This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Pattern Recognition Receptors: Methods and Protocols aims to ensure successful results in the further study of this vital field.

The functional characterization of a key enzyme in the phosphatidylinositol (PI) signaling pathway in the model plant Arabidopsis thaliana is the focus of the research summarised in this thesis. Moreover, a particular focus is the exploration of the biological functions of Arabidopsis phophatidylinositol monophosphate 5-kinase 2 (PIP5K2) which catalyzes the synthesis of phophatidylinositol (4,5) bisphosphate, the precursor of two important second messengers (inositol 1,4,5-trisphosphate and diacylglycerol). Through molecular and genetic approaches, the author isolated and characterized the expression pattern, physiological functions and the underlying mechanism of Arabidopsis PIP5K2. It is found that PIP5K2 is involved in regulating lateral root formation and root gravity response through modulating auxin accumulation and polar auxin transport and also plays a critical role in salt tolerance. These findings shed new light on the crosstalk between PI signaling and auxin response, both of which have crucial regulatory roles in plant development.

This volume illustrates the complex root system, including the various essential roles of roots as well as their interaction with diverse microorganisms localized in or near the root system.

Following initial chapters describing the anatomy and architecture as well as the growth and development of root systems, subsequent chapters focus on the various types of root symbiosis with bacteria and fungi in the rhizosphere. A third section covers the physiological strategies of roots, such as nitrate assimilation, aquaporins, the role of roots in plant defense responses and in response to droughts and salinity changes. The book s final chapters discuss the prospects of applied engineering of roots, i.e., inventing new root structures or functions through genetic modification, but also with conventional breeding and manipulation of root symbionts. The budding field of root engineering is expected to promote a second green revolution."

This collection discusses the variety of specific molecular reactions by means of which plants respond to physiological and toxic stress conditions. It focuses on the characterization of the molecular mechanisms that underlie the induction of toxicity and the triggered responses and resistances. The nine chapters, all written by prominent researchers, examine heavy metal toxicity, aluminum toxicity, arsenic toxicity, salt toxicity, drought stress, light stress, temperature stress, flood stress and UV-B stress. In addition, information on the fundamentals of stress responses and resistance mechanisms is provided. The book addresses researchers and students working in the fields of plant physiology and biochemistry.

This edited volume focuses on the characterization, reclamation, bioremediation, and phytoremediation of salt affected soils and waterlogged sodic soils. Innovative technologies in managing marginal salt affected lands merit immediate attention in the light of climate change and its impact on crop productivity and environment. The decision-making process related to reclamation and management of vast areas of salt affected soils encompasses consideration of economic viability, environmental sustainability, and social acceptability of different approaches. The chapters in this book highlight the significant environmental and social impacts of different ameliorative techniques used to manage salt affected soils. Readers will discover new knowledge on the distribution, reactions, changes in bio-chemical properties and microbial ecology of salt affected soils through case studies exploring Indian soils. The contributions presented by experts shed new light on techniques such as the restoration of degraded lands by growing halophyte plant species, diversification of crops and introduction of microbes for remediation of salt infested soils, and the use of fluorescent pseudomonads for enhancing crop yields.

r ed Algae in Genome Age book most people reading this book have childhood memories about being enthralled at the beach with those rare and mysterious living forms we knew as seaweeds. We were fascinated at that time by their range of red hues and textures, and most of all, their exotic beauty. t o a scientist, red algae represent much more than apparent features. t heir complex forms have attracted morphologists for centuries; their intricate life cycles have brought more than one surprise to plant biologists familiar only with ferns and fowering plants; their unusual tastes have been appreciated for mill- nia, and their valuable chemical constituents have been exploited for nearly as long, most recently by biotech companies; their diversity in marine, freshwater, and t- restrial environments has offered centuries of engaging entertainment for botanists eager to arrange them in orderly classifcation systems; still, the red algae continue to teach us how many more challenges need to be overcome in order to understand their biodiversity, biological functions, and evolutionary histories.

The book focuses on the principles and practices of tropical maize improvement with special emphasis on early and extra-early maize to feed the increasing population in Sub-Saharan Africa. It highlights the similarities and differences between results obtained in temperate regions of the world and WCA in terms of corroboration or refutation of genetic principles and theory of maize breeding. The book is expected to be of great interest to maize breeders, advanced undergraduates, graduate students, professors and research scientists in the national and international research institutes all over the world, particularly Sub-Saharan Africa. It will also serve as a useful reference for agricultural extension and technology transfer systems, Non-governmental Organizations (NGOs) and Community-Based Organizations (CBOs), seed companies and community-based seed enterprises, policy makers, and all those who are interested in generating wealth from agriculture and alleviating hunger and poverty in Sub-Saharan Africa.

The present edited book is an attempt to update the state of art of the knowledge on metabolism of ROS and antioxidants and their relationship in plant adaptation to abiotic stresses involving physiological, biochemical and molecular processes. The chapters are much focused on the current climate issues and how ROS metabolism can manipulate with antioxidant system to accelerate detoxification mechanism. It will enhance the mechanistic understanding on ROS and antioxidants system and will pave the path for agricultural scientists in developing tolerant crops to achieve sustainability under the changing environmental conditions. The increase in abiotic stress factors has become a major threat to sustainability of crop production. This situation has led to think ways which can help to come out with potential measures; for which it is necessary to understand the influence of abiotic stress factors on crops performance and the mechanisms by which these factors impact plants. It has now become evident that abiotic stress impacts negatively on plant growth and development at every stage of plant's life. Plants adapt to the changing environment with the adjustment at physiological, biochemical and molecular levels. The possible mechanisms involved in the negative effects of abiotic stress factors are excess production of reactive oxygen species (ROS). They alter physiological and molecular mechanisms leading to poor performance of plants. Plants however, are able to cope with these adverse effects by inducing antioxidant systems as the priority. Nevertheless, the dual role of ROS has now been ascertained which provides an evidence for regulation of plant metabolism positively on a concentration-dependent manner. Under conditions of high ROS production, the antioxidant system plays a major role in diminishing the effects of ROS. Thus, ROS production and antioxidant system are interwoven with abiotic stress conditions. The antioxidants have the capacity to hold the stability in metabolism in order to avoid disruption due to environmental disturbances.

Cancer is one of the leading death cause of human population increasingly seen in recent times. Plants have been used for medicinal purposes since immemorial times. Though, several synthetic medicines are useful in treating cancer, they are inefficient and unsafe. However, plants have proved to be useful in cancer cure. Moreover, natural compounds from plants and their derivatives are safe and effective in treatment and management of several cancer types. The anticancer plants such as Catharanthus roseus, Podophyllum peltatum, Taxus brevifolia, Camptotheca acuminate, Andrographis paniculata, Crateva nurvala, Croton tonkinensis, Oplopanax horridus etc., are important source of chemotherapeutic compounds. These plants have proven their significance in the treatment of cancer and various other infectious diseases. Nowadays, several well-known anticancer compounds such as taxol, podophyllotoxins, camptothecin, vinblastine, vincristine, homoharringtonine etc. have been isolated and purified from these medicinal plants. Many of them are used effectively to combat cancer and other related diseases. The herbal medicine and their products are the most suitable and safe to be used as an alternative medicine. Based on their traditional uses and experimental evidences, the anticancer products or compounds are isolated or extracted from the medicinally important plants. Many of these anticancer plants have become endangered due to ruthless harvesting in nature. Hence, there is a need to conserve these species and to propagate them in large scale using plant tissue culture. Alternatively, plant cell tissue and organ culture biotechnology can be adopted to produce these anticancer compounds without cultivation. The proper knowledge and exploration of these isolated molecules or products could provide an alternative source to reduce cancer risk, anti-tumorigenic properties, and suppression of carcinogen activities. Anticancer plants: Volume 1, Properties and Application is a very timely effort in this direction. Discussing the various types of anticancer plants as a source of curative agent, their pharmacological and neutraceutical properties, cryo-preservations and recent trends to understand the basic cause and consequences involved in the diseases diagnosis. We acknowledge the publisher, Springer for their continuous inspiration and valuable suggestions to improvise the content of this book. We further extend our heartfelt gratitude to all our book contributors for their support, and assistance to complete this assignment. I am sure that these books will benefit the scientific communities including academics, pharmaceuticals, nutraceuticals and medical practitioners.

Plants live in a constantly changing environment from which they cannot physically escape. Plants therefore need signalling and response mechanisms to adapt to new local conditions. The ef?cacy of such mechanisms underlies the plant performance during stress and therefore also impacts greatly on agricultural productivity. M- ulation of ion channel activity not only provides a means for rapid signal generation 2+ but also allows adjustment of cellular physiology. For example, Ca permeable ion 2+ channels can transduce environmental stimuli into Ca -encoded messages which can modify the gene expression. Furthermore, ion channel activity is essential to control cellular ion homeostasis that impacts on plant responses to drought, salinity, pathogens, nutrient de?ciency, heavy metals, xenobiotics and other stresses. This volume focuses on the crucial roles of different types of ion channel in plant stress responses. Functions of ion channels are discussed in the context of mechanisms to relay external and endogenous signals during stress and as mechanisms to regulate cellular ion homeostasis and enzymatic activities in the context of biotic and abiotic stress. The chapters presented cover cation and anion channels located in various cellular compartments and tissues.

Precise regulation of gene expression in both time and space is vital to plant growth, development and adaptation to biotic and abiotic stress conditions. This is achieved by multiple mechanisms, with perhaps the most important control being exerted at the level of transcription. However, with the recent discovery of microRNAs another ubiquitous mode of gene regulation that occurs at the post-transcriptional level has been identified. MicroRNAs can silence gene expression by targeting complementary or partially complementary mRNAs for degradation or translational inhibition. Recent studies have revealed that microRNAs play fundamental roles in plant growth and development, as well as in adaptation to biotic and abiotic stresses. This book highlights the roles of individual miRNAs that control and regulate diverse aspects of plant processes.

Considerable advances have been made in our understanding of the eukaryotic cell cycle at the molecular level over the past two decades or so, particularly in yeast and in animal systems. However, only in the past three or four years has progress been made in plants at the molecular level. The present volume brings together molecular biologists, cell biologists and physiologists to discuss this recent progress and how it related to our understanding of the regulation of plant growth and development. The opening paper summarises the progress which has been made with fission yeast. Subsequent papers explore what is known about cell cycle control at the molecular level in plants, and about cell cycle regulation in specific physiological systems, ending with summary papers on cell division in roots and shoots. The book comprises up-to-date findings on a fundamental aspect of plant growth and development, and as such should be of particular interest to advanced undergraduates, postgraduates and research scientists in the fields of molecular biology, cell biology and physiology.

The entire range of the developmental processes in plants is regulated by a shift in the hormonal concentration, tissue sensitivity and their interaction with the factors operating around them. Out of the recognized hormones, attention has largely been focused on five - Auxins, Gibberellins, Cytokinin, Abscisic acid and Ethylene. However, the information about the most recent group of phytohormone (Brassinosteroids) has been incorporated in this book. This volume includes a selection of newly written, integrated, illustrated reviews describing our knowledge of Brassinosteroids and aims to describe them at the present time. Various chapters incorporate both theoretical and practical aspects and may serve as baseline information for future researches through which significant developments are possible. This book will be useful to the students, teachers and researchers, both in universities and research institutes, especially in relation to biological and agricultural sciences.

Advances in molecular biology and genome research in the form of molecular breeding and genetic engineering put forward innovative prospects for improving productivity of many pulses crops. Pathways have been discovered, which include regulatory elements that modulate stress responses (e.g., transcription factors and protein kinases) and functional genes, which guard the cells (e.g., enzymes for generating protective metabolites and proteins). In addition, numerous quantitative trait loci (QTLs) associated with elevated stress tolerance have been cloned, resulting in the detection of critical genes for stress tolerance. Together these networks can be used to enhance stress tolerance in pulses. This book summarizes recent advances in pulse research for increasing productivity, improving biotic and abiotic stress tolerance, and enhancing nutritional quality.

Petunia belongs to the family of the Solanaceae and is closely related to important crop species such as tomato, potato, eggplant, pepper and tobacco. With around 35 species described it is one of the smaller genera and among those there are two groups of species that make up the majority of them: the purple flowered P.integrifolia group and the white flowered P.axillaris group. It is assumed that interspecific hybrids between members of these two groups have laid the foundation for the huge variation in cultivars as selected from the 1830 s onwards.

Petunia thus has been a commercially important ornamental since the early days of horticulture. Despite that, Petunia was in use as a research model only parsimoniously until the late fifties of the last century. By then seed companies started to fund academic research, initially with the main aim to develop new color varieties. Besides a moment of glory around 1980 (being elected a promising model system, just prior to the Arabidopsis boom), Petunia has long been a system in the shadow. Up to the early eighties no more then five groups developed classical and biochemical genetics, almost exclusively on flower color genes. Then from the early eighties onward, interest has slowly been growing and nowadays some 20-25 academic groups around the world are using Petunia as their main model system for a variety of research purposes, while a number of smaller and larger companies are developing further new varieties.

At present the system is gaining credibility for a number of reasons, a very important one being that it is now generally realized that only comparative biology will reveal the real roots of evolutionary development of processes like pollination syndromes, floral development, scent emission, seed survival strategies and the like.

As a system to work with, Petunia combines advantages from several other model species: it is easy to grow, sets abundant seeds, while self- and cross pollination is easy; its lifecycle is four months from seed to seed; plants can be grown very densely, in 1 cm2 plugs and can be rescued easily upon flowering, which makes even huge selection plots easy to handle. Its flowers (and indeed leaves) are relatively large and thus obtaining biochemical samples is no problem. Moreover, transformation and regeneration from leaf disc or protoplast are long established and easy-to-perform procedures. On top of this easiness in culture, Petunia harbors an endogenous, very active transposable element system, which is being used to great advantage in both forward and reverse genetics screens.

The virtues of Petunia as a model system have only partly been highlighted. In a first monograph, edited by K. Sink and published in 1984, the emphasis was mainly on taxonomy, morphology, classical and biochemical genetics, cytogenetics, physiology and a number of topical subjects. At that time, little molecular data was available. Taking into account that that first monograph will be offered electronically as a supplement in this upcoming edition, we would like to put the overall emphasis for the second edition on molecular developments and on comparative issues.

To this end we propose the underneath set up, where chapters will be brief and topical. Each chapter will present the historical setting of its subject, the comparison with other systems (if available) and the unique progress as made in Petunia. We expect that the second edition of the Petunia monograph will draw a broad readership both in academia and industry and hope that it will contribute to a further expansion in research on this wonderful Solanaceae."

It was around 1970, I had just completed a 5-year breeding project aiming at fxing fower colour in gerbera progenies: white, yellow, pink, and red; colour homogeneity was sound, but size and shape still required some improvement. The problem was defnitely resolved by Murashige and Skoog, USA who published a reliable protocol for gerbera micropro- gation. In short, my gerbera seed lines were immediately rendered obsolete by this e- cient cloning system, able to produce millions of plants of a matchless and previously unknown homogeneity, the uniformity of fower shape and colour being the basic requi- ments for the market. The success of micropropagation resulted in a tremendous growth in gerbera fower production worldwide, and this species conquered a leading place in the foriculture industry. This personal experience stresses the impact of micropropagation on the genetic improvement research strategies in ornamentals. Micropropagation has become "in- sive", especially in ornamental plant material issues. Today, hundreds of protocols exist; however, only a modest percentage of them are exploited economically. Thus, only micropropagation of plants with a high market price range, like orchids for instance, has proved cost-effective and achieved great success. Micropropagation is a labour-intensive system: hand-power is estimated to rep- sent 60-70% of total costs. This explains the outsourcing of the major labs in developing countries where labour is cheaper. Nevertheless, certain industrial protocols remain a proprietary technology of leading labs, mostly western, with the exception of Japan and Taiwan.

Optical screening of excessive and potentially harmful solar radiation is an important photoprotective mechanism, though it has received much less attention in comparison with other systems preventing photooxidative damage to photoautotrophic organisms. This photoprotection in the form of screening appears to be especially important for juvenile and senescing plants as well as under environmental stresses-i.e. in situations where the efficiency of enzymatic ROS elimination, DNA repair and other classical' photoprotective systems could be impaired. This book represents an attempt to develop an integral view of optical screening-based photoprotection in microalgae and higher plants. Towards this end, the key groups of pigments involved in the screening of ultraviolet and visible components of solar radiation in microalgae and higher plants, and the patterns of their accumulation and distribution within plant cells and tissues, are described. Special attention is paid to the manifestations of screening pigment accumulation in the optical spectra of plants. It is also demonstrated that understanding these effects and their relationships to screening pigments' makeup and spectroscopy in plants provides valuable insights into the state of plants' long-term photoacclimation, as well as ample opportunities for the non-destructive quantification of screening pigments and the assessment of the efficiency of photoprotection providing by these pigments in situ.

In any ecosystem, plant and microbe interaction is inevitable. They not only co-exist but also support each other's survival and also provide for sustenance in stressful environment. Agro-ecosystems of many regions around the globe are affected by multi-stress. Major limiting factors affecting the agricultural productivity worldwide are environmental stresses. Apart from decreasing yield they introduce devastating impact on plant growth as well. Plants battle with various kind of stresses with the help of symbiotic association with the microbes in the rhizosphere. Naturally existing plant-microbe interaction facilitates survival of plants under these stressful conditions. Rhizosphere consists of many groups of microbes, plant growth-promoting bacteria (PGPB) is one such group of microbes which assist plants in coping with multiple stresses and in plant growth as well. These microbes help in stress physiology of the plants and can be extremely useful in solving agricultural as well food security problems. The proposed book is split into two parts, with an aim to provide comprehensive description and highlight a holistic approach. It elucidates various mechanisms in rhizosphere of nutrient management, stress tolerance and enhanced crop productivity. The book discusses rhizospheric flora and its importance in enhancement of plant growth, nutrient content, yield of various crops and vegetables as well as soil fertility and health. Both volumes of the book addresses fundamentals, applications as well as research trends and new prospects of agricultural sustainability. Volume 2: Nutrient Management and Crop Improvement, contains chapters which cover a broad overview of plant growth promoting activities of microbes. This proposed book also highlights the contribution of nitrogen, phosphorus, potassium, iron and zinc-solubilizing microbes from rhizospheric soil to develop efficient indigenous microbial consortia to enhance the food and nutritional security. With the given content and layout the proposed book will be an all-inclusive collection of information, which will be useful for students, academicians, researchers working in the field of rhizospheric mechanisms, agricultural microbiology, soil microbiology, biotechnology, agronomy and sustainable agriculture and also for policy makers in the area of food security and sustainable agriculture. It will be of special interest to both academics and professionals working in the fields of microbiology, soil microbiology, biotechnology and agronomy, as well as the plant protection sciences. Timely, this edited and research book provides an essential and comprehensive source of material from basic to advance findings on microbes and their role in agricultural and soil sustainability.

Chloroplasts are vital for life as we know it. At the leaf cell level, it is common knowledge that a chloroplast interacts with its surroundings - but this knowledge is often limited to the benefits of oxygenic photosynthesis and that chloroplasts provide reduced carbon, nitrogen and sulphur. This book presents the intricate interplay between chloroplasts and their immediate and more distant environments. The topic is explored in chapters covering aspects of evolution, the chloroplast/cytoplasm barrier, transport, division, motility and bidirectional signalling. Taken together, the contributed chapters provide an exciting insight into the complexity of how chloroplast functions are related to cellular and plant-level functions. The recent rapid advances in the presented research areas, largely made possible by the development of molecular techniques and genetic screens of an increasing number of plant model systems, make this interaction a topical issue.

Oxygen (O ) appeared in significant amounts in the Earth's atmosphere over 2. 2 2 billion years ago, largely due to the evolution of photosynthesis by cyanobacteria (Halliwell 2006). The O molecule is a free radical, as it has two impaired electrons 2 that have the same spin quantum number. This spin restriction makes O prefer to 2 accept its electrons one at a time, leading to the generation of the so-called reactive oxygen species (ROS). The chemical nature of these species dictates that they can create damage in cells. This has contributed to the creation of the "oxidative stress" concept; in this view, ROS are unavoidable toxic products of O metabolism and 2 aerobic organisms have evolved antioxidant defences to protect against this tox- ity (Halliwell 1981; Fridovich 1998). Indeed, even in present-day plants, which are full of antioxidants, much of the protein synthetic activity of chloroplasts is used to replace oxidatively damaged D1 and other proteins (Halliwell 2006). Yet, the use of the "oxidative stress" term implies that ROS exert their effects through indiscriminate widespread inactivation of cellular functions. In this context, ROS must not be able to react with lipids, proteins or nucleic acids in order to avoid any damage to vital cellular components. However, genetic evidence has suggested that, in planta, purely physicoche- cal damage may be more limited than previously thought (Foyer and Noctor 2005).

Changes in atmospheric carbon dioxide concentrations and global climate conditions have altered photosynthesis and plant respiration across both geologic and contemporary time scales. Understanding climate change effects on plant carbon dynamics is critical for predicting plant responses to future growing conditions. Furthermore, demand for biofuel, fibre and food production is rapidly increasing with the ever-expanding global human population, and our ability to meet these demands is exacerbated by climate change. This volume integrates physiological, ecological, and evolutionary perspectives on photosynthesis and respiration responses to climate change. We explore this topic in the context of modeling plant responses to climate, including physiological mechanisms that constrain carbon assimilation and the potential for plants to acclimate to rising carbon dioxide concentration, warming temperatures and drought. Additional chapters contrast climate change responses in natural and agricultural ecosystems, where differences in climate sensitivity between different photosynthetic pathways can influence community and ecosystem processes. Evolutionary studies over past and current time scales provide further insight into evolutionary changes in photosynthetic traits, the emergence of novel plant strategies, and the potential for rapid evolutionary responses to future climate conditions. Finally, we discuss novel approaches to engineering photosynthesis and photorespiration to improve plant productivity for the future. The overall goals for this volume are to highlight recent advances in photosynthesis and respiration research, and to identify key challenges to understanding and scaling plant physiological responses to climate change. The integrated perspectives and broad scope of research make this volume an excellent resource for both students and researchers in many areas of plant science, including plant physiology, ecology, evolution, climate change, and biotechnology. For this volume, 37 experts contributed chapters that span modeling, empirical, and applied research on photosynthesis and respiration responses to climate change. Authors represent the following seven countries: Australia (6); Canada (9), England (5), Germany (2), Spain (3), and the United States (12).

Plants possess a range of potential cellular mechanisms that may be involved in the detoxification of heavy metals and thus tolerance to metal stress. Metal toxicity causes multiple direct and indirect effects in plants that concern practically all physiological functions. The main purpose of this book is to present comprehensive and concise information on recent advances in the field of metal transport and how genetic diversity affects heavy metal transport in plants. Other key futures of the book are related to metal toxicity and detoxification mechanisms, biochemical tools for HM remediation processes, molecular mechanisms for HM detoxification, how metallomics and metalloproteomics are affected by heavy metal stress in plants, and the role of ROS metabolism in the alleviation of heavy metals. Some chapters also focus on recent developments in the field of phytoremediation. Overall the book presents in-depth information and the most essential advances in the field of heavy metal toxicity in plants in recent years.

This book covers many facets of plant selenium (Se) accumulation: molecular genetics, biochemistry, physiology, and ecological and evolutionary aspects. Broader impacts and applications of plant Se accumulation also receive attention. Plant Se accumulation is very relevant for environmental and human health. Selenium is both essential at low levels and toxic at high levels, and both Se deficiency and toxicity are problems worldwide. Selenium can positively affect crop productivity and nutritional value. Plants may also be used to clean up excess environmental Se. Selenium in plants has profound ecological impact, and likely contributes to Se movement in ecosystems and global Se cycling.

Volume 2 covers nitrogen fertilizer efficiency, acid tolerance of the legume symbiosis, fruit tree nutrition, rhizosphere pH change, iron deficiency in crop production, the effects of nutrient deficiences on seed production, the elemental composition of plants, and the role of potassium. The articles in this volume join together both the fundamental and the applied parts of this discipline. The editors' aim to make the reviews comprehensible to scientists in relevant disciplines, rather than purely to the specialist. The format of each volume is a small number of full-length reviews of important topics, plus an editorial which briefly mentions other rapidly developing topics that may therefore be reviewed in future volumes.

In spite of international agreements at the political level not much has changed since the late 1980s in terms of reducing the speed of destruction of original tropical environments.

However, since the publication of the first edition ten years ago, international research efforts in physiological ecology of plants in the tropics has increased enormously in quantity and quality. In some fields advances were more substantial than in others. New approaches came up in remote sensing and at the other end of the scope in some areas molecular biology was particularly developed regarding ecological performance of tropical plants, e.g. in understanding the adaptation of resurrection plants to the extreme habitat of inselbergs.

The wealth of new information made it necessary to break large chapters down into smaller ones. Tropical forests which occupy about half of the entire volume of the book were now arranged in 5 chapters covering structure and function under the influence of environmental cues and including epiphytes and mangroves as part of the tropical forest complex. Savannas were now treated in two chapters. Coastal salinas have been combined with a new section on the Brazilian restingas in a chapter on coastal sand plains.