The application of light microscopy to biological problems has made an enormous impact in recent years. This volume provides the key data required to optimize the analysis of biological samples using the light microscope, irrespective of the type of method selected. The Essential Data series provides rapid access to the core data required by researchers on a daily basis, in convenient pocket-sized volumes.

A suitable drug delivery system is an essential element in achieving efficient therapeutic responses of drug molecules. With this desirability in mind, the book unites different techniques through which extremely small-sized particles can be utilized as a successful carrier for curing chronic as well as life-threatening diseased conditions. This is a highly informative and prudently organized book, providing scientific insight for readers with an interest in nanotechnology. Beginning with an overview of nanocarriers, the book impetuses on to explore other essential ways through which these carriers can be employed for drug delivery to varieties of administrative routes. This book discusses the functional and significant features of nanotechnology in terms of Lymphatic and other drug targeting deliveries. The book is presenting depth acquaintance for various vesicular and particulate nano-drug delivery carriers, utilized successfully in Pharmaceutical as well as in Cosmeceutical industries along with brief information on their related toxicities. In addition, the work also explores the potential applications of nanocarriers in biotechnology sciences for the prompt and safe delivery of nucleic acid, protein, and peptide-based drugs. An exclusive section in the book illuminates the prominence and competent applicability of nanotechnology in the treatment of oral cancer. The persistence of this book is to provide basic to advanced information for different novel carriers which are under scale-up consideration for the extensive commercialization. The book also includes recent discoveries and the latest patents of such nanocarriers. The cutting-edge evidence of these nanocarriers available in this book is beneficial to students, research scholars, and fellows for promoting their advanced research.

Dermatopathology is a specialized branch of pathology in which there has been great progress as new techniques have become available to evaluate the pathology of the skin. Many of the advances in our knowledge and understanding of the skin and the diseases that affect it have been made by people with experience of both diagnostic pathology and clinical dermatology. With significant numbers of large textbooks available, there is a need to provide practical clinical information and concise criteria for pathologic diagnosis for pathologists and dermatologists looking for up-to-date diagnostic and management options.

For over thirty years, rodents have been used in the study of many human and animal diseases. The rat in particular continues to serve as an experimental model for drug safety and efficacy tests for the treatment of neurological disorders, heart disease, diabetes, breast, liver and other cancers. With the more recent advent of transgenic technology, an impressive amount of new knowledge about transmissible spongiform encephalopathies has been accumulated. It is likely that the principles learned from the study of these prion diseases will be applicable to elucidating the causes of more common neurodegenerative disorders such as Alzheimer’s and Parkinson’s diseases. As a result of this and, more importantly, because of increasingly complicated legislation governing the use of animals in regulated procedures, there is a need for experimenters (both experienced and those new to the laboratory) to improve their skills continuously and to become au fait with all aspects of preparatory techniques. The Rat Nervous System provides an accessible guide to the dissection and perfusion of rats, placing particular emphasis on the preparation of nervous tissue for further study in the laboratory including biochemical analysis and microscopic study. Logically structured with highly illustrated chapters covering gross anatomy, anatomy of the nervous system, anaesthesia, biological fixation and tissue extraction, the book provides a comprehensive introduction to those techniques required to provide consistent, high-quality tissues for subsequent investigation. Animal husbandry of a high standard, together with minimisation of discomfort, is essential if the information sought by the investigator is to be valid and reproducible; an overview of these topics is therefore also incorporated in this volume.

Make the connections between laboratory and diagnostic testing and the clinical care of your patients! The team that brings you the popular Davis's Comprehensive Handbook of Laboratory and Diagnostic Tests With Nursing Implications now brings you the only text that explains the who, what, when, how, and why of laboratory and diagnostic testing and connects them to clinical presentations, nursing interventions, and nursing outcomes. Using both printed and interactive case studies, you'll see exactly how those connections work in the real-world of clinical practice. Click here for a sneak preview of the text! "As a nursing instructor, the Textbook of Laboratory and Diagnostic Testing: Practical Application of Nursing Process at the Bedside is one book that I would highly recommend be a required textbook for any nursing student. I appreciate that the textbook is distinctly formatted to present diagnostic and laboratory studies while also including case studies. The textbook walks students through understanding why a study is being performed, how it is done, and explains the clinical significance in relation to a given patient situation all while using the nursing process. Absolutely love it!" - Cindy Middaugh, Instructor at El Paso Community College

This book describes some of the key epidemiological principles, scientific approaches and quality assurance frameworks required to design and conduct biobank studies in various settings. Using examples from contemporary biobanks, the book addresses the design features and practical procedures needed in order to launch and manage biobank studies, including consent and regulatory approval, the organisation of field work, management of data and biological samples, follow-up and verification of disease outcomes, development of IT systems for data collection, quality assurance and study management. Over the last two decades, several large biobank studies have been initiated in different populations, intended to greatly enhance the development of precision medicine. Contemporary biobank studies are extremely large and complex, and involve several decades of follow-up. Such studies pose major challenges in terms of ensuring rapid recruitment, obtaining high-quality data, minimising loss to follow-up, reliably classifying disease outcomes, and optimising the use of the biological samples collected. In this regard, the key to success lies not in planning the perfect study, but in planning the most appropriate, reliable, sustainable and future-proof study given the practical constraints of available resources, time and capacity. The authors of this handbook are epidemiologists, clinicians, software engineers, and laboratory and data scientists with extensive experience in conducting large biobank studies. The eight chapters can be read separately or together, and provide readers with essential information on how to design, implement and manage these studies. The state-of-the-art, innovative and scalable approaches and methodologies presented here are intended to stimulate the development of further population-based and hospital-based biobank studies in diverse populations.

This book provides systematic knowledge of basic principles in the design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Fluorescence is the most popular technique in chemical and biological sensing because of its ultimate sensitivity, high temporal and spatial resolution and versatility that enables imaging within the living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response up to detection of single molecules. Its application areas range from control of industrial processes to environment monitoring and clinical diagnostics. Being a guide for students and young researchers, it also addresses professionals involved in active basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.

This book series consists of 3 volumes covering the basic science (Volume 1), clinical science (Volume 2) and the technology and methodology (Volume 3) of autophagy. Volume 3 focuses on the technical aspects of autophagy research. It is comprised of two parts. The first part discusses the basic process of autophagy, including its overall classification and individual stages in the life cycle of autophagosomes. The second part discusses the tools, strategies, and model systems in current autophagy research, including cell and animal models, detection and manipulation methods, as well as screening, genomic, proteomic and bioinformatic approaches. The book is written and edited by a team of active scientists. It is intended as a practical reference resource for interested researchers to get started on autophagy studies.

Over the past 25 years, biobanks of human specimens have become a cornerstone for research on human health and have empowered the "omics "revolution that characterizes biomedical science in the XXIst Century. Today, biobanking of human specimens is a critical component of the interface between clinical practice and translational research, supporting the discovery and validation of new biomarkers of disease etiology, risk, early detection, diagnosis, prognosis, prediction and relapse. With the development of personalized medicine, biobanking of cryopreserved specimens has become standard practice in order to investigate genetic, transcriptomic, proteomic, metabolomics and immunological biomarkers useful to inform caregivers for therapeutic decisions. Data generated from biobanked specimens represent a rapidly growing and highly valuable resource, participating in the emergence of Big Data Medicine. With the development of large computing capabilities and artificial intelligence, data associated with biobanked specimens constitute a unique resource for the discovery and validation of new biomarkers and therapeutically actionable targets. Interconnecting, interoperating and sharing this data have become major issues for national health systems, raising enormous stakes as well as major societal, legal and cybersecurity challenges in terms of compliance with the protection of personal sensitive information. This book project is the second part of an initiative launched in 2012 to produce a published corpus of knowledge encompassing all aspects of human biobanking as a central practice for research and medicine. The first volume, published in 2017, is entitled: Human Biobanking: Principles and Practice. This first volume compiled a series of high level contributions overseeing the main developments that carried the progression of human biobanking as a research and biotechnological field over the past two decades. This new book project will constitute de facto Volume 2 of the same initiative, under the title: Biobanking of Human Biospecimens: lessons from 25 years of biobanking experience. Hence, the two volumes will share the same generic title (Biobanking of Human Biospecimens), with different subtitles, making clear that the two volumes are interrelated while highlighting their specificities in terms of what they actually cover. As a result, the two books are "twins" but can also be used independently of each other. The overarching aim of the two volumes of Biobanking of Human Biospecimens is to provide a published "one-stop shop" for state-of-the-art information on what constitutes the field of human biobanking, from conception of a biobank, standard operating procedures, ethical and societal aspects, governance, networking, interoperability and economic sustainability. This inclusive publication concept meets the needs of a vast readership, including scientists, doctors and technical staffs who are directly involved in biobanking operations, scientists in other disciplines that heavily rely on biobanking (such as genomics or proteomics), stakeholders and policy makers, and of course students for whom biobanking is becoming an important part of the training curriculum. So far, there has been a lack of major textbooks on biobanking. Documentation for biobanking is widely available through numerous publications, regulatory documents published by International or Governmental Agencies, and sets of recommendations essentially accessible through the Internet. However, it is difficult to access a single, top-of-the shelf reference that provides at a glance a large coverage of all aspects of human biobanking. Fulfilling this need is the main origin of the concept for this back-to-back publication project. To our knowledge, there is currently no other publication project with the same breath and scope as this one in the field of biobanking.

This book provides a comprehensive discussion on the current information and evidence on the latest developments in the field of drugs resistance. Drug resistance is the reduction in effectiveness of a medication such as an antimicrobial or an antineoplastic in treating a disease or condition. This leads to negative outcomes at great risk of public health; therefore, increasing efforts are dedicated to the development of a new generation of medications that will help deal with this phenomenon. Decades of technological innovations in drug design have demonstrated the potential of resistance. Enormous information on various aspects of antibiotics resistance is available. However, literature on drug resistance specifically related to infectious and non-infectious diseases is rarely presented, particularly those focusing on the mechanisms, biochemistry, kinetics, dynamics, and management of drug resistance. Therefore, there is an immense need for a systematic compilation on the available information about this issue. All the chapters are logically selected and arranged to provide state-of-the-art information about all aspects of drugs resistance. After an introductory chapter, four chapters are dedicated to infectious microbial diseases, whereas two other chapters are complimenting this theme and focusing on drugs resistance in ear, nose and throat, and skin diseases. The recent advances in the understanding of drugs resistance in lung, neurological, kidney, heart, and liver diseases are also covered. Biochemistry of drugs resistance in cancer, HIV, ocular, reproductive, and diabetes diseases is also discussed. Finally, a chapter dedicated to the "management of drug resistance" has been included.

Comprehensive Biomarker Discovery and Validation for Clinical Application provides the reader with an extensive introduction into all aspects of proteomics biomarker discovery, validation and development. It discusses the current status of science and technology, its limitations, bottlenecks as well as future development trends to improve the success rate of translating biomarker discovery into useful clinical tests. The most important feature of the book is to provide an overview of current technologies and the challenges encountered during biomarker discovery and validation, such as patient selection, sample handling, data processing, statistical analysis and registration and approval of validated biomarkers through European and US regulatory authorities. The authors introduce the reader to each of these topics in significant detail and provide examples or guidelines for best practice. There are prominent chapters included on biomarkers in translational and personalised medicine; an introduction to regulatory affairs and bring biomarkers to the market; biomarker discovery and the use of mass spectrometry based profiling platforms; MALDI imaging techniques in tissue-based biomarkers discovery and a clinical application study on the use of diagnostic assays for early diagnosis of heart failure using various proteomic methods. The book concludes with a final chapter on future trends in biomarker discovery and validation. The book targets a readership of industrial and academic researchers that are involved in biomarker discovery and validation or that manage biobanks, develop sample preparation methods, analytical profiling systems and bioinformatics tools. Common pitfalls and success stories in biomarker discovery are highlighted and guidelines for best practice are provided for the different parts of the procedure. The book will be an essential information resource for scientists working in the field.

These proceedings of the 2018 XIII International Symposium on Spermatology focus on comparative biology, and encourages discussion and the exchange of ideas. The aim of this Symposium was to provide a unique opportunity and bring together scientists from a wide spectrum of research fields - human, domestic animals and other mammals, vertebrates, insects, and plants. The underlying focus is on the function of the spermatozoon - a common feature for sexual reproduction, but extremely varied. By exploring the variability, a better understanding of male reproductive functions can develop. These proceedings address the mechanisms of physiology and pathophysiology, rather than diagnosis and treatment. The symposium featured keynote lectures by invited speakers, followed by presentations on specific aspects of the general topic of the session. Experimental studies are given priority over clinical studies of patient populations. The proceedings comprise both keynote speakers' texts and selected free communications. Posters were considered for publication in the proceedings, and the volume includes exhibited materials on the work of prominent spermatologists, highlighting their important past achievements in the field.

Rely on this best-selling laboratory manual to provide the hands-on practice you need to confidently perform the most common to complex respiratory procedures for class and clinical. Step-by-step instructions with detailed diagrams and illustrations seamlessly guide you through every procedure.

Completely revised and updated, the 3rd Edition reflects the latest technologies and standards of care, including the most current respiratory equipment. Plus, you'll enhance your skills with 10 new procedure videos--online at DavisPlus--demonstrating how to perform advanced respiratory procedures.

Saliva as a unique sample for health assessment is gaining attention among researchers of different fields in the last 20 years; being reflected in an impressive increase in the number of papers published studying saliva from different biological aspects in human and veterinary species. Once deemed merely a digestive juice is now considered a biological fluid capable of communicating information about physiopathological processes occurring in organisms, since saliva has been shown to contain molecular and bacterial compounds that can change in response to local and systemic pathologies. Furthermore, the interest of saliva as a diagnostic, prognostic and monitoring biofluid is forced by its non-invasive nature being of easy and inexpensive sampling, involving only minimal discomfort and allowing the collection of multiple/repeated specimens at anytime, anywhere and without need for specialized staff. In this contributed volume, the authors bring together, summarize and reflect the generated knowledge about saliva as a source of biomarkers for health and welfare evaluation in humans and animal models. This volume also highlights the importance of confounding factors, such as sampling methods, flow, total protein content, contamination, or storage. This book will serve as a manual for graduates, practitioners and researchers by providing general ideas about the possibilities and utilities of saliva in clinical practice or investigation, and indicating the main cautions each should have in mind before saliva usage.

This book provides an introduction to the principles of pharmacogenomics and precision medicine, followed by the pharmacogenomics aspects of major therapeutic areas such as cardiovascular disease, cancer, organ transplantation, psychiatry, infection, antithrombotic drugs. It also includes genotyping technology and therapeutic drug monitoring in Pharmacogenomics; ethical, Legal and Regulatory Issues; cost-effectiveness of pharmacogenetics-guided treatment; application of pharmacogenomics in drug discovery and development and clinical Implementation of Pharmacogenomics for Personalized Precision Medicine. The contributors of Pharmacogenomics in Precision Medicine come from a team of experts, including professors from academic institutions and practitioner from hospital. It will give an in-depth overview of the current state of pharmacogenomics in drug therapy for all health care professionals and graduate students in the era of precision medicine.

The textbook is based on the APPLIED use of laboratory instrumentation and apparatus in practice in the real working world with absolute minimum use of complex calculations and mathematics. Instrumental theory is kept to a minimum, with useful practical hints and unbiased instruction on lab instruments' capabilities and operations. All text is in simple to understand language of the complexities of chemical analyses.