Single molecule techniques, including single molecule
fluorescence, optical tweezers, and scanning probe microscopy,
allow for the manipulation and measurement of single biological
molecules within a live cell or in culture. These approaches,
amongst the most exciting tools available in biology today, offer
powerful new ways to elucidate biological function, both in terms
of revealing mechanisms of action on a molecular level as well as
tracking the behaviour of molecules in living cells. This book
provides the first complete and authoritative treatment of this
rapidly emerging field, explicitly from a biological
perspective.
The contents are organized by biological system or molecule.
Each chapter discusses insights that have been revealed about their
mechanism, structure or function by single molecule techniques.
Among the topics covered are enzymes, motor proteins, membrane
channels, DNA, ribozymes, cytoskeletal proteins, and other key
molecules of current interest. An introduction by the editor
provides a concise review of key principles and an historical
overview. The last section discusses applications in molecular
diagnostics and drug discovery.
* Organized by biological system or molecule.
* Each chapter discusses insights into mechanism of action,
structure, and function
* Covers enzymes, motor proteins, membrane channels, DNA,
ribozymes, etc.
* Includes an introduction to key principles and an historical
overview.
* Discusses applications in molecular diagnostics and drug
discovery.
* Provides an expert's perspective on future developments.
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