Blood transfusion is considered a life-saving therapy since ancient times, but, at the same time, a high-risk procedure. Nowadays the common perception is that infection is the greatest risk, even if the blood has never been safer from this point of view. Currently, the residual risk of transfusion must be related mainly to immunological mechanisms underlying to AB0 and minor blood systems, to compatibility of blood transfused and to development of irregular antibodies in transfused patients. "Transfusion Medicine and Patient Safety" aims to provide the basic of immunohematology to readers and to analyze the transfusional process highlighting the most critical points, thus more exposed to errors. Screening on blood and blood components for infectious diseases along with the surveillance action on emerging viruses results in the drastic reduction of post-transfusion infection, together with the potential to further increase the level of security from infection through the inactivation of blood components. The text also describes the major diagnostic systems and organizational models that modern technology provides us with a correct immunohematological diagnosis and an appropriate trasfusional therapy.

This book describes various methods of decontamination and how the methods work. There is a discussion of the various cleaning and disinfection methods utilized, along with details of how to qualify these methods. It also describes new technologies that may be useful in the battle for decontamination across industries. Finally, this book provides a single resource on how one can address contamination issues for a variety of manufacturing processes and industries. Explores new technologies that may be useful in the battle for decontamination Examines various methods of decontamination and how the methods work Addresses contamination issues for a variety of manufacturing processes and industries Describes how to detect contaminants as well as how to deal with contaminants that are present Includes methods for both decontamination (reaction) and preventing contamination (proactive)

One of the most notable trends in biomedical science in recent years has been the increasing use of molecular techniques as part of the diagnosis of disease. As such, there is a growing need for students to understand the technological basis of molecular analysis and to have a comprehensive appreciation of their use in diagnosis. Combining coverage of molecular techniques with their application to diagnostic pathology, this book provides students with a thorough and up to date appreciation of the scope of molecular analysis, the principles of the technology used, and thematic diagnostic application. As part of the Fundamentals of Biomedical Science series, the book provides a unique blend of theory and practice, featuring a range of learning features to help students assimilate the information presented quickly and effectively. It will be relevant to undergraduate students on a wide variety of biomedical pathways, cutting across traditional discipline boundaries to provide a unified overview of molecular diagnostics.

A complete examination of the uses of the atomic force microscope in biology and medicine

This cutting-edge text, written by a team of leading experts, is the first detailed examination of the latest, most powerful scanning probe microscope, the atomic force microscope (AFM). Using the AFM, in combination with conventional tools and techniques, readers gain a profound understanding of the cell, subcellular organelles, and biomolecular structure and function.
The text begins with three chapters describing the molecular machinery and mechanism of cell secretion and membrane fusion in cells, using approaches that combine AFM, electron microscopy, X-ray diffraction, photon correlation spectroscopy, molecular biology, biochemistry, and electrophysiology. The discovery of a new cellular structure the "porosome" or fusion pore--the cells secretory machinery, the molecular mechanism of membrane fusion in cells, and the expulsion of intravesicular contents during cell secretion are outlined in the first three chapters. The book also covers:
* Identification of the "porosome" in the growth hormone secreting cell of the pituitary gland
* Probing the structural and physical properties of microbial cell surfaces
* Scanning probe microscopic characterization of the higher plant cell wall and its components
* Case studies of nano drug delivery systems using engineered dendrimers
* AFM techniques for studying living cells
* Investigating the intermolecular forces of leukocyte adhesion molecules
* Protein-protein interactions
* Micromechanical properties of lipid bilayers and vesicles
The text concludes with four chapters that examine new and emerging approaches in the use of force microscopy in biology and medicine.
This text is ideal for advanced undergraduate and graduate students and researchers in cell and molecular biology, genetics, genomics, physiology, neuroscience, biophysics, and biochemistry. Not only does it provide the theory, but also practical considerations such as the selection of the right tools and approach.

The Oxford Handbook of Clinical and Healthcare Research is an evidence-based, succinct, and easy-to-use reference for the full range of clinical and healthcare research topics. Providing a wide breadth of essential knowledge, this comprehensive text takes the researcher through the steps from general good clinical practice in healthcare research to the process and management of research. This handbook includes clear instructions on the legislative and practical requirements of commissioning, conducting, analysing, and reporting research for those in clinical or healthcare practice, education, or training. Written with Good Clinical Practice (GCP) education in mind, it includes valuable information needed for the accredited certificates and diploma-level benchmark exams now commonly required by employers. This is a definitive text for all clinical and healthcare research students, as well as graduates with an interest in clinical and healthcare research.

Emphasizing the relevance of microbiology to a career in the health professions, Burton's Microbiology for the Health Sciences provides the vital microbiology information you need to protect yourself and your patients from infectious diseases.

Specifically intended for lab-based biomedical researchers, this practical guide shows how to design experiments that are reproducible, with low bias, high precision, and widely applicable results. With specific examples from research using both cell cultures and model organisms, it explores key ideas in experimental design, assesses common designs, and shows how to plan a successful experiment. It demonstrates how to control biological and technical factors that can introduce bias or add noise, and covers rarely discussed topics such as graphical data exploration, choosing outcome variables, data quality control checks, and data pre-processing. It also shows how to use R for analysis, and is designed for those with no prior experience. An accompanying website (https://stanlazic.github.io/EDLB.html) includes all R code, data sets, and the labstats R package. This is an ideal guide for anyone conducting lab-based biological research, from students to principle investigators working in either academia or industry.

FLOW CYTOMETRY PRINCIPLES FOR CLINICAL LABORATORY PRACTICE
Quality Assurance for Quantitative Immunophenotyping
Marilyn A. Owens and Michael R. Loken
Flow Cytometry Principles for Clinical Laboratory Practice is an invaluable benchtop reference and introduction for first-time users of flow cytometry in clinical diagnostic laboratories. This manual provides authoritative coverage of accepted methods, quality controls, and standards for the cytometric analysis of clinical samples with particular relevance to CD4+ lymphocytes. CD4+ lymphocyte determination by immunophenotyping is the standard method for evaluating the status of HIV infection, and the single most frequent application of flow cytometry in a diagnostic setting.
Flow Cytometry Principles for Clinical Laboratory Practice will appeal to clinical laboratory technologists, medical practitioners, and cytometry lab directors in their role as instructors of new personnel. In addition, it is a valuable resource for clinical and biomedical researchers, establishing quantitative cytometric systems for studies in immunology and hematology.

For more than 65 years, this best-selling text by Drs. Barbara J. Bain, Imelda Bates, and Mike A. Laffan has been the worldwide standard in laboratory haematology. The 12th Edition of Dacie and Lewis Practical Haematology continues the tradition of excellence with thorough coverage of all of the techniques used in the investigation of patients with blood disorders, including the latest technologies as well as traditional manual methods of measurement. You'll find expert discussions of the principles of each test, possible causes of error, and the interpretation and clinical significance of the findings. A unique section on haematology in under-resourced laboratories. Ideal as a laboratory reference or as a comprehensive exam study tool. Expert ConsultT eBook version included with purchase. This enhanced eBook experience allows you to search all of the text, figures, and references from the book on a variety of devices. diagnosis, molecular testing, blood transfusion- and much more. Complete coverage of the latest advances in the field. An expanded section on coagulation now covers testing for new anticoagulants and includes clinical applications of the tests.

Das vorliegende Werk gibt einen aktuellen UEberblick uber die wichtigsten POCT-Analyseverfahren und POCT-Gerate sowie deren klinische Anwendung. Daruber hinaus liefert es Informationen, u.a. zu medizinischer und wirtschaftlicher Bedeutung von POCT, Methodik, Organisation, Qualitatssicherung und den Trends fur die Zukunft. Die 3. Auflage wurde komplett aktualisiert und erweitert und tragt den neuesten Entwicklungen auf dem Gebiet Rechnung. Neue Themen sind u.a. POCT-taugliche molekularbiologische Verfahren und der internationale Vergleich der Qualitatsregeln fur POCT. Das Buch wendet sich an alle, die bereits patientennahe Laboruntersuchungen durchfuhren oder zukunftig einfuhren moechten: AErzte aller Fachbereiche, POCT-Beauftragte, Verantwortungstrager in Kliniken und Krankenhausverwaltungen. Aber auch die Entwicklungsabteilungen von IVD-Unternehmen erhalten wertvolle Informationen uber Entwicklungstendenzen und die neuen Anforderungen der europaischen Gesetzgebung an POCT-Gerate und -Reagenzien.

Specifically intended for lab-based biomedical researchers, this practical guide shows how to design experiments that are reproducible, with low bias, high precision, and widely applicable results. With specific examples from research using both cell cultures and model organisms, it explores key ideas in experimental design, assesses common designs, and shows how to plan a successful experiment. It demonstrates how to control biological and technical factors that can introduce bias or add noise, and covers rarely discussed topics such as graphical data exploration, choosing outcome variables, data quality control checks, and data pre-processing. It also shows how to use R for analysis, and is designed for those with no prior experience. An accompanying website (https://stanlazic.github.io/EDLB.html) includes all R code, data sets, and the labstats R package. This is an ideal guide for anyone conducting lab-based biological research, from students to principle investigators working in either academia or industry.

Dieser Atlas beschreibt in einheitlich strukturierter Form alle in der Gynakologie und Gebutshilfe wichtigen Infektionen. Bei jedem Erreger wird von der Epidemiologie und Transmission, der Biologie, der Infektabwehr und Pathogenese zum klinischen Bild, der Diagnostik, der Therapie und der Pravention, sowie der Besonderheiten in der Gynakologie und bei Schwangeren alles dargestellt und mit Abbildungen belegt. Besonderen Wert wird auf die Therapie HIV-infizierter Schwangerer gelegt. Zu einigen Keimen wurden leicht verstandliche cartoonartige Abbildungen von den Herausgebern gezeichnet.

This series is directed to healthcare professionals who are leading the tra- formation of health care by using information and knowledge. Launched in 1988 as Computers in Health Care, the series offers a broad range of titles: some addressed to specific professions such as nursing, medicine, and health administration; others to special areas of practice such as trauma and radi- ogy. Still other books in the series focus on interdisciplinary issues, such as the computer-based patient record, electronic health records, and networked healthcare systems. Renamed Health Informatics in 1998 to reflect the rapid evolution in the discipline now known as health informatics, the series will continue to add titles that contribute to the evolution of the field. In the series, eminent - perts, serving as editors or authors, offer their accounts of innovations in health informatics. Increasingly, these accounts go beyond hardware and so- ware to address the role of information in influencing the transformation of healthcare delivery systems around the world. The series also increasingly focuses on "peopleware" and the organizational, behavioral, and societal changes that accompany the diffusion of information technology in health services environments.

La questione della sicurezza dei pazienti e del rischio clinico rappresenta da sempre un problema in medicina, ma e a partire dagli ultimi anni che essa e diventata un ambito prioritario della qualita nei servizi sanitari. La medicina non e una scienza esatta e le cure mediche non sono sempre efficaci e affidabili. La materia e inoltre cosi vasta e complessa da rendere impossibile agli operatori una conoscenza completa di ogni aspetto; a cio si aggiunge il fatto che i pazienti non sempre si attengono correttamente alle indicazioni di terapia. La valutazione del rischio e l'analisi degli eventi avversi possono quindi contribuire ad accrescere i livelli di sicurezza degli assistiti, a ridurre l'inappropriatezza delle procedure e a impiegare meglio le risorse umane e tecnologiche. Questo volume, dopo una prima valutazione dello stato dell'arte della sicurezza del paziente in Italia e all'estero, presenta i metodi piu diffusi per l'analisi degli eventi avversi nelle diverse specialita (medicina d'urgenza, ostetricia e ginecologia, oncologia, salute mentale, ecc.) e nei servizi di supporto (laboratori analisi, radiologia, trasfusioni, farmaceutica). Sono inoltre esaminati gli incidenti piu frequenti in strutture extraospedaliere (come ambulatori di medicina generale, servizi sanitari delle carceri). Quest'opera, caratterizzata da una particolare vastita di argomenti trattati, descrive come contenere il rischio e prevenire gli eventi avversi in sanita, analizzando la natura dell'errore umano e applicando le pratiche di sicurezza piu efficaci.

Die Leitlinie gibt praktische und grundlegende Empfehlungen zur (labormedizinischen) Diagnostik von Virusinfektionen bei Schwangeren, Foten und Neugeborenen. Sie richtet sich an alle Arzte, die Schwangere betreuen und behandeln, daruber hinaus an Perinatologen, Neonatologen und Allgemeinmediziner sowie an Virologen, Labormediziner und Arbeitsmediziner.

Bei der Zusammenstellung der Daten wurden diejenigen Virusinfektionen berucksichtigt, die erwiesenermassen die Gesundheit des werdenden Kindes oder der Mutter gefahrden konnen.

Die Leitlinie gliedert sich in einenallgemeinen Uberblick und allgemeine Empfehlungen, Empfehlungen zu impfpraventablen Infektionen und Empfehlungen zu nicht impfpraventablen Infektionen.

Im Anhang finden sich HInweise auf bereits erschienene Leitlinien zu verwandten Themen aus Deutschland, Schweiz und USA."

Die Forschung und Anwendungsentwicklung in dem Bereich chemischer und biochemischer Sensoren ist weiterhin in einem schnellen Wachstum begriffen. Die Erfahrungen des letzten Jahrzehnts haben jedoch gezeigt, dass die erfolgreiche Entwicklung solcher Sensoren, die auch den harten Routinebedingungen in den vielfaltigen Anwendungsgebieten widerstehen, nur dann moglich ist, wenn Chemiker und Ingenieure kooperieren. Daher ist es das Ziel dieses Lehrbuches, sowohl Chemikern als auch Ingenieuren, Lebensmittel- und Biotechnologenin einer streng systematischen aber sehr praxisorientierten Darstellung die Technologie und die Anwendung chemischer Sensoren nahezubringen.

Der interdisziplinare Ansatz uberbruckt die unterschiedlichen Denkweisen in Chemie, Physik und Ingenieurwissenschaften erfolgreich."

The Oxford Textbook of Medical Mycology is a comprehensive reference text which brings together the science and medicine of human fungal disease. Written by a leading group of international authors to bring a global expertise, it is divided into sections that deal with the principles of mycology, the organisms, a systems-based approach to management, fungal disease in specific patient groups, diagnosis, and treatment. The detailed clinical chapters take account of recent international guidelines on the management of fungal disease. With chapters covering recent developments in taxonomy, fungal genetics and other "omics", epidemiology, pathogenesis, and immunology, this textbook is well suited to aid both scientists and clinicians. The extensive illustrations, tables, and in-depth coverage of topics, including discussion of the non-infective aspects of allergic and toxin mediated fungal disease, are designed to aid the understanding of mechanisms and pathology, and extend the usual approach to fungal disease. This textbook is essential reading for microbiologists, research scientists, infectious diseases clinicians, respiratory physicians, and those managing immunocompromised patients. Part of the Oxford Textbook in Infectious Disease and Microbiology series, it is also a useful companion text for students and trainees looking to supplement mycology courses and microbiology training.

Wle schon mein Buch "Diagnostische Bewertungvon Laborbefunden" so ist auch dieses aus der Praxis heraus fur die Praxis entstanden. Die in der taglichen Laborarbeit auftretenden Fragestellungen sollen rasch beantwortetwerden konnen. Zwar gibt es Lehrbiicher und andere Fach- literatur, die zum ersten Lernen und zur Erweiterung der Kenntnisse hervorragend geeignet sind; in der taglichen Arbeit geben sie jedoch nicht rasch und ausfuhrlich genug Auskunft, insbesondere wenn es urn die Frage geht, welche Untersuchungsmethode gewahlt werden sollte. Von den Symptomen ausgehend, sollten zunachst nur Unter- suchungsprogramme erarbeitet werden, die die Differentialdiagnose wirksam unterstiitzen konnen. Da jedoch in der taglichen Praxis immer wieder Fragen nach bestimmten Krankheitsbildern auftreten, muEten nicht nur Symptome, sondern auch die wichtigsten Krankheiten aufge- nommen werden. Zur leichteren Auffindbarkeit sind daher die Symp- tome alphabetisch neben den Krankheiten aufgefiihrt. Da das Buch auf Teilfragen aus dem Gesamtgebiet der Medizin Antwort gibt, bezieht sich auch die Darstellung vorwiegend auf solehe T eilbereiche. Dadurch ist das Buch umfangreicher geworden als zu- nachst vorgesehen. Denn es ist zum Beispiel schwierig, beim Thema "Ulcus ventriculi" nur begleitende und im Verhaltnis zur Hauptdia- gnostik verhaltnismaEig unwichtige Laborbefunde allein darzustellen; vielmehr ist es erforderlich, bei bestimmten Krankheitsbildern auch auf andere technische Untersuchungen hinzuweisen.

The traditional concept of biological sensors, based on enzymatic receptors and potentiometric or amperometric transducers has undergone several genera tions of development. Such types of biosensors have been extensively reviewed, described in many textbooks and commercialized. This book is focused on alternative types of chemical and biological sensors or sensor-like structures and approaches, exploring electrical or electrochemical signal detection. Spe cial attention is paid to applications of linear and nonlinear impedance. Some basic ideas in this field are very old - first described, for example, in the classi cal work by Warburg at the end of the 19th century. Later impedance spectrosco py became a popular approach for studying adsorption of organic molecules on polarizable metal electrodes. However, analytical applications of this approach have only been developed over the last decade, after the establishment of the technology of self-assembled monolayers. In that time, when many scientists were disappointed with attempts to use the Langmuir-Blodgett technique for manufacturing electrochemical devices, the self-assembled monolayers became a viable technology for immobilization of organic molecules on electrodes and for the formation of covalently stabilized receptor layers and even more sophis ticated organic nano- and microstructures. This resulted in the development of numerous analytical applications of impedometric methods which are the main topic of the present book. The book consists of four parts."

DNA Methods in Clinical Microbiology describes the novel DNA-based technology now used in the diagnosis and management of infectious diseases. It is a concise, yet readable, overview written primarily for clinicians, clinical microbiologists, medical students and undergraduates in medical and veterinary microbiology. The book has two primary aims. First, to explain the principles of these methods at the molecular' level. Second, to provide a clinical perspective by reporting results from actual DNA-based investigations on a range of specimens. Those approaching DNA methods for the first time are assisted by a brief resume of the relevant features of nucleic acids (Chapter 2): this information is essential for an understanding of later chapters. Subsequent text covers detection, characterization and quantification of pathogens by a variety of methods - e.g., target amplification (PCR, LCR, NASBA, TMA and SDA), signal amplification (bDNA) and probe-based techniques; the chapter on typing describes nearly twenty named molecular methods, including spoligotyping and MLST. All chapters include an adequate range of current reference from which, if required, detailed protocols can be obtained. The diagrams are clear, and readers are assisted by a detailed index."

In un mondo in continua evoluzione tecnologica e vitale seguire un metodo che aiuti a tenere sotto controllo l'adeguatezza del prodotto alle aspettative del mercato, alle esigenze del cliente e ai requisiti di legge, senza appesantire gli sviluppi o le produzioni e fornendo le adeguate garanzie.

Lo scopo di questo testo e fornire al lettore una visione generale della qualita applicata al settore delle scienze della vita, facendo riferimento sia alla normativa in vigore per la progettazione di un sistema di gestione per la qualita, sia ai principali strumenti per la pianificazione delle attivita, per l'organizzazione, elaborazione e analisi dei dati, secondo un metodo rigoroso e corretto.

Approfondimenti tematici sono affidati a esperti di realta di prestigio (MolMed, LaboRaf, Bayer Healthcare, Areta International) e integrati da esempi di applicazione nei laboratori diagnostici, nella ricerca scientifica e nella sperimentazione clinica, con particolare riferimento alla produzione di massa e di nicchia in asepsi.

Il volume rappresentera

• una guida preziosa per il manager, che intende porre particolare attenzione al ritorno degli investimenti in qualita
• un utile testo di riferimento per gli operatori del settore e i ricercatori
• un valido strumento di formazione per gli studenti dei corsi di laurea in Biotecnologie, Chimica e Tecnologia Farmaceutica, Farmacia, Biologia, Tecniche di Laboratorio Biomedico.

Selecting the best embryo to transfer to the uterus is key to successful in vitro fertilization (IVF). A huge amount of research has been devoted to this topic and there are numerous methods used, from simple morphological assessment to molecular biological techniques to assess the genome and metabolism of the newly fertilized embryo. For many of these techniques, an adequate evidence base is lacking, and expert opinion is valuable. Clinical imperatives require ranking all embryos in a cohort according to their viability, thereby enabling the selection of the best embryo to optimize live birth outcome: a key indicator used to measure and rate IVF Clinics worldwide. This clear and informative manual will provide embryologists and clinicians with an overview of the tools now available to assist in embryo selection, as well as evidence for their efficacy and safety and the broader considerations that must underlie these important clinical decisions.

Choosing wisely for better patient care. Lab Literacy for Doctors helps you make quick and efficient decisions about the right tests for typical clinical situations, thereby improving patient care. In a study published by the Journal of the American Board of Family Medicine (2014-03-01), primary care physicians reported that 15% of the time they are unsure about ordering lab tests and 8% of the time they are unsure about interpreting the results. This raises concerns about the need for more efficient and cost-effective lab test utilization. Lab Literacy for Doctors addresses these concerns in a practical, up-to-date, and easy-to-use format. Refer to this essential guide for: Diagnostic algorithms. A quick index summarizing the clinical utility of common tests. Advice and information on lab errors, false positives and negatives, and blood and tissue collection. Sections on dermatology, ENT and respiratory system, endocrine system, fatigue, GI and hepatic system, gynecology and pregnancy, routine screening, and more.