This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Pattern Recognition Receptors: Methods and Protocols aims to ensure successful results in the further study of this vital field.

This edited volume focuses on the characterization, reclamation, bioremediation, and phytoremediation of salt affected soils and waterlogged sodic soils. Innovative technologies in managing marginal salt affected lands merit immediate attention in the light of climate change and its impact on crop productivity and environment. The decision-making process related to reclamation and management of vast areas of salt affected soils encompasses consideration of economic viability, environmental sustainability, and social acceptability of different approaches. The chapters in this book highlight the significant environmental and social impacts of different ameliorative techniques used to manage salt affected soils. Readers will discover new knowledge on the distribution, reactions, changes in bio-chemical properties and microbial ecology of salt affected soils through case studies exploring Indian soils. The contributions presented by experts shed new light on techniques such as the restoration of degraded lands by growing halophyte plant species, diversification of crops and introduction of microbes for remediation of salt infested soils, and the use of fluorescent pseudomonads for enhancing crop yields.

r ed Algae in Genome Age book most people reading this book have childhood memories about being enthralled at the beach with those rare and mysterious living forms we knew as seaweeds. We were fascinated at that time by their range of red hues and textures, and most of all, their exotic beauty. t o a scientist, red algae represent much more than apparent features. t heir complex forms have attracted morphologists for centuries; their intricate life cycles have brought more than one surprise to plant biologists familiar only with ferns and fowering plants; their unusual tastes have been appreciated for mill- nia, and their valuable chemical constituents have been exploited for nearly as long, most recently by biotech companies; their diversity in marine, freshwater, and t- restrial environments has offered centuries of engaging entertainment for botanists eager to arrange them in orderly classifcation systems; still, the red algae continue to teach us how many more challenges need to be overcome in order to understand their biodiversity, biological functions, and evolutionary histories.

Plants live in a constantly changing environment from which they cannot physically escape. Plants therefore need signalling and response mechanisms to adapt to new local conditions. The ef?cacy of such mechanisms underlies the plant performance during stress and therefore also impacts greatly on agricultural productivity. M- ulation of ion channel activity not only provides a means for rapid signal generation 2+ but also allows adjustment of cellular physiology. For example, Ca permeable ion 2+ channels can transduce environmental stimuli into Ca -encoded messages which can modify the gene expression. Furthermore, ion channel activity is essential to control cellular ion homeostasis that impacts on plant responses to drought, salinity, pathogens, nutrient de?ciency, heavy metals, xenobiotics and other stresses. This volume focuses on the crucial roles of different types of ion channel in plant stress responses. Functions of ion channels are discussed in the context of mechanisms to relay external and endogenous signals during stress and as mechanisms to regulate cellular ion homeostasis and enzymatic activities in the context of biotic and abiotic stress. The chapters presented cover cation and anion channels located in various cellular compartments and tissues.

The entire range of the developmental processes in plants is regulated by a shift in the hormonal concentration, tissue sensitivity and their interaction with the factors operating around them. Out of the recognized hormones, attention has largely been focused on five - Auxins, Gibberellins, Cytokinin, Abscisic acid and Ethylene. However, the information about the most recent group of phytohormone (Brassinosteroids) has been incorporated in this book. This volume includes a selection of newly written, integrated, illustrated reviews describing our knowledge of Brassinosteroids and aims to describe them at the present time. Various chapters incorporate both theoretical and practical aspects and may serve as baseline information for future researches through which significant developments are possible. This book will be useful to the students, teachers and researchers, both in universities and research institutes, especially in relation to biological and agricultural sciences.

Precise regulation of gene expression in both time and space is vital to plant growth, development and adaptation to biotic and abiotic stress conditions. This is achieved by multiple mechanisms, with perhaps the most important control being exerted at the level of transcription. However, with the recent discovery of microRNAs another ubiquitous mode of gene regulation that occurs at the post-transcriptional level has been identified. MicroRNAs can silence gene expression by targeting complementary or partially complementary mRNAs for degradation or translational inhibition. Recent studies have revealed that microRNAs play fundamental roles in plant growth and development, as well as in adaptation to biotic and abiotic stresses. This book highlights the roles of individual miRNAs that control and regulate diverse aspects of plant processes.

Mimicry is a classic example of adaptation through natural selection. The traditional focus of mimicry research has been on defence in animals, but there is now also a highly-developed and rapidly-growing body of research on floral mimicry in plants. This has coincided with a revolution in genomic tools, making it possible to explore which genetic and developmental processes underlie the sometimes astonishing changes that give rise to floral mimicry. Being literally rooted to one spot, plants have to cajole animals into acting as couriers for their pollen. Floral mimicry encompasses a set of evolutionary strategies whereby plants imitate the food sources, oviposition sites, or mating partners of animals in order to exploit them as pollinators. This first definitive book on floral mimicry discusses the functions of visual, olfactory, and tactile signals, integrating them into a broader theory of organismal mimicry that will help guide future research in the field. It addresses the fundamental question of whether the evolutionary and ecological principles that were developed for protective mimicry in animals can also be applied to floral mimicry in plants. The book also deals with the functions of floral rewardlessness, a condition which often serves as a precursor to the evolution of mimicry in plant lineages. The authors pay particular attention to the increasing body of research on chemical cues: their molecular basis, their role in cognitive misclassification of flowers by pollinators, and their implications for plant speciation. Comprehensive in scope and conceptual in focus, Floral Mimicry is primarily aimed at senior undergraduates, graduate students, and researchers in plant science and evolutionary biology.

It was around 1970, I had just completed a 5-year breeding project aiming at fxing fower colour in gerbera progenies: white, yellow, pink, and red; colour homogeneity was sound, but size and shape still required some improvement. The problem was defnitely resolved by Murashige and Skoog, USA who published a reliable protocol for gerbera micropro- gation. In short, my gerbera seed lines were immediately rendered obsolete by this e- cient cloning system, able to produce millions of plants of a matchless and previously unknown homogeneity, the uniformity of fower shape and colour being the basic requi- ments for the market. The success of micropropagation resulted in a tremendous growth in gerbera fower production worldwide, and this species conquered a leading place in the foriculture industry. This personal experience stresses the impact of micropropagation on the genetic improvement research strategies in ornamentals. Micropropagation has become "in- sive", especially in ornamental plant material issues. Today, hundreds of protocols exist; however, only a modest percentage of them are exploited economically. Thus, only micropropagation of plants with a high market price range, like orchids for instance, has proved cost-effective and achieved great success. Micropropagation is a labour-intensive system: hand-power is estimated to rep- sent 60-70% of total costs. This explains the outsourcing of the major labs in developing countries where labour is cheaper. Nevertheless, certain industrial protocols remain a proprietary technology of leading labs, mostly western, with the exception of Japan and Taiwan.

Optical screening of excessive and potentially harmful solar radiation is an important photoprotective mechanism, though it has received much less attention in comparison with other systems preventing photooxidative damage to photoautotrophic organisms. This photoprotection in the form of screening appears to be especially important for juvenile and senescing plants as well as under environmental stresses-i.e. in situations where the efficiency of enzymatic ROS elimination, DNA repair and other classical' photoprotective systems could be impaired. This book represents an attempt to develop an integral view of optical screening-based photoprotection in microalgae and higher plants. Towards this end, the key groups of pigments involved in the screening of ultraviolet and visible components of solar radiation in microalgae and higher plants, and the patterns of their accumulation and distribution within plant cells and tissues, are described. Special attention is paid to the manifestations of screening pigment accumulation in the optical spectra of plants. It is also demonstrated that understanding these effects and their relationships to screening pigments' makeup and spectroscopy in plants provides valuable insights into the state of plants' long-term photoacclimation, as well as ample opportunities for the non-destructive quantification of screening pigments and the assessment of the efficiency of photoprotection providing by these pigments in situ.

Oxygen (O ) appeared in significant amounts in the Earth's atmosphere over 2. 2 2 billion years ago, largely due to the evolution of photosynthesis by cyanobacteria (Halliwell 2006). The O molecule is a free radical, as it has two impaired electrons 2 that have the same spin quantum number. This spin restriction makes O prefer to 2 accept its electrons one at a time, leading to the generation of the so-called reactive oxygen species (ROS). The chemical nature of these species dictates that they can create damage in cells. This has contributed to the creation of the "oxidative stress" concept; in this view, ROS are unavoidable toxic products of O metabolism and 2 aerobic organisms have evolved antioxidant defences to protect against this tox- ity (Halliwell 1981; Fridovich 1998). Indeed, even in present-day plants, which are full of antioxidants, much of the protein synthetic activity of chloroplasts is used to replace oxidatively damaged D1 and other proteins (Halliwell 2006). Yet, the use of the "oxidative stress" term implies that ROS exert their effects through indiscriminate widespread inactivation of cellular functions. In this context, ROS must not be able to react with lipids, proteins or nucleic acids in order to avoid any damage to vital cellular components. However, genetic evidence has suggested that, in planta, purely physicoche- cal damage may be more limited than previously thought (Foyer and Noctor 2005).

Chloroplasts are vital for life as we know it. At the leaf cell level, it is common knowledge that a chloroplast interacts with its surroundings - but this knowledge is often limited to the benefits of oxygenic photosynthesis and that chloroplasts provide reduced carbon, nitrogen and sulphur. This book presents the intricate interplay between chloroplasts and their immediate and more distant environments. The topic is explored in chapters covering aspects of evolution, the chloroplast/cytoplasm barrier, transport, division, motility and bidirectional signalling. Taken together, the contributed chapters provide an exciting insight into the complexity of how chloroplast functions are related to cellular and plant-level functions. The recent rapid advances in the presented research areas, largely made possible by the development of molecular techniques and genetic screens of an increasing number of plant model systems, make this interaction a topical issue.

In any ecosystem, plant and microbe interaction is inevitable. They not only co-exist but also support each other's survival and also provide for sustenance in stressful environment. Agro-ecosystems of many regions around the globe are affected by multi-stress. Major limiting factors affecting the agricultural productivity worldwide are environmental stresses. Apart from decreasing yield they introduce devastating impact on plant growth as well. Plants battle with various kind of stresses with the help of symbiotic association with the microbes in the rhizosphere. Naturally existing plant-microbe interaction facilitates survival of plants under these stressful conditions. Rhizosphere consists of many groups of microbes, plant growth-promoting bacteria (PGPB) is one such group of microbes which assist plants in coping with multiple stresses and in plant growth as well. These microbes help in stress physiology of the plants and can be extremely useful in solving agricultural as well food security problems. The proposed book is split into two parts, with an aim to provide comprehensive description and highlight a holistic approach. It elucidates various mechanisms in rhizosphere of nutrient management, stress tolerance and enhanced crop productivity. The book discusses rhizospheric flora and its importance in enhancement of plant growth, nutrient content, yield of various crops and vegetables as well as soil fertility and health. Both volumes of the book addresses fundamentals, applications as well as research trends and new prospects of agricultural sustainability. Volume 2: Nutrient Management and Crop Improvement, contains chapters which cover a broad overview of plant growth promoting activities of microbes. This proposed book also highlights the contribution of nitrogen, phosphorus, potassium, iron and zinc-solubilizing microbes from rhizospheric soil to develop efficient indigenous microbial consortia to enhance the food and nutritional security. With the given content and layout the proposed book will be an all-inclusive collection of information, which will be useful for students, academicians, researchers working in the field of rhizospheric mechanisms, agricultural microbiology, soil microbiology, biotechnology, agronomy and sustainable agriculture and also for policy makers in the area of food security and sustainable agriculture. It will be of special interest to both academics and professionals working in the fields of microbiology, soil microbiology, biotechnology and agronomy, as well as the plant protection sciences. Timely, this edited and research book provides an essential and comprehensive source of material from basic to advance findings on microbes and their role in agricultural and soil sustainability.

Volume 2 covers nitrogen fertilizer efficiency, acid tolerance of the legume symbiosis, fruit tree nutrition, rhizosphere pH change, iron deficiency in crop production, the effects of nutrient deficiences on seed production, the elemental composition of plants, and the role of potassium. The articles in this volume join together both the fundamental and the applied parts of this discipline. The editors' aim to make the reviews comprehensible to scientists in relevant disciplines, rather than purely to the specialist. The format of each volume is a small number of full-length reviews of important topics, plus an editorial which briefly mentions other rapidly developing topics that may therefore be reviewed in future volumes.

In spite of international agreements at the political level not much has changed since the late 1980s in terms of reducing the speed of destruction of original tropical environments.

However, since the publication of the first edition ten years ago, international research efforts in physiological ecology of plants in the tropics has increased enormously in quantity and quality. In some fields advances were more substantial than in others. New approaches came up in remote sensing and at the other end of the scope in some areas molecular biology was particularly developed regarding ecological performance of tropical plants, e.g. in understanding the adaptation of resurrection plants to the extreme habitat of inselbergs.

The wealth of new information made it necessary to break large chapters down into smaller ones. Tropical forests which occupy about half of the entire volume of the book were now arranged in 5 chapters covering structure and function under the influence of environmental cues and including epiphytes and mangroves as part of the tropical forest complex. Savannas were now treated in two chapters. Coastal salinas have been combined with a new section on the Brazilian restingas in a chapter on coastal sand plains.

This book covers many facets of plant selenium (Se) accumulation: molecular genetics, biochemistry, physiology, and ecological and evolutionary aspects. Broader impacts and applications of plant Se accumulation also receive attention. Plant Se accumulation is very relevant for environmental and human health. Selenium is both essential at low levels and toxic at high levels, and both Se deficiency and toxicity are problems worldwide. Selenium can positively affect crop productivity and nutritional value. Plants may also be used to clean up excess environmental Se. Selenium in plants has profound ecological impact, and likely contributes to Se movement in ecosystems and global Se cycling.

Plant growth is of great economical and intellectual interest. Plants are the basis of our living environment, the production of our food and a myriad of plant-based natural products. Plant bio-mass is also becoming an important renewable energy resource.

Agricultural plant cultivation and breeding programs have altered plant productivity and yield parameters extensively, yet the principles and underlying mechanisms are not well understood. At the cellular level, growth is the result of only two processes, cell division and cell expansion, but these two processes are controlled by intertwined signaling cascades and regulatory mechanisms forming complex regulatory networks. Ultimately this network is what plant scientists are trying to unravel.

The sequencing of model and agronomically important plant genomes allows complete insight into the molecular components involved in each process. Methods to quantify the molecular changes, image growth processes and reconstruct growth regulatory networks are rapidly developing. This knowledge should help to elucidate key regulators and to design methods to engineer plant architecture and growth parameters for future human needs. This volume gives a comprehensive overview of what is known about plant growth regulation and growth restraints due to environmental conditions and should allow readers at all levels an entry into this exiting field of research.

"Use of Microbes for the Alleviation of Soil Stresses, Volume 1" describes the most important details and advances related to the alleviation of soil stresses by soil microbes. Comprised of seven chapters, the book reviews the mechanisms by which plant growth promoting rhizobacteria (PGPR) alleviate plant growth under stress; the role of mycorrhizal fungi on the alleviation of drought stress in host plants; how PGPR may alleviate salinity stress on the growth of host plants; and the role of PGPR on the growth of the host plant under the stress of sub optimal root zone temperature.

Written by experts in their respective fields, "Use of Microbes for the Alleviation of Soil Stresses, Volume 1 "is a comprehensive and valuable resource for researchers and students interested in the field of microbiology and soil stresses.

This volume describes different up-to-date methodological approaches, ranging from physiological assays to imaging and molecular techniques, to study a wide variety of plant responses to environmental cues. Environmental Responses in Plants: Methods and Protocols is divided into four sections: Tropisms, Photoperiodism and Circadian Rhythms, Abiotic Stress Responses, and Plant-Pathogen Interactions. The chapters in these sections include detailed protocols to investigate some of the many key biological processes underlying plant environmental responses, mostly in the model organism Arabidopsis thaliana, but also in Physcomitrella patens and in different crop species such as rice, potato, barley, or tomato. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Environmental Responses in Plants: Methods and Protocols, is a great resource for plant physiologists, biochemists, and cell and molecular scientists interested in this exciting and fast-growing research topic.

The interactions between the plant, soil and microbes are complex in nature. Events may be antagonistic, mutualistic or synergistic, depending upon the types of microorganisms and their association with the plant and soil in question. Multi-trophic tactics can therefore be employed to nourish plants in various habitats and growth conditions. Understanding the mechanisms of these interactions is thus highly desired in order to utilize the knowledge in an ecofriendly and sustainable way. This holistic approach to crop improvement may not only resolve the upcoming food security issues, but also make the environment greener by reducing the chemical inputs. Plant, soil and microbe, Volume 1: Implications in Crop Science, along with the forthcoming Volume 2: Mechanisms and Molecular Interactions, provide detailed accounts of the exquisite and delicate balance between the three critical components of agronomy. Specifically, these two titles focus on the basis of nutrient exchange between the microorganisms and the host plants, the mechanism of disease protection and the recent molecular details emerged from studying this multi-tropic interaction. Together they aim to provide a solid foundation for the students, teachers, and researchers interested in soil microbiology, plant pathology, ecology and agronomy.

Recherches Chimiques sur la Vegetation was a seminal work in the development of the understanding of photosythesis and plant chemistry. The original publication, which was the first concise summation of the basics of plant nutrition, was a landmark in plant science. It was twice translated into German during the nineteenth century, but no English translation has been published. This translation will interest those in the plant, chemical, agricultural, and soil sciences, and the history of science, who find English more accessible than French or German and who wish to learn more about the early research on photosynthesis and plant science. A further note about the translation: This project is more than just a translation because it includes an extensive introduction as well as notes that provide explanations for archaic terminology and other background material. In the twentieth century, eminent photosynthesis researcher Eugene Rabinowitch described Recherches Chimiques sur la Vegetation as the first modern book on plant nutrition. Historian of chemistry Henry Leicester called the book a classic, noting that the first important generalization about biochemistry in the nineteenth century came from it. Plant physiologist P. E. Pilet stated that the book laid the foundations of a new science, phytochemistry. Soil scientist E. Walter Russell attributed to de Saussure the quantitative experimental method, which more than anything else made modern agricultural chemistry possible. Chemist Leonard K. Nash stated that de Saussure brought the studies of plant nutrition begun by Priestley, Ingen-Housz, and Senebier close to completion, finishing the basic experimental work and providing a convincing theoretical interpretation of the field, and also opened up new vistas of experiment and thought. In the two centuries since Recherches Chimiques sur la Vegetation was published, luminaries in various branches of science, including plant biology, chemistry, and soil science, have consistently praised it highly. In the nineteenth century, noted botanist Alphonse de Candolle and equally noted plant physiologist Julius von Sachs expressed great admiration for it. Although de Saussure's ideas were forgotten for a time, famed chemist Justus von Liebig, who invented artificial fertilizer, rediscovered them in the 1840s and brought them to the attention of the agricultural community, stressing their importance for increasing crop yields.

Many plants produce enzymes collectively known as ribosome-inactivating proteins (RIPs). RIPs catalyze the removal of an adenine residue from a conserved loop in the large ribosomal RNA. The adenine residue removed by this depurination is crucial for the binding of elongation factors. Ribosomes modified in this way are no longer able to carry out protein synthesis. Most RIPs exist as single polypeptides (Type 1 RIPs) which are largely non-toxic to mammalian cells because they are unable to enter them and thus cannot reach their ribosomal substrate. In some instances, however, the RIP forms part of a heterodimer where its partner polypeptide is a lectin (Type 2 RIPs). These heterodimeric RIPs are able to bind to and enter mammalian cells. Their ability to reach and modify ribosomes in target cells means these proteins are some of the most potently cytotoxic poisons found in nature, and are widely assumed to play a protective role as part of the host plant's defenses. RIPs are able to further damage target cells by inducing apoptosis. In addition, certain plants produce lectins lacking an RIP component but which are also cytotoxic. This book focuses on the structure/function and some potential applications of these toxic plant proteins.

This book provides a knowledge-based view to the dynamic capabilities in an organization. The author integrates two existing views on gaining competitive advantage: the Knowledge View which suggests that the capability of organizations to learn faster than competitors is the only source of competitiveness; and the Dynamic Capability View which speculates that a fi rm's competitive advantage rests on it's ability to adapt to changes in the business environment. Using the IT sector in India as a case study, this book provides and tests a new framework-Knowledge-Based Dynamic Capabilities-in the prediction of competitive advantage in organizations.

In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, reagents, or software, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Metabolism: Methods and Protocols seeks to benefit scientists ranging from plant biology, metabolic engineering, and biotechnology.

This book details the plant-assisted remediation method, "phytoremediation", which involves the interaction of plant roots and associated rhizospheric microorganisms for the remediation of soil contaminated with high levels of metals, pesticides, solvents, radionuclides, explosives, crude oil, organic compounds and various other contaminants. Each chapter highlights and compares the beneficial and economical alternatives of phytoremediation to currently practiced soil removal and burial practices.

This book introduces the reader to synthetic or artificial seeds, which refer to alginate encapsulated somatic embryos, vegetative buds or any other micropropagules that can be used as seeds and converted into plantlets after propagating under in vitro or in vivo conditions. Moreover, synthetic seeds retain their potential for regeneration even after low-temperature storage. The production of synthetic or artificial seeds using micropropagules opens up new vistas in agricultural biotechnology. Encapsulated propagules could be used for in vitro regeneration and mass multiplication at reasonable cost. In addition, these propagules may be used for germplasm preservation of elite plant species and the exchange of plant materials between national and international laboratories. This book offers state-of-the-art findings on methods, applications and prospects of synthetic or artificial seeds.