In any ecosystem, plant and microbe interaction is inevitable. They not only co-exist but also support each other's survival and provide sustenance in stressful environments. Agro-ecosystems in many regions around the globe are affected by high temperatures, soil salinity/alkalinity, low pH and metal toxicity. High salinity and severe draught are other major constraints affecting agricultural practices and also plants in the wild. A major limiting factor affecting global agricultural productivity is environmental stresses. Apart from decreasing yield, they also have a devastating impact on plant growth. Plants battle with various kind of stresses with the help of symbiotic associations with the rhizospheric microbes. Naturally occuring plant-microbe interactions facilitate the survival of plants under these stressful conditions. The rhizosphere consists of several groups of microbes, plant growth-promoting bacteria (PGPB) is one such group of microbes that assists plants in coping with multiple stresses and also promote plant growth. These efficient microbes support the stress physiology of the plants and can be extremely useful in solving agricultural as well food- security problems. This book provides a detailed, holistic description of plant and microbe interaction. It elucidates various mechanisms of nutrient management, stress tolerance and enhanced crop productivity in the rhizosphere, discussing The rhizospheric flora and its importance in enhancement of plant growth, nutrient content, yield of various crops and vegetables as well as soil fertility and health. Divided into two volumes, the book addresses fundamentals, applications as well as research trends and new prospects for agricultural sustainability. Volume 1: Stress Management and Agricultural Sustainability, includes chapters offering a broad overview of plant stress management with the help of microbes. It also highlights the contribution of enzymatic and molecular events occurring in the rhizosphere due to plant microbe interactions, which in turn help in the biological control of plant disease and pest attacks. Various examples of plant microbe interaction in rhizospheric soil are elaborated to facilitate the development of efficient indigenous microbial consortia to enhance food and nutritional security. Providing a comprehensive information source on microbes and their role in agricultural and soil sustainability, this timely research book is of particular interest to students, academics and researchers working in the fields of microbiology, soil microbiology, biotechnology, agronomy, and the plant protection sciences, as well as for policy makers in the area of food security and sustainable agriculture.

The openings offered by functional genomics reconciles organism biology and molecular biology, in order to define an integrative biology that should allow new insights about how a phenotype is built up from a genotype in interaction with its environment. This book covers a wide area of concepts and methods in genomics. This range from international genome sequencing projects to invaluable bioinformatics tools, analytical methods of gene expression, including final metabolic products, and their specific tissue to cellular compartmentalization. This new knowledge enables readers to understand the integration of basic physiological functions, and developmental programs. Also, the establishment of tight relationships between genomics, and genetics and plant breeding reveals synergies for exploiting molecular markers, for analysing genetic variation, or for studying quantitative traits. The integration that is now feasible by these new approaches also effects ecophysiology, and opens up new perspectives for the us

This book offers an up-to-date review of the regulatory role of nitric oxide (NO) changes in the morphological, physio-biochemical as well as molecular characteristics of plants under abiotic stress. The first of two parts comprises four chapters and focuses on the properties, chemical reactions involving NO and reactive nitrogen species in plants. The second part, consisting of eleven chapters, describes the current understanding of the role of NO in the regulation of gene expression, NO signaling pathways and its role in the up-regulation of the endogenous defense system and programmed cell death. Furthermore, its interactions with other signaling molecules and plant hemoglobins under environmental and soil related abiotic stresses, including post-harvest stress in fruits, vegetables and ornamentals and wounding are discussed in detail. Together with the companion book Nitric Oxide in Plants: Metabolism and Role in Stress Physiology, this volume provides a concise overview of the field and offers a valuable reference work for teachers and researchers in the fields of plant physiology, biochemistry and agronomy.

Molecular Physiology and Biotechnology of Trees, Volume 89 in the Advances in Botanical Research series, highlights new advances in the field, with this new volume presenting interesting chapters on such topics as the Activity of the shoot apical and cambial meristems: Coordination and responses to environmental signals, Conifer functional genomics, Nitrogen storage and cycling, Tree defense against pests and pathogens, The ectomycorrhizal contribution to tree nutrition, Phytoremediation with trees, Transcriptional regulation of wood formation, Transgenic poplars, the Genomics of forest trees, and much more.

The problems engendered by the conflicting imperatives of development and ecology show no sign of ending, and every day more locations are added to the list of landscapes poisoned by human activity. This vital book, featuring an international set of authors, is a key reference for researchers and environmental managers, as well as anyone involved in the mining industry or landscape remediation. The comprehensive coverage of current approaches to phytoremediation begins by examining the problem. It looks at natural and human-induced toxins, and their effects on natural vegetation as well as agricultural crops. Particular attention is paid to the two largest challenges to remediation -- heavy metals, and the salt stress that is impeding agricultural productivity worldwide. The text moves on to focus on the efficacy of different plant species in removing toxic pollutants from the environment. Along with analysis of a number of case studies, this section includes new and updated information on the mechanism of toxin-tolerance in plants.

A thorough understanding of the mechanisms of photosynthesis, regulation of structure and function and the adaptive strategies of oxygenic photosynthetic organisms is central to any effort directed at improving crop productivity and providing sustainable agriculture. Photosynthesis is the most widely researched topic in plant science. Further probing of its mechanisms, regulation and adaptation, employing a variety of modern tools and techniques, is imperative to gain a better insight of this very intricate process. Unravelling the cause of stress impairments and stress tolerance in plants would help in ensuring the optimum production of food, fibres and fuels. This book presents a study of photosynthesis and provides details of experimental approaches that have been adopted to understand the complex regulatory and adaptive processes. Its 27 chapters have been divided into four sections: "Evolution, Structure and Function"; "Biodiversity, Metabolism and Regulation"; "Stress and Adaptations"; and "Techniques". With contributions from leading subject experts from Australia, Canada, France, Germany, India, Israel, Japan, Sweden, Switzerland, the UK and USA, this comprehensive treatise

Contents:
1. Introduction to the Genus 2. Analysis and Quality Control of Commercial Artemisia Species 3. Artemisia absinthium 4. Artemisia dracunculus 5. Artemisia herba-alba 6. Ethnobothany, Phytochemistry and Biological/Pharmacological Activities of Artemisia ludoviciana ssp. Mexicana (Estafiate) 7. Artemisia pallens 8. Artemisia vulgaris 9. Artemisia Species in Traditional Chinese Medicine and the Discovery of Artemisinin 10. Cultivation of Artemisia annua L. 11. variation and Heritability of Artemisinin Content in Artemisia annua L. 12. Phytochemistry of Artemisia annua and the Development of Artemisinin-Derived Antimalarial Agents 13. The Mode of Action of Artemisinin and its Derivatives 14. The Clinical Use of Artemisinin and its Derivatives in the Treatment of Malaria 15. Regulation of the Quality and Use of Artemisinin and its Derivatives

The book examines the functions of the neurotransmitters acetylcholine and biogenic amines dopamine, noradrenaline, serotonin, and histamine in plant organisms. Also addressed are how many plant reactions are sensitive to neurotransmitters and their significance in the field of medicine. Papers in the collecting describe participation of the components of animal cholinergic and aminergic regulatory systems in the functioning of many plant processes within and outside the cell, from changes in ion permeability of membranes, energetics, and metabolism to complex processes such as fertilization, motility, and finally germination, growth, and morphogenesis.

Now in its third edition, this book describes photosynthesis by considering the partial processes involved throughout the various level of plant (cell, organelle and molecule), and their contributions to the complete process. It also considers the molecular and biochemical events which take place in the photosynthetic system. Finally it explains how these determine the physiological characteristics of plant productivity. The new edition has been extensively revised with comprehensively updated references and many new figures. It includes a new chapter on the applications of genetic modification of photosynthesis systems.

Covers the basic knowledge of the regulation of biosynthesis of various amino acids in plants and the application of this knowledge to the discovery of novel inhibitors of amino acid biosynthesis and for enhancing the nutritional value of plant products. Provides an exhaustive list of pathway inhibitors.

Primary Active Transporters: A Plethora of Plant Plasmalemma Proton Pumps (M.R. Sussman). Studies on the Higher Plant CalmodulinStimulated ATPase (D.E. Evans et al.). Secondary Ion and Metabolite Transporters: ProtonSugar Cotransporters in Plants (N. Sauer). Insights into the Structure of the Chloroplast Phosphate Translocator Protein (H. Wallmeier et al.). Channel Proteins: Soybean Nodulin26 (D.P.S. Verma). Putative LType Calcium Channels in Plants (R. Ranjeva et al.). Receptor Proteins: Hormone Perception and Signal Transduction in Aleurone (R. Hooley et al.). The Auxin Receptor (R.M. Napier, M.A. Venis). Protein Targeting and Assembly in Membranes: Sequence Determinants for Protein Import into Chloroplasts and Thylakoid Membrane Protein Assembly (G. von Heijne). 11 additional articles. Index.

Plants are sessile organisms that live under a constant barrage of biotic and abiotic insults. Both biotic and abiotic stress factors have been shown to affect various aspects of plant system including the acceleration in the formation of reactive oxygen species (ROS). The ascorbate (AsA)-glutathione (GSH) pathway is a key part of the network of reactions involving enzymes and metabolites with redox properties for the detoxification of ROS, and thus to avert the ROS-accrued oxidative damage in plants. The present book mainly deals with the information gained through the cross-talks and inter-relationship studies on the physiological, biochemical and molecular aspects of the cumulative response of various components of AsA-GSH pathway to stress factors and their significance in plant stress tolerance.

Abiotic stresses such as high temperature, low-temperature, drought, and salinity limit crop productivity worldwide. Understanding plant responses to these stresses is essential for rational engineering of crop plants. In Arabidopsis, the signal transduction pathways for abiotic stresses, light, several phytohormones and pathogenesis have been elucidated. A significant portion of plant genomes (most studies are Arabidopsis and rice genome) encodes for proteins involves in signaling such as receptor, sensors, kinases, phosphatases, transcription factors and transporters/channels. Despite decades of physiological and molecular effort, knowledge pertaining to how plants sense and transduce low and high temperature, low-water availability (drought), water-submergence and salinity signals is still a major question before plant biologists. One major constraint hampering our understanding of these signal transduction processes in plants has been the lack or slow pace of application of molecular genomic and genetics knowledge in the form of gene function. In the post-genomic era, one of the major challenges is investigation and understanding of multiple genes and gene families regulating a particular physiological and developmental aspect of plant life cycle. One of the important physiological processes is regulation of stress response, which leads to adaptation or adjustment in response to adverse stimuli. With the holistic understanding of the signaling pathways involving not only one gene family but multiple genes or gene families, plant biologists can lay a foundation for designing and generating future crops that can withstand the higher degree of environmental stresses (especially abiotic stresses, which are the major cause of crop loss throughout the world) without losing crop yield and productivity.

This volume brings together all aspects of TAXOLA(R) research, development, and clinical use. It provides comprehensive knowledge of the compound and a perspective of the complex interrelationships needed for its development and production. Each chapter is written by an authority in the field. Chapters are carefully coordinated to maximize information on key topics while avoiding overlap and duplication. Previously unpublished material is presented along with thorough reviews of each topic.

The "Phytochemical Dictionary of the Leguminosae" is the first of a new type of reference source giving phytochemical records for all legumes (plants in the Pea family - Leguminosae or Fabaceae). The precise chemical substances found, the organs in which they occur (eg the leaf or the seed) and the bibliographic citation are given for each plant species recorded. These are accompanied by extensive supporting botanical, chemical, geographical and bibliographic information for each plant and substance. Over 4,000 chemical substances occurring in 2,000 plant species are contained within 20,000 entries. The Leguminosae is one of the world's most economically important groups of plants, including peas, beans, soya and chickpeas, and provides the world's major source of nitrogen fixed from the atmosphere. As this book contains detailed, comprehensive and up-to-date phytochemical data on this family, available for the first time in a single source, it will prove invaluable to all those working in the food, pharmaceutical and agrochemical industries, as well as in botanical, natural product and taxonomic research. This new work has been compiled as a joint project by two specialist organisations, the International Legume Database & Information Service (ILDIS) and the Chapman & Hall Chemical Database (CHCD). Coverage includes both wild and cultivated species from all over the world. The primary literature used is current to mid-1992. The book is divided into two volumes, the first containing the Plant Section and the second the Chemical Section. The two are linked by extensive cross-referencing and each section has its own indexes. Volume 1 of the dictionary is unique in that it lists not only all legume species from which chemical substances are reported, but also lists under each species what the substances are and in which organs they occur. The substances are grouped together under types of compounds and the original sources are cited. This part is organized alphabetically by genus and species name, followed by a Plant Name Index and Plant Constituent Index. This part of the dictionary may be used in two ways. By selecting a plant species of interest, the reader will see the precise listing of substances reported and the organs in which they occur. Alternatively, by using the Plant Constituent Index, the reader should find a full listing of all legume species from which a particular substance is reported, and from which the main entries for these plant species can be located. Each species entry is annotated with the plant's geographical distribution, its taxonomic details (common name, synonyms used in the phytochemical literature, etc.), botanical data on, for example, life form and economic uses, and reference citations. The problems of nomenclature and synonymy have been overcome for both plant names and substance names. Plant names and classification have been verified using the ILDIS plant taxonomic database: records for the same species originally published under different names are united in the dictionary. Similarly, substance names and classes have been verified using the Chapman & Hall Chemical Database: records for the same substance under different names in the literature are likewise united in the dictionary. Volume 2 is a Chemical Dictionary giving key chemical data on all substances occurring in the Leguminosae, matching those reported in Part 1. This part is taken from the Chapman & Hall Chemical Database and its layout and format is uniform with the renowned Dictionary of Organic Compounds. Each substance has (where appropriate): alternative names, structure diagram, Chemical Abstracts Service (CAS) Registry Number, molecular formula and weight and Type of Compound. These substances are indexed by Chemical Name, Molecular Formula and CAS Registry Number to allow rapid location of the information required.

This book presents a holistic view of the complex and dynamic responses of plants to nanoparticles, the signal transduction mechanisms involved, and the regulation of gene expression. Further, it addresses the phytosynthesis of nanoparticles, the role of nanoparticles in the antioxidant systems of plants and agriculture, the beneficial and harmful effects of nanoparticles on plants, and the application of nanoparticles and nanotubes to mass spectrometry, aiming ultimately at an analysis of the metabolomics of plants. The growing numbers of inventions in the field of nanotechnology are producing novel applications in the fields of biotechnology and agriculture. Nanoparticles have received much attention because of the unique physico-chemical properties of these compounds. In the life sciences, nanoparticles are used as "smart" delivery systems, prompting the Nobel Prize winner P. Ehrlich to refer to these compounds as "magic bullets." Nanoparticles also play an important role in agriculture as compound fertilizers and nano-pesticides, acting as chemical delivery agents that target molecules to specific cellular organelles in plants. The influence of nanoparticles on plant growth and development, however, remains to be investigated. Lastly, this book reveals the research gaps that must be bridged in the years to come in order to achieve larger goals concerning the applications of nanotechnology in the plants sciences. In the 21st century, nanotechnology has become a rapidly emerging branch of science. In the world of physical sciences, nanotechnological tools have been exploited for a broad range of applications. In recent years, nanoparticles have also proven useful in several branches of the life sciences. In particular, nanotechnology has been employed in drug delivery and related applications in medicine.

The adaptation of desirable agricultural plants to infertile and problem soils is an increasingly important strategy for improving food supplies in many parts of the world. The plant breeding approach complements, and in some cases may replace agronomic practices such as the use of fertilizers and soil amendments to provide solutions which are economically and environmentally sustainable. The Symposium at which the papers in this volume were presented drew together workers in plant breeding, plant nutrition, physiology, biochemistry and molecular biology to discuss research on gene systems which affect the mineral nutrition of plants. Papers describe successes in plant breeding for problem soils as well as advances in understanding of mechanisms at the whole plant and cellular levels. Papers in the "molecular" area point the way to the contribution which the new biology will make to this field in the future. The reviews and research papers are grouped under five topics: better plants for acid soils; salinity tolerance; efficiency of uptake and use of macronutrients; efficiency for iron and micronutrients; and tolerance of heavy metals and boron.

Several fundamental advances were announced at the Seventh International Symposium on Molecular Plant--Microbe Interactions held in Edinburgh in 1994. These included the cloning and identification of plant resistance genes involved in recognition of pathogens; the description of genetically engineered plants with novel resistance to pathogens; characterization of the molecular basis of pathogenicity of fungal and bacterial plant pathogens; and the mechanisms of communication used during recognition between symbiotic rhizobia and their host legumes. Participants in the Symposium contributed a series of papers that represent the leading edge of research in this important area of plant and microbial science. These articles are brought together to form this book, which will be essential reading for research workers, advanced students and others interested in keeping abreast of this rapidly developing area.

The Fifth International Symposium on Nitrogen Fixation with Non-legumes was held in Florence (Italy) on 10-14 September, 1990. Earlier Symposia of this series were held in Piracicaba (Brazil), Banf Alberta (Canada), Helsinki (Finland) and Rio De Janeiro (Brazil). The Symposium's main objectives were to bring together scientists working in many different fields of nitrogen fixation, to stimulate discussion on this important process and to have an appraisal of the most recent studies concerning nitrogen fixation with non-legumes. The Symposium was attended by 230 scientists from 32 different countries. This volume collects the contributions of 65 lectures and 87 posters, which are an up-to-date account of the state of knowledge on biological nitrogen fixation with non-legumes. The book provides a valuable reference source not only for specialists in nitrogen fixation, but also for researchers working on related aspects of agronomy, biochemistry, genetics, microbiology, molecular biology and plant physiology. It is with great pleasure that we aknowledge the contributions of the authors in assuring the prompt pubblication of this volume. We would also like to express our thanks to Kluwer Academic Publishers B.V. for the publication of these Proceedings. M. Polsinelli R. Materassi M. Vincenzini ORGANIZING COMMITTEE President M. Polsinelli M. Vincenzini Secretary F. Favilli Treasurer E. Galli E. Gallori L. Giovannetti R. Materassi M.P. Nuti M.R. Tredici SCIENTIFIC COMMITTEE M. Bazzicalupo Florence, Italy H. Bothe Cologne, West Germany R.H. Burris Madison, U.S.A.

Research on the mechanisms of plant defense responses to stress and pathogen attack has attracted much attention in recent years. This increasing interest stems from the fact that the tools of molecular biology now enable us to study the molecular basis of old biological concepts such as host-pathogen recognition (and particularly the gene for-gene relationship), hypersensitive cell death and systemic acquired resistance. Our knowledge about avirulence and resistance genes, elicitors, signal transduction and genes involved in plant defense is rapidly expanding. Moreover we are just beginning to test in planta the potential of these results for biotechnological applications, aimed at improving plant resistance to diseases. The 2nd Conference of the European Foundation for Plant Pathology, hosted by the "Societe Fran~aise de Phytopathologie", was devoted to "Mechanisms of plant defense responses" and was held in Strasbourg, France. It brought together over 350 scientists from universities, research institutes and private sectors of 24 countries. Major advances in the areas under study have been reviewed in plenary lectures and are developed in the main articles of this book. Over 160 high-quality posters were presented and are summarized in short articles. Data from outstanding posters, which were discussed after a short oral presentation, are found in extended articles. As a whole the book presents a collection of papers arranged in six sections and reflecting the present day state-of-the-art of research in the field of plant defense reactions.

Considerable advances have been made in our understanding of the eukaryotic cell cycle at the molecular level over the past two decades or so, particularly in yeast and in animal systems. However, only in the past three or four years has progress been made in plants at the molecular level. The present volume brings together molecular biologists, cell biologists and physiologists to discuss this recent progress and how it relates to our understanding of the regulation of plant growth and development. The opening paper summarises the progress which has been made with fission yeast. Subsequent papers explore what is known about cell cycle control at the molecular level in plants, and about cell cycle regulation in specific physiological systems, ending with summary papers on cell division in roots and shoots. The book comprises up-to-date findings on a fundamental aspect of plant growth and development, and as such will be of particular interest to advanced undergraduates, postgraduates and research scientists in the fields of molecular biology, cell biology and physiology.

Significant developments in recent years have led to a deeper understanding of the role and function of carotenoids in photosynthesis. For the first time the biological, biochemical, and chemical aspects of the role of these pigments in photosynthesis are brought together in one comprehensive reference volume. Chapters focus on the photochemistry of carotenoids in light harvesting and photoprotection, the nature and distribution of carotenoids in photosynthetic organisms, their biosynthesis, the herbicidal inhibition of carotenogenesis and the xanthophyll cycle'. Throughout details are given of the various methodologies used. A detailed appendix provides physical data for the major compounds. Carotenoids in Photosynthesis is an invaluable reference source for all plant scientists.

JACQUES S. BECKMANN & THOMAS C. OSBORN Extraordinary progress has been made in the analyses of the genetic structures of higher eukaryotic genomes. Only ten years elapsed between the initial proposals to use molecular DNA markers for the generation of a complete linkage map of the human genome [5, 17] and the first description of a 10 centimorgan map of one of its chromosomes [22], soon to be followed by others. The availability of molecular DNA markers, henceforth called genomic markers [for a review of their properties see 1, 2, 20], represents a milestone in genetics by providing the capacity for complete genetic coverage of all genomes. It is important to remember that the nature of the DNA polymorphism or of the specific method used to uncover it can be quite different for different marker loci. The genetic variation detected can be a result of a simple point mutation, a DNA insertion/deletion event, or a change in repeat copy number at some hypervariable DNA [11] or micro satellite [21] motif. Currently, the methods of detection can involve use of restriction endonucleases, nucleic acid hybridization, or DNA sequence amplification. Each of these sources of var iation and methods of detection can have utility for different applications. Furthermore, new approaches for the detection of DNA polymorphism are constantly emerging. The primary concern here is that the monitored poly morphism defines a genetic marker 'useful' for the desired application.

Over the past decade, advances in molecular biology have provided the impetus for a resurgence of interest in plant metabolism. At a general level, the potential for modifying the quantity or quality of harvestable crop products through genetic manipulation has provided an agronomic rationale for seeking a greater understanding of primary plant metabolism and its regulation. Moreover, the now facile techniques for transformation of many plant species and the consequential capacity to manipulate the amounts of specific individual enzymes within specific cell types provides an exciting direct approach for studying metabolic problems. Such transgenic plants are also becoming invaluable tools in studies at the interface between metabolism and other sub-disciplines such as physiology and ecology. The interest generated in plant metabolism by these developments has also encouraged the re-introduction of more conventional biochemical techniques for metabolic analysis. Finally, in common with other areas of cell biology, the wealth of information that can be obtained at the nucleic acid level has provided the stimulus for identification and characterisation of metabolic processes in far greater detail than previously envisaged. The result of these advances it that researchers now have the confidence to address problems in plant metabolism at levels not previously attempted. This book presents the proceedings of an international conference held on 9-11 January 1997 at St Hugh's College, Oxford under the auspices of the Phytochemical Society of Europe.

Higher eukaryotes are characterized by the allocation of distinct functions to numerous types of differentiated cells. Whereas in animals the well-defined, protected cells of the germ line separate early, germ cells in plants differentiate from somatic cells only after many cycles of mitotic division. Therefore somatic mutations in plants can be transmitted via the germ cells to the progeny. There is thus a clear need for somatic tissues to maintain their genetic integrity in the face of environmental challenges, and two types of interactions have been shown to play important roles in the conservation as well as flexibility of plant genomes: homologous recombination of repeated sequences and silencing of multiplied genes. Sensitive methods have been developed that allow greater insights into the dynamics of the genome. This book summarizes current knowledge and working hypotheses about the frequencies and mechanisms of mitochondrial, plastid, nuclear and viral recombination and the inactivation of repeated genes in plants. Despite rapid developments in the field, it is often not possible to provide final answers. Thus, it is an additional task of this book to define the open questions and future challenges. The book is addressed to scientists working on plant biology and recombination, to newcomers in the field and to advanced biology students.