Plant secondary metabolism is an economically important source of fine chemicals, such as drugs, insecticides, dyes, flavours, and fragrances. Moreover, important traits of plants such as taste, flavour, smell, colour, or resistance against pests and diseases are also related to secondary metabolites. The genetic modification of plants is feasible nowadays. What does the possibility of engineering plant secondary metabolite pathways mean? In this book, firstly a general introduction is given on plant secondary metabolism, followed by an overview of the possible approaches that could be used to alter secondary metabolite pathways. In a series of chapters from various authorities in the field, an overview is given of the state of the art for important groups of secondary metabolites. No books have been published on this topic so far. This book will thus be a unique source of information for all those involved with plants as chemical factories of fine chemicals and those involved with the quality of food and ornamental plants. It will be useful in teaching graduate courses in the field of metabolic engineering in plants.

Each plant-pathogen interaction involves a two-way molecular communication. On one hand, the pathogen perceives signals from the plant, secretes chemical arsenals to establish infection courts, and produces metabolites that disrupt structural integrity, alter cellular function, and circumvent host defenses. On the other hand, the plant senses the signals from the pathogen, reinforces its cell walls, and accumulates phytoalexins and pathogenesis-related proteins in an attempt to defend itself. The production of pathogenicity and virulence factors by the pathogen, the elicitation of defense mechanisms by the plant, and the dynamic interaction of the two are the focal points of this book. The book will be of interest to researchers and advanced undergraduate and graduate students in the areas of plant pathology, plant physiology, and plant biochemistry.

This is a thorough study of photosynthetic mechanisms from cells to leaves, crown, and canopy. The authors question whether photosynthetic adaptations take place primarily at the metabolic and biochemical level or through changes in structure and form, or both. The text goes on to analyze the relative importance of genes that control metabolic and light reactions, and the structure, arrangement, and orientation of photosynthesis.

Chloroplasts are vital for life as we know it. At the leaf cell level, it is common knowledge that a chloroplast interacts with its surroundings - but this knowledge is often limited to the benefits of oxygenic photosynthesis and that chloroplasts provide reduced carbon, nitrogen and sulphur. This book presents the intricate interplay between chloroplasts and their immediate and more distant environments. The topic is explored in chapters covering aspects of evolution, the chloroplast/cytoplasm barrier, transport, division, motility and bidirectional signalling. Taken together, the contributed chapters provide an exciting insight into the complexity of how chloroplast functions are related to cellular and plant-level functions. The recent rapid advances in the presented research areas, largely made possible by the development of molecular techniques and genetic screens of an increasing number of plant model systems, make this interaction a topical issue.

The Handbook of Plant Ecophysiology Techniques you have now in your hands is the result of several combined events and efforts. The birth of this handbook can be traced as far as 1997, when our Plant Ecophysiology lab at the University of Vigo hosted a practical course on Plant Ecophysiology Techniques. That course showed us how much useful a handbook presenting a bunch of techniques would be for the scientists beginning to work on Plant Ecophysiology. In fact, we wrote a short handbook explaining the basics of the techniques taught in that 1997 course: Flow cytometry to measure ploidy levels, Use of a Steady-State porometer to measure transpiration, In vivo measure of fluorescence, HPLC analysis of low molecular weight phenolics, Spectrophotometric determinations of free proline and soluble proteins, TLC polyamines contents measures, Isoenzymatic electrophoresis, Use of IRGA and oxygen electrode. That modest handbook, written in Spanish, was very helpful, both for the people who attended the course and for other who have used it for beginning to work in Plant Ecophysiology. The present Handbook is much more ambitious, and it includes more techniques. But we have also had in mind the young scientists beginning to work on Plant Ecophysiology. In 1999 Francois Pellissier leaded a proposal presented to the European Commission in the Fifth Framework Program in the High Level * Scientific Conferences, including three EuroLab Courses about lab and field techniques useful to improve allelopathic research."

In the two decades since the last comprehensive work on plant peroxisomes appeared, the scientific approaches employed in the study of plant biology have changed beyond all recognition. The accelerating pace of plant research in the post-genomic era is leading us to appreciate that peroxisomes have many important roles in plant cells, including reserve mobilisation, nitrogen assimilation, defence against stress, and metabolism of plant hormones, which are vital for productivity and normal plant development. Many plant scientists are finding, and will no doubt continue to find, that their own area of research is connected in some way to peroxisomes.

Written by the leading experts in the field, this book surveys peroxisomal metabolic pathways, protein targeting and biogenesis of the organelle and prospects for the manipulation of peroxisomal function for biotechnological purposes. It aims to draw together the current state of the art as a convenient starting point for anyone, student or researcher, who wishes to know about plant peroxisomes.

The 12th International Symposium on Plant Lipids was held at the University of Toronto, Canada, from July 7th to 12th, 1996. The conference was attended by over 200 scientists from university, government and corporate laboratories from 24 different countries. The topics covered in the symposium ranged from basic physiology, biochemistry and molecular biology of plant lipids to transformation and genetic engineering of crop plants. Oil seed plants were a particular focus of the symposium. There were 62 oral and 96 posters presentations. A special lecture in memory of the founder of this series of symposium, Terry Galliard, was presented by John Shanklin. This Proceedings Book has been dedicated to Grattan Roughan for his important contributions to our knowledge of plant lipid metabolism. This volume contains manuscripts submitted from most of the presentations at the symposium. It provides a useful summary of the major fields of plant lipid studies and our present state of knowledge. The papers are arranged in eight sections covering the major areas in the field of plant physiology, biochemistry and molecular biology of plant lipids. We would like to thank Valerie Imperial, Rajesh Khetarpal and Mary Williams for their invaluable help in organizing and running the meetings and excursions. John P. Williams, Mobashsher U. Khan and Nora W. Lem Toronto, Canada, October 1996 xvii DEDICATION This volume is dedicated to Grattan Roughan.

Scientists within the field of plant biotechnology are in a constant search for techniques that can, in the simplest manner possible, answer the genetic and biochemical questions that underlie developmental processes. Thin Cell Layer Culture System not only takes an in-depth look at a technique that has had so much success in attempting, through various practical models and systems, to answer these issues, but also represents a celebration of almost 30 years of research that has covered a massive scope of plant species and areas of study. The initial studies conducted on tobacco thin cell layers (TCLs) - proving that organogenesis can be strictly controlled in vitro - allowed plant research to benefit from this finding, expanding this knowledge in a practical and applied manner into the biotechnological fields of tissue culture and micropropagation, cell and organ genetics and biochemistry.

The chapters in this book tell the enigmatic tale of TCLs. An historical perspective opens the scene for an inquiry into the possible cellular, biochemical and genetic processes that allow for the controlled development of a TCL into any organ type. The success of the system is further demonstrated in both monocotyledonous and dicotyledonous species, covering successful organogenesis and in vitro flowering in species within ornamental, leguminous and wood crops, cereals and grasses. Methodologies are outlined in detail, as is the rationale behind the TCL-organogenesis-developmental sequel. The TCL method, shown to be superior to many conventional micropropagation systems, has also shown to be vital in the recovery of transgenic plants.

This book is an essential part of every plant, cell and developmental biologist, geneticist and tissue culturalist's shelf as it addresses the primary issue of any plant: the cell, the tissue, and their subsequent development into a highly organized system.

A Personal Note I decided to initiate Orchid Biology: Reviews and Perspectives in about 1972 and (alone or with co-authors) started to write some of the chapters and the appendix for the volume in 1974 during a visit to the Bogor Botanical Gardens in Indonesia. Professor H. C. D. de Wit of Holland was also in Bogor at that time and when we discovered a joint interest in Rumphius he agreed to write a chapter about him. I visited Bangkok on my way home from Bogor and while there spent time with Professor Thavorn Vajrabhaya. He readily agreed to write a chapter. The rest of the chapters were solicited by mail and I had the complete manuscript on my desk in 1975. With that in hand I started to look for a publisher. Most of the publishers I contacted were not interested. Fortunately Mr James Twiggs, at that time editor of Cornell University Press, grew orchids and liked the idea. He decided to publish Orchid Biology: Reviews and Per spectives, and volume I saw the light of day in 1977. I did not know if there would be a volume II but collected manuscripts for it anyway. Fortunately volume I did well enough to justify a second book, and the series was born. It is still alive at present - 20 years, seven volumes and three publishers later. I was in the first third of my career when volume I was published."

Jose and Gordon s Toward Agroforestry Design is an important reference for anyone interested in exploring or managing the physiological and ecological processes which underlie resource allocation and plant growth in agroforestry systems.

Drawing together a wide range of examples from around the world, the book highlights how recent developments in agroforestry research can contribute to understanding agroforestry system function, and discusses the potential application of agroforestry in addressing a range of land use challenges in both tropical and temperate regions of the world.

Including both original research and synthetic analyses, the book presents examples from a wide range of environments. It focuses largely on resource allocation both above- and below-ground including an assessment of the recent advances in analytical and modeling tools available to this work. Concluding with a synthesis of the key questions and research gaps highlighted throughout the text, the book will be particularly useful to students and researchers involved in natural resource management, agroforestry, and applied ecology."

From being to becoming important, myo-inositol and its derivatives including phosphoinositides and phosphoinositols involved in diversi?ed functions in wide varieties of cells overcoming its insigni?cant role had to wait more than a century. Myo-inositol, infact, is the oldest known inositol and it was isolated from muscle as early as 1850 and phytin (Inositol hexakis phosphate) from plants by Pfeffer in 1872. Since then, interest in inositols and their derivatives varied as the methodology of isolation and puri?cation of the stereoisomers of inositol and their derivatives advanced. Phosphoinositides were ?rst isolated from brain in 1949 by Folch and their structure was established in 1961 by Ballou and his coworkers. After the compilation of scattered publications on cyclitols by Posternak (1965), proceedings of the conference on cyclitols and phosphoinositides under the supervision of Hoffmann-Ostenhof, were p- lished in 1969. Similar proceedings of the second conference on the same s- ject edited by Wells and Eisenberg Jr was published in 1978. In that meeting at the concluding session Hawthorne remarked "persued deeply enough p- haps even myoinositol could be mirror to the whole universe." This is now infact the scenario on the research on inositol and their phosphoderivatives. Finally a comprehensive information covering the aspects of chemistry, b- chemistry and physiology of inositols and their phosphoderivatives in a book entitled Inositol Phosphates written by Cosgrove (1980) was available.

This richly illustrated book presents the most recent up-to-date knowledge on Clusia with a wealth of data in tables and figures and comprehensive referencing. Clusia 's unique features as well as its extreme flexibility have put it in the limelight of international research. Covering all aspects of tree biology, this richly illustrated volume is an invaluable source of information for any plant scientist.

The Forest Primary Production Research Group was born in the Department of S- viculture, University of Helsinki in the early 1970s. Intensive ?eld measurements of photosynthesis and growth of forest vegetation and use of dynamic models in the interpretation of the results were characteristic of the research in the group. Electric instrumentation was based on analogue techniques and the analysis of the obtained measurements was based on self-written programs. Joint research projects with the Research Group of Environmental Physics at the Department of Physics, lead by Taisto Raunemaa (1939-2006) started in the late 1970s. The two research groups shared the same quantitative methodology, which made the co-operation fruitful. Since 1980 until the collapse of the Soviet Union the Academy of Finland and the Soviet Academy of Sciences had a co-operation program which included our team. The research groups in Tartu, Estonia, lead by Juhan Ross (1925-2002) and in Petrozawodsk, lead by Leo Kaipiainen (1932-2004) were involved on the Soviet side. We had annual ?eld measuring campaigns in Finland and in Soviet Union and research seminars. The main emphasis was on developing forest growth models. The research of Chernobyl fallout started a new era in the co-operation between forest ecologists and physicists in Helsinki. The importance of material ?uxes was realized and introduced explicitly in the theoretical thinking and measurements.

Studies in floral biology are largely concerned with how flowers function to promote pollination and mating. The role of pollination in governing mating patterns in plant populations inextricably links the evolution of pollination and mating systems. Despite the close functional link between pollination and mating, research conducted for most of this century on these two fundamental aspects of plant reproduction has taken quite separate courses. This has resulted in suprisingly little cross-fertilization between the fields of pollination biology on the one hand and plant mating-system studies on the other. The separation of the two areas has largely resulted from the different backgrounds and approaches adopted by workers in these fields. Most pollination studies have been ecological in nature with a strong emphasis on field research and until recently few workers considered how the mechanics of pollen dispersal might influence mating patterns and individual plant fitness. In contrast, work on plant mating patterns has often been conducted in an ecological vacuum largely devoid of information on the environmental and demographic context in which mating occurs. Mating-system research has been dominated by population genetic and theoretical perspectives with surprisingly little consideration given to the proximate ecological factors responsible for causing a particular pattern of mating to occur.

All measurements of intact leaf 02 sensitivity can be explained by the oxygenation model for glycolate formation and glycolate metabolism by established pathways. Predicting the rate of oxygenation from the underlying biochemistry is more reliable than calculating the rate of oxygenation from intact leaf gas exchange measurements. REFERENCES 1. Badger MR, TD Sharkey, S von Caemmerer: The relationship between steady-state gas exchange of bean leaves and the levels of carbon reduction cycle intermediates. Planta 160:305-313, 1984. 2. Bowes, G, WL Ogren, RH Hageman: Phosphoglycolate production catalyzed by ribulose diphosphate carboxylase. Biochem. Biophys. Res. Commun. 45:716-722, 1971. 3. Farquhar GD, S von Caemmerer, JA Berry: A biochemical model of photosynthetic C02 assimilation in leaves of C3 species. Planta 149: 78-90, 1980. 4. Farquhar GD, S von Caemmerer: Modelling of photosynthetic response to environmental conditions. In OL Lange, PS Nobel, CB Osmond, H Ziegler, eds, Encycl. of Plant Physiol., New Series, Springer Verlag, Heidelberg 12b: 549-587, 1982. 5. Jordan DB, WL Ogren: The C02/02 specificity of ribulose 1- bisphosphate carboxylase/oxygenase. Dependence on ribulose bisphosphate concentration, pH and temperature. Planta 161: 308-313, 1984. 6. Ku SB, GE Edwards: Oxygen inhibition of photosynthesis. I. Temperature dependence and relation to 02/C02 solubility ratio. Plant Physiol 59: 986-990, 1977. 7. Laing WA, WL Ogren, RL Hageman: Regulation of soybean net photosynthetic C02 fixation by the interaction of C02' 02 and ribulose l,5-diphosphate carboxylase. Plant Physiol 54: 678-685, 1974."

Phosphorus (P) is an essential macronutrient for plant growth. It is as phosphate that plants take up P from the soil solution. Since little phosphate is available to plants in most soils, plants have evolved a range of mechanisms to acquire and use P efficiently - including the development of symbiotic relationships that help them access sources of phosphorus beyond the plant's own range. At the same time, in agricultural systems, applications of inorganic phosphate fertilizers aimed at overcoming phosphate limitation are unsustainable and can cause pollution. This latest volume in Springer's Plant Ecophysiology series takes an in-depth look at these diverse plant-phosphorus interactions in natural and agricultural environments, presenting a series of critical reviews on the current status of research. In particular, the book presents a wealth of information on the genetic and phenotypic variation in natural plant ecosystems adapted to low P availability, which could be of particular relevance to developing new crop varieties with enhanced abilities to grow under P-limiting conditions. The book provides a valuable reference material for graduates and research scientists working in the field of plant-phosphorus interactions, as well as for those working in plant breeding and sustainable agricultural development.

With one volume each year, this series keeps scientists and advanced students informed of the latest developments and results in all areas of the plant sciences. The present volume includes reviews on genetics, cell biology, physiology, comparative morphology, systematics, ecology, and vegetation science.

Nearly twenty years have passed since the publishing of a broadly-based texthook on hiolog ical control (Huffaker and Messinger 1976). In the interim, other works on biological control have been either briefer treatments (DeBach and Rosen 1991), or collections of essays on selected topics (Waage and Greathead 1986; Mackauer and Ehler 1990). Our text has been written to fill what we believe is a need for a well-integrated, broadly-based text of appropriate length and degree of technical detail for teaching a one semester upper level course in hiological control. We have attempted to focus on principles and concepts, rather than on biological control of particular taxa or hiological control by particular kinds of natural enemies. Therefore, for example, the reader will find the material on biological control of weeds integrated with biological control of insects and mites into chapters on principles, techniques, and applications rather than presented separately. Only biological control of plant pathogens is addressed independently, an appraoch made necessary by the many special features and concepts in plant pathogen biological control."

Sulfur is one of the most versatile elements in life. This book provides, for the first time, in-depth and integrated coverage of the functions of sulfur in phototrophic organisms including bacteria, plants and algae. It bridges gaps between biochemistry and cellular biology of sulfur in these organisms, and of biology and environments dominated by them. The book therefore provides a comprehensive overview of plant sulfur relations from genome to environment.

This book provides an excellent illustration of the interrelationship between progress in scientific methodology and conceptual advances, and its publica tion should contribute to further advances. It is well known that major advances in understanding often follow the development of new methods. The development of the acetylene reduction assay for nitrogenase activity provides a good example of this interrelationship between theory and methods. Theoretical knowledge led to a search for substrates for nitro genase that could be assayed for more easily than ammonium, the normal product of the enzyme. The discovery of the reduction of acetylene to ethylene by nitrogenase provided the ideal answer to the problem by provid ing a rapid, specific, nondestructive, and inexpensive assay for nitrogenase activity. This assay is now used by almost every laboratory doing research on nitrogen fixation. However, further use and development of the acetylene reduction assay has shown that it can underestimate nitrogenase activity and can even give incorrect relative values under some circumstances. The major problem is that exposure of legume nodules to acetylene can cause a large increase in the resistance to oxygen diffusion into the nodule. This reduced supply of oxygen decreases the rate of nitrogenase activity within a few minutes."

Rinie Hofstra has been a member of the Department of Plant Physiology, University of Groningen, the Netherlands, for 24 years. The nearer we came to 31 March 1985, her 65th birthday, the more we all realized how we would miss her - not only scientifically, but also socially. She left her mark on both research and teaching, always with an open mind and willing to change. After her PhD Thesis on 'Nitrogen Metabolism in Tomato Plants' she first continued working in that field, but soon started a joint project with the Department of Plant Ecology on hemiparasites. She then became involved in carbon metabolism, which resulted in her giving a Biotrop Course on C /C metabolism in 3 4 Indonesia. Her own research group, originally working on 'Nitrogen Metabolism', soon embraced 'Energy and Nitrogen Metabolism', as the research on respiration became more and more important. In running her group she showed all sides of her person. She used to stimulate and encourage everyone around her and to integrate the various lines of research. At the same time she always had an open mind for the opinion of all members of her group. And together they regularly criticized and evaluated the various projects and decided how to continue.

The Annual Beltsville Symposium provides a forum for interaction among scientists involved in research that is vitally important to agri culture and to the agricultural sciences. The Twelfth Symposium in this series focused on the unifying biochemical and physiological mechan isms controlling growth and development of biological systems - ani mals, plants insects. Unraveling the complex biochemical mechanisms associated with the sequencing of organism growth and development and identifying, locating, and manipulating key control mechanisms are essential in utilizing the full potential of biotechnology for improving the composition and quality of agricultural products and the profitability of agriculture. Accordingly, speakers directed their remarks to basic aspects of biological mechanisms in their area of specialization with considera tion given to current status, future direction, potential impact, and limitations to progress. The Symposium addressed fundamental questions in: -Tissue specific gene regulation: cell division and differentiation -Mechanisms for regulating hormone concentration -Hormonal regulation of growth and development -Non-hormonal regulation of growth and development -Nutritional regulation of growth and development Because the backgrounds of the symposium attendees covered a wide spectrum in the basic biological and physical sciences, each topic was introduced by a brief overview, but general reviews were avoided in favor of findings from on-going research projects. The symposium brought together a distinguished group of invited scientists from around the world who are leaders. Many attendees made poster presentations which increased the exchange of ideas and sti mulated informal discussion."

Studies on the phenomenon of plant pathogenesis (disease development) have been useful to have a deep insight into the interactions between host plant and the pathogen. Depending on the levels of susceptibility (compatibility) or resistance (incompatibility) of the host plant and virulence of the pathogen, disease development may progress, either leading to symptom expression or result in the suppression of pathogen proliferation. Molecular techniques have been applied to elucidate the nature of interactions between the gene products of the plant and pathogen at cellular and molecular levels. Successful evasion of host's surveillance system and subsequent activities of metabolites of the pathogen (enzymes and toxins) encoded by pathogen genes counteracting the effects of various defense-related antimicrobial compounds present already or produced by the host plants, after initiation of infection have been critically studied by applying various molecular techniques. In addition to studying various phases of disease development in individual plants, molecular methods have been demonstrated to be effective, in gathering data on various aspects of epidemiology under natural conditions where the interaction of pathogen with populations of plants is influenced significantly by the environmental conditions existing in different ecosystems. This volume focuses on the possibility of applying the knowledge on pathogenesis and molecular epidemiology to determine the vulnerable stages in the life cycles of the pathogens that can be disrupted to achieve more effective disease control.

This book summarizes the experimental work conducted during a trans-disciplinary research program conducted for six years by the German Research Foundation. Each chapter includes introductory remarks written by internationally recognized scientists in their research areas.

Contributiing authors representing outstanding German scientists from such different disciplines as Physics, Biochemistry, Plant Nutrition, Botany, and Molecular Biology not only report original research but also review the state of knowledge in their fields of research.

The importance of mycorrhiza for the improvement of plant growth is increasingly being realised in Agriculture and Forestry and several mycorrhizal fungi have been commercially recognised for the purpose. The aim of this book is to describe the various techniques used to study the mycorrhizal biology. Problems with preparing such a book are many. Mainly mailing of manuscripts to and from authors resulted in irregular and final editing. Every effort was made not to change the peer review original manuscript to ensure accuracy. Our sole aims is to communicate to the greatest extent possible a current world need in mycorrhizal research. Plant productivity is regulated by microbial associations established in the plant root systems. The interactions of microorganisms and plant roots are especially important in providing nutritional requirements of the plant and the associated microorganisms. Plant growth and development are controlled largely by the soil environment in the root region -rhizosphere. This is a very complex environment in which the effects of the plant on soil microorganisms and the effects of microorganisms on the plant are interacting, interdependent and highly complex. Plant root exudates and breakdown products feed the microbes and the microbe in tum often benefit the plant. Mycorrhizal fungi are important tools for increasing growth, development and yield of economically important plants, they play important role of biofertilizer which can help establish plants in nutrient deficient soils, particularly phosphorus deficient soils, arid, semi-arid and waste lands.