• Step-by-step reproducible laboratory procedures written by the top experts in the field offering their time-tested hints, tricks, and tips.
• Presents detailed outlines of embryonic systems models along with cutting-edge cellular, genetic, and molecular mechanism.
• Provides the most comprehensive collection of protocols on plan embryo development.

This book presents selected papers from the 8th International Conference on Information and Communication Technologies in Agriculture, Food and Environment (HAICTA 2017) which examine sustainable rural development in the context of environmental, economic, and the socio-cultural dimension. This book raises awareness of the importance of sustainable management in agriculture using examples of actual industry cases, sustainable management practices, new forms of rural cooperation and entrepreneurship.

This book contains papers and posters presented at the 18th Eucarpia Fodder Crops Section Meeting held at Loen, Nordfjord, Norway in August 1993. In most environments some form of marginal conditions or stress prevails. Few crops are being produced under such a wide range of environmental and management stresses as fodder crops. Improved adaptation of fodder crops to marginal conditions is crucial in developing sustainable, low-input agricultural systems. The book is unique in demonstrating the large diversity both in crops and environmental stresses that confront the forage breeders. Both general and specific aspects of adaptation to marginal growing conditions are presented, ranging from problems caused by snow and ice in the Subarctic regions of Europe to the severe drought problems in the Mediterranean regions. For everyone involved in studies of adaptation and breeding of perennial plants for marginal conditions or stress environments.

This book reviews the latest advances in multiple fields of plant biotechnology and the opportunities that plant genetics, genomics and molecular biology have offered for agriculture improvement. Advanced technologies can dramatically enhance our capacity in understanding the molecular basis of traits and utilizing the available resources for accelerated development of high yielding, nutritious, input-use efficient and climate-smart crop varieties. In this book, readers will discover the significant advances in plant genetics, structural and functional genomics, trait and gene discovery, transcriptomics, proteomics, metabolomics, epigenomics, nanotechnology and analytical & decision support tools in breeding. This book appeals to researchers, academics and other stakeholders of global agriculture.

In continuation of Volumes 8, 9, 22, and 23, this new volume deals with the regeneration of plants from isolated protoplasts and genetic transformation in various species of "Actinidia," "Allocasuarina," "Anthurium," "Antirrhinum," "Asparagus," "Beta," "Brassica," "Carica," "Casuarina," "Cyphomandra," "Eucalyptus," "Ipomoea," "Larix," "Limonium," "Liriodendron," "Malus," "Musa," "Physcomitrella," "Physalis," "Picea," "Rosa," "Tagetes," "Triticum," and "Ulmus."
These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. The book contains a wealth of useful information for advanced students, teachers, and researchers in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general biotechnology.

The inflorescence of the monoecious maize plant is unique among the Gramineae in the sharp separation of the male and female structures. The male tassel at the terminus of the plant most often sheds pollen before the visual appearance of the receptive silks of th the female ear at a lateral bud, normally at the 10 leaf [I]. Earlier studies examined the ontogeny of the growing tissues beginning with the embryo in the kernel through to the obvious protuberances of the growing point as the kernel germinates. The differentiated developing soon-to-become tassel and the lateral bulges that develop into the ears on the lateral buds become apparent very early in the germinating kernel [2, 3, 46]. A certain number of cells are destined for tassel and ear development [8]. As the plant develops, there is a phase transition [\3, 16] from the vegetative lateral buds to the reproductive lateral buds. This change in phase has been ascribed to genotypic control as evidenced in the differences among different genotypes in the initiation of the reproductive [I]. The genetic control of tassel and ear initiation has been gleaned from anatomical observations. Lejeune and Bernier [I2] found that maize plants terminate the initiation of additional axillary meristems at the time of tassel initiation. This would indicate that the top-most ear shoot is initiated on the same day as the initiation of tassel development and this event signals the end of the undifferentiated growing point.

In the past there were many attempts to change natural foodstuffs into high-value products. Cheese, bread, wine, and beer were pro duced, traditionally using microorganisms as biological tools. Later, people influenced the natural process of evolution by artificial selection. In the 19th century, observations regarding the depen dence of growth and reproduction on the nutrient supply led to the establishment of agricultural chemistry. Simultaneously, efforts were directed at defining the correlation between special forms of morphological differentiation and related biochemical processes. New experimental systems were developed after the discovery of phytohormones and their possible use as regulators of growth and differentiation. In these systems, intact plants or only parts of them are cultivated under axenic conditions. These methods, called "in vitro techniques," were introduced to modern plant breeding. In the field of basic research, plant cell cultures were increasingly developed and the correlations between biochemical processes and visible cell variations were explored further. It should be possible to manipulate the basic laws of regulation and the respective biochemi cal processes should be regarded as being independent of morpho logical processes of plant development."

This book volume has been divided into three sections and contains a total of 23 chapters. Section A contains eleven chapters covering topics such as studies of embryo development and cell biology of white spruce, proliferative somatic embryogenesis in woody species, somatic embryo germination and desiccation tolerance in conifers, performance of conifer somatic seedlings, apoptosis during early somatic embryogenesis, water relation parameters in conifer embryos, image analysis of somatic embryos, somatic embryogenesis in woody legumes, cold storage and crypreservation, and commercialization of plant somatic embryogenesis. Section B comprisis six chapters dealing with angiosperm woody plants such as somatic embryogenesis in myrtaceous plants, Laurus nobilis, Simarouba glauca, Magnolia spp., Juglans cinera, and somatic embryogenesis and evaluation of variability in somatic seedlings of Quercus serrata by RAPD markers. The chapters contained in Section C are focussed on somatic embryogenesis in gymnosperms, including Pinus patula, Encephalartos, Picea wilsonii, Pinus banksiana, hybrid firs, and Taxus. All the mansucripts have been peer reviewed and revised accordingly to improve the quality of these chapters. The final manuscripts were submitted as camera- ready to publication, and editors had no opportunity to go through them again before the final printing. Authors were advised to prepare final camera-ready manuscripts carefully to avoid any mistakes. Therefore, editors are not respon- sible for mistakes, if any, in this book volume. We are grateful to all the book chapter contributors for submitting their manuscripts in time, and to the reviewers for giving their free time to review the manuscripts.

Genetically Modified Plants, Second Edition, provides an updated roadmap and science-based methodology for assessing the safety of genetic modification technologies, as well as risk assessment approaches from regulators across different agroecosystems. This new edition also includes expanded coverage of technologies used in plant improvement, such as RNA-dependent DNA methylation, reverse breeding, agroinfiltration, and gene-editing technologies such as CRISPR and TALENS. This book is an essential resource for anyone interested in crop improvement, including students and researchers, practitioners in regulatory agencies, and policymakers involved in plant biotechnology risk assessment.

In order to feed the world, global agriculture will have to double food production by 2050. As a result, the use of soils with fertilizers and pesticides in agronomic ecosystems will increase, taking into account the sustainability of these systems and also the provision of food security. Thus, soil ecosystems, their health, and their quality are directly involved in sustainable agronomical practices, and it is important to recognize the important role of soil microbial communities such as mycorrhizal fungi, their biodiversity, interactions, and functioning. Soil ecosystems are under the threat of biodiversity loss due to an increase of cultivated areas and agronomic exploitation intensity. Also, changes in land use alter the structure and function of ecosystems where biodiversity is vital in the ecosystem. Soils are a major aid in food production in all terrestrial ecosystems; however, this means they are also involved in gas emission and global warming. Thus, in agronomic ecosystems, several mitigation practices have been proposed to promote the increase of carbon soil stock, and the reduction of warming gas emission from soils. In South America, most of the rural population depends economically on agriculture and usually works in family units. New, organic, safe, and sustainable agro-forestry practices must be applied to support local communities and countries to achieve hunger eradication, rural poverty reduction, and sustainable development. This book compiles new information for mycorrhizal occurrence in natural and anthropic environments in South America. It includes new reports of mycorrhizal fungi diversity along different mycorrhizal types and their effect on plant communities, plant invasions, the use of mycorrhizal fungi for ecological and sustainable studies, management programs of natural and agroecosystems, and forestry and food-secure production. This book fills the gaps in biodiversity knowledge, management and safe food production of mycorrhizas. It should be a valuable help to researchers, professors and students, to aid in use of mycorrhizal fungi while also focusing on their biodiversity, sustainable safe food production, and conservation perspectives.

Fantasies and dreams have their rightful place in science, and sometimes they turn into reality. Regeneration of hybrid plants through protoplast fusion is one such dream come true. In the early 1970s I shared the pioneering excitement in the field of protoplast technology at the Second International Congress of Plant Tissue Culture held in Strasbourg, France. Subsequently, I participated in three international conferences devoted to plant protoplasts, in Salamanca, Spain (1972), Versailles, France (1972), and Nottingham, England (1975). At Versailles Dr. P.S. Carlson presented his work on the successful regeneration of somatic hybrids between Nicotiana glauca and Nicotiana langsdorfii. The enthusi- asm shown by the participants was sufficient indication of the bright future of somatic hybridization. On my return from Versailles, I gathered my thoughts and prepared a concept paper on Potentials of Protoplast Culture Work in Agriculture which was published in Euphytica (Bajaj 1974). The studies on protoplast fusion and somatic hybridization then gained momentum and active work started in many laboratories. Very significant work was done by Melchers et al. (1978) who obtained a somatic hybrid between potato and tomato, calling it "Pomato".

This book collates various aspects of stress tolerance in crop plants. It primarily focuses on the heat and temperature related stress, starting from the severity of the problem on quantity and quality of yield under the threat of global climate change. The content also explores other mechanistic dimensions such as physiochemical and molecular mechanism underlying thermotolerance, signaling mechanism under heat stress, role of heat shock proteins in modulating thermotolerance, omics approach for development of climate smart-crop. Chapters discuss different approaches used in the past to develop heat stress tolerant crop plants, list of developed thermotolerant agriculturally important crop plants, redox homeostasis under heat stress, nutrient uptake and use efficiency in plants under heat stress and much more. The book is a useful compilation for researchers working in the area of abiotic stress tolerance in crop plants, as well as for students of plant physiology and agricultural sciences.

Microbial biosurfactant compounds are a group of structurally diverse molecules produced by microorganisms, and are mainly categorized according to their chemical structure. The diversity of microbial biosurfactants makes them versatile and means that they offer a range of capabilities, while at the same time being economically sustainable. As such, they have potential applications in environmental processes, as well as in food, biomedicine and other industries. This book discusses innovative approaches and cutting-edge research that utilize the various properties of biosurfactants. Drawing on research from around the globe, it provides an up-to-date review of biosurfactant applications and their importance in fields such as medicine, gene therapy, immunotherapy, antimicrobial bioremediation and agriculture. It also discusses their anti-adhesive properties. The book will appeal to academics and researchers in the field of microbiology, as well as policymakers. It also serves as additional reading material for undergraduate and graduate students of agriculture, ecology, soil science, and environmental sciences.

Automation and Environmental Control in Plant Tissue Culture rigorously explores the new challenges faced by modern plant tissue culture researchers and producers worldwide: issues of cost efficiency, automation, control, and optimization of the in vitro microenvironment. This book achieves a critical balance between the economic, engineering and biological viewpoints, and presents well-balanced, unique, and clearly organized perspectives on current initiatives in the tissue culture arena. Each chapter offers guidelines leading towards an exhaustive, unprecedented level of control over in vitro growth, based on emerging technologies of robotics, machine vision, environmental sensors and regulation, and systems analysis. Unlike other tissue culture books which focus on specific crops and techniques, this book spans the broad range of major tissue culture production systems, and advances evidence on how some underrated aspects of the process actually determine the status of the end product. Key researchers from industry and academia have joined to give up-to-date research evidence and analysis. The collection comprises an essential reference for industrial-scale tissue culture producers, as well as any researcher interested in optimizing in vitro production.

Marker-assisted plant breeding involves the application of molecular marker techniques and statistical and bioinformatics tools to achieve plant breeding objectives in a cost-effective and time-efficient manner. This book is intended for beginners in the field who have little or no prior exposure to molecular markers and their applications, but who do have a basic knowledge of genetics and plant breeding, and some exposure to molecular biology. An attempt has been made to provide sufficient basic information in an easy-to-follow format, and also to discuss current issues and developments so as to offer comprehensive coverage of the subject matter. The book will also be useful for breeders and research workers, as it offers a broad range of up-to-the-year information, including aspects like the development of different molecular markers and their various applications. In the first chapter, the field of marker-assisted plant breeding is introduced and placed in the proper perspective in relation to plant breeding. The next three chapters describe the various molecular marker systems, while mapping populations and mapping procedures including high-throughput genotyping are discussed in the subsequent five chapters. Four chapters are devoted to various applications of markers, e.g. marker-assisted selection, genomic selection, diversity analysis, finger printing and positional cloning. In closing, the last two chapters provide information on relevant bioinformatics tools and the rapidly evolving field of phenomics.

The biomass based energy sector, especially the one based on lignocellulosic sources such as switchgrass Miscanthus, forest residues and short rotation coppice, will play an important role in our drive towards renewable energy. The biomass feedstock production (BFP) subsystem provides the necessary material inputs to the conversion processes for energy production. This subsystem includes the agronomic production of energy crops and the physical handling and delivery of biomass, as well as other enabling logistics. Achieving a sustainable BFP system is therefore paramount for the success of the emerging bioenergy sector. However, low bulk and energy densities, seasonal and weather sensitive availability, distributed supply and lack of commercial scale production experience create unique challenges. Moreover, novel region specific feedstock alternatives continue to emerge. Engineering will play a critical role in addressing these challenges and ensuring the techno-economic feasibility of this sector. It must also integrate with the biological, physical and chemical sciences and incorporate externalities, such as social/economic considerations, environmental impact and policy/regulatory issues, to achieve a truly sustainable system. Tremendous progress has been made in the past few years while new challenges have simultaneously emerged that need further investigation. It is therefore prudent at this time to review the current status and capture the future challenges through a comprehensive book. This work will serve as an authoritative treatise on the topic that can help researchers, educators and students interested in the field of biomass feedstock production, with particular interest in the engineering aspects.

The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put tremendous pressure on trees and tree products; growing awareness of the potential of previously un exploited tree resources and environmental pollution have both accelerated development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which cannot be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding may be a novel approach to improving and multiplying in large number the trees and woody plants. So far, plant tissue culture technology has largely been exploited in the propagation of ornamental plants, especially foliage house plants, by com- mercial companies. Generally, tissue culture of woody plants has been recal- citrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taeda), Sandalwood (Santalum album), Citrus, Mango (Mangifera indica), etc. offer a ray of hope of: (a) inexpensive clonal propa- gation for large-scale production of plants or "emblings" or "somatic embryo plants"; (b) protoplast work; (c) cryopreservation; (d) genetic transforma- tion; and (e) artificial or manufactured seed production.

This book provides a comprehensive description of phosphate solubilizing microorganisms and highlights methods for the use of microphos in different crop production systems. The focus is on understanding both the basic and applied aspects of phosphate solubilizing microorganisms and how phosphorus-deficient soils can be transformed into phosphorus-rich ones by applying phosphate solubilizing microorganisms. The interaction of rhizosphere phosphate solubilizing microorganisms and environmental variables, as well as their importance in the production of crops such as legumes, cereals, vegetables etc. are discussed and considered. The use of cold-tolerant phosphate solubilizing microorganisms to enhance crop productivity in mountainous regions is examined, as are the ecological diversity and biotechnological implications of phosphate solubilizing microorganisms. Lastly, the role of phosphate solubilizing microorganisms in aerobic rice cultivation is highlighted. This volume offers a broad overview of plant disease management using phosphate solubilizing microbes and presents strategies for the management of cultivated crops. It will therefore be of special interest to both academics and professionals working in the fields of microbiology, soil microbiology, biotechnology and agronomy, as well as the plant protection sciences. This timely reference book provides an essential and comprehensive source of material, as it includes recent findings on phosphate solubilizing microorganisms and their role in crop production.

Development of superior crops that have consistent performance in quality and in quantity has not received the same emphasis in the field of genetics and breeding as merited. Specialty trait requires special focus to propagate. Yet basic germplasm and breeding methodologies optimized to improve crops are often applied in the development of improved specialty types. However, because of the standards required for specialty traits, methods of development and improvement are usually more complex than those for common commodity crops. The same standards of performance are desired, but the genetics of the specialty traits often impose breeding criteria distinct from those of non-specialty possessing crops. Specifically, quality improvement programs have unique characteristics that require careful handling and monitoring during their development for specific needs. Adding value either via alternative products from the large volumes of grain produced or development of specialty types is of interest to producers and processors. This work assimilates the most topical results about quality improvement with contemporary plant breeding approaches.The objective of this book is to provide a summary of the germplasm, methods of development, and specific problems involved for quality breeding. In total, fourteen chapters, written by leading scientists involved in crop improvement research, provide comprehensive coverage of the major factors impacting specialty crop improvement.

Plant improvement has shifted its focus from yield, quality and disease resistance to factors that will enhance commercial export, such as early maturity, shelf life and better processing quality. Conventional plant breeding methods aiming at the improvement of a self-pollinating crop, such as wheat, usually take 10-12 years to develop and release of the new variety. During the past 10 years, significant advances have been made and accelerated methods have been developed for precision breeding and early release of crop varieties. This work summarizes concepts dealing with germplasm enhancement and development of improved varieties based on innovative methodologies that include doubled haploidy, marker assisted selection, marker assisted background selection, genetic mapping, genomic selection, high-throughput genotyping, high-throughput phenotyping, mutation breeding, reverse breeding, transgenic breeding, shuttle breeding, speed breeding, low cost high-throughput field phenotyping, etc. It is an important reference with special focus on accelerated development of improved crop varieties.

This book presents latest work in the field of plant biotechnology regarding high-efficiency micropropagation for commercial exploitation at low labor and equipment costs. The book consists of 18 chapters on establishing advanced culture systems, techniques as well as latest modification protocols on a variety of crops. It also discusses new methods such as nylon film culture system, light-emitting diode and wireless light-emitting diode system, stem elongation, wounding manipulation and shoot tip removal, in vitro hydroponic and microponic culture system, thin cell layer culture system etc. Plant cell tissue has been developed more than fifty years ago. Since then applications of in vitro plant propagation expanded rapidly all around the world and played as an important role in agricultural and horticultural systems. This book will be of interest to teachers, researchers, scientists, capacity builders and policymakers. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences.

This book addresses Synthetic Biology (SynBio), a new and promising biotechnology that has attracted much interest from both a scientific and a policy perspective. Yet, questions concerning the patentability of SynBio inventions have not been examined in detail so far; as a result, it remains unclear whether these inventions are patentable on the basis of current norms and case law. The book addresses this question, focusing especially on the subject matter's eligibility and moral criteria. It provides an overview of the legislation and decisions applicable to SynBio patents and examines this new technology in view of the ongoing debate over the patentability of biotechnologies in general. The legal analysis is complemented by the practical examination of several patent applications submitted to the European and US patent offices (EPO and USPTO), and by an assessment of the patent issues that are likely to be raised by future SynBio developments.

Global industrial growth has resulted in numerous pollutants being introduced into the environment. It has additionally caused decreased water availability for agricultural activity in developing countries, which, in turn, has compelled farmers to use wastewater irrigation. In advanced agricultural systems, farmers are adapting various strategies to achieve a higher yield and thus sustain crop productivity. Consequent to the introduction of contaminants in the environment, soil pollutants have become a critical issue. Selection of disease-resistant, high-yielding crop varieties, and extensive fertilizer applications are quite common among farming communities. This book provides insight into environmental pollutants with special reference to their interference with plant nutrition. It additionally discusses the physiological aspects of plant nutrition. This book enhances current knowledge of the effects of pollutants on plant growth and physiology.