This book caters to the need of researchers working in the ever-evolving field of agricultural biotechnology. It discusses and provides in-depth information about latest advancements happening in this field. The book discusses evolution of plant tissue culture techniques, development of doubled haploids technology, role of recombinant-DNA technology in crop improvement. It also provides an insight into the global status of genetically modified crops, use of RNAi technology and mi-RNAs in plant improvement. Chapters are also dedicated for different branches of 'omics' science including genomics, bioinformatics, proteomics, metabolomics and phenomics along with the use of molecular markers in tagging and mapping of various genes/QTLs of agronomic importance. This book also covers the role of enzymes and microbes in agriculture in productivity enhancement. It is of interest to teachers, researchers of biotechnology and agriculture scientists. Also the book serves as additional reading material for undergraduate and postgraduate students of biotechnology, agriculture, horticulture, forestry, ecology, soil science, and environmental sciences. National and international biotechnologists and agricultural scientists will also find this to be a useful read.

This book gathers the latest information on the organization of genomes in wild Solanum species and emphasizes how this information is yielding direct outcomes in the fields of molecular breeding, as well as a better understanding of both the patterns and processes of evolution. Cultivated Solanums, such as potato, tomato, and pepper, possess a high number of wild relatives that are of great importance for practical breeding and evolutionary studies. Their germplasm is often characterized by allelic diversity, as well as genes that are lacking in the cultivated species. Wild Solanums have not been fully exploited by breeders. This is mainly due to the lack of information regarding their genetics and genomics. However, the genome of important cultivated Solanaceae such as potato, tomato, eggplant, and pepper has already been sequenced. On the heels of these recent developments, wild Solanum genomes are now becoming available, opening an exciting new era for both basic research and varietal development in the Solanaceae.

During the past 15 years, cellular and molecular approaches have emerged as valuable adjuncts to supplement and complement conventional breeding methods for a wide variety of crop plants. Biotechnology increasingly plays a role in the creation, conservation, characterization and utilization of genetic variability for germplasm enhancement. For instance, anther/microspore culture, somaclonal variation, embryo culture and somatic hybridization are being exploited for obtaining incremental improvement in the existing cultivars. In addition, genes that confer insect- and disease-resistance, abiotic stress tolerance, herbicide tolerance and quality traits have been isolated and re-introduced into otherwise sensitive or susceptible species by a variety of transgenic techniques. Together these transformative methodologies grant access to a greater repertoire of genetic diversity as the gene(s) may come from viruses, bacteria, fungi, insects, animals, human beings, unrelated plants or even be artificially derived. Remarkable achievements have been made in the production, characterization, field evaluation and commercialization of transgenic crop varieties worldwide. Likewise, significant advances have been made towards increasing crop yields, improving nutritional quality, enabling crops to be raised under adverse conditions and developing resistance to pests and diseases for sustaining global food and nutritional security. The overarching purpose of this 3-volume work is to summarize the history of crop improvement from a technological perspective but to do so with a forward outlook on further advancement and adaptability to a changing world. Our carefully chosen "case studies of important plant crops" intend to serve a diverse spectrum of audience looking for the right tools to tackle complicated local and global issues.

This book provides essential insights into methods and practices of 'Climate-smart Agriculture,' which is driven by the principles of climate resilience and smart resource use in agricultural production. Climate-smart agriculture is a key policy instrument for achieving poverty eradication and a hunger-free world, as well as mitigating the effects of climate change. This book discusses in detail climate-smart agricultural technologies and practices that can reduce the vulnerability of agricultural systems, improve the livelihoods of farmers and other stakeholders, and reduce the greenhouse gas emissions from crop production and livestock husbandry. The agriculture, forestry and other land use (AFOLU) sector produces roughly 10-12 gigatons of CO2-equivalent per year; therefore, sustainable practices for agriculture and related land use hold immense potential to mitigate climate change. The potential impacts of climate variability and climate change on agriculture are extensively documented and articulated, especially with regard to global and national environmental agendas that call for innovation, transformation and climate-resilient advances in agriculture. As the book demonstrates, climate-smart agriculture offers an excellent tool for boosting agricultural output to feed the growing global population; for reducing greenhouse gases emissions from agriculture and other land use; and for protecting agricultural production systems from the impending dangers of climate change.

Plants require essential nutrients (macronutrients and micronutrients) for normal functioning. Sufficiency range is the levels of nutrients necessary to meet the plant's needs for optimal growth. This range depends on individual plant species and the particular nutrient. Nutrient levels outside of a plant's sufficiency range cause overall crop growth and health to decline, due either to deficiency or toxicity from over-accumulation. Apart from micronutrients (B, Cl, Mn, Fe, Zn, Cu and Mo), Aluminum (Al), cerium (Ce), cobalt (Co), iodine (I), lanthanum (La), sodium (Na), selenium (Se), silicon (Si), titanium (Ti), and vanadium (V) are emerging as novel biostimulants that may enhance crop productivity and nutritional quality. These beneficial elements are not "essential" but when supplied at low dosages, they augment plant growth, development, and yield by stimulating specific molecular, biochemical, and physiological pathways in responses to challenging environments. The book is the first reference volume that approaches plant micronutrient management with the latest biotechnological and omics tools. Expertly curated chapters highlight working solutions as well as open problems and future challenges in plant micronutrient deficiency or toxicity. We believe this book will introduce readers to state-of-the-art developments and research trends in this field.

This book focusing on the bitter gourd genome is the first comprehensive compilation of knowledge on the botany, cytogenetical analysis, genetic resources and diversity, traditional breeding, tissue culture and genetic transformation, whole genome sequencing and comparative genomics in the Cucurbitaceae family. It discusses the biochemical profile of the bioactives present in this horticultural crop, used both as a vegetable and as a medicine, and also addresses sex determination in bitter gourd. Written by respected international experts, the book is useful to students, teachers and scientists in academia, as well as seed companies and pharmaceutical industries.

Plant tissue culture (PTC) technology has gained unassailable success for its various commercial and research applications in plant sciences. Plant growth regulators (PGRs) are an essential part of any plant tissue culture intervention for propagation or modification of plants. A wide range of PGRs are available, including aromatic compounds that show cytokinin activities, promote cell division and micro-propagation, viz. kinetin, N6-benzyladenine and topolins. Topolins are naturally occurring aromatic compounds that have gained popularity as an effective alternative for other frequently used cytokinins in in vitro culture of plants. Among them, meta-topolin [6-(3-hydroxybenzlyamino) purine] is the most popular and its use in plant tissue culture has amplified swiftly. During the last few decades, there have been numerous reports highlighting the effectiveness of meta-topolin in micropropagation and alleviation of various physiological disorders, rooting and acclimatization of tissue culture raised plants.

Sorghum is the most important cereal crop grown in the semi-arid tropics (SAT) of Africa, Asia, Australia and Americas for food, feed, fodder and fuel. It is the fifth most important cereal crop globally after rice, wheat, maize and barley, and plays a major role in global food security. Sorghum is consumed in different forms for various end-uses. Its grain is mostly used directly for food purposes. After the release of the proceedings of two international symposia in the form of books "Sorghum in Seventies" and "Sorghum in Eighties", global sorghum research and development have not been documented at one place. Of course, few books on sorghum have been released that focus on specific issues/research areas, but comprehensive review of all aspects of recent development in different areas of sorghum science has not been compiled in the form a single book. This book is intended to fill in a void to bridge the gap by documenting all aspects of recent research and development in sorghum encompassing all the progress made, milestones achieved across globe in genetic diversity assessment, crop improvement and production, strategies for high yield, biotic and abiotic stress resistance, grain and stover quality aspects, storage, nutrition, health and industrial applications, biotechnological applications to increase production, including regional and global policy perspectives and developmental needs. This book will be an institutional effort to compile all the latest information generated in research and development in sorghum across the globe at one place.

This comprehensive guide to potato production systems management contains 20 chapters and more than 350 color photographs. Beginning with the history of potato culture, it spans all aspects of potato production, pest and planting management, storage, and marketing. Written by a team of over 35 scientists from North America, this book offers updated research-based information and serves as a unique, valuable tool for researchers, extension specialists, students, and farm managers. More than a description of principles, it contains practical analytical tools, charts, and methods to create guidelines for best production practices and cost estimates. Some key areas include: Potato Growth and Development, Potato Variety Selection and Management, Seed and Planting Management, Seed Production and Certification, Field Selection, Crop Rotation, and Soil Management, Integrated Pest Management for Potatoes, Potato Nutrient Management, Irrigation Management, Tuber Quality, Economics and Marketing, Production Costs, among others. Potato Production Systems should be a valuable reference for successful culture of the "noble tuber."

Linum (flax) is a genus of about 200 species in the flowering plant family Linaceae. The genus includes common flax, which is one of the best fibers to produce linen, the seeds to produce linseed oil and has health-related properties of flax in human and animal nutrition. This book describes the genetics and genomics of Linum including the development of extensive experimental resources (e.g. whole genome sequence, efficient transformation methods, insertional mutant collections, large germplasm collections, resequenced genomes) that have led much progress and its economic importance. The methods and use of Linum to address a wide range of applications (e.g. disease resistance, cell wall composition, abiotic stress tolerance, floral development, natural diversity) is also discussed.

This book discusses the latest developments in plant-mediated fabrication of metal and metal-oxide nanoparticles, and their characterization by using a variety of modern techniques. It explores in detail the application of nanoparticles in drug delivery, cancer treatment, catalysis, and as antimicrobial agent, antioxidant and the promoter of plant production and protection. Application of these nanoparticles in plant systems has started only recently and information is still scanty about their possible effects on plant growth and development. Accumulation and translocation of nanoparticles in plants, and the consequent growth response and stress modulation are not well understood. Plants exposed to these particles exhibit both positive and negative effects, depending on the concentration, size, and shape of the nanoparticles. The impact on plant growth and yield is often positive at lower concentrations and negative at higher ones. Exposure to some nanoparticles may improve the free-radical scavenging potential and antioxidant enzymatic activities in plants and alter the micro-RNAs expression that regulate the different morphological, physiological and metabolic processes in plant system, leading to improved plant growth and yields. The nanoparticles also carry out genetic reforms by efficient transfer of DNA or complete plastid genome into the respective plant genome due to their miniscule size and improved site-specific penetration. Moreover, controlled application of nanomaterials in the form of nanofertilizer offers a more synchronized nutrient fluidity with the uptake by the plant exposed, ensuring an increased nutrient availability. This book addresses these issues and many more. It covers fabrication of different/specific nanomaterials and their wide-range application in agriculture sector, encompassing the controlled release of nutrients, nutrient-use efficiency, genetic exchange, production of secondary metabolites, defense mechanisms, and the growth and productivity of plants exposed to different manufactured nanomaterials. The role of nanofertilizers and nano-biosensors for improving plant production and protection and the possible toxicities caused by certain nanomaterials, the aspects that are little explored by now, have also been generously elucidated.

Translational medicine addresses the gap between research and the clinical application of new discoveries. To efficiently deliver new drugs to care centers, a preclinical evaluation, both in vitro and in vivo, is required to ensure that the most active and least toxic compounds are selected as well as to predict clinical outcome. Antimicrobial nanomedicines have been shown to have higher specificity in their therapeutic targets and the ability to serve as adjuvants, increasing the effectiveness of pre-existing immune compounds. The design and development of new standardized protocols for evaluating antimicrobial nanomedicines is needed for both the industry and clinical laboratory. These protocols must aim to evaluate laboratory activity and present models of pharmacokinetic-pharmacodynamic and toxicokinetic behavior that predict absorption and distribution. Likewise, these protocols must follow a theranostics approach, be able to detect promising formulations, diagnose the infectious disease, and determine the correct treatment to implement a personalized therapeutic behavior. Given the possibilities that nanotechnology offers, not updating to new screening platforms is inadequate as it prevents the correct application of discoveries, increasing the effect of the valley of death between innovations and their use. This book is structured to discuss the fundamentals taken into account for the design of robust, reproducible and automatable evaluation platforms. These vital platforms should enable the discovery of new medicines with which to face antimicrobial resistance (RAM), one of the great problems of our time.

This book includes papers presented at the 2017 Joint meeting of Fodder Crops and Amenity Grasses Section and Protein Crops Working Group of EUCARPIA-Oil and Protein Crops Section. The theme of the meeting "Breeding Grasses and Protein Crops in the Era of Genomics" has been divided into six parts: (1) Utilisation of genetic resources and pre-breeding, (2) Genetic improvement of quality and agronomic traits, (3) Breeding for enhanced stress tolerance (4) Implementation of phenomics and biometrics, (5) Development of genomic tools and bioinformatics and (6) Reports of Parallel Sessions.

This book provides an indispensable reference guide to the sustainable control and treatment of biomass residues from a wide variety of agroindustrial sources, e.g. sugarcane, livestock, pulp & paper, food wastes, among others. Pursuing a structured and clear approach, the book opens with a general introduction to biomass, sustainability and environmental chemistry aspects, and on how the use of biomass as a renewable material ties into the UN's Sustainable Development Goals. The book subsequently presents analytical methods applied to different biomass types and their residues and reviews monitoring and treatment strategies in order to avoid pollution of the same. The book closes by describing the value chains, bioeconomy and circular economy for globally relevant agroindustrial biomass. The book is intended for researchers in academia and industry alike and shows how, in addition to sustainability criteria and life cycle assessments, integrating environmental chemistry aspects can contribute to a holistic approach, and unlock the economic potential of biomass in the age of circular economy and sustainable development.

This book provides in-depth reviews of the role of Rhizobium in agriculture and its biotechnological applications. Individual chapters explore topics such as: the occurrence and distribution of Rhizobium; phenotypic and molecular characteristics of Rhizobium; impact of Rhizobium on other microbial communities in the rhizosphere; N2-fixation ability of Rhizobium; Rhizobium and biotic stress; Rhizobium-mediated restoration of an ecosystem; in silico analysis of the rhizobia pool; further biotechnological perspectives of Rhizobium.

This book comprehensively introduces all aspects of the physiology, stress responses and tolerance to abiotic stresses of the Fabaceae plants. Different plant families have been providing food, fodder, fuel, medicine and other basic needs for the human and animal since the ancient time. Among the plant families Fabaceae have special importance for their agri-horticultural importance and multifarious uses apart from the basic needs. Interest in the response of Fabaceae plants toward abiotic stresses is growing considering the economic importance and the special adaptive mechanisms. Recent advances and developments in molecular and biotechnological tools has contributed to ease and wider this mission. This book provides up-to-date findings that will be of greater use for the students and researchers, particularly Plant Physiologists, Environmental Scientists, Biotechnologists, Botanists, Food Scientists and Agronomists, to get the information on the recent advances on this plant family in regard to physiology and stress tolerance.

Nature, by dint of its constitution, harbors many unassuming mysteries broadly manifested by its constituent cohorts. If physics is the pivot that holds nature and chemistry provides reasons for its existence, then the rest is just manifestation. Nanoscience and technology harbor the congruence of these two core subjects, whereby many phenomenon may be studied in the same perspective. That nature operates at nanoscale-obeying the principles of thermodynamics and supramolecular chemistry-is a well understood fact manifested in a variety of life processes: bones are restored after a fracture; clots potentially leading to cerebral strokes can be dissolved. The regeneration of new structures in our system follows a bottom-up approach. Be it a microbe (benign or pathogenic), plant (lower or higher), plant parts/organs, food beneficiaries, animal (lower), higher animal processing wastes, these all are found to deliver nanomaterials under amenable processing conditions. Identically, the molecules also seem to obey the thermodynamic principles once they get dissociated/ionized and the energy captured in the form of bonding helps in the synthesis of a myriad of nanomaterials. This edited volume explores the various green sources of nanomaterial synthesis and evaluates their industrial and biomedical applications with a scope of scaling up. It provides useful information to researchers involved in the green synthesis of nanomaterials in fields ranging from medicine to integrated agricultural management.

This book provides insights into the current state of sorghum genomics. It particularly focuses on the tools and strategies employed in genome sequencing and analysis, public and private genomic resources and how all this information is leading to direct outcomes for plant breeders. The advent of affordable whole genome sequencing in combination with existing cereal functional genomics data has enabled the leveraging of the significant novel diversity available in sorghum, the genome of which was fully sequenced in 2009, providing an unmatched resource for the genetic improvement of sorghum and other grass species. Cultivated grain sorghum is a food and feed cereal crop adapted to hot and dry climates, and is a staple for 500 million of the world's poorest people. Globally, sorghum is also an important source of animal feed and forage, an emerging biofuel crop and model for C4 grasses, particularly genetically complex sugarcane.

With the recent shift of chemical fertilizers and pesticides to organic agriculture, the employment of microbes that perform significant beneficial functions for plants has been highlighted. This book presents timely discussion and coverage on the use of microbial formulations, which range from powdered or charcoal-based to solution and secondary metabolite-based bioformulations. Bioformulation development of biofertilizers and biopesticides coupled with the advantages of nanobiotechnology propose significant applications in the agricultural section including nanobiosensors, nanoherbicides, and smart transport systems for the regulated release of agrochemical. Moreover, the formulation of secondary metabolites against individual phytopathogens could be used irrespective of geographical positions with higher disease incidences. The prospective advantages and uses of nanobiotechnology generate tremendous interest, as it could augment production of agricultural produce while being cost-effective both energetically and economically. This bioformulation approach is incomparable to existing technology, as the bioformulation would explicitly target the particular pathogen without harming the natural microbiome of the ecosystem. Nanobiotechnology in Bioformulations covers the constraints associated with large-scale development and commercialization of bioinoculant formations. Furthermore, exclusive emphasis is be placed on next-generation efficient bioinoculants having secondary metabolite formulations with longer shelf life and advanced competence against several phytopathogens. Valuable chapters deal with bioformulation strategies that use divergent groups of the microbiome and include detailed diagrammatic and pictorial representation. This book will be highly beneficial for both experts and novices in the fields of microbial bioformulation, nanotechnology, and nano-microbiotechnology. It discusses the prevailing status and applications available for microbial researchers and scientists, agronomists, students, environmentalists, agriculturists, and agribusiness professionals, as well as to anyone devoted to sustaining the ecosystem.

This book includes papers from keynote lecture and oral presentations of Plant and Microbe Adaptations to Cold (PMAC) 2012, an international conference on winter hardiness of crop and pathogenic microbes. The PMAC has been started in 1997 in Japan as an interdisciplinary forum for scientists and extension people working in the field in plant pathology, plant physiology, microbiology, and crop breeding to increase our knowledge and improve our understanding of overwintering of crops, forages and grasses and solve the problems associated with losses due to freezing and heavy snow cover. Successive meetings have been held in Iceland (2000), Canada (2003), Italy (2006), and Norway (2009). PMAC2012 will be a special meeting with a focus on global climate change, food security and agriculture sustainability and the whole program will be arranged to reflect this theme. The topics covered by this proceedings includes, global warming in agricultural environment, plant adaptations to cold, microbial adaptations to cold, plant-microbe interaction under cold, and molecular breeding for winter hardiness. The researches range from molecular biology to ecology and breeding. Experts in the field will report cutting edge research and thoughtful strategies for sustainability.

This volume showcases current ethnobiological accounts of the ways that people use plants to promote human health and well-being. The goal in this volume is to highlight some contemporary examples of how plants are central to various aspects of healthy environments and healthy minds and bodies. Authors employ diverse analytic frameworks, including: interpretive and constructivist, cognitive, political-ecological, systems theory, phenomenological, and critical studies of the relationship between humans, plants and the environment. The case studies represent a wide geographical range and explore the diversity in the health appeals of plants and herbs. The volume begins by considering how plants may intrinsically be 'healthful' and the notion that ecosystem health may be a literal concept used in contemporary efforts to increase awareness of environmental degradation. The book continues with the exploration of the ways in which medically-pluralistic societies demonstrate the entanglements between the environment, the state and its citizens. Profit driven models for the extraction and production of medicinal plant products are explored in terms of health equity and sovereignty. Some of the chapters in this volume work to explore medicinal plant knowledge and the globalization of medicinal plant knowledge. The translocal and global networks of medicinal plant knowledge are pivotal to productions of medicinal and herbal plant remedies that are used by people in all variety of societies and cultural groups. Humans produce health through various means and interact with our environments, especially plants, in order to promote health. The ethnographic accounts of people, plants, and health in this volume will be of interest to the fields of anthropology, biology and ethnobiology, as well as allied disciplines.

Dothistroma pini changed New Zealand commercial forestry dramatically. Tree breeding became concentrated on a very few species and development of selection methods and breeding strategies changed in response to the new challenges. Tree-Breeding and Genetics in New Zealand provides a critical historical account of the work on provenance research and tree breeding, often with the wisdom of hindsight, and it tracks the development of breeding strategy, especially for P. radiata, Douglas-fir and the most important eucalypt species, E. regnans, E. fastigata and E. nitens. The book is a compendium of abstracts and summaries of all publications and reports on tree improvement in New Zealand since the early 1950s, with added critical comment by the author on much of the work. It is intended for other tree breeders internationally, for interested NZ foresters and for graduate students studying genetics and tree breeding.

This comprehensive volume covers recent studies into agricultural problems caused by soil and water contamination. Considering the importance of agricultural crops to human health, the editors have focused on chapters detailing the negative impact of heavy metals, excessive chemical fertilizer use, nutrients, pesticides, herbicides, insecticides, agricultural wastes and toxic pollutants, among others, on agricultural soil and crops. In addition, the chapters offer solutions to these negative impacts through various scientific approaches, including using biotechnology, nanotechnology, nutrient management strategies, biofertilizers, as well as potent PGRs and elicitors. This book serves as a key source of information on scientific and engineered approaches and challenges for the bioremediation of agricultural contamination worldwide. This book should be helpful for research students, teachers, agriculturalists, agronomists, botanists, and plant growers, as well as in the fields of agriculture, agronomy, plant science, plant biology, and biotechnology, among others. It serves as an excellent reference on the current research and future directions of contaminants in agriculture from laboratory research to field application.

The orchid family is one of the largest families of flowering plants known for their beauty and economic importance. This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing.This book is divided into 5 parts. The first part emphasizes propagation methods using seeds and related techniques that are important to plant conservation and improvement. Successes in asymbiotic and symbiotic seed germination are keys to orchid conservation and their propagation. The second part summarizes micropropagation methods, common media, and newer methods of micropropagation such as the bioreactor culture procedures. The third part focuses on techniques related to the manipulation of explants in an in vitro environment. The fourth part covers cell biological methods and transformation techniques. Since the successes in a laboratory setting do not guarantee plant survival and propagation in greenhouses and in the natural environment, it discusses greenhouse propagation techniques that are essential to the survival of plants generated from a laboratory setting. The fifth part showcases recent successes on orchid propagation by documenting sample publications and how to present orchids in an artistic fashion for one's enjoyment.

This book is devoted to grain legumes and include eight chapters devoted to the breeding of specific grain legume crops and five general chapters dealing with important topics which are common to most of the species in focus. Soybean is not included in the book as it is commonly considered an oil crop more than a grain legume and is included in the Oil Crops Volume of the Handbook of Plant Breeding. Legume species belong to the Fabaceae family and are characterized by their fruit, usually called pod. Several species of this family were domesticated by humans, such as soybean, common bean, faba bean, pea, chickpea, lentil, peanut, or cowpea. Some of these species are of great relevance as human and animal food. Food legumes are consumed either by their immature pod or their dry seeds, which have a high protein content. Globally, grain legumes are the most relevant source of plant protein, especially in many countries of Africa and Latin America, but there are some constraints in their production, such as a poor adaptation, pest and diseases and unstable yield. Current research trends in Legumes are focused on new methodologies involving genetic and omic studies, as well as new approaches to the genetic improvement of these species, including the relationships with their symbiotic rhizobia.