This is the first of two volumes on Gentianaceae. Comprising twelve chapters, it centres upon the characterization and ecology of Gentianaceae worldwide, with emphasis on the application of molecular and cytological approaches in relation to taxonomy. The first three chapters consider the classification of the family and review the advances in research since the earlier revision published in 2002, which resulted in the reclassification of some plants and the naming of new genera. The next chapter provides the most comprehensive report to date of the systematics of South American Neotropical woody Gentians. Other reviews include details of the Gentianaceae in Eastern Europe. The key biochemical steps that result in the diversity of Gentian flower colors, the cytology of European species and an historical account of the importance of Gentians in herbal medicines are also covered. Furthermore, an analysis of gene expression in overwintering buds is presented, discussing several aspects of plant taxonomy, phenotypic characteristics, phylogeography and pedigree. Two contributions highlight the importance of Gentians in India, and the last chapter presents evidence for the importance of Glomeromycota in developing arbuscular mycorrhizal associations with the roots of Gentians.

This volume provides the basis for the biotechnological approaches that are considered in the companion book "The Gentianaceae Volume 2: Biotechnology and Applications.""

Plant-parasitic nematodes are among the most destructive plant pathogens, causing enormous losses to agronomic crops worldwide. This book provides an up-to-date review of research related to two of the most important nematode pests, root-knot and cyst nematodes. Chapters cover early plant-nematode interactions, identification of nematode proteins important in the establishment of nematode feeding sites, and classification of biochemical and signaling pathways significant in the development of specialized feeding sites in the host. The cellular and subcellular structures essential for the parasitic interaction are examined by light and electron microscopy. Modern techniques of gene expression analyses and genomic sequencing are poised to provide an even greater wealth of information to researchers, enabling them to develop and examine natural and manmade mechanisms of resistance to this important plant pest.

This book provides an excellent illustration of the interrelationship between progress in scientific methodology and conceptual advances, and its publica tion should contribute to further advances. It is well known that major advances in understanding often follow the development of new methods. The development of the acetylene reduction assay for nitrogenase activity provides a good example of this interrelationship between theory and methods. Theoretical knowledge led to a search for substrates for nitro genase that could be assayed for more easily than ammonium, the normal product of the enzyme. The discovery of the reduction of acetylene to ethylene by nitrogenase provided the ideal answer to the problem by provid ing a rapid, specific, nondestructive, and inexpensive assay for nitrogenase activity. This assay is now used by almost every laboratory doing research on nitrogen fixation. However, further use and development of the acetylene reduction assay has shown that it can underestimate nitrogenase activity and can even give incorrect relative values under some circumstances. The major problem is that exposure of legume nodules to acetylene can cause a large increase in the resistance to oxygen diffusion into the nodule. This reduced supply of oxygen decreases the rate of nitrogenase activity within a few minutes."

The study of plant cell expansion involves many different disciplines and technical approaches, and this book brings this diversity together to present a multifaceted view of the most up-to-date knowledge. Coverage includes data ranging from biophysical measurements and chemical analysis to molecular biological approaches and microscopy.

The plant cell wall plays a vital role in almost every aspect of plant physiology. New techniques in spectroscopy, biophysics and molecular biology have revealed the extraordinary complexity of its molecular architecture and just how important this structure is in the control of plant growth and development. The Second Edition of this accessible and integrated textbook has been revised and updated throughout. As well as focusing on the structure and function of plant cell walls the book also looks at the applications of this research. It discusses how plant cell walls can be exploited by the biotechnology industry and some of the main challenges for future research. Key topics include: architecture and skeletal functions of the wall; cell-wall formation; control of cell growth; role in intracellular transport; interactions with other organisms; cell-wall degradation; biotechnological applications of cell-walls; role in diet and health. This textbook provides a clear, well illustrated introduction to the physiology and biochemistry of plant cell walls which will be invaluable to upper level undergraduate and post graduate students of plant physiology, plant pathology, plant biotechnology and biochemistry.

This edited book highlights the plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species. Metabolic engineering is usually defined as the re-routing of one or more enzymatic reactions to generate new compounds, increase the production of existing compounds, or facilitate the degradation of compounds. Plants are the foundation of numerous compounds which are synthesized via assimilated complex biosynthetic routes. Plants have evolved an incredible arrangement of metabolic pathways leading to molecules/compounds capable of responding promptly and effectively to stress situations imposed by biotic and abiotic factors, some of which supply the ever-growing needs of humankind for natural chemicals, such as pharmaceuticals, nutraceuticals, agrochemicals, food and chemical additives, biofuels, and biomass. However, in foreseeable future we will be forced to think about the accessibility of resources for the generations to come. For these reasons, the book proposes alternative options of food/food supplement, medicines and other essential items, by using plant metabolic engineering approach. This book is of interest to teachers, researchers and academic experts. Also, the book serves as additional reading material for undergraduate and graduate students of biotechnology and molecular biology of plants.

Salinity is one of the acute problems causing enormous yield loss in many regions of the world. This phenomenon is particularly pronounced in arid and semiarid regions. Halophytes can remove salt from various types of problematic soils due to their unique morphological, physiological and anatomical adaptations to these environments. Halophytes are also used for the treatment of certain diseases but scientific documentation in terms of current phytotherapic applications is deficient in this unique group of plants. Different ethnic groups around the world use medicinal halophytes according to their own beliefs and ancestor’s experiences. However, their knowledge about the use of salt tolerant medicinal plants is usually confined to their own community. There is thus a knowledge gap on halophytes which should be bridged and preserved. This book provides a comprehensive account on the distribution of halophytes, their ethnobotanical and medicinal aspects, economic importance, and chemical constituents along with scientific description. The book therefore serves as a valuable resource for professionals and researchers working in the fields of plant stress biology and ethnobotanical aspects.

The rapid advances in elucidating the biosynthesis and mode of action of the plant hormone ethylene as well as its involvement in the regulation of the whole plant physiology made imperative the organization of a series of dedicated conferences. This volume contains the main lectures and poster contributions presented at the 7th International Symposium on the Plant Hormone Ethylene held in Pisa in 2006. The book is organized in seven sections dedicated to 1) Ethylene biosynthesis, perception and signal transduction, 2) Interactions between ethylene and other hormones, 3) Role of ethylene in plant growth and differentiation, 4) Fruit development, ripening and quality, 5) Abscission and senescence, 6) Ethylene involvement in biotic and abiotic stresses, and 7) Biotechnology and applied aspects.

In Flower Development: Methods and Protocols, researchers in the field detail protocols for experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls Authoritative and practical, Flower Development: Methods and Protocols is an essential guide for plant developmental biologists, from the novice to the experienced researcher, and for those considering venturing into the field.

Asexual reproduction is found in many taxonomic groups and considerable effort has been directed by biologists towards understanding its mechanisms, evolution and ecological significance. This research monograph, which is the culmination of several years of research by the author, offers a though-provoking contribution to this debate. It is primarily aimed at biologists undertaking research into the evolution, genetic control and ecological costs and benefits of different patterns of reproduction, although it should also be of interest to senior undergraduates.

Until very recently genetic maps of higher plants were based almost entirely on morphological and biochemical traits. These maps are rapidly being replaced and/or supplemented with DNA-based marker maps based on the use of powerful new molecular techniques. The new high precision maps can be developed with comparative ease and rapidity. They have a much higher density of markers, which allows revelation of more and more restricted segments of the genome. One of the many revolutionary aspects of this technology is that linkage between molecular markers and traits of interest often can be detected in a single cross. The ability to hybridize probe after probe to the DNA of the same individuals of a segregating population allows one to pursue the analysis until linkage becomes evident. With morphological and biochemical markers used previously, a separate cross was required to test linkage with each new marker. It was seldom that more than three markers could be tested for linkage with the trait of interest in a single cross because of viability problems. With the new techniques described in this volume, a new gene could be placed on the linkage map within a few days instead of the much longer time required with the previous techniques. In this book, a group of leading researchers provide background information and the latest versions of DNA-based marker maps for a variety of important crops. These maps illustrate the state of the art today. The progress made during the past five years has been truly phenomenal.

The sixth International Symposium on Genetics and Molecular Biology of Plant Nutriti9n was held in Elsinore, Denmark from August 17-21, 1998 and organised by th RiS0 National Laboratory in the year of its 40 anniversary. The 98 participants represented 23 countries and 80 scientific contributions with 43 oral and 37 poster presentations. The symposium addressed the molecular mechanisms, physiology and genetic regulation of plant nutrition. The Symposium brought together scientists from a range of different disciplines to exchange information and ideas on the molecular biology of mineral nutrition of plants. The symposium emphasised: * Bridging the gab between molecular biology, applied genetics, plant nutrition and plant breeding. * The development of methodologies to improve the efficiency and effectiveness of nutrition of plants * Quality of plant products. With sessions on: Nitrogen; Phosphorous; Micronutrients; Symbiosis; Membranes; Stress; Heavy Metals and Plant Breeding. In comparison with the previous conferences in this series more emphasis was placed on use of molecular techniques to clarify physiological mechanisms and processes, gene expression and regulation, as well as genetic marker assisted analysis. Significant of molecular genetic markers and other progress was reported in exploitation biotechnologies in breeding programmes.

Maize is one of the most generally grown cereal crops at global level, followed by wheat and rice. Maize is the major crop in China both in terms of yield and acreage. In 2012, worldwide maize production was about 840 million tons. Maize has long been a staple food of most of the global population (particularly in South America and Africa) and a key nutrient resource for animal feed and for food industrial materials. Maize belts vary from the latitude 58° north to the latitude 40° south, and maize ripens every month of the year. Abiotic and biotic stresses are common in maize belts worldwide. Abiotic stresses (chiefly drought, salinity, and extreme temperatures), together with biotic stresses (primarily fungi, viruses, and pests), negatively affect maize growth, development, production and productivity. In the recent past, intense droughts, waterlogging, and extreme temperatures have relentlessly affected maize growth and yield. In China, 60% of the maize planting area is prone to drought, and the resultant yield loss is 20%–30% per year; in India, 25%–30% of the maize yield is lost as a result of waterlogging each year. The biotic stresses on maize are chiefly pathogens (fungal, bacterial, and viral), and the consequential syndromes, like ear/stalk rot, rough dwarf disease, and northern leaf blight, are widespread and result in grave damage. Roughly 10% of the global maize yield is lost each year as a result of biotic stresses. For example, the European corn borer [ECB, Ostrinianubilalis (Hübner)] causes yield losses of up to 2000 million dollars annually in the USA alone in the northern regions of China, the maize yield loss reaches 50% during years when maize badly affected by northern leaf blight. In addition, abiotic and biotic stresses time and again are present at the same time and rigorously influence maize production. To fulfill requirements of each maize-growing situation and to tackle the above mentions stresses in an effective way sensibly designed multidisciplinary strategy for developing suitable varieties for each of these stresses has been attempted during the last decade.  Genomics is a field of supreme significance for elucidating the genetic architecture of complex quantitative traits and characterizing germplasm collections to achieve precise and specific manipulation of desirable alleles/genes. Advances in genotyping technologies and high throughput phenomics approaches have resulted in accelerated crop improvement like genomic selection, speed breeding, particularly in maize.  Molecular breeding tools like collaborating all omics, has led to the development of maize genotypes having higher yields, improved quality and resilience to biotic and abiotic stresses. Through this book, we bring into one volume the various important aspects of maize improvement and the recent technological advances in development of maize genotypes with high yield, high quality and resilience to biotic and abiotic stresses

Plant organelles have intrigued biologists since the discovery of their endosymbiontic origin and maternal inheritance. The first application of organelle biotechnology was the role of cytoplasmic male sterility in hybrid seed production and "Green Revolution." In modern times, plant organelles are again leading the way for the creation of genetically modified crops. On a global scale, 75% of GM crops are engineered for herbicide resistance and most of these herbicides target pathways that reside within plastids. Several thousand proteins are imported into chloroplasts that participate in biosynthesis of fatty acids, amino acids, pigments, nucleotides and numerous metabolic pathways including photosynthesis. Thus, from green revolution to golden rice, plant organelles have played a critical role in revolutionizing agriculture.

This book details not only basic concepts and current understanding of plant organelle genetics and molecular biology but also focuses on the synergy between basic biology and biotechnology. Forty four authors from nine countries have contributed twenty four chapters containing many figures and tables. Section 1 on organelle genomes and proteomes discusses molecular features of plastid and mitochondrial genomes, evolutinary origins, somatic and sexual inheritance, proteomics, bioinformatics and functional genomics. Section 2 on organelle gene expression and signalling discusses transcription, translation, RNA processing/editing, introns and splicing, protein synthesis, proteolysis, import of proteins into chloroplast and mitochondria and their regulation. Section 3 on organelle biotechnology discusses chloroplast and nuclear genetic engineering forbiotic/abiotic stress tolerance, improved fatty acid/amino acid biosynthesis, biopharmaceuticals, biopolymers and biomaterials, cytoplasmic male sterility for hybrid seed production, plant improvement and restoration of fertility.

This book is designed to serve as a comprehensive volume and reference guide for teachers, advanced undergraduates and graduate students and researchers in plant molecular biology and biotechnology.

Jatropha curcas, or physic nut, is a small tree that, in tropical climates, produces fruits with seeds containing ~38% oil. The physic nut has the potential to be highly productive and is amenable to subculture in vitro and to genetic modification. It also displays remarkable diversity and is relatively easy to cross hybridize within the genus. Thanks to these promising features, J. curcas is emerging as a promising oil crop and is gaining commercial interest among the biofuel research communities. However, as a crop, physic nut has been an economic flop since 2012, because the species was not fully domesticated and the average productivity was less than 2 t/ha, which is below the threshold of profitability.^7 t/ha could be reached and it is contributing to new markets in some countries. As such, it is important fro research to focus on the physiology and selective breeding of Jatropha . This book provides a positive global update on Jatropha, a crop that has suffered despite its promising agronomic and economic potential. The editors have used their collective expertise in agronomy, botany, selective breeding, biotechnology, genomics and bioinformatics to seek out high-quality contributions that address the bottleneck features in order to improve the economic trajectory of physic nut breeding.

This book focuses on the evolution of plant viruses, their molecular classification, epidemics and management, covering topics relating to evolutionary mechanisms, viral ecology and emergence, appropriate analysis methods, and the role of evolution in taxonomy. The currently emerging virus species are increasingly becoming a threat to our way of life, both economically and physically. Plant viruses are particularly significant as they affect our food supply and are capable of rapidly spreading to new plant species. In basic research, plant viruses have become useful models to analyze the molecular biology of plant gene regulation and cell-cell communication. The small size of DNA genome of viruses possesses minimal coding capacity and replicates in the host cell nucleus with the help of host plant cellular machinery. Thus, studying virus cellular processes provides a good basis for explaining DNA replication, transcription, mRNA processing, protein expression and gene silencing in plants. A better understanding of these cellular processes will help us design antiviral strategies for plants. The book provides in-depth information on plant virus gene interactions with hosts, localization and expression and the latest advances in our understanding of plant virus evolution, their responses and crop improvement. Combining characterization of plant viruses and disease management and presenting them together makes it easy to compare all aspects of resistance, tolerance and management strategies. As such, it is a useful resource for molecular biologists and plant virologists alike.

This book provides new insights into the mechanisms of plant hormone-mediated growth regulation and stress tolerance covering the most recent biochemical, physiological, genetic, and molecular studies. It also highlights the potential implications of plant hormones in ensuring food security in the face of climate change. Each chapter covers particular abiotic stress (heat stress, cold, drought, flooding, soil acidity, ozone, heavy metals, elevated CO2, acid rain, and photooxidative stress) and the versatile role of plant hormones in stress perception, signal transduction, and subsequent stress tolerance in the context of climate change. Some chapters also discuss hormonal crosstalk or interaction in plant stress adaptation and highlight convergence points of crosstalk between plant hormones and environmental signals such as light, which are considered recent breakthrough studies in plant hormone research. As exogenous application or genetic manipulation of hormones can alter crop yield under favorable and/or unfavorable environmental conditions, the utilization of plant hormones in modern agriculture is of great significance in the context of global climate change. Thus, it is important to further explore how hormone manipulation can secure a good harvest under challenging environmental conditions. This volume is dedicated to Sustainable Development Goals (SDGs) 2 and 13. The volume is suitable for plant science-related courses, such as plant stress physiology, plant growth regulators, and physiology and biochemistry of phytohormones for undergraduate, graduate, and postgraduate students at colleges and universities. The book can be a useful reference for academicians and scientists involved in research related to plant hormones and stress tolerance.

Environmental conditions and changes, irrespective of source, cause a variety of stresses, one of the most prevalent of which is salt stress. Excess amount of salt in the soil adversely affects plant growth and development, and impairs production. Nearly 20% of the world's cultivated area and nearly half of the world's irrigated lands are affected by salinity. Processes such as seed germination, seedling growth and vigour, vegetative growth, flowering and fruit set are adversely affected by high salt concentration, ultimately causing diminished economic yield and also quality of produce. Most plants cannot tolerate salt-stress. High salt concentrations decrease the osmotic potential of soil solution, creating a water stress in plants and severe ion toxicity. The interactions of salts with mineral nutrition may result in nutrient imbalances and deficiencies. The consequence of all these can ultimately lead to plant death as a result of growth arrest and molecular damage. To achieve salt-tolerance, the foremost task is either to prevent or alleviate the damage, or to re-establish homeostatic conditions in the new stressful environment. Barring a few exceptions, the conventional breeding techniques have been unsuccessful in transferring the salt-tolerance trait to the target species. A host of genes encoding different structural and regulatory proteins have been used over the past 5-6 years for the development of a range of abiotic stress-tolerant plants. It has been shown that using regulatory genes is a more effective approach for developing stress-tolerant plants. Thus, understanding the molecular basis will be helpful in developing selection strategies for improving salinity tolerance. This book will shed light on the effect of salt stress on plants development, proteomics, genomics, genetic engineering, and plant adaptations, among other topics. The book will cover around 25 chapters with contributors from all over the world.

This manual details the techniques involved in the study of plant microbe interactions (PMI). Covering a wide range of basic and advanced techniques associated with research on biological nitrogen fixation, microbe-mediated plant nutrient use efficiency, the biological control of plant diseases and pests such as nematodes, it will appeal to postgraduate students, research scholars and postdoctoral fellows, as well as teachers from various fields, including pathology, entomology and agronomy. It consists of five broad sections featuring different units. Information panels at the beginning of each unit present essential knowledge as well as advances in a particular topic. The manual can also serve as a textbook for undergraduate courses like Techniques for Plant-Microbe Interactions; Biological Control of Plant Diseases; and Nutrient Use Efficiency. Providing basic insights and working protocols from all related disciplines, this unique laboratory manual is a valuable resource for researchers interested in investigating PMI.

This book collects all the latest technologies with their implications on the global rice cultivation. It discusses all aspects of rice production and puts together the latest trends and best practices in the rice production. Rice is produced and consumed worldwide and especially an important crop for Asia. It is a staple food in majority of population living is this continent which distinguishes this from rest of the world. Climatic fluctuations, elevated concentrations of carbon dioxide, enhanced temperature have created extreme weather conditions for rice cultivation. Also, increasing pest attacks make situation complicated for the farmers. Therefore, rice production technology also has to be adjusted accordingly. This book is of interest to teachers, researchers, plant biotechnologists, pathologists, agronomists, soil scientists, food technologists from different part of the globe. Also, the book serves as additional reading material for students of agriculture, soil science, and environmental sciences. National and international agricultural scientists, policy makers will also find this to be a useful read

Photosynthesis and the Environment examines how photosynthesis may be influenced by environmental changes. Structural and functional aspects of the photosynthetic apparatus are examined in the context of responses to environmental stimuli; particular attention being given to the processing of light energy by thylakoids, metabolic regulation, gas exchange and source-sink relations. The roles of developmental and genetic responses in determining photosynthetic performance are also considered. The complexity of the responses to environmental change is demonstrated by detailed analyses of the effects of specific environmental variables (light, temperature, water, CO2, ozone and UV-B) on photosynthetic performance. Where appropriate attention is given to recent developments in the techniques used for studying photosynthetic activities.

The book is intended for advanced undergraduate and graduate students and a wide range of scientists with research interests in environmental effects on photosynthesis and plant productivity.

A growing interest has been shown recently in the dymanics of nitrogen in agricultural and natural ecosystems. This has been caused by increasing demands for food and fibre by a rapidly expanding world population, and by a growing concern that increased land clearing, cultivation and use of both fertilizer and biologically fixed nitrogen can have detrimental effects on the environment. These include effects on water quality, eutrophication of surface waters and changes in atmospheric composition all caused by increased cycling of nitrogenous compounds. The input and availability of nitrogen frequently affects the productivity of farming systems more than any other single management factor, but often the nitrogen is used inefficiently. Much of the fertilizer nitrogen applied to the soil is not utilised by the crop: it is lost either in solution form, by leaching of nitrate, or in gaseous forms as ammonia, nitrous oxide, nitric oxide or dinitrogen. The leached nitrate can contaminate rivers and ground waters, while the emitted ammonia can contaminate surface waters or combine with atmospheric sulfur dioxide to form aerosols which affect visibility, health and climate. There is also concern that increased evolution of nitrous oxide will deplete the protective ozone layer of the stratosphere. The possibility of a link between the intensity of agricultural use of nitrogen, nitrous oxide emissions and amounts of stratospheric ozone has focussed attention on these interactions.

This edited book covers all aspects of grain legumes including negative impact of abiotic and biotic stresses under the changing global climate. It discusses the role of various subject disciplines ranging from plant breeding, genetics, plant physiology, molecular biology, and genomics to high-throughput phenotyping and other emerging technologies for sustaining global grain and fodder legume production to alleviate impending global food crises. The book offers strategies to ensure plant-based dietary protein security across the globe. It covers all major commercial legume crops used as food, feed and fodder. This book is targeted to graduate and postgraduate students, researchers, progressive farmers and policymakers to inform them of the importance of cultivating grain and fodder legumes for future global food and nutritional security and for maintaining sustainable ecosystem.

The cell wall and its constituent polysaccharides and proteins control nearly all plant-based biological and biophysical processes. Understanding the cell wall is, therefore, not only fundamental to the plant sciences but is also pertinent to aspects of human and animal nutrition and health as well as plant-microbe and plant-animal interactions. In The Plant Cell Wall: Methods and Protocols, experts in the field describe detailed methods which are currently being applied to investigate the many aspects of the plant cell wall including its structure, biochemical composition, and metabolism. The book delves into a range of techniques involving plant tissue culture, which can be applied to investigating cell wall structure and metabolism, methods directed towards structural analysis and occurrence of carbohydrates, the development and use of microscopy-based tools and techniques, procedures which measure the physical properties of the wall, and methods based on the application of molecular genetic approaches. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, The Plant Cell Wall: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature.

This book discusses molecular approaches in plant as response to environmental factors, such as variations in temperature, water availability, salinity, and metal stress. The book also covers the impact of increasing global population, urbanization, and industrialization on these molecular behaviors. It covers the natural tolerance mechanism which plants adopt to cope with adverse environments, as well as the novel molecular strategies for engineering the plants in human interest. This book will be of interest to researchers working on the impact of the changing environment on plant ecology, issues of crop yield, and nutrient quantity and quality in agricultural crops. The book will be of interest to researchers as well as policy makers in the environmental and agricultural domains.