This book discusses cancers and the resurgence of public interest in plant-based and herbal drugs. It also describes ways of obtaining anti-cancer drugs from plants and improving their production using biotechnological techniques. It presents methods such as cell culture, shoot and root culture, hairy root culture, purification of plant raw materials, genetic engineering, optimization of culture conditions as well as metabolic engineering with examples of successes like taxol, shikonin, ingenol mebutate and podophylotoxin. In addition, it describes the applications and limitations of large-scale production of anti-cancer compounds using biotechnological means. Lastly, it discusses future economical and eco-friendly strategies for obtaining anti-cancer compounds using biotechnology.

This book provides an overview of the latest advancements in the field of alien introgression in wheat. The discovery and wide application of molecular genetic techniques including molecular markers, in situ hybridization, and genomics has led to a surge in interspecific and intergeneric hybridization in recent decades. The work begins with the taxonomy of cereals, especially of those species which are potential gene sources for wheat improvement. The text then goes on to cover the origin of wheat, breeding in connection with alien introgressions, and the problems of producing intergeneric hybrids and backcross derivatives. These problems can include crossability, sterility, and unequal chromosome transmission. The work then covers alien introgressions according to the related species used, as well as new results in the field of genomics of wild wheat relatives and introgressions.

A range of techniques is available to the plant breeder today to complement classical breeding methods. The options are based on the integration of advances in plant cell biology with those in plant molecular biology. Plant cell, tissue and organ cultures provide efficient systems for transformation, for the achievement of wide crosses and for the production of variation through spontaneous and induced mutation, while permitting effective isolation of desired genotypes by in vitro selection. This volume presents a critical appraisal of the methodologies of plant genetic manipulation for advanced undergraduates, postgraduates, researchers and plant breeders, and provides guidance on the choice of breeding options. The latter depends on the breeding system of the crop, the breeding objective and the tissue culture systems applicable to the target genotype(s).

The population of the world continues to increase at an alarming rate. The trouble linked with overpopulation ranges from food and water scarcity to inadequacy of space for organisms. Overpopulation is also linked with several other demographic hazards, for instance, population blooming will not only result in exhaustion of natural repositories, but it will also induce intense pressure on the world economy. Today nanotechnology is often discussed as a key discipline of research but it has positive and negative aspects. Also, due to industrialization and ever-increasing population, nano-pollution has been an emerging topic among scientists for investigation and debate. Nanotechnology measures any substance on a macromolecular scale, molecular scale, and even atomic scale. More importantly, nanotechnology deals with the manipulation and control of any matter at the dimension of a single nanometer. Nanotechnology and nanoparticles (NPs) play important roles in sustainable development and environmental challenges as well. NPs possess both harmful and beneficial effects on the environment and its harboring components, such as microbes, plants, and humans. There are many beneficial impacts exerted by nanoparticles, however, including their role in the management of waste water and soil treatment, cosmetics, food packaging, agriculture, biomedicines, pharmaceuticals, renewable energies, and environmental remedies. Conversely, NPs also show some toxic effects on microbes, plants, as well as human beings. It has been reported that use of nanotechnological products leads to the more accumulation of NPs in soil and aquatic ecosystems, which may be detrimental for living organisms. Further, toxic effects of NPs on microbes, invertebrates, and aquatic organisms including algae, has been measured. Scientists have also reported on the negative impact of NPs on plants by discussing the delivery of NPs in plants. Additionally, scientists have also showed that NPs interact with plant cells, which results in alterations in growth, biological function, gene expression, and development. Thus, there has been much investigated and reported on NPs and plant interactions in the last decade. This book discusses the most recent work on NPs and plant interaction, which should be useful for scientists working in nanotechnology across a wide variety of disciplines.

Soil salinity is a key abiotic-stress and poses serious threats to crop yields and quality of produce. Owing to the underlying complexity, conventional breeding programs have met with limited success. Even genetic engineering approaches, via transferring/overexpressing a single 'direct action gene' per event did not yield optimal results. Nevertheless, the biotechnological advents in last decade coupled with the availability of genomic sequences of major crops and model plants have opened new vistas for understanding salinity-responses and improving salinity tolerance in important glycophytic crops. Our goal is to summarize these findings for those who wish to understand and target the molecular mechanisms for producing salt-tolerant and high-yielding crops. Through this 2-volume book series, we critically assess the potential venues for imparting salt stress tolerance to major crops in the post-genomic era. Accordingly, perspectives on improving crop salinity tolerance by targeting the sensory, ion-transport and signaling mechanisms are presented here in volume 1. Volume 2 will focus on the potency of post-genomic era tools that include RNAi, genomic intervention, genome editing and systems biology approaches for producing salt tolerant crops.

This book is designed to popularize Quinoa cereal among both scientific and food industry. Quinoa is an attractive candidate for protein replacement, has potential for futuristic biotechnological modifications, and is able to grow under many different abiotic stresses. To save the world from animal cruelty, quinoa emerges as a hero for vegans and vegetarians. This book deals with morphological features, life cycle, nutritional qualities, genetics, agronomic manipulations, ecological communications, stress tolerance mechanisms, and food applications of Chenopodium quinoa. Quinoa is a pseudo-cereal native to Andes Region in South America. Over time, it spread to many different regions worldwide and is emerging as protein-rich vegetarian food source. In order to cure malnutrition globally, it is important to channel this lesser-known grain to local cultivators. This can only be done through well-proven scientific data that supports its qualities. This book aims to do the same, while also giving an insight into the vast scope quinoa posses as an experimental crop. Its stress-tolerant abilities can inspire scientists to understand those mechanisms, further exploit them, and even introduce them into other stress-sensitive crops. In future, quinoa can be among the top sources that offer food security. Due to its adaptability, ease of cultivation, and rich output, sustainability can be achieved by regulating its breeding and growth. This book is of interest to researchers, teachers, agronomic cultivators, environmentalists, botanists, microbiologists, geneticists and food technologists. This book covers recent advances, challenges in cultivation, biology, nutrition, and agricultural science topics, suitable for both young learners and advanced scientists. Cultivators who want to know more about quinoa and introduce it into their agronomic applications will find helpful information from the text.

Bananas and plantains are among the most important food and cash crops in the world. They are cultivated in more than 135 countries, across the tropics and subtropics, with an annual global production of ca. 130 million metric tonnes. Though bananas are one of the most important components of food security in many developing countries, banana production is threatened by both abiotic and biotic stresses. These include a wide range of diseases and pests, such as bunchy top virus, burrowing nematodes, black Sigatoka or black leaf streak, Fusarium wilt, etc. In recent years, considerable progress has been made and several biotechnological and genomic tools have been employed to help understand and unravel the mysterious banana genome. Molecular and genomic studies have helped to decipher the Musa genome and its evolution. Genetic linkage map and whole genome sequencing of both Musa acuminata and Musa balbisiana (progenitors of cultivated banana) have completely changed the way of thinking and the approach on banana crop improvement. Whole-genome sequencing has helped to improve the selection of quantitative traits such as yield, as well as the selection of optimal parents for developing required hybrids in breeding programs. Gene isolation and the analysis of mutants have helped in the characterization of genes of agronomic value and the associated regulatory sequences. With the advent of molecular markers and new statistical tools, it is now possible to measure the diversity, identify genes and useful alleles linked to important agronomic traits. Further these alleles can be incorporated into cultivars through marker assisted selection or through transgenic approach. Transgenic approaches are potential tools for direct transfer of these genes into popular cultivars, which are generally not amenable for conventional breeding techniques, in specific with crops such as bananas which are sterile, triploid and heterozygous thereby making it difficult to reconstruct the recurrent genotypes in banana. Transgenic techniques thus have helped overcome the difficulty of working with sterile, triploid banana crop. In the last five years, enormous amount of new information and techniques have been generated for banana. A comprehensive book entitled "Banana: Genomics and Transgenic Approaches for Genetic improvement" on banana genomics, latest transgenic technologies and tools available for improved crop development in banana will address all these requirements.

This book compiles the latest applications of the cutting-edge gene editing tool CRISPR/Cas in the area of crop improvement. It begins with an introduction to the technique and its application in crop plants. Next, it gives an updated overview of available delivery methods, design tools and resources in CRISPR/Cas. The book subsequently reviews the applications of CRISPR/Cas in connection with e.g. insect stress, disease stress, abiotic stress, nutritional and yield improvement in crop plants, etc. It also discusses the various regulatory, ethical and social aspects of the technique that must be kept in mind when designing experiments. In closing, the book summarizes the status quo and outlines future prospects for the tool in crop improvement and food security. Given its scope, the book will especially benefit students and researchers in food science, biotechnology, agriculture and the plant sciences.

This book reviews the current state of knowledge concerning cacao pathogens and methods for their management. Topics discussed include the history, biology and genetic diversity of Moniliophthora species (which cause witches' broom and frosty pod rot) and Phytophthora species (which cause black pod rot) that cause diseases resulting in major losses to cacao production. Emerging pathogens such as Cacao swollen shoot virus and Ceratobasidium theobromae (which causes vascular streak dieback) are also discussed in detail, along with many pathogens of significant local concern. Most of these pathogens represent major risks to global cacao production should they expand into new areas, breaking out of their current limited distributions. By considering cacao diseases as a group, similarities in the available tools and techniques used in their management become apparent, as do their limitations. Gaps in our current knowledge of cacao pathogens and the management of the diseases they cause are detailed, and suggestions for future research directions are provided. This insight allows readers to consider cacao disease threats from a more comprehensive, global perspective and paves the way for an improved synergy of efforts between the various research programs, agencies, and industries, both private and public, with vested interests in cacao production, and cacao farmers.

This book provides all aspects of the physiology, stress responses and tolerance to abiotic stresses of the Brassicaceae plants. Different plant families have been providing food, fodder, fuel, medicine and other basic needs for the human and animal since the ancient time. Among the plant families, Brassicaceae has special importance for their agri-horticultural importance and multifarious uses apart from the basic needs. Interest understanding the response of Brassicaceae plants toward abiotic stresses is growing considering the economic importance and the special adaptive mechanisms. The knowledge needs to be translated into improved elite lines that can contribute to achieve food security. The physiological and molecular mechanisms acting on Brassicaceae introduced in this book are useful to students and researchers working on biology, physiology, environmental interactions and biotechnology of Brassicaceae plants.

This book aims to help plant breeders by reviewing past achievements, currently successful practices, and emerging methods and techniques. Theoretical considerations are also presented to strike the right balance between being as simple as possible but as complex as necessary. The United Nations predicts that the global human population will continue rising to 9.0 billion by 2050. World food production will need to increase between 70-100 per cent in just 40 years. First generation bio-fuels are also using crops and cropland to produce energy rather than food. In addition, land area used for agriculture may remain static or even decrease as a result of degradation and climate change, despite more land being theoretically available, unless crops can be bred which tolerate associated abiotic stresses. Lastly, it is unlikely that steps can be taken to mitigate all of the climate change predicted to occur by 2050, and beyond, and hence adaptation of farming systems and crop production will be required to reduce predicted negative effects on yields that will occur without crop adaptation. Substantial progress will therefore be required in bridging the yield gap between what is currently achieved per unit of land and what should be possible in future, with the best farming methods and best storage and transportation of food, given the availability of suitably adapted cultivars, including adaptation to climate change. My book is divided into four parts: Part I is an historical introduction; Part II deals with the origin of genetic variation by mutation and recombination of DNA; Part III explains how the mating system of a crop species determines the genetic structure of its landraces; Part IV considers the three complementary options for future progress: use of sexual reproduction in further conventional breeding, base broadening and introgression; mutation breeding; and genetically modified crops.

This book provides the latest information about hairy root culture and its several applications, with special emphasis on potential of hairy roots for the production of bioactive compounds. Due to high growth rate as well as biochemical and genetic stability, it is possible to study the metabolic pathways related to production of bioactive compounds using hairy root culture. Chapters discuss the feasibility of hairy roots for plant derived natural compounds. Advantages and difficulties of hairy roots for up-scaling studies in bioreactors are included as well as successful examples of hairy root culture of plant species producing bioactive compounds used in food, flavors and pharmaceutical industry. This book is a valuable resource for researchers and students working on the area of plant natural products, phytochemistry, plant tissue culture, medicines, and drug discovery.

The knowledge of ecology and epidemiology of rhizomania is particularly useful to understand the means and practices able to limit or avoid its further diffusion. Some promising methods of biological control using coexisting and non-pathogenic organisms could potentially help improve the action of the not completely effective genetic resistances. This integrated protection would be valuable, especially in the even more frequent development of resistance-breaking strains in the BNYVV, where the known types of resistance, alone or in combination, seem to have lost part of their original ability to protect the crop. Therefore, further efforts will be needed to discover new traits likely still present in the wild species of the genus Beta. The availability of large collections of germplasm stored in the International Beta gene-banks should ensure the enhanced efficiency of genetic resistance by means of conventional and marker-assisted selection methods. Some almost immune transgenic varieties seem already to be waiting for release where and when it will be possible. The introduction chapter describes briefly the sugar beet crop, the more common diseases, and the damage caused by rhizomania. The following chapters discuss biological properties of the causal virus, BNYVV, and its vector, Polymyxa betae, and their interactions with the environment and the host-plant. In particular, the great advances in research of the molecular biology of BNYVV should be noteworthy, which have been established by a wide range of the most modern methods. Recent work focused on the genetic diversity and evolution of BNYVV is moving forward our understanding of the dramatic worldwide epidemics of rhizomania. Newly developed molecular techniques also lead to practical applications, such as quantification of inoculum in ecological and epidemiological research.

This edited book provides a comprehensive overview of modern strategies in fruit crop breeding in the era of climate change and global warming. It demonstrates how advances in plant molecular and genomics-assisted breeding can be utilized to produce improved fruit crops with climate-smart traits. Agriculture is facing a number of challenges in the 21st century, as it has to address food, nutritional, energy and environmental security. Future fruit varieties must be adaptive to the varying scenarios of climate change, produce higher yields of high-quality food, feed, and fuel and have multiple uses. To achieve these goals, it is imperative to employ modern tools of molecular breeding, genetic engineering and genomics for 'precise' plant breeding to produce 'designed' fruit crop varieties. This book is of interest to scientists working in the fields of plant genetics, genomics, breeding, biotechnology, and in the disciplines of agronomy and horticulture.

This book, now in its second edition, provides researchers and operators a complete description of all aspects regarding the wild ancestor of sugar beet. The possibility of crossing modern crops with the ancestors from which they are derived in order to recover some traits lost through domestication is increasingly attracting interest. The selective process implemented by the first growers led to the elimination of features not considered useful at the time. Yet some of these lost traits have now become very important. In fact, in many areas sugar beet cultivation would now be impossible without the transfer of some genetic resistances from Beta maritima, the crop's ancestor. Moreover, the isolation of such traits is becoming increasingly critical with regard to current and future environmental and economic considerations on e.g. the use of pesticides. This second edition replaces certain photographs and has been updated to reflect the latest advances and findings. One chapter and several sections have been rewritten, and significant revisions have been made throughout the text. The new techniques provide breeders with massively improved analytical means for the safest and fastest selection procedures. Not only will these techniques allow Beta maritima to take on a far greater role as a source of favorable traits; the relative ease with which these characteristics can be transferred will also make it possible to use the germplasm of the whole genus Beta and Patellifolia, which to date has been highly complex, if not impossible, due to the difficulties of hybridization.

The genome is more than a linear code as depicted by its DNA sequences as several interacting factors play a crucial role in shaping its organization and function. The complete sequences of a number of plant genomes and the recent advances of high-throughput technologies has fueled research efforts in the field of Plant Nuclear Biology unveiling numerous insights about the mechanisms underlying genome regulation. Genomic information is being integrated into molecular- and cellular-level mechanisms of the plant processes. A host of nuclear processes underlie key developmental processes as well as biotic and abiotic interactions. Non-coding RNAs have been increasingly recognized as players in gene expression and genome defense and integrity. However, in vivo, genomes exist as elaborate physical structures, and their functional properties are strongly determined by their cellular organization. Various types of subcellular structure have been identified in the nucleus, which are associated with transcription factors, RNA processing proteins and epigenetic regulators. Interestingly, these nuclear bodies display different behaviors in response to the environment. This book compiles a series of landmark discussions of the recent advances in plant nuclear biology research focusing in the functional relevance of the arrangement of genomes and nuclear processes that impact plant physiology and development.

White rust caused by the fungus Albugo is the most devastating disease known to occur in more than 50 countries and infects about 400 plant species belonging to 31 families worldwide including important vegetable crucifers, oil yielding Brassicas, ornamental plants and numerous weeds. This book on "White Rust" deals with the aspects on "the disease" and "the pathogen" is vividly illustrated for stimulating, effective and easy reading and understanding. We are sure that this comprehensive treatise on "white rust" will be of immense use to the researchers, teachers, students and all others who are interested in the diagnosis and management of white rust diseases of crops worldwide.

This volume continues the series of books on "Plant Pathology in the 21st Century", and contains the papers given at the 10th International Congress of Plant Pathology (ICPP 2013) held in Beijing, August 25-30, 2013 concerning seed health. Many pathogens are transmitted throughout infected seeds and propagation material .The fact that propagation material production is very much concentrated in few establishments, favors the quick spread of new diseases throughout seed commercialization. This phenomenon is very much accelerated in a globalized system. The book covers case studies of contamination, aspects of detection and diagnosis as well as disease management strategies, with special emphasis towards seed treatments with unconventional products. This book will be useful for all plant pathologists as well as students in advanced courses.

Since the publication of "The coconut palm - A monograph" in 1960, considerable information has been accrued on the crop through work at research institutes, international organisations and development agencies. Although coconut cultivation is spread over 93 countries, providing employment and creating livelihood opportunities to 64 million families around the globe, smallholder coconut farmers are now facing numerous challenges. The wide gap between the potential and actual yield is a major concern, and as such it is necessary to disseminate knowledge in order to implement research findings. Coconut research in India, one of the leading coconut producing countries, is celebrating its centenary, making this an opportune time to review the research and development advances and the relevant technologies. This detailed, comprehensive book covers all aspects of coconut, from the origins to cultivation, breeding, physiology and value addition, as well as subjects of topical interest like nutrition and health, biotechnology, and climate change and carbon sequestration. Written by leading experts in the fields it emphasises that the livelihood of the small coconut landholders is the ultimate aim of scientists and developmental agencies, and outlines various important strategies to make coconut farming more remunerative globally. It discusses work in all the major coconut growing countries and outlines suggestions for international cooperation. Research work on the crop is comparatively difficult because of its perennial nature, longevity, height, long juvenile phase, large sized nuts, cross pollination and seed propagation. As these special features necessitate greater investment of resources, time and land, it is all the more imperative that research is not duplicated and the information and experience becoming available around the world is shared so that it can be fully utilised. In this context periodic publications, compiling all the available information on coconut assume greater significance. This book is therefore of great value to researchers, students, extension workers, developmental agencies and progressive farmers.

This book discusses and addresses the rapidly increasing world population demand for food, which is expected to double by 2050. To meet these demands farmers will need to improve crop productivity, which relies heavily on nitrogen (N) fertilization. Production of N fertilizers, however, consumes huge amounts of energy and the loss of excess N fertilizers to leaching results in the pollution of waterways and oceans. Therefore, increasing plant nitrogen use efficiency (NUE) is essential to help farmers produce more while conserving the environment. This book assembles some of the best work of top researchers from academic and industrial institutions in the area of NUE and provides valuable insight to scholars and researchers by its comprehensive discussion of current and future strategies to improve NUE through genetic manipulation. This book should also be highly valuable to policy makers, environmentalists, farmers, biotechnology executives, and to the hard-core researchers working in the lab.

This book describes the basic botanical features of kiwifruit and its wild relatives, reports on the steps that led to its genome sequencing, and discusses the results obtained with the assembly and annotation. The core chapters provide essential insights into the main gene families that characterize this species as a crop, including the genes controlling sugar and starch metabolism, pigment biosynthesis and degradation, the ascorbic-acid pathway, fruit softening and postharvest metabolism, allergens, and resistance to pests and diseases. The book offers a valuable reference guide for taxonomists, geneticists and horticulturists. Further, since information gained from the genome sequence is extraordinarily useful in assessing the breeding value of individuals based on whole-genome scans, it will especially benefit plant breeders. Accordingly, chapters are included that focus on gene introgression from wild relatives and genome-based breeding.

This book will shed light on the effect of salt stress on plants development, proteomics, genomics, genetic engineering, and plant adaptations, among other topics. Understanding the molecular basis will be helpful in developing selection strategies for improving salinity tolerance. The book will cover around 25 chapters with contributors from all over the world.

This volume aims to present a representative cross-section of modern experimental approaches relevant to Plant Hormone Biology, ranging from relatively simple physiological to highly sophisticated methods. Chapters describe physiological, developmental, microscopy-based techniques, measure hormone contents, and heterologous systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Hormones:Methods and Protocols, Third Edition aims to provide researchers with useful methods to advance their research.

Plant biotechnology has now become a key tool in improving crop productivity and enhancing commercial value of plant products. The book complies various methods of in vitro propagation and genetic manipulation of important aromatic and medicinal plants. It puts together latest techniques and innovations in the field of plant biotechnology such as effective protocols of genetic manipulation, isolation of secondary metabolites, use of somaclonal variation, stress management in plants. It also explores the role of various physiological and biochemical factors affecting the genetic stability of in-vitro cultured plants. These themes are of interest to both graduate and postgraduate students. Further this book will be useful for to researchers, academicians and industrialist to review latest progress and future prospects of these technologies.

Ascorbic acid (AsA), vitamin C, is one of the most abundant water-soluble antioxidant in plants and animals. In plants AsA serves as a major redox buffer and regulates various physiological processes controlling growth, development, and stress tolerance. Recent studies on AsA homeostasis have broadened our understanding of these physiological events. At the mechanistic level, AsA has been shown to participate in numerous metabolic and cell signaling processes, and the dynamic relationship between AsA and reactive oxygen species (ROS) has been well documented. Being a major component of the ascorbate-glutathione (AsA-GSH) cycle, AsA helps to modulate oxidative stress in plants by controlling ROS detoxification alone and in co-operation with glutathione. In contrast to the single pathway responsible for AsA biosynthesis in animals, plants utilize multiple pathways to synthesize AsA, perhaps reflecting the importance of this molecule to plant health. Any fluctuations, increases or decreases, in cellular AsA levels can have profound effects on plant growth and development, as AsA is associated with the regulation of the cell cycle, redox signaling, enzyme function and defense gene expression. Although there has been significant progress made investigating the multiple roles AsA plays in stress tolerance, many aspects of AsA-mediated physiological responses require additional research if AsA metabolism is to be manipulated to enhance stress-tolerance. This book summarizes the roles of AsA that are directly or indirectly involved in the metabolic processes and physiological functions of plants. Key topics include AsA biosynthesis and metabolism, compartmentation and transport, AsA-mediated ROS detoxification, as well as AsA signaling functions in plant growth, development and responses to environmental stresses. The main objective of this volume is therefore to supply comprehensive and up-to-date information for students, scholars and scientists interested in or currently engaged in AsA research.