In 2003, the word coronavirus spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors.

Comprehensive and cutting-edge, SARS- and Other Coronaviruses Laboratory Protocols serves as an ideal guide for all virologists and especially for those working with coronaviruses."

Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers.

The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.

In recent years, many animal-derived polymers have emerged as an attractive category of naturally derived polymers because of their advantageous physicochemical, chemical, and biological properties. The important biological properties of these natural polymers derived from animals are biocompatibility and biodegradation. These polymers are generally composed of repeated units of amino acids. Moreover, these polymers can be modified physically and/or chemically to improve their biomaterial properties. Natural Polymers for Pharmaceutical Applications, Volume 3: Animal-Derived Polymers looks at how these polymers can be exploited as pharmaceutical excipients in various pharmaceutical dosage forms, like microparticles, nanoparticles, ophthalmic preparations, gels, implants, etc. The commonly used animal-derived polymers used as pharmaceutical excipients are hyaluronic acid (hyaluronan), albumin, collagen, gelatin, chondroitin, etc.

Die Forschung und Anwendungsentwicklung in dem Bereich chemischer und biochemischer Sensoren ist weiterhin in einem schnellen Wachstum begriffen. Die Erfahrungen des letzten Jahrzehnts haben jedoch gezeigt, dass die erfolgreiche Entwicklung solcher Sensoren, die auch den harten Routinebedingungen in den vielf ltigen Anwendungsgebieten widerstehen, nur dann m glich ist, wenn Chemiker und Ingenieure kooperieren. Daher ist es das Ziel dieses Lehrbuches, sowohl Chemikern als auch Ingenieuren, Lebensmittel- und Biotechnologen in einer streng systematischen aber sehr praxisorientierten Darstellung die Technologie und die Anwendung chemischer Sensoren nahezubringen.

Der interdisziplin re Ansatz berbr ckt die unterschiedlichen Denkweisen in Chemie, Physik und Ingenieurwissenschaften erfolgreich.

A respected resource for decades, the Guide for the Care and Use of Laboratory Animals has been updated by a committee of experts, taking into consideration input from the scientific and laboratory animal communities and the public at large. The Guide incorporates new scientific information on common laboratory animals, including aquatic species, and includes extensive references. It is organized around major components of animal use: *Key concepts of animal care and use. The Guide sets the framework for the humane care and use of laboratory animals. *Animal care and use program. The Guide discusses the concept of a broad Program of Animal Care and Use, including roles and responsibilities of the Institutional Official, Attending Veterinarian and the Institutional Animal Care and Use Committee. *Animal environment, husbandry, and management. A chapter on this topic is now divided into sections on terrestrial and aquatic animals and provides recommendations for housing and environment, husbandry, behavioral and population management, and more. *Veterinary care. The Guide discusses veterinary care and the responsibilities of the Attending Veterinarian. It includes recommendations on animal procurement and transportation, preventive medicine (including animal biosecurity), and clinical care and management. The Guide addresses distress and pain recognition and relief, and issues surrounding euthanasia. *Physical plant. The Guide identifies design issues, providing construction guidelines for functional areas; considerations such as drainage, vibration and noise control, and environmental monitoring; and specialized facilities for animal housing and research needs. The Guide for the Care and Use of Laboratory Animals provides a framework for the judgments required in the management of animal facilities. This updated and expanded resource of proven value will be important to scientists and researchers, veterinarians, animal care personnel, facilities managers, institutional administrators, policy makers involved in research issues, and animal welfare advocates.

This volume on scientific advances in critical care testing com piles a number of clinical and laboratory studies related to criti cally ill patients that involve new technology, therapy options, application or interpretation of new tests, patient management and cost benefits. There were a total of 340 applicants for this first International Federation of Clinical Chemistry-AVL Award, and from these, National Winners were selected in 26 member countries of the IFCC. This publication presents the full papers of the ten finalists among the National Winners selected for the international final held in London in July 1996. These ten were chosen by the Inter national Awards Committee of the IFCC. In addition, the editors have decided also to include their choice of the best abstracts from the National Winners, thus giving a broad overview of cur rent research being conducted in the field of critical care medi cine among IFCC members. In keeping with the title of this volume, all major fields of in tensive care medicine are represented, including inflammation, infection, stress, hypoxia, ischaemia, cardiology, haemodynamics, blood gases, electrolytes, trace -elements, nephrology, gastroenter ology and haematology. In addition, there is also a chapter on new technology in critical care testing and on miscellaneous top ics."

The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine. Since its description in 1985, PCR has undergone tremendous improvements, and many variations on the basic PCR theme have been published. With such a large volume of PCR-related literature, a clinical scientist wishing to use the technique will have a difficult task loc- ing the relevant information to implement it effectively. There is thus clearly a need for an up-to-date volume with detailed protocols to facilitate the setting up of those techniques most relevant to clinical applications. Unlike some other books on this topic, Clinical Applications of PCR includes only methods that are of direct relevance in clinical settings. The book is organized in three parts: an introductory section, a section on general methodology, and a final section with specific clinical applications. The first section covers the basic principles of PCR and is most useful to those new to molecular diagnosis. The next chapter includes useful tips for setting up a PCR laboratory. Section 2 then outlines some of the most commonly used PCR-based techniques in molecular diagnosis. Section 3 includes carefully chosen examples that represent typical applications of PCR in diverse clinical fields, encompassing hematology, oncology, genetics, and microbiology.

In Vitro Toxicology Systems brings together important issues and considerations needed in order to develop a workable, reliable, integrated testing strategy for the replacement of animals in toxicity testing regimes. This thorough volume includes sections on in vitro models for systemic organ toxicity, neurotoxicity, sensory organs, immunotoxicity and reproductive toxicity and addresses how stem cells may be used going forward. The book also tackles difficult areas of toxicology such as carcinogenicity and nanotoxicology, with additional chapters dedicated to kinetics, metabolism, and in vitro in vivo extrapolation. The book also addresses biological processes such as stress response pathways and mechanistic biomarkers and how these can be uncovered and measured using high content approaches. Reliable and authoritative, In Vitro Toxicology Systems will be of benefit not only to students, scientists and regulators working in the field of chemical safety assessment but also to a wider scientific audience.

This new edition focuses on a variety of techniques available for the analysis of drugs in biological fluids. Over 150 figures and tables help to describe the latest advances and give examples of their applications. Current chiral analysis methods as well as discussions on the impact of chirality are described. Practical aspects of bioanalytical work, including many examples of laboratory problems not often reported in the scientific literature, are examined in depth.

Recent years have seen a greater industrial emphasis in undergraduate and postgraduate courses in the pharmaceutical and chemical sciences. However, textbooks have been slow to adapt, leaving the field without a text/reference that is both instructional and practical in the industrial setting - until now. A Handbook of Bioanalysis and Drug Metabolismis a stimulating new text that examines the techniques, methodology, and theory of bioanalysis, pharmacokinetics, and metabolism from the perspective of scientists with extensive professional experience in drug discovery and development. These three areas of research help drug developers to optimize the active component within potential drugs thereby increasing their effectiveness, and to provide safety and efficacy information required by regulators when granting a drug license. Professionals with extensive experience in drug discovery and development as well as specialized knowledge of the individual topics contributed to each chapter to create a current and well-credentialed text. It covers topics such as high performance liquid chromatography, protein binding, pharmacokinetics and drug-drug interactions. The unique industrial perspective helps to reinforce theory and develop valuable analytical and interpreting skills. This text is an invaluable guide to students in courses such as pharmaceutical science, pharmacology, chemistry, physiology and toxicology, as well as professionals in the biotechnology industry.

Contains a DVD that includes more than 50 videos of surgical and experimental techniques and a color histologic atlas of normal tissues Provides normal data for the most common breeds of minipigs and domestic swine Adds new chapters on cancer models, transgenics, necropsy, and toxicology Includes technical discussions on anesthesia, analgesia, and perioperative care that represent the best practices for using swine in experimental surgery Presents extended reference lists at the end of each chapter to point toward further study

Die grundsatzlichen Moglichkeiten und Grenzen der Bewertung des Nachweisvermogens von Analysenverfahren und -ergebnissen werden dargestellt. Diesbezugliche Leistungskriterien haben Bedeutung fur die Auswahl und den Vergleich von Verfahren fur die Spurenanalyse inbezug auf praktische Aufgabenstellungen, die Interpretation von Analysenergebnissen sowie die Entwicklung, Optimierung und Validierung von Analysenverfahren. Die gegenwartige Vielzahl von teilweise widerspruchlichen Bezeichnungsarten und Berechnungsweisen der eingefuhrten charakteristischen Kenngrossen fuhrt zu Verwirrungen und Fehldeutungen und somit zu Unsicherheiten sowohl im Verstandnis als auch in praktischen Anwendungen."

The seventh edition of this concise, highly practical guide to the interpretation of normal and abnormal laboratory results is fully revised and expanded, with updates on established and familiar tests, as well as interpretations on recent developments. With increasing responsibility being placed on primary care, the book includes guidelines on specific clinical conditions such as heart failure, management of female infertility, specific lipid monitoring in diabetes and guidance for monitoring renal failure. It also includes suggestions for appropriate laboratory tests in certain clinical situations, for example: dementia screen, screening tests when a patient presents with a neuropathy, and appropriate tests for patients presenting with hypertension, chronic fatigue syndrome, erectile dysfunction and gynaecomastia. Completely up-to-date, A Guide to Laboratory Investigations, Seventh Edition remains an essential handbook for all primary care professionals professionals and a valuable reference for medical students and hospital physicians in training and in practice.

Clinical Chemistry: Principles, Techniques, and Correlations, Ninth Edition is the most student-friendly clinical chemistry text available today.

The Ninth Edition keeps students at the forefront of what continues to be one of the most rapidly advancing areas of laboratory medicine with clear explanations that balance analytic principles, techniques, and correlation of results with coverage of disease states.

The book not only demonstrates the how of clinical testing, but also the what, why, and when of testing correlations to help students develop the knowledge and interpretive and analytic skills they'll need in their future careers.

Jede medizinische Fachrichtung benoetigt labormedizinische Diagnostik. Neben bildgebenden Verfahren ist sie die wichtigste Hilfsdisziplin im medizinischen Alltag. Die Anzahl der Daten und Befunde steigt rapide an. Ursachen hierfur sind die Automatisierung und die Einfuhrung neuer Methoden. Anhand von Fallbeispielen werden Laborbefunde interpretiert und die Aussagekraft einzelner Werte erlautert. Raschen UEberblick ermoeglicht die Gliederung der klinischen Fragestellungen je nach Fachrichtung. Eine praktische Einfuhrung fur Studenten und AErzte in Ausbildung sowie fur Personen, die im medizinisch-diagnostischen Bereich tatig sind.

This book presents the latest data from basic research and clinical trials supporting the effectiveness of ketamine as a treatment for depression, bipolar disorder, and suicidal behavior, setting these positive findings within the context of the serious problem of ketamine abuse. The first part of the book focuses on the evidence regarding ketamine abuse, with specific reference to Asian countries, and discusses countermeasures and complication management. It then addresses the mechanisms underlying the antidepressant and side effects of ketamine, which have remained elusive, describing and discussing important new research findings. Further, it explains insights gained from whole brain imaging in rodents and from behavioral pharmacology, and presents evidence regarding the role of gut microbiota, the NMDA receptor GluN2D subunit, and the lateral habenula in the actions of ketamine. These advances form the basis for the safer use of ketamine in patients with treatment-resistant depression and are expected to lead to the development of new antidepressants.

The perfect balance of theory and practice! Here's the practical introduction you need to understand the essential theoretical principles of clinical immunology and the serological and molecular techniques commonly used in the laboratory. You'll begin with an introduction to the immune system; then explore basic immunologic procedures; examine immune disorders; and study the serological and molecular diagnosis of infectious disease. An easy-to-read, student-friendly approach emphasizes the direct application of theory to clinical laboratory practice. Each chapter is a complete learning module with learning outcomes, chapter outlines, theoretical principles, illustrations, and definitions of relevant terminology. Review questions and case studies help you assess your mastery of the material. A glossary at the end of the book puts must-know information at your fingertips. An access code inside new printed texts unlocks Lab Exercises and Branching Case Studies online at FADavis.com that offer more opportunities to apply theory to clinical laboratory practice.

An essential companion for both the aspiring and practising electrophysiologist, The EHRA Book of Pacemaker, ICD and CRT Troubleshooting assists device specialists in tackling both common and unusual situations that that they may encounter during daily practice. Taking a case-based approach, it examines pacemakers, implantable cardioverter defibrillators and cardiac resynchronisation therapy. Much more than just a technical manual of device algorithms, the cases help readers to consolidate their technical knowledge, and improve their reasoning and observation skills so they are able to tackle device troubleshooting with confidence. The 70 cases are arranged in three sections by increasing levels of difficulty to walk readers through all the skills and knowledge they need in an easy to use and structured format. Each case contains a short clinical description and a device tracing followed by a multiple choice question. Answers are supplied with detailed annotations of the tracing and an in-depth discussion of the case, highlighting practical hints and tips as well as providing an overview of the technical function of devices. A useful summary of principal device features and functions is also included. The EHRA Book of Pacemaker, ICD and CRT Troubleshooting is the perfect companion for electrophysiologists, cardiology trainees and technical consultants working with device patients as well as for those studying for the EHRA accreditation exam in cardiac pacing. This print edition of The EHRA Book of Pacemaker, ICD, and CRT Troubleshooting comes with a year's access to the online version on Oxford Medicine Online. By activating your unique access code, you can read and annotate the full text online, follow links from the references to primary research materials, and view, enlarge and download all the figures and tables. Oxford Medicine Online is mobile optimized for access when and where you need it.

The present work is an attempt to provide a systematic treatment of genetic linkage in diploid heredity. Part A presents a general account of statistical methods which can be brought to bear on the problem. The primary emphasis is on the practical aspects of estimation. A large proportion, if not the majority, of mutant genes fail to match up to 'textbook' genes-with faultless segregation ratios and expression-yet, these are the materials with which the practical researcher has to cope. For this reason, it is important to know how to deal with the assortment of genes which may display significant deviations from expectation. Part B examines the accumulated data on linkage for most of the laboratory mammals and provides a comprehensive and up-to-date survey. The need for a critical review has often been expressed and it is hoped that the present analysis will fill the gap. The volume of material is probably the most important in the animal kingdom other than that for Drosophila species.

The discovery of dopamine in 1957-1958 was one of the seminal events in the development of modern neuroscience, and has been extremely important for the development of modern therapies of neurological and psychiatric disorders. Dopamine has a fundamental role in almost all aspects of behavior: from motor control to mood regulation, cognition and addiction and reward, and dopamine research has been unique within the neurosciences in the way it has bridged basic science and clinical practice. Over the decades research into the role of dopamine in health and disease has been in the forefront of modern neuroscience. The Dopamine Handbook is the first single-volume publication to capture current progress and excitement in this dynamic research field.

Specifically intended for lab-based biomedical researchers, this practical guide shows how to design experiments that are reproducible, with low bias, high precision, and widely applicable results. With specific examples from research using both cell cultures and model organisms, it explores key ideas in experimental design, assesses common designs, and shows how to plan a successful experiment. It demonstrates how to control biological and technical factors that can introduce bias or add noise, and covers rarely discussed topics such as graphical data exploration, choosing outcome variables, data quality control checks, and data pre-processing. It also shows how to use R for analysis, and is designed for those with no prior experience. An accompanying website (https://stanlazic.github.io/EDLB.html) includes all R code, data sets, and the labstats R package. This is an ideal guide for anyone conducting lab-based biological research, from students to principle investigators working in either academia or industry.

Initially genetic disorders were all considered as rare diseases. At present, in the mid of 2009, the OMIM catalogue contains information on more than 12 000 entries of which about 2500 are available for clinical testing based on the identification of the responsible gene defect. However, altogether it has been estimated that about 8 percent of a population in the economically developed countries will during their lifetime suffer from a disease mainly as the result of their genetic constitution. Adding to that, it is estimated that all diseases have a genetic component, which will determine who will be at a higher than average risk for a certain disorder. Further it is postulated that in the near future, this genetic profiling could become useful in selecting an appropriate therapy adapted to the genetic constitution of the person. Thus, genetic disorders are not rare.

Measuring quality of health care related processes became an issue in the 1990s, mainly in laboratory medicine, but also for hospitals and other health care systems. In many countries national authorities started to implement recommendations, guidelines or legal procedures regulating quality of health care delivery. In laboratory medicine, in parallel, the use of accreditation as a method assuring high quality standards in testing came in use. With the increasing possibilities of performing molecular genetic testing, genetic laboratories needed to become involved in this process.

As many genetic disorders are rare, most laboratories worldwide offered analysis for a specific set of disorders, and, therefore, very early on a transborder flow of samples occurred. While international quality criteria (ISO) have been in existence for a number of years, the regulation of quality issues still may differ between countries.

Based on their personal experience in the varying fields of quality research and clinical implementation of quality criteria in genetic services the authors of this book share their experience and give examples of the implementation of quality issues in national quality systems worldwide. This book, which is the result of the effort of many persons, is destined to aid laboratory managers and counsellors, health care managers and other stakeholders in national or international health care service to improve the services to the benefit of patients with suspected genetic disorders.

Use THE definitive reference for laboratory medicine and clinical pathology! Tietz Textbook of Laboratory Medicine, 7th Edition provides the guidance necessary to select, perform, and evaluate the results of new and established laboratory tests. Comprehensive coverage includes the latest advances in topics such as clinical chemistry, genetic metabolic disorders, molecular diagnostics, hematology and coagulation, clinical microbiology, transfusion medicine, and clinical immunology. From a team of expert contributors led by Nader Rifai, this reference includes access to wide-ranging online resources on Expert Consult - featuring the comprehensive product with fully searchable text, regular content updates, animations, podcasts, over 1300 clinical case studies, lecture series, and more. Authoritative, current content helps you perform tests in a cost-effective, timely, and efficient manner; provides expertise in managing clinical laboratory needs; and shows how to be responsive to an ever-changing environment. Current guidelines help you select, perform, and evaluate the results of new and established laboratory tests. Expert, internationally recognized chapter authors present guidelines representing different practices and points of view. Analytical criteria focus on the medical usefulness of laboratory procedures. Use of standard and international units of measure makes this text appropriate for any user, anywhere in the world. Expert Consult provides the entire text as a fully searchable eBook, and includes regular content updates, animations, podcasts, more than 1300 clinical case studies, over 2500 multiple-choice questions, a lecture series, and more. NEW! 19 additional chapters highlight various specialties throughout laboratory medicine. NEW! Updated, peer-reviewed content provides the most current information possible. NEW! The largest-ever compilation of clinical cases in laboratory medicine is included on Expert Consult. NEW! Over 100 adaptive learning courses on Expert Consult offer the opportunity for personalized education.