This Brief highlights different approaches used to create stable
cellulase and its use in different fields. Cellulase is an
industrial enzyme with a broad range of significant applications in
biofuel production and cellulosic waste management. Cellulase 7a
from Trichoderma reesei is the most efficient enzyme in the bio
hydrolysis of cellulose. In order to improve its thermal stability,
it can be engineered using a variety of approaches, such as
hydrophobic interactions, aromatic interactions, hydrogen bonds,
ion pairs and disulfide bridge creation.
General
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