the microalgae biological sciences and engineering technologies for biomass and biofuel production in order to meet the ongoing need for new and affordable sources of food, chemicals and energy for future generations. The chapters explore new microalgae cultivation techniques, including solid (biofilm) systems, and heterotrophic production methods, while also critically investigating topics such as combining wastewater as a source of nutrients, the effect of CO2 on growth, and converting biomass to methane through anaerobic digestion. The book highlights innovative bioproduct optimization and molecular genetic techniques, applications of genomics and metabolomics, and the genetic engineering of microalgae strains targeting biocrude production. The latest developments in microalgae harvesting and dewatering technologies, which combine biomass production with electricity generation, are presented, along with detailed techno-economic modeling. This extensive volume was written by respected experts in their fields and is intended for a wide audience of researchers and engineers.

This volume contains comprehensive reviews on the current status of knowledge concerning the physiological, biochemical, and molecular events underlying plant morphogenesis processes authored by the main lecturers at the NATO Advanced Study Institute which was held in Heraklion, Crete, Greece, Septe

In a presentation to the Linnean Society of London in November 1831, the Scottish botanist Robert Brown (perhaps better known for his discovery of Brownian motion) mentioned almost as an afterthought that in orchid epidermal cells, a single "circular areola" could be seen, a "nucleus of the cell as perhaps it might be termed." Thus, the term "nucleus" (from Latin nucleus or nuculeus, "little nut" or kernel) was born for the compartment of the eukaryotic cell that contains the maj- ity of genetic information. One hundred and seventy-seven years later, we know that the nucleus is the site where genetic information is stored in the form of DNA, and where it is protected from damage, duplicated, divided, recombined, repaired, and "expressed." For the latter, the genetic information is faithfully transcribed from DNA to RNA, then released from the nucleus into the surrounding cytoplasm. Most likely translated into polypeptide chains, the information re-enters the nucleus in the form of diverse proteins that function in the processes listed above.

This book provides in-depth reviews of the effects of nanoparticles on the soil environment, their interactions with plants and also their potential applications as nanofertilizers and pesticides. It offers insights into the current trends and future prospects of nanotechnology, including the benefits and risks and the impact on agriculture and soil ecosystems. Individual chapters explore topics such as nanoparticle biosynthesis, engineered nanomaterials, the use of nanoclays for remediation of polluted sites, nanomaterials in water desalination, their effect on seed germination, plant growth, and nutrient transformations in soil, as well as the use of earthworms as bioremediating agents for nanoparticles. It is a valuable resource for researchers in academia and industry working in the field of agriculture, crop protection, plant sciences, applied microbiology, soil biology and environmental sciences.

This book describes the sequencing efforts for Neem (Azadirachta indica A. Juss), one of the most versatile tropical evergreen tree species. The neem tree is a source of various natural products, including the potent biopesticide azadirachtin and limonoids, which have a broad spectrum of activity against insect pests and microbial pathogens. To identify genes and pathways in neem, three neem genomes and several transcriptomes are studied using next-generation sequencing technologies. Neem has been extensively used in Ayurveda, Unani and homoeopathic medicine and is often referred to as the 'village pharmacy' by natives due to its wealth of medicinal properties. Besides the description of the genome, this book discusses the neem microbiome and its role in the production of neem metabolites like salanin, nimbin and exopy-azadirachtin under in vitro conditions. It also highlights cell and tissue culture using various neem explants including the leaf, root, shoot, cambium, etc.

Red clover (Trifolium pratenseL. ) is an important forage of most ofthe tem- perate regions of the world, and atone time was the most importantlegume for hay in the United States. Oddly enough,red clover has never been the subject ofa monograph, although its culture has been discussed in many monograph chapters in the United States and Europe, perhaps beginning with those of Westgate and Hillman ina USDAFarmers Bulletin in 1911and early Swedish publications. The monograph "CloverScience and Technology" published in 1985 provides coverage of red clover along with other Trifolium species. It was theobjective of thepresentmonograph, "Red Clover Science" toexamine the literature published on red clover primarily after 1984. Perhaps the tim- ing of this publication is appropriate with the present interest in sustainable agriculture ofwhich red clover was so prominent in the past. Red clover and other forage legumes are valuable for reducing our reliance on fossil fuels, and for reducing pollution of our soil, water, and atmosphere primarily from use of chemical nitrogen and other fertilizers. In each chapter, an attempt was made to summarize the earlierinformation and to integrate the recent findings into this background. References earlier than 1984 are included only when necessary for historical purposes. No attempt was made to review the many aspects of clover culture that has been described thoroughly in earlier pub- lications. The literature covered is necessarily worldwide becauseofpresent greater emphasis in northern Eurasia than in United States.

Our requirement for plant breeders to be successful has never been greater. However one views the forecasted numbers for future population growth we will need, in the immediate future, to be feeding, clothing and housing many more people than we do, inadequately, at present. Plant breeding represents the most valuable strategy in increasing our productivity in a way that is sustainable and environmentally sensitive. Plant breeding can rightly be considered as one of the oldest multidisciplinary subjects that is known to humans. It was practised by people who first started to carry out a settled form of agriculture. The art, as it must have been at that stage, was applied without any formal underlying framework, but achieved dramatic results, as witnessed by the forms of cultivated plants we have today. We are now learning how to apply successfully the results of yet imperfect scientific knowledge. This knowledge is, however, rapidly developing, particularly in areas of tissue culture, biotechnology and molecular biology. Plant breeding's inherent multifaceted nature means that alongside obvious subject areas like genetics we also need to consider areas such as: statistics, physiology, plant pathology, entomology, biochemistry, weed science, quality, seed characteristics, repro ductive biology, trial design, selection and computing. It therefore seems apparent that modern plant breeders need to have a grasp of wide range of scientific knowledge and expertise if they are successfully to a exploit the techniques, protocols and strategies which are open to them.

Plant hormones play a crucial role in controlling the way in which plants growand develop. Whilemetabolism providesthepowerand buildingblocks for plant life, it is the hormones that regulate the speed of growth of the individual parts and integrate these parts to produce the form that we recognize as a plant. In addition, theyplayacontrolling role inthe processes of reproduction. This book is a description ofthese natural chemicals: how they are synthesizedand metabolized; howthey work; whatwe knowoftheir molecular biology; how we measure them; and a description ofsome ofthe roles they play in regulating plant growth and development. Emphasis has also been placed on the new findings on plant hormones deriving from the expanding use ofmolecular biology as a tool to understand these fascinating regulatory molecules. Even at the present time, when the role of genes in regulating all aspects of growth and development is considered of prime importance, it is still clear that the path of development is nonetheless very much under hormonal control, either via changes in hormone levels in response to changes in gene transcription, or with the hormones themselves as regulators ofgene transcription. This is not a conference proceedings, but a selected collection ofnewly written, integrated, illustrated reviews describing our knowledge of plant hormones, and the experimental work that is the foundation of this knowledge.

With the predicted increase of the human population and the subsequent need for larger food supplies, root health in crop plants could play a major role in providing sustainable highly productive crops that can cope with global climate changes. While the essentiality of roots and their relation to plant performance is broadly recognized, less is known about their role in plant growth and development. Root Genomics examines how various new genomic technologies are rapidly being applied to the study of roots, including high-throughput sequencing and genotyping, TILLING, transcription factor analysis, comparative genomics, gene discovery and transcriptional profiling, post-transcriptional events regulating microRNAs, proteome profiling and the use of molecular markers such as SSRs, DArTs, and SNPs for QTL analyses and the identification of superior genes/alleles. The book also covers topics such as the molecular breeding of crops in problematic soils and the responses of roots to a variety of stresses.

Plant hormone research is the favorite topic of physiologists. Past three decades have witnessed that this subject has received much attention. The inquisitive nature of human mind has pumped much in literature on this subject and this volume is the product of such minds. In the following pages various hormonal-controlled physiological processes like, flowering, seed dormancy and germination, enzyme secretion, senes cence, ion transport, fruit ripening, root growth and development, thig momorphogenesis and tendril thigmonasty have been included. The volume also contains a review paper on 'Growth Regulating Activity of Penicillin in Higher Plants' and has been presented for the first time. The vast contents of each review paper have been written by erudite scholars who have admirably carried out their evangelic task to make the text up TO date. This volume, I am sure, would stimulate the appetite of researchers of peripheral disciplines of botany and agricultural sciences and they will continue to enjoy the fun and adventures of plant hormone research. Save one. my most outstanding debts are due to the rich array of the contributors and other plant physiologists specially to Prof. Thomas Gaspar (Belgium), Prof. E. E. Goldschmidt (Isreal), Prof. H. Greppin (Switzerland), Dr. K. Gurumurti (India), Prof. M. A. Hall (U. K. ), Prof. H. Harada (Japan), Dr. M. Kaminek (Czechoslovakia), Dr. J. L. Karm oker (BangIa Desh), Prof. Peter B. Kaufman (U. S. A. ), Dr. V. I. Kefeli . / (U. S. S. R. ), Dr. M. Kutaoek (Czechoslovakia), Prof. S."

Since the beginning of agricultural production, there has been a continuous effort to grow more and better quality food to feed ever increasing popula tions. Both improved cultural practices and improved crop plants have al lowed us to divert more human resources to non-agricultural activities while still increasing agricultural production. Malthusian population predictions continue to alarm agricultural researchers, especially plant breeders, to seek new technologies that will continue to allow us to produce more and better food by fewer people on less land. Both improvement of existing cultivars and development of new high-yielding cultivars are common goals for breeders of all crops. In vitro haploid production is among the new technologies that show great promise toward the goal of increasing crop yields by making similar germplasm available for many crops that was used to implement one of the greatest plant breeding success stories of this century, i. e., the development of hybrid maize by crosses of inbred lines. One of the main applications of anther culture has been to produce diploid homozygous pure lines in a single generation, thus saving many generations of backcrossing to reach homozygosity by traditional means or in crops where self-pollination is not possible. Because doubled haploids are equivalent to inbred lines, their value has been appreciated by plant breeders for decades. The search for natural haploids and methods to induce them has been ongoing since the beginning of the 20th century."

This book provides insights into the genetics and the latest advances in genomics research on the common bean, offering a timely overview of topics that are pertinent for future developments in legume genomics. The common bean (Phaseolus vulgaris L.) is the most important grain legume crop for food consumption worldwide, as well as a model for legume research, and the availability of the genome sequence has completely changed the paradigm of the ongoing research on the species. Key topics covered include the numerous genetic and genomic resources, available tools, the identified genes and quantitative trait locus (QTL) identified, and there is a particular emphasis on domestication. It is a valuable resource for students and researchers interested in the genetics and genomics of the common bean and legumes in general.

This book describes the latest advances in Allium genome research. Allium includes plant species known for their huge nuclear genome size, which makes them ideal for somatic chromosome observations in high school experiments. In order to advance the genome analysis of A. cepa and its functional study, scientists in international research collaborations have developed several types of artificially manipulated genetic stocks and analyzed them using modern technologies. The Allium vegetable crop includes garlic, shallot, wakegi onion, Japanese bunching onion, and rakkyo. Bulb onion is one of the world's most important Allium commercial crops, with an estimated annual production of 85.8 million tons in 2013, and ranking third after tomato and watermelon in terms of global vegetable crops.

The present volume presents essential information on advancements in oilseed production, processing and utilization. Advances in the technology of seed processing to produce oil and oil quality for edible and industrial applications are well presented, followed by hybrid technology, biotechnology, oil technology and meal quality for animal nutrition. The following areas are also covered: the potential for oil in developing biodiesel markets, fatty acid long chains and their derivative, pollination management, and safety of pollinators from harmful effects of pesticides. This volume also includes an economic assessment of oilseed integrated pest management (IPM) programs in different regions of the world. Dr. Surinder Kumar Gupta is Professor/Chief Scientist (Oilseeds) Plant Breeding & Genetics and Nodal officer in School of Biotechnology, S K University of Agricultural Sciences & Technology, Faculty of Agriculture, Chatha, Jammu-India. He holds a distinguished academic and service record and has been devoted primarily to research on oilseed Brassicas for nearly two decades. He has written two books on plant breeding and edited three volumes, one on 'Recent Advances in Oilseed Brassicas', Kalyani Publishers, New Delhi, India, second on 'Rapeseed Breeding-Advances in Botanical Research', Vol. 45, Academic Press, Elsevier Publishers and third on Biology and Breeding of crucifers, CRC Publishers, Taylor and Francis Group.

Attention has recently turned to using plants as hosts for the production of commercially important proteins. The twelve case studies in this volume present successful strategies for using plants to produce industrial and pharmaceutical proteins and vaccine antigens. They examine in detail projects that have commercial potential or products that have already been commercialized, illustrating the advantages that plants offer over bacterial, fungal or animal cell-culture hosts. There are many indications that plant protein production marks the beginning of a new paradigm for the commercial production of proteins that, over the next decade, will expand dramatically.