Principles of Animal Research is the first publication to offer a broad look at animal research science for a student, early researcher, or technician. Offering guidance for all aspects of the research experience, including the research and development of a thesis, model selection, experimental design, IACUC protocol preparation, and animal husbandry and technical procedural needs, the book is a necessary addition to every student, technician, and researcher's education.

Advances in Clinical Chemistry, Volume 77, the latest installment in this internationally acclaimed series, contains chapters authored by world-renowned clinical laboratory scientists, physicians, and research scientists. The serial discusses the latest and most up-to-date technologies related to the field of clinical chemistry and is the benchmark for novel analytical approaches in the clinical laboratory.

Atlas of Histology of the Juvenile Rat should be of interest to toxicologic pathologists, toxicologists, and other biological scientists who are interested in the histomorphology of juvenile rats. For several decades the laboratory rat has been used extensively in nonclinical toxicology studies designed to detect potential human toxicity of drugs, agrochemicals, industrial chemicals, and environmental hazards. These studies traditionally have involved young adult rats that are 8-10 weeks of age as studies are started. It is becoming increasingly apparent that children and young animals may have different responses to drug/chemical exposures, therefore, regulatory agencies are emphasizing toxicology studies in juvenile animals. While the histologic features of organs from young adult and aged laboratory rats are well known, less is known about the histologic features of organs from juvenile rats. Final histologic maturity of many organs is achieved postnatally, thus immature histologic features must be distinguished from chemical- or drug-related effects. While this postnatal organ development is known to exist as a general concept, detailed information regarding postnatal histologic development is not readily available. The Atlas includes organs that are typically sampled in nonclinical toxicology studies and presents the histologic features at weekly intervals, starting at birth and extending through postnatal day 42.

This book is part of a series of titles that are a spin-off of the Shingo Prize-winning book Leveraging Lean in Healthcare: Transforming Your Enterprise into a High Quality Patient Care Delivery System. Each book in the series focuses on a specific aspect of healthcare that has demonstrated significant process and quality improvements after a Lean implementation. Lean principles can help medical laboratories drive up efficiencies and quality without increasing costs or compromising quality. Leveraging Lean in Medical Laboratories: Creating a Cost Effective, Standardized, High Quality, Patient-Focused Operation provides a functional understanding of Lean laboratory processes and quality improvement techniques. This book is an ideal guide for healthcare executives, leaders, process improvement team members, and inquisitive frontline workers who want to implement and leverage Lean in medical laboratories. Supplying detailed descriptions of Lean tools and methodologies, it identifies powerful Lean solutions specific to the needs of the medical laboratory. The first section provides an overview of Lean concepts, tools, methodologies, and applications. The second section focuses on the application of Lean in the laboratory environment. Presenting numerous examples, stories, case studies, and lessons learned, it examines the normal operation of each area in the lab environment and highlights the areas where typical problems occur. Next, it walks readers through various Lean initiatives and demonstrates how Lean tools and concepts have been used to achieve lasting improvements to processes and quality of care. It also supplies actionable blueprints that readers can duplicate or modify for use in their own institutions. Illustrating leadership's role in achieving departmental goals, this book will provide you with a well-rounded understanding of how Lean can be applied to achieve significant improvements throughout the entire continuum of care.

The rapid acceptance of immunohistochemistry as an important and even indispensable adjunct to morphological examination and diagnosis requires the modern anatomical pathology laboratory to be conversant with, and proficient in, immunostaining procedures, as well as methods of tissue processing and antigen retrieval and the underlying characteristics of the increasing number of antibodies and antisera available. This fully revised, expanded and updated edition provides a comprehensive list of antisera and monoclonal antibodies that have useful diagnostic applications in tissue sections and cell preparations. Various clones, which are commercially available to detect the same antigen, are listed and the sensitivities and specificities of the antibodies are discussed. Importantly, the authors, all pathologists with a wealth of experience in immunostaining procedures, provide fully referenced details and expert advice on each reagent, which the reader will find invaluable. Finally, the appendices provide easily accessible and clear summaries of selected antibody panels for specific diagnostic situations, details of heat-induced antigen/epitope retrieval (including the use of microwaves) and a useful reference to the websites of the main antibody suppliers.

Research institutions have or are planning to build, expand and renovate animal research facilities to keep up with demands of biomedical research caused in part by growth in the use of genetically altered rodents and the upsurge of research in infectious diseases.
Properly designed facilities greatly facilitate effective management and high quality day-to-day animal care that is required to optimally support animal research and testing. There are multiple solutions to address the myriad of factors that influence the design and construction of research animal facilities. There is no "best" design applicable for all facilities and arguably not even a single "best" design for a given facility. For this reason, this is not intended to be a "how to" book. The goal is to cover the basic programmatic requirements of animal research facilities, provide ideas for meeting those requirements while, hopefully, stimulating the creative process in which designers in consultation with those who work in research animal facilities generate even better ideas. That is how progress has been made and will continue to be made.
* Focuses primarily on biomedical research and development facilities
* Facilitates communication between the various disciplines involved in planning and designing animal facilities by providing contemporary information, and stimulating creativity that will help lead to wise decisions and advance the knowledge base for planning, design and constructing research animal facilities

Changes in the organization of health services in developing countries have led to the district level assuming more responsibility for the planning, delivery and quality of community health care. This fully up-dated new edition has been produced to help those working in the district laboratory, and those responsible for the organization and management of community laboratory services and the training of district laboratory personnel. Replacing the previous publication Medical Laboratory Manual for Tropical Countries, this book provides an up-to-date practical bench manual, taking a modern approach to the provision of a quality medical laboratory service. It includes practical accounts of: organization and staffing of district laboratory services; total quality management; health and safety; equipping district laboratories; parasitological tests, illustrated in colour; clinical chemistry tests; how to plan a training curriculum for district laboratory personnel. Volume 2, published in late 1999, covers microbiological tests, haematological tests and blood transfusion tests.

This is the first book in a series that serves the dual purpose of preparing
students of CLS programs for certification exams and giving cross training
skills to established clinical lab scientists who now find themselves called
on to work in other areas of the lab. This book uses a building block
approach to convey the material in a logical fashion. Starting with an
overview of medicine and the medical laboratorys relationship, the text
proceeds to the diagnostic value of those analytes normally associated with
clinical chemistry, on to technologies of analysis, quality assurance, and
statistical analysis of laboratory data. A&L Outline Review of Clinical Chemistry
makes it easy to optimize your study sessions and
use your time efficiently, and helps you quickly locate key concepts. This
PREP guide provides pretests to help you identify areas for extra attention,
and gives you what you need to know in an easy-to-remember format, packed
with study-enhancing devices such as illustrations and learning objectives.

DNA fingerprinting is a revolutionary technique that enables scientists to match minute tissue samples and facilitates scientific studies on the composition, reproduction, and evolution of animal and plant populations. As a tool for positive identification of criminals, it plays a particularly important role in forensic science. The first book to be published in the field, , DNA Fingerprinting is a practical guide to basic principles and laboratory methods as applied to a variety of fields including forensic analysis, paternity testing, medical diagnostics, animal and plant sciences, and wildlife poaching.

A growing body of medical research suggests that many medical tests used for diagnosis, prognosis, and monitoring response to treatment are unnecessary or inappropriate, yield inaccurate or misleading results, and add to the incidence of iatrogenic disease. Many such tests go into common clinical use without convincing documentation of their value because current methods for evaluating medical tests produce inconsistent results. Evaluating Medical Tests presents a systematic, objective methodology by which to determine the effectiveness of medical tests. Kraemer clearly and concisely shows how to define statistical terms and approaches consistently from study to study, how to stipulate statistical assumptions underlying various approaches, how to check for empirical validity, and how to judge the robustness of statistical outcomes, resulting in models that integrate many different approaches and extend the strengths of each. Statisticians, evaluators, researchers, practitioners, policymakers, and advanced students concerned with the evaluation of medical tests used to make clinical decisions for patient care and for research will find this essential, thought-provoking reading. "This volume is a pleasure to read. It succinctly combines carefully reasoned conceptualization with the mathematical methods for addressing test evaluation problems. With clinically relevant, illustrative examples, it draws on the author's extensive experience and research in biomedical statistics. It should be required reading for medical students and practitioners. It is invaluable to researchers and statisticians, and it provides a logical, scientific basis for efforts at medical cost containment. It is a classic! --from the Foreword by A. John Rush, M.D. "This fascinating book integrates a wide range of statistical and methodological concepts, reflects a thorough introduction to the issues confronted by evaluators of [medical] tests, and challenges motivated readers to adopt these procedures in their own research. This is an exciting book, with obvious strong policy implications concerning the way that [medical] tests are evaluated and the need for standards in this regard. . . . Evaluating Medical Tests should be of interest to all professionals who depend on tests as an adjunct in the decision-making process." --Paul R. Yarnold Northwestern University Medical School "Dr. Kraemer has undertaken a most massive and laudable task in writing Evaluating Medical Tests. . . . Dr. Kraemer's book stands as a flagship for those concerned to understand the reasons behind our escalating health care costs and the implications this holds for every American. . . . This leads us to one of the innovative features of Dr. Kraemer's book: 'This is not merely a statistical issue, or merely a medical issue, it is a national policy issue of the greatest importance.'. . . She does not assume that her readers are either statisticians or physicians. As such, she skillfully guides one through the terrain of medical and statistical jargon and points our where the assumptions of the statistics are either not stipulated or incongruent with the medical test and data being evaluated, thus rendering results and interpretations suspect. Another plus of Dr. Kraemer's writing is her willingness to use '. . . the first person singular to differentiate . . . comments from presentations of what I would consider provable or documentable facts. . . ' In so doing, she raised issues and calls medical professionals, policymakers, and patients into dialogue to consider the impact of the use of these various techniques in diagnosis. . . . Evaluating Medical Tests is of specific relevance to rehabilitation counselors who proceed with their clients through the maze of diagnostic and prognostic tests. . . . It is especially important for rehabilitation counselors who act as expert witnesses to have a working knowledge of this material to enhance their discussions with health care personnel." --Journal of Applied Rehabilitation Counseling

This volume collects essays by the late bioethicist John D. Arras, best known for his many contributions to the methodology of bioethics. Always open-minded, Arras did not favor a single theory or view of method in bioethics, eschewing labels such as "casuist" or "pragmatist." He was conversant with the main philosophical methods that have dominated bioethics since the field's origin, including principlism, Gert's common morality, the "new casuistry", pragmatism, and others. Rather than defending any particular theory or method, though, Arras rigorously investigated those methods - and how they both expand and limit our field of vision. He sought, in the tradition of Kierkegaard, to make life "harder" for bioethics, by uncovering challenges to the field's analytical methods. His favorite mode of exploration and expression was the thoughtful essay. The essays collected here reveal him thinking through new problems and new possibilities, and they invariably yield fresh and valuable insights.

The field of Chemical Engineering and its link to computer science is in constant evolution and new engineers have a variety of tools at their disposal to tackle their everyday problems. Introduction to Software for Chemical Engineers, Second Edition provides a quick guide to the use of various computer packages for chemical engineering applications. It covers a range of software applications from Excel and general mathematical packages such as MATLAB and MathCAD to process simulators, CHEMCAD and ASPEN, equation-based modeling languages, gProms, optimization software such as GAMS and AIMS, and specialized software like CFD or DEM codes. The different packages are introduced and applied to solve typical problems in fluid mechanics, heat and mass transfer, mass and energy balances, unit operations, reactor engineering, process and equipment design and control. This new edition offers a wider view of packages including open source software such as R, Python and Julia. It also includes complete examples in ASPEN Plus, adds ANSYS Fluent to CFD codes, Lingo to the optimization packages, and discusses Engineering Equation Solver. It offers a global idea of the capabilities of the software used in the chemical engineering field and provides examples for solving real-world problems. Written by leading experts, this book is a must-have reference for chemical engineers looking to grow in their careers through the use of new and improving computer software. Its user-friendly approach to simulation and optimization as well as its example-based presentation of the software, makes it a perfect teaching tool for both undergraduate and master levels.

• Minimal Text. Specific details are available in the textbook. We will only provide text to set up and support the activities in the lab.
• Variety of Learning Activities. Within each lab exercise will be a variety of activities, including coloring and labeling anatomical figures, critical thinking and clinically-orientated questions that promote problem-solving.
• Effective Use of Illustrations. Simple line drawings, that allow important features to stand out, not just duplication of figures from the text book, will support the lab activities. Histology specific to each organ system is found in that system chapter, not all lumped together in a separate section.
• Active Diagrams. Many diagrams will be provided without labels, or in a coloring-book format to promote active learning of the material.

Dieses Lehrbuch bietet Antworten auf Fragen wie "Wie funktioniert diese mikrobiologische Methode ganz praktisch? Wie kann ich meine Forschung um eine sinnvolle Methode erweitern?" Vergleichbar einem Kochbuch sind Zutaten und einzelne Handgriffe ubersichtlich aufgelistet und mit praktischen Tipps und Gefahrenhinweisen versehen. Ob zu Fluoreszenzmikroskopie, Genomik oder Isolierung und Kultivierung von Bakterien, Archaeen und Pilzen - erfahrene MikrobiologInnen finden hier ebenso schnelle Hinweise wie Quereinsteiger, die ihr Arbeitsgebiet mikrobiologisch erweitern wollen. Die fachkundigen PraktikerInnen Astrid Brandis-Heep, Erika Kothe und Timo Zimmermann haben ubersichtlich praktische Methoden und Ratschlage fur den Laboralltag zusammengestellt, die - mikrobiologisches Grundwissen vorausgesetzt - Masterstudierenden, Doktoranden, Postdocs und fortgeschrittenen Arbeitsgruppen sowohl im Alltag als auch fur eine Neuausrichtung Ihrer Forschung entscheidende Hinweise geben.

Desde su primera edicion, hace ya casi 40 anos, Koneman. Diagnostico microbiologico. Texto y atlas se ha convertido en la obra de referencia indiscutible para el diagnostico etiologico de las enfermedades infecciosas. Su contenido hace un abordaje completo de los fundamentos y de los procedimientos de laboratorio para la identificacion microbiologica aplicada a la bacteriologia, micologia y parasitologia, incluyendo los metodos tradicionales de aislamiento y los mas novedosos basados en reacciones inmunologicas y moleculares. Estudiantes, catedraticos investigadores y profesionales del diagnostico microbiologico y de las ciencias de la salud interesados en la identificacion y el estudio de las enfermedades infecciosas se beneficiaran con el contenido completo y de facil comprension de esta obra, asi como por la gran cantidad de ilustraciones. Esta 7.a edicion ha sido totalmente actualizada y mejorada con nuevos recursos pedagogicos, escenarios clinicos, fotografias, ilustraciones y recursos adicionales para estudiantes y docentes. Caracteristicas destacadas: Contenido revisado y actualizado Incluye un recuento completo de las tecnicas mas recientes de identificacion microbiologica basadas en tecnicas moleculares e inmunologicas Aplicaciones de microbiologia clinica y diagnostica en un solo volumen Principios de pruebas bioquimicas a detalle Inserto a color al final del libro con laminas que muestran distintos tipos de microorganismos Contenido exclusivo disponible en linea en thePoint : Para estudiantes : protocolos Para profesores : banco de imagenes, presentaciones en PowerPoint (R), generador de examenes y contenido disponible para sistemas de gestion de aprendizaje (LMS)

This book provides a comprehensive, state-of-the-art account of the role of immunohistochemistry in the diagnosis of skin tumors, which is crucial given that overlapping histologic features and unusual morphologic changes can lead to considerable diagnostic uncertainty. The book reviews in detail the sensitivity and specificity of commonly available antibodies and their pattern of immunostaining. readers will learn when to order antibodies and how to interpret findings. In addition, prognostic markers are evaluated and emphasis placed on the pitfalls commonly encountered when evaluating these neoplasms. The text is complemented by a wealth of superb images. Helpful histograms and algorithms are included, and clear guidance is provided on the application and interpretation of less commonly used antibodies and immunostains. Applied Immunohistochemistry in the Evaluation of Skin Neoplasms will serve as an extremely valuable resource for practicing dermatopathologists and pathologists.

In recent decades, dendrimers - free-shaped synthetic macromolecules - have garnered a great deal of scientific interest because of their unique molecular nanostructure. Used in a variety of scientific applications, dendrimers are now widely regarded as a safer, more precise and more effective way to practice medicine. This book compiles and details cutting-edge research in science and medicine from the interdisciplinary team of the Michigan Nanotechnology Institute for Medicine and Biological Sciences, which is currently revolutionizing drug delivery techniques through the development of engineered nanodevices. Edited by Istvan J. Majoros and James R. Baker Jr, two prominent nanotechnology researchers, this book will appeal to anyone involved in nanotechnology, macromolecular science, cancer therapy or drug delivery research.

Now in its third edition, Introduction to Immunocytochemistry is an essential text for newcomers to the field, and will be a valuable reference for more experienced workers. Extensively expanded and updated, this volume covers both background theory and practical applications, including immunostaining methods using fluorescent, enzyme and gold labels.

Ce jeu de douze planches plastifiees au format A4 est produit pour aider au diagnostic du paludisme humain par l'examen au microscope. Les planches sont concues dans un double but: apporter une aide pratique aux agents de sante dans leurs activites quotidiennes de diagnostic des infections paludiques et une aide pedagogique aux etudiants et stagiaires. Ce nouveau jeu a ete produit en tenant compte des observations et commentaires faits par un grand nombre de professionnels et d'experts qui ont utilise la deuxieme edition des Planches pour le diagnostic du paludisme (OMS, 2000).On y trouve des photographies au microscope qui montrent les differentes especes et morphologies du paludisme dans les frottis minces et epais. Elles fournissent des descriptions de P. falciparum, P. vivax, P. ovale et P. malariae, ainsi que des instructions detaillees sur la preparation et l'utilisation des solutions tampons et des solutions de coloration. Les photographies au microscope, toutes realisees au grossissement x 1000 et avec des colorants de Romanowsky, montrent nombre des variations possibles des parasites du paludisme. On y trouvera egalement des illustrations et des explications des erreurs courantes dans la preparation des frottis sanguins, de meme que des conseils pour la manipulation sans danger des echantillons de sang, afin d'eviter le plus possible toute exposition aux virus de l'hepatite et au VIH/sida. De nouvelles sections ont ete ajoutees sur les anticoagulants et le diagnostic des infections mixtes, ainsi que sur la reconnaissance d'autres parasites courants dans le sang peripherique. Les planches sont produites sous une forme plastifiee et etanche qui les rend solides et faciles a utiliser sur le terrain, comme sur la paillasse du laboratoire."

Lieber EXPERIMENTATOR, dieses Buch vermittelt Ihnen das nAtige Grundlagenwissen und ist eine Orientierungshilfe im verwirrenden Dschungel von Zellen, Medien, Seren, GerAten und Vorschriften. Anschauliche Vergleiche und Beispiele sowie der lockere Schreibstil machen es zu einer informativen und spannenden LektA1/4re. HAufig auftretende Probleme (z.B. Kontaminationen in der Zellkultur) werden eingehend behandelt und LAsungsvorschlAge angeboten. Die 2. Auflage wurde um das Thema Kreuzkontaminationen und falsch identifizierte Zelllinien erweitert. Schwerpunkte wie Steriltechnik, Subkultur, AdhAsion und Detachment, Kryokonservierung und Langzeitlagerung von Zellen sowie zellbiologische Methoden werden verstAndlich dargestellt. Der Einsatz neuer Techniken wie siRNA wird an einem aktualisierten Praxisbeispiel erklArt und um einen Beitrag zur in vitro-Differenzierung mesenchymaler Stammzellen ergAnzt.

DarA1/4ber hinaus enthAlt das Buch eine A1/4berarbeitete Sammlung von Ressourcen fA1/4r die Beschaffung von Zelllinien, Kontaktadressen fA1/4r Fortbildungskurse und Dienstleistungsangebote rund um die Zellkultur sowie Listen informativer Datenbanken und nA1/4tzlicher Adressen. Solch ein umfangreicher Fundus lAdt Sie zum StAbern und SchmAkern ein und bietet auch dem erfahrenen Zellkultur-Profi neue Informationen. Diese Auflage bietet einen Aoeberblick A1/4ber den neuesten Stand von Verordnungen und Regelwerken.

Das Buch richtet sich an Studierende, Praktikanten, technische Assistent(inn)en, Diplomanden, Doktoranden und an alle, die sich neben einem "Kochbuch" zur Zellkultur einen Begleiter im Laboralltag wA1/4nschen, der Hilfestellung bei Problemen anbietet, erst recht wenn Murphys Gesetz mal wieder zugeschlagen hat.

The book brings together experts working in public health and multi-disciplinary areas to present recent issues in statistical methodological development and their applications. This timely book will impact model development and data analyses of public health research across a wide spectrum of analysis. Data and software used in the studies are available for the reader to replicate the models and outcomes. The fifteen chapters range in focus from techniques for dealing with missing data with Bayesian estimation, health surveillance and population definition and implications in applied latent class analysis, to multiple comparison and meta-analysis in public health data. Researchers in biomedical and public health research will find this book to be a useful reference and it can be used in graduate level classes.

"A Practical Guide to Frozen Section Technique" offers an easy to learn approach to frozen section technique in the form of a highly illustrated handbook intended for onsite use in the laboratory. The book begins with a novel, clearly delineated, step by step approach to learning continuous motion brush technique. Emphasis is placed on recognizing and correcting artifacts during the preparation process. The book addresses all of the steps in the preparation of slides from cutting through cover-slipping. The author's unique, original techniques for tissue embedding including face down embedding in steel well bars, frozen block cryoembedding and paper cryoembedding are detailed.Variables key to the quality of the preparation including block temperature, tissue properties and section thickness are detailed. The book also covers understanding the cryostat and basic maintenance and care. Sections covering techniques used in Mohs dermatologic surgery, and techniques used in basic animal and human research are discussed by noted experts in their field. "A Practical Guide to Frozen Section Technique" will be of great value to pathologists, pathology residents in training and also experimental pathology researchers that rely upon this methodology to perform tissue analysis in research.

Depuis sa premiere edition en 1983, il y a plus de vingt ans, le Manuel de securite biologique en laboratoire a donne des conseils pratiques sur les techniques de securite a appliquer dans les laboratoires a tous les niveaux. La bonne application des techniques microbiologiques et l'utilisation de l'equipement de securite biologique par du personnel bien entraine restent les piliers de la securite en laboratoire. Toutefois, la mondialisation, les progres technologiques, l'apparition de nouvelles maladies et les graves menaces liees a une utilisation ou a une mise en circulation deliberees de micro-organismes ou de toxines ont impose de revoir les procedures. Pour cette nouvelle edition, le manuel a donc ete considerablement revu et developpe.Le manuel couvre desormais aussi l'evaluation du risque et l'utilisation sans risque des techniques faisant appel a l'ADN recombinant. Il donne par ailleurs des lignes directrices pour la mise en service et la certification des laboratoires. Il presente les concepts de la securite biologique et les reglementations internationales les plus recentes sur le transport des matieres infectieuses. On y a egalement integre les informations sur la securite dans les laboratoires d'analyses medicales, publiees auparavant dans d'autres documents de l'OMS. Nous esperons que le manuel continuera d'inciter les pays a instituer des programmes de securite biologique et des codes nationaux de bonnes pratiques pour manipuler sans danger les matieres potentiellement infectieuses."

"Lab Literacy for Canadian Doctors" helps you to make better decisions about the right tests for typical clinical situations, quickly and efficiently, to improve patient care. Refer to this essential guide for: Advice and information on lab errors, false positives and negatives, and blood and tissue collection. A quick index summarizing the clinical utility of common tests. Sections on dermatology, ENT and respiratory system, endocrine system, fatigue, GI and hepatic system, gynecology and pregnancy, routine screening, and more.