The rapid acceptance of immunohistochemistry as an important and even indispensable adjunct to morphological examination and diagnosis requires the modern anatomical pathology laboratory to be conversant with, and proficient in, immunostaining procedures, as well as methods of tissue processing and antigen retrieval and the underlying characteristics of the increasing number of antibodies and antisera available. This fully revised, expanded and updated edition provides a comprehensive list of antisera and monoclonal antibodies that have useful diagnostic applications in tissue sections and cell preparations. Various clones, which are commercially available to detect the same antigen, are listed and the sensitivities and specificities of the antibodies are discussed. Importantly, the authors, all pathologists with a wealth of experience in immunostaining procedures, provide fully referenced details and expert advice on each reagent, which the reader will find invaluable. Finally, the appendices provide easily accessible and clear summaries of selected antibody panels for specific diagnostic situations, details of heat-induced antigen/epitope retrieval (including the use of microwaves) and a useful reference to the websites of the main antibody suppliers.

The epic history of how antibiotics were born, saving millions of lives and creating a vast new industry known as Big Pharma. As late as the 1930s, virtually no drug intended for sickness did any good; doctors could set bones, deliver babies, and offer palliative care. That all changed in less than a generation with the discovery and development of a new category of medicine known as antibiotics. By 1955, the age-old evolutionary relationship between humans and microbes had been transformed, trivializing once-deadly infections. William Rosen captures this revolution with all its false starts, lucky surprises, and eccentric characters. He explains why, given the complex nature of bacteria-and their ability to rapidly evolve into new forms-the only way to locate and test potential antibiotic strains is by large-scale, systematic, trial-and-error experimentation. Organizing that research needs large, well-funded organizations and businesses, and so our entire scientific-industrial complex, built around the pharmaceutical company, was born. Timely, engrossing, and eye-opening, Miracle Cure is a must-read science narrative-a drama of enormous range, combining science, technology, politics, and economics to illuminate the reasons behind one of the most dramatic changes in humanity's relationship with nature since the invention of agriculture ten thousand years ago.

Lab reports are used across a range of subjects, and they require very different skills to writing essays or literature reviews. Get the know-how you need to avoid losing marks and write your report with ease. Understand the structure so you know what's different before you start Avoid wasting time with insider tips on style and content Check your final report so you submit your best work. Super Quick Skills provide the essential building blocks you need to succeed at university - fast. Packed with practical, positive advice on core academic and life skills, you'll discover focused tips and strategies to use straight away. Whether it's writing great essays, understanding referencing or managing your wellbeing, find out how to build good habits and progress your skills throughout your studies. Learn core skills quickly Apply right away and see results Succeed in your studies and life. Super Quick Skills give you the foundations you need to confidently navigate the ups and downs of university life.

The United States Environmental Protection Agency (EPA) is responsible for assessing the extent of environmental contamination and human health consequences in the event of a radiological incident such as a terrorist incident involving radioactive materials. Although the EPA will be mainly involved in the intermediate and recovery phases of an incident response, there also may be involvement in some activities in the early phase. This book describes project method validation guidance that a radioanalytical laboratory should comply with in order to validate methods used to process samples submitted during a radiological or nuclear incident, such as that caused by a terrorist attack. These responsibilities include response and recovery actions to detect and identify radioactive substances, and to co-ordinate federal radiological monitoring and assessment activities.

Dieses Lehrbuch bietet Antworten auf Fragen wie "Wie funktioniert diese mikrobiologische Methode ganz praktisch? Wie kann ich meine Forschung um eine sinnvolle Methode erweitern?" Vergleichbar einem Kochbuch sind Zutaten und einzelne Handgriffe ubersichtlich aufgelistet und mit praktischen Tipps und Gefahrenhinweisen versehen. Ob zu Fluoreszenzmikroskopie, Genomik oder Isolierung und Kultivierung von Bakterien, Archaeen und Pilzen - erfahrene MikrobiologInnen finden hier ebenso schnelle Hinweise wie Quereinsteiger, die ihr Arbeitsgebiet mikrobiologisch erweitern wollen. Die fachkundigen PraktikerInnen Astrid Brandis-Heep, Erika Kothe und Timo Zimmermann haben ubersichtlich praktische Methoden und Ratschlage fur den Laboralltag zusammengestellt, die - mikrobiologisches Grundwissen vorausgesetzt - Masterstudierenden, Doktoranden, Postdocs und fortgeschrittenen Arbeitsgruppen sowohl im Alltag als auch fur eine Neuausrichtung Ihrer Forschung entscheidende Hinweise geben.

This book presents, in two parts, a review concerning the use of Rattus norvegicus as a model for the study of neurological disorders and oncological diseases (mammary cancer). In the first part, the neurological disorders are addressed in important neurological diseases, such as: Autism, Parkinsons, Huntingtons and Alzheimers disease, and two neuromotor dysfunctions (amyotrophic lateral sclerosis and ataxia). The second part reveals the importance of the rat as a model for the study of mammary cancer, presenting the diversity of rat strains and models available for the study of this oncological disease. This part is mainly focused on the chemically-induced models of mammary cancer and on the importance of the rat for the development of new prophylactic and therapeutic strategies to fight this disease.For many years and up to the present, rat models have allowed the study of innumerable diseases, as well as their etiology, pathogenesis, evolution and treatment. Due to this, the role of this species for the evolution of medicine is undoubtable.Since the information presented in this book was obtained from years of works of expertise in the field, we hope that it be useful for those researchers aiming to use Rattus norvegicus as a model to study human diseases, providing them with valuable information for the adequate selection and management of this species in their experiments.

This book is part of a series of titles that are a spin-off of the Shingo Prize-winning book Leveraging Lean in Healthcare: Transforming Your Enterprise into a High Quality Patient Care Delivery System. Each book in the series focuses on a specific aspect of healthcare that has demonstrated significant process and quality improvements after a Lean implementation. Lean principles can help medical laboratories drive up efficiencies and quality without increasing costs or compromising quality. Leveraging Lean in Medical Laboratories: Creating a Cost Effective, Standardized, High Quality, Patient-Focused Operation provides a functional understanding of Lean laboratory processes and quality improvement techniques. This book is an ideal guide for healthcare executives, leaders, process improvement team members, and inquisitive frontline workers who want to implement and leverage Lean in medical laboratories. Supplying detailed descriptions of Lean tools and methodologies, it identifies powerful Lean solutions specific to the needs of the medical laboratory. The first section provides an overview of Lean concepts, tools, methodologies, and applications. The second section focuses on the application of Lean in the laboratory environment. Presenting numerous examples, stories, case studies, and lessons learned, it examines the normal operation of each area in the lab environment and highlights the areas where typical problems occur. Next, it walks readers through various Lean initiatives and demonstrates how Lean tools and concepts have been used to achieve lasting improvements to processes and quality of care. It also supplies actionable blueprints that readers can duplicate or modify for use in their own institutions. Illustrating leadership's role in achieving departmental goals, this book will provide you with a well-rounded understanding of how Lean can be applied to achieve significant improvements throughout the entire continuum of care.

"In Situ Molecular Pathology and Co-Expression Analyses" explains, in easy-to-understand language, simplified waysof understanding and performing in situ hybridization and immunohistochemistry tests. The book also focuses on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses.

The fields of in situ hybridization and immunohistochemistry have expanded rapidly due to the use of computer-based analysis. To get the most out of these automated platforms, researchers and diagnostic biomedical investigators must have a solid understanding of the basics of in situ-based tests, protocols, and regimens for troubleshooting.

Practicing molecular pathologists, clinical chemists, and toxicologists, as well as clinicians and researchers in training, will benefit from this book's clear presentation of protocols and theoretical framework.
Includes over 200 easy-to-follow experimental protocolsFeatures chapter-ending summaries of "Key Points to Remember" to bring beginners up to speed with any seasoned veteran in the fieldOffers two chapters written by industry leaders in the fields of in situ hybridization, immunohistochemistry, and computer software for co-expression analyses"

This text discusses the applications, outcomes, and controversies of using animal models in cancer research and human disease. The topics include murine models of sporadic and inflammation-related colon carcinogenesis with particular focus on the AOM/DSS chemically induced colorectal cancer model; large animal models of human cardiovascular disease; developments of cell therapeutic strategies for Alzheimer's disease using animal models; the techniques and limitations of murine models of human colorectal cancer; animal models for antineoplastic chemotherapy-induced alopecia; and development of columnar-lined oesophagus with or without goblet cells in rate duodengastroesophageal reflux models through gut regenerative cell lineage (GRCL).

Defined as red blood cell break down and the release of hemoglobin and intracellular contents into the plasma, haemolysis can seriously impact patient care as well as the laboratory's reputation through its affect on test results. Therefore, the European Preanalytical Scientific Committee, in collaboration with the International Federation of Clinical Chemistry Working Group on Patient Safety, have designed a questionnaire to collect data on prevalence and management of haemolytic specimens referred to the clinical laboratories for clinical chemistry testing. The new book will help identify the areas where haemolysis occurs most frequently, which can, in turn, guide further analysis about why it is occurring. Once these elements are known, practices and procedures can be implemented to dramatically reduce haemolysis and avoid erroneous laboratory results affecting patient care and increasing laboratory costs.

Changes in the organization of health services in developing countries have led to the district level assuming more responsibility for the planning, delivery and quality of community health care. This fully up-dated new edition has been produced to help those working in the district laboratory, and those responsible for the organization and management of community laboratory services and the training of district laboratory personnel. Replacing the previous publication Medical Laboratory Manual for Tropical Countries, this book provides an up-to-date practical bench manual, taking a modern approach to the provision of a quality medical laboratory service. It includes practical accounts of: organization and staffing of district laboratory services; total quality management; health and safety; equipping district laboratories; parasitological tests, illustrated in colour; clinical chemistry tests; how to plan a training curriculum for district laboratory personnel. Volume 2, published in late 1999, covers microbiological tests, haematological tests and blood transfusion tests.

This is the first book in a series that serves the dual purpose of preparing
students of CLS programs for certification exams and giving cross training
skills to established clinical lab scientists who now find themselves called
on to work in other areas of the lab. This book uses a building block
approach to convey the material in a logical fashion. Starting with an
overview of medicine and the medical laboratorys relationship, the text
proceeds to the diagnostic value of those analytes normally associated with
clinical chemistry, on to technologies of analysis, quality assurance, and
statistical analysis of laboratory data. A&L Outline Review of Clinical Chemistry
makes it easy to optimize your study sessions and
use your time efficiently, and helps you quickly locate key concepts. This
PREP guide provides pretests to help you identify areas for extra attention,
and gives you what you need to know in an easy-to-remember format, packed
with study-enhancing devices such as illustrations and learning objectives.

A growing body of medical research suggests that many medical tests used for diagnosis, prognosis, and monitoring response to treatment are unnecessary or inappropriate, yield inaccurate or misleading results, and add to the incidence of iatrogenic disease. Many such tests go into common clinical use without convincing documentation of their value because current methods for evaluating medical tests produce inconsistent results. Evaluating Medical Tests presents a systematic, objective methodology by which to determine the effectiveness of medical tests. Kraemer clearly and concisely shows how to define statistical terms and approaches consistently from study to study, how to stipulate statistical assumptions underlying various approaches, how to check for empirical validity, and how to judge the robustness of statistical outcomes, resulting in models that integrate many different approaches and extend the strengths of each. Statisticians, evaluators, researchers, practitioners, policymakers, and advanced students concerned with the evaluation of medical tests used to make clinical decisions for patient care and for research will find this essential, thought-provoking reading. "This volume is a pleasure to read. It succinctly combines carefully reasoned conceptualization with the mathematical methods for addressing test evaluation problems. With clinically relevant, illustrative examples, it draws on the author's extensive experience and research in biomedical statistics. It should be required reading for medical students and practitioners. It is invaluable to researchers and statisticians, and it provides a logical, scientific basis for efforts at medical cost containment. It is a classic! --from the Foreword by A. John Rush, M.D. "This fascinating book integrates a wide range of statistical and methodological concepts, reflects a thorough introduction to the issues confronted by evaluators of [medical] tests, and challenges motivated readers to adopt these procedures in their own research. This is an exciting book, with obvious strong policy implications concerning the way that [medical] tests are evaluated and the need for standards in this regard. . . . Evaluating Medical Tests should be of interest to all professionals who depend on tests as an adjunct in the decision-making process." --Paul R. Yarnold Northwestern University Medical School "Dr. Kraemer has undertaken a most massive and laudable task in writing Evaluating Medical Tests. . . . Dr. Kraemer's book stands as a flagship for those concerned to understand the reasons behind our escalating health care costs and the implications this holds for every American. . . . This leads us to one of the innovative features of Dr. Kraemer's book: 'This is not merely a statistical issue, or merely a medical issue, it is a national policy issue of the greatest importance.'. . . She does not assume that her readers are either statisticians or physicians. As such, she skillfully guides one through the terrain of medical and statistical jargon and points our where the assumptions of the statistics are either not stipulated or incongruent with the medical test and data being evaluated, thus rendering results and interpretations suspect. Another plus of Dr. Kraemer's writing is her willingness to use '. . . the first person singular to differentiate . . . comments from presentations of what I would consider provable or documentable facts. . . ' In so doing, she raised issues and calls medical professionals, policymakers, and patients into dialogue to consider the impact of the use of these various techniques in diagnosis. . . . Evaluating Medical Tests is of specific relevance to rehabilitation counselors who proceed with their clients through the maze of diagnostic and prognostic tests. . . . It is especially important for rehabilitation counselors who act as expert witnesses to have a working knowledge of this material to enhance their discussions with health care personnel." --Journal of Applied Rehabilitation Counseling

Comprehensive Biomarker Discovery and Validation for Clinical Application provides the reader with an extensive introduction into all aspects of proteomics biomarker discovery, validation and development. It discusses the current status of science and technology, its limitations, bottlenecks as well as future development trends to improve the success rate of translating biomarker discovery into useful clinical tests. The most important feature of the book is to provide an overview of current technologies and the challenges encountered during biomarker discovery and validation, such as patient selection, sample handling, data processing, statistical analysis and registration and approval of validated biomarkers through European and US regulatory authorities. The authors introduce the reader to each of these topics in significant detail and provide examples or guidelines for best practice. There are prominent chapters included on biomarkers in translational and personalised medicine; an introduction to regulatory affairs and bring biomarkers to the market; biomarker discovery and the use of mass spectrometry based profiling platforms; MALDI imaging techniques in tissue-based biomarkers discovery and a clinical application study on the use of diagnostic assays for early diagnosis of heart failure using various proteomic methods. The book concludes with a final chapter on future trends in biomarker discovery and validation. The book targets a readership of industrial and academic researchers that are involved in biomarker discovery and validation or that manage biobanks, develop sample preparation methods, analytical profiling systems and bioinformatics tools. Common pitfalls and success stories in biomarker discovery are highlighted and guidelines for best practice are provided for the different parts of the procedure. The book will be an essential information resource for scientists working in the field.