This volume presents the proceedings of the Second International Sym posium on Genetic Aspects ofPlant Minerai Nutrition, held in Madison, Wisconsin in 1985. The mechanisms by which plants acquire, transport and utilize essential minerai nutrients are highly complex. The means by which plants either exclude or tolerate ions of metals toxic to plants are equally complex. The first symposium attempted to convene research scientists con cerned with minerai nutrition for the purpose of exploring the kinds of minerai nutrition phenomena identified as being under genetic contro!. The first symposium also placed much emphasis on research to which genetic intervention might be applied. At the second symposium more papers were presented on genetic and breeding research, a long-term objective of the first symposium. The second symposium also included biotic interactions under genetic con trol that either enhanced or impeded ion uptake, e.g. mycorrhizae and nitrogen fixing bacteria. This continuing dialogue is essential for a research area the complexity of which is due to its interdisciplinary nature.

Proceedings of a Seminar in the CEC Programme of the Coordination of Research on Plant Protein Improvement, held in Gembloux, Belgium, Sept. 3-5, 1985

A growing interest has been shown recently in the dymanics of nitrogen in agricultural and natural ecosystems. This has been caused by increasing demands for food and fibre by a rapidly expanding world population, and by a growing concern that increased land clearing, cultivation and use of both fertilizer and biologically fixed nitrogen can have detrimental effects on the environment. These include effects on water quality, eutrophication of surface waters and changes in atmospheric composition all caused by increased cycling of nitrogenous compounds. The input and availability of nitrogen frequently affects the productivity of farming systems more than any other single management factor, but often the nitrogen is used inefficiently. Much of the fertilizer nitrogen applied to the soil is not utilised by the crop: it is lost either in solution form, by leaching of nitrate, or in gaseous forms as ammonia, nitrous oxide, nitric oxide or dinitrogen. The leached nitrate can contaminate rivers and ground waters, while the emitted ammonia can contaminate surface waters or combine with atmospheric sulfur dioxide to form aerosols which affect visibility, health and climate. There is also concern that increased evolution of nitrous oxide will deplete the protective ozone layer of the stratosphere. The possibility of a link between the intensity of agricultural use of nitrogen, nitrous oxide emissions and amounts of stratospheric ozone has focussed attention on these interactions.

Branching morphogenesis, the creation of branched structures in the body, is a key feature of animal and plant development. This book brings together, for the first time, expert researchers working on a variety of branching systems to present a state-of-the-art view of the mechanisms that control branching morphogenesis. Systems considered range from single cells, to blood vessel and drainage duct systems to entire body plans, and approaches range from observation through experiment to detailed biophysical modelling. The result is an integrated overview of branching.

This book presents a detailed analysis of up-to-date literature on in vitro morphogenesis at cell, tissue, organ, and whole plant levels. Its driving force is the substantial advances made in the field of morphogenesis in tissue cultures during the last 25 years.

Progress in photosynthesis research is strongly dependent on instrumentation. It is therefore not surpr- ing that the impressive advances that have been made in recent decades are paralleled by equally impressive advances in sensitivity and sophistication of physical equipment and methods. This trend started already shortly after the war, in work by pioneers like Lou Duysens, the late Stacy French, Britton Chance, Horst Witt, George Feher and others, but it really gained momentum in the seventies and especially the eighties when pulsed lasers, pulsed EPR spectrometers and solid-state electronics acquired a more and more prominent role on the scene of scientific research. This book is different from most others because it focuses on the techniques rather than on the scientific questions involved. Its purpose is three-fold, and this purpose is reflected in each chapter: (i) to give the reader sufficient insight in the basic principles of a method to understand its applications (ii) to give information on the practical aspects of the method and (iii) to discuss some of the results obtained in photosynthesis research in order to provide insight in its potentalities. We hope that in this way the reader will obtain sufficient information for a critical assessment of the relevant literature, and, perhaps more important, will gain inspiration to tackle problems in his own field of research. The book is not intended to give a comprehensive review of photosynthesis, but nevertheless offers various views on the exciting developments that are going on.

This publication comprises the proceedings of the first International Conference devoted to the structural roots of trees and woody plants. 'The Supporting Roots - Structure and Function, ' 20-24 July 1998, Bordeaux, France. The meeting was held under the auspices ofIUFRO WPS 2. 01. 13 'Root Physiology and Symbiosis, ' and its aim was to bring together scientific researchers, foresters and arboriculturalists, to discuss current problems in structural root research and disseminate knowledge to an audience from a wide disciplinary background. For the first time in an international conference, emphasis was placed on presenting recent reseach in the field of tree anchorage mechanics and root biomechanics. The way in which tree stability can be affected by root system symmetry and architecture was addressed, as well as how movement during wind sway can influence the development and shape of woody roots. The role of different nursery and planting techniques was discussed, in relation to effects on root system form and development. Root response to different environmental stresses, including water, temperature, nutrient and mechanical stress was addressed in detail. The structure and function of woody roots was also considered at different levels, from coarse to fine roots, with several papers discussing the interaction between roots and the rhizosphere. One of the conference highlights was the presentation of new methods in root research, by a series of workshops held at LRBB-INRA, Pierroton, on the northern border of the Gascony forest.

Saline land is a resource capable of significant production. Recent advances in research in breeding for salt tolerance in wheat, biotechnology in rice, and selection and rehabilitation of salt-tolerant plants are of economic importance in arid/saline conditions.

This book gives some practical approaches for saline agriculture and afforestation, and describes examples of cultivating salt-tolerant/halophytic plants for commercial interest on salt-affected land or with highly salinized water in Australia, China, Central Asia, Egypt, Pakistan, and Russia. It also explores the possibilities of arid/saline agriculture and afforestation in UAE.

Environmental stresses represent the most limiting factors for agricultural productivity. Apart from biotic stress caused by plant pathogens, there are a number of abiotic stresses such as extremes in temperature, drought, salinity, heavy metals and radiation which all have detrimental effects on plant growth and yield. However, certain plant species and ecotypes have developed various mechanisms to adapt to such stress conditions. Recent advances in the understanding of these abiotic stress responses provided the impetus for compiling up-to-date reviews discussing all relevant topics in abiotic stress signaling of plants in a single volume. Topical reviews were prepared by selected experts and contain an introduction, discussion of the state of the art and important future tasks of the particular fields.

Adverse environmental factors can impose stress on plants and influence the expression of the full genetic potential for growth and reproduction. The capability of plants to develop plastic response reactions, to adapt to environmental stress situations, is unique in the biological world. A goal of the research described in this volume is to increase crop productivity, particular in regions where the environment imposes stress. An understanding of the principles involved in plant adaptation to environmental stress will enable optimisation of practices to improve agronomic production and minimise damaging environmental impact. The aim of this volume is to link the rapidly advancing and increasingly specialist field of molecular biology with plant physiology at the ecosystem level. The book includes chapters focused on some principle methods and a series of up-to-date review chapters on plant adaptation to a variety of specific stresses. The utilisation of newly available genome information is emphasised. Of particular importance is the desire to highlight the current potential of such approaches, and how diverse disciplines can interact and complement one another. The book is aimed at both the specialist and the advanced student.

We are facing global issues concerning environmental pollution and shortages of food, feed, phytomass (plant biomass) and natural resources, which will become more serious in the forthcoming decades. To solve these issues, immeasurable numbers of various plants and huge amounts of phytomass are required every year for food, feed and for the improvement of amenities, the environment and our quality of life. Increased phytomass is also required as alternative raw material for producing bio-energy, biodegradable plastics and many other plant-originated industrial products. Only by using phytomass as a reproducible energy source and raw material, instead of fossil fuels and atomic power, we can save natural resources and minimize environmental pollution. To increase phytomass globally, we need billions of quality transplants (small plants) to be grown yearly, in the field or in the greenhouse, under various environmental conditions. However, these high quality transplants can be produced only under carefully controlled, rather than variable environment al conditions. Recent research has shown that the closed transplant production system requires considerably small amounts of electricity, water, fertilizer, CO) and pesticide to produce value-added transplants as scheduled with minimum release of environmental pollutants and minimum loss of transplants. The closed or closed-type transplant production system is defined as a transplant production system covered with opaque walls with minimized or controlled ventilation rates, using artificial lighting. With this system, photoperiod, light intensity and quality, air temperature, humidity, CO) concentration and air current speed can be controlled as desired.

Lipids in Photosynthesis provides readers with a comprehensive view of the structure, function and genetics of lipids in plants, algae and bacteria, with special emphasis on the photosynthetic apparatus in thylakoid membranes. This volume includes the historical background of the field, as well as a full review of our current understanding of the structure and molecular organization of lipids and their role in the functions of photosynthetic membranes. The physical properties of membrane lipids in thylakoid membranes and their relationship to photosynthesis are also discussed. Other topics include the biosynthesis of glycerolipids and triglycerides; reconstitution of photosynthetic structures and activities with lipids; lipid-protein interactions in the import of proteins into chloroplasts; the development of thylakoid membranes as it relates to lipids; genetic engineering of the unsaturation of membrane glycerolipids, with a focus on the ability of the photosynthetic machinery to tolerate temperature stress; and the involvement of chloroplast lipids in the reactions of plants upon exposure to stress. This book is intended for a wide audience and should be of interest to advanced undergraduate and graduate students and to researchers active in the field, as well as to those scientists whose fields of specialization include the biochemistry, physiology, molecular biology, biophysics and biotechnology of membranes.

The research in this book covers papers on a great number of research projects on the responses of plants and crops of natural terrestrial ecosystems, of agro-ecosystems, and of aquatic ecosystems, to enhanced solar UV-B as a result of stratospheric ozone depletion. Some introductory chapters deal with general aspects of how plants respond to UV-B radiation. Photosynthetically Active Radiation (PAR) is a primary energy resource for terrestrial plants, necessary for plant growth. Inevitably terrestrial plants absorb UV-B when exposed to solar radiation. The spectral balance between PAR and UV-B is discussed in several chapters. The responses of plants and ecosystems from the Antarctic and Arctic to enhanced solar UV-B radiation as a consequence of the hole in the ozone layer are considered in some detail. In addition the papers in the book discuss the problem of how responses of plants to UV-B radiation interact with other environmental factors. The book is of great importance for those who are involved in global change topics: biologists, ecologists, earth scientists, agronomists, environmental scientists, and those who develop environmental policy.

Conifer Cold Hardiness provides an up-to-date synthesis by leading scientists in the study of the major physiological and environmental factors regulating cold hardiness of conifer tree species. This state-of-the-art reference comprehensively explains current understanding of conifer cold hardiness ranging from the gene to the globe and from the highly applied to the very basic. Topics addressed encompass cold hardiness from the perspectives of ecology, ecophysiology, acclimation and deacclimation, seedling production and reforestation, the impacts of biotic and abiotic factors, and methods for studying and analyzing cold hardiness. The content is relevant to geneticists, ecologists, stress physiologists, environmental and global change scientists, pathologists, advanced nursery and silvicultural practitioners, and graduate students involved in plant biology, plant physiology, horticulture and forestry with an interest in cold hardiness.

The NATO Advanced Research Workshop (ARW) on "Regulation of Enzymatic Systems Detoxifying Xenobiotics in Plants" intended to provide a forum to scientists from academia, industry, and govemment for discussing and critically assessing recent advances in the field of xenobiotic metabolism in plants and for identifying new directions for future research. Plants function in a chemical environment made up of nutrients and xenobiotics. Xenobiotics (foreign chemicals) are natural or synthetic compounds that can not be utilized by plants for energy-yielding metabolism. Plants may be exposed to xenobiotics either deliberately, due to their use as pesticides or accidentally, from industrial, agricultural, and other uses. Plants, like most other organisms, evolved a remarkable battery or metabolic reactions to defend themselves against the potentially toxic effects of xenobiotics. The main enzymatic reactions utilized by plants for xenobiotic detoxification include oxidation, reduction, hydrolysis and conjugation with glutathione, sugars (e.g., glucose), and amino acids. Eventually, xenobiotic conjugates are converted to insoluble bound residues or to secondary conjugates, which are deposited in the vacuole of plant cells.

Photosynthesis and the Environment examines how photosynthesis may be influenced by environmental changes. Structural and functional aspects of the photosynthetic apparatus are examined in the context of responses to environmental stimuli; particular attention being given to the processing of light energy by thylakoids, metabolic regulation, gas exchange and source-sink relations. The roles of developmental and genetic responses in determining photosynthetic performance are also considered. The complexity of the responses to environmental change is demonstrated by detailed analyses of the effects of specific environmental variables (light, temperature, water, CO2, ozone and UV-B) on photosynthetic performance. Where appropriate attention is given to recent developments in the techniques used for studying photosynthetic activities.

The book is intended for advanced undergraduate and graduate students and a wide range of scientists with research interests in environmental effects on photosynthesis and plant productivity.

Bioactive Carbohydrate Polymers is probably the first book dealing with the latest in the field of polysaccharides and related products and their biological activities, especially the immunological effects. The different chapters describe the structure and bioactivity of polysaccharides from plants used in traditional medicine in different parts of the world, especially China, Japan and Europe. The focus of the book is on immunologically active plant and seaweed polysaccharides, pharmacological activities of sulphated polysaccharides of animal and seaweed origin, and on possible activities of polysaccharides in our food. Methods for isolation and characterisation of the polymers with chemical and enzymatic methods is covered, as well as discussions on the different biological test-systems and the information they provide. This book will be useful to scientists and postgraduate students working with polysaccharides and their possible uses, and should be of interest for people working in the areas of chemistry, biology, pharmacy and medicine.

In this comprehensive and stimulating text and reference, the authors have succeeded in combining experimental data with current hypotheses and theories to explain the complex physiological functions of plants. For every student, teacher and researcher in the plant sciences it offers a solid basis for an in-depth understanding of the entire subject area, underpinning up-to-date research in plant physiology. The authors vividly explain current research by references to experiments, they cite original literature in figures and tables, and, at the end of each chapter, list recent references that are relevant for a deeper analysis of the topic. In addition, an abundance of detailed and informative illustrations complement the text.

Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.

The association between plants and wind that first comes to mind might be plant damage from a strong wind such as a typhoon or monsoon. The winds this book will 1 discuss, however, are not this strong at all, but rather are only 2 m.s. or weaker, like a breeze that gently blows over a farming area. Such a breeze, in fact, instills vitality into plants and increases their growth rates. This book is an attempt to explain these beneficial effects on plants from a field perspective. One fundamental process necessary for plant growth is photosynthesis. Since it is a photochemical reaction, this synthesis has been studied with emphasis on light. Yet to shed light on dry-matter or carbohydrate production by plants, it is indispensable to pursue research not only into the mechanism of photosynthesis but also into photosynthetic production itself. I have observed various phenomena occurring in the production field, and have thereby realized it necessary to recognize photosynthesis as a phenomenon that carbon dioxide (C0 ) in the air diffuses into chloroplasts in the leaves, and to study 2 which environmental factors promote C0 diffusion into the leaves. 2 In this book, I am going to describe the effects of the natural environment on photosynthetic production, placing focus on the leaf boundary layer as an environmental factor for plant production."

In a convenient, single-source reference, this book examines plant growth substances and their relationship to a wide range of physiological processes, ranging from seed germination through the death of the plant. If offers a clear illustration of the pragmatic uses of plant substances in agriculture and demonstrates how basic laboratory research has translated into increased production and profit for the grower. This work begins by building a solid foundation in the subject, which contains historical aspects and fundamental concepts, and provides a methodology for extraction, purification, and quantification of plant growth substances. This forms the basis for understanding the ensuing chapters that explore the many processes involving plant growth substances, including: * seed germination * seedling growth * rooting * dormancy * juvenility * maturity * senescence * flowering * abscission * fruit set * fruit growth * fruit development * premature drop * ripening * promotion of fruit drop * tuberization * photsynthesis * weed control. Providing a detailed examination of plant growth substances and their relationships to specific physiological plant processes, Plant Growth Substances gives students, researchers, and professionals a much needed reference.

Scientists within the field of plant biotechnology are in a constant search for techniques that can, in the simplest manner possible, answer the genetic and biochemical questions that underlie developmental processes. Thin Cell Layer Culture System not only takes an in-depth look at a technique that has had so much success in attempting, through various practical models and systems, to answer these issues, but also represents a celebration of almost 30 years of research that has covered a massive scope of plant species and areas of study. The initial studies conducted on tobacco thin cell layers (TCLs) - proving that organogenesis can be strictly controlled in vitro - allowed plant research to benefit from this finding, expanding this knowledge in a practical and applied manner into the biotechnological fields of tissue culture and micropropagation, cell and organ genetics and biochemistry.

The chapters in this book tell the enigmatic tale of TCLs. An historical perspective opens the scene for an inquiry into the possible cellular, biochemical and genetic processes that allow for the controlled development of a TCL into any organ type. The success of the system is further demonstrated in both monocotyledonous and dicotyledonous species, covering successful organogenesis and in vitro flowering in species within ornamental, leguminous and wood crops, cereals and grasses. Methodologies are outlined in detail, as is the rationale behind the TCL-organogenesis-developmental sequel. The TCL method, shown to be superior to many conventional micropropagation systems, has also shown to be vital in the recovery of transgenic plants.

This book is an essential part of every plant, cell and developmental biologist, geneticist and tissue culturalist's shelf as it addresses the primary issue of any plant: the cell, the tissue, and their subsequent development into a highly organized system.

This is a thorough study of photosynthetic mechanisms from cells to leaves, crown, and canopy. The authors question whether photosynthetic adaptations take place primarily at the metabolic and biochemical level or through changes in structure and form, or both. The text goes on to analyze the relative importance of genes that control metabolic and light reactions, and the structure, arrangement, and orientation of photosynthesis.

Each plant-pathogen interaction involves a two-way molecular communication. On one hand, the pathogen perceives signals from the plant, secretes chemical arsenals to establish infection courts, and produces metabolites that disrupt structural integrity, alter cellular function, and circumvent host defenses. On the other hand, the plant senses the signals from the pathogen, reinforces its cell walls, and accumulates phytoalexins and pathogenesis-related proteins in an attempt to defend itself. The production of pathogenicity and virulence factors by the pathogen, the elicitation of defense mechanisms by the plant, and the dynamic interaction of the two are the focal points of this book. The book will be of interest to researchers and advanced undergraduate and graduate students in the areas of plant pathology, plant physiology, and plant biochemistry.

A Personal Note I decided to initiate Orchid Biology: Reviews and Perspectives in about 1972 and (alone or with co-authors) started to write some of the chapters and the appendix for the volume in 1974 during a visit to the Bogor Botanical Gardens in Indonesia. Professor H. C. D. de Wit of Holland was also in Bogor at that time and when we discovered a joint interest in Rumphius he agreed to write a chapter about him. I visited Bangkok on my way home from Bogor and while there spent time with Professor Thavorn Vajrabhaya. He readily agreed to write a chapter. The rest of the chapters were solicited by mail and I had the complete manuscript on my desk in 1975. With that in hand I started to look for a publisher. Most of the publishers I contacted were not interested. Fortunately Mr James Twiggs, at that time editor of Cornell University Press, grew orchids and liked the idea. He decided to publish Orchid Biology: Reviews and Per spectives, and volume I saw the light of day in 1977. I did not know if there would be a volume II but collected manuscripts for it anyway. Fortunately volume I did well enough to justify a second book, and the series was born. It is still alive at present - 20 years, seven volumes and three publishers later. I was in the first third of my career when volume I was published."