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An Introduction To The Theory And Use Of the Microscope (Paperback)
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An Introduction To The Theory And Use Of the Microscope (Paperback)
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AN INTRODUCTION TO The Theory and Use of the Microscope BY C. R.
MARSHALL, M. A., M. D., LL. D. Professor of Materia Medico, and
Therapeutics in the University of Aberdeen. AND H. D. GRIFFITH, B.
A. Carnegie Teaching Fellow in Natural Philosophy in the University
of Aberdeen. WITH TWENTY-NINE FIGURES IN THE TEXT AND THREE PLATES
LONDON GEORGE ROUTLEDGE SONS, LTD. BROADWAY HOUSE 68-74 CARTER
LANE, E. G. PRINTED IN GREAT BRITUN BY W. JOLLY AND SONS, LTD.,
ABERDEEN. FOREWORD. THE inspiration of this brochure was the
institution of lectures and practical work on Microscopy as part of
the class of Medical Physics in the University of Aberdeen. It was
felt that a small textbook covering the work of the systematic
lectures would be helpful . to the student and might aid him to
realize the capabilities, limitations and proper method of use of
the instrument. A chapter on the elementary mathematical treat ment
of certain problems discussed in the text has been added. It is
hoped that the work will prove of value to all students who require
a microscope in their studies as well as to those amateur
microscopists who wish to understand the fundamental principles on
which Microscopy is based. C. R. M. H. D. G. January, 1928.
CONTENTS. PAGE. INTRODUCTION i 10 The Simple Convex Lens Formation
of the Image The Influence of the Eye Visibility of Objects The
Simple Microscope The Compound Microscope. THE LENSES OF THE
MICROSCOPE . . . n 32 Chromatic Aberration Spherical Aberration.
OBJECTIVES. Achromatic, Semi-apochromatic, Apochromatic Focal
Length Numerical Aperture Depth of Focus Working Distance Flatness
of Field. EYEPIECES. Huyghenian Positive-Ramsden Disc Magnifying
Power. CONDENSERS. AbbeAchromatic - Critical Illumination Focal
Length Dark Ground Condensers Ultra Microscopy. RESOLUTION 33 36
Limit of Microscopic Vision Ultra-Violet Microscopy Abbes
Diffraction Theory. THE STAND 37 44 The Foot The Stage The Substage
The Body The Nose Piece The Limb Choice of Stand Choice of
Objectives Choice of Eyepieces Testing Objectives. ILLUMINANTS 45
49 Illumination of Transparent Objects Dark Ground Effects Oblique
Illumination Illu ination of Opaque Objects, vili. CONTENTS
ADJUSTMENT 50 56 Adjustment of Mirror Focusing of Condenser
Centering of Condenser Adjustment of Iris Diaphragm Adjustment of
Tube Length Changing Objectives Changing Eyepieces Care of the
Microscope., MlCROMETRY 57 62 Stage and Eyepiece Micrometers
Photo-Micrography Measurement of Magnification Drawing Eyepieces
Measurement of Thick ness. SPECTROSCOPY . ... 6364 SPECIAL
MICROSCOPES ...... 65 72 The Metallurgical Microscope The Petro
logical Microscope. Binocular Microscopes Greenough Wenham,
Compound Prism Abbes Stereoscopic Eye piece Binocular Vision
Binocular Rivalry. PHYSICAL PROOFS 73 84 Magnification Numerical
Aperture Depth of Focus Ramsden Circle Chromatic Correction Sine
Condition and Aplanatism Resolution. INDEX, 85 90 PLATES to face
pp. 6, 28, 36 AN INTRODUCTION TO THE THEORY AND USE OF THE
MICROSCOPE. THE function of the microscope is to reveal detail of
the structure of objects too small to be visible to the unaided
eye. This end is attained by the use of a series of lenses which
make the object appear magnified to the observer. The magnification
to be of value must extend to the finest detail of the object and
each successive stage of magnification should reveal structure
invisible without its aid.So-called c resolution J of detail in an
object does not of necessity result from mere optical
magnification. Magnification is necessary to attain it, but other
factors are involved. It is the aim of this brochure to explain the
principles of microscopy and the manipulations by which resolution
is obtained. For these purposes it is essential to consider first
certain properties of the simple convex lens, and some of the
conditions governing the visibility of objects. THE SIMPLE CONVEX
LENS...
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