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Books > Science & Mathematics > Science: general issues > Scientific equipment & techniques, laboratory equipment > Microscopy
Details the use of advanced AFMs and addresses all types of functional AFMs First book to focus on application of AFM for energy research Enables readers to operate an AFM successfully and to understand the data obtained Covers new achievements in AFM instruments, including higher speed and resolution, automatic and deep learning AFM, and how AFM is being combined with other new methods like IR and Raman microscopy
Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualization describes the essential techniques that can be used for histochemical investigations in both light and transmission electron microscopy. The book begins by discussing techniques in light microscopy. It reviews classical methods of visualization, histochemical and histoenzymatic methods, and methods used to visualize cell proliferation and apoptosis. Next, the book examines the cytochemical methods used in electron microscopy with traditional techniques, as well as more specialized methods. The final section provides an overview of image analysis and describes how image processing methods can be used to extract vital information. A 16-page insert supplies color illustrations to enhance the text. Techniques will continue to adapt to the latest technological innovations, allowing more and more precise quantification of images. These developments are essential to the biological as well as the medical sciences. This manual is a critical resource for novice and experienced researchers, technicians, and students who need to visualize what happens in the cell, the molecules expressed, the main enzymatic activities, and the repercussions of the molecular activities upon the structure of the cells in the body.
Electron microscopy is now a mainstay characterization tool for solid state physicists and chemists as well as materials scientists. Containing the proceedings from the Electron Microscopy and Analysis Group (EMAG) conference in September 2003, this volume covers current developments in the field, primarily in the UK. These conferences are biennial events organized by the EMAG of the Institute of Physics to provide a forum for discussion of the latest developments in instrumentation, techniques, and applications of electron and scanning probe microscopies.
Comprises four parts, the first of which provides an overview of the topics that are developed from fundamental principles to more advanced levels in the other parts. Presents in the second part an in-depth introduction to the relevant background in molecular and cellular biology and in physical chemistry, which should be particularly useful for students without a formal background in these subjects. Provides in the third part a detailed treatment of microscopy techniques and optics, again starting from basic principles. Introduces in the fourth part modern statistical approaches to the determination of parameters of interest from microscopy data, in particular data generated by single molecule microscopy experiments. Uses two topics related to protein trafficking (transferrin trafficking and FcRn-mediated antibody trafficking) throughout the text to motivate and illustrate microscopy techniques
The past three decades have witnessed the great success of lithium-ion batteries, especially in the areas of 3C products, electrical vehicles, and smart grid applications. However, further optimization of the energy/power density, coulombic efficiency, cycle life, charge speed, and environmental adaptability are still needed. To address these issues, a thorough understanding of the reaction inside a battery or dynamic evolution of each component is required. Microscopy and Microanalysis for Lithium-Ion Batteries discusses advanced analytical techniques that offer the capability of resolving the structure and chemistry at an atomic resolution to further drive lithium-ion battery research and development. * Provides comprehensive techniques that probe the fundamentals of Li-ion batteries. * Covers the basic principles of the techniques involved as well as its application in battery research. * Describes details of experimental set-ups and procedure for successful experiments. This reference is aimed at researchers, engineers, and scientists studying lithium-ion batteries including chemical, materials, and electrical engineers, as well as chemists and physicists.
More than half of the world's petroleum is to be found in carbonate rocks, for example in the Middle East, the former USSR and in North America. These rocks show a bewildering diversity of grains and textures, due in part to the wealth of different fossil organisms which have contributed to carbonate sedimentation, and in part to a wide variety of diagenetic processes which can radically modify textures and obscure the depositional fabric. Careful petrographic study with a polarising microscope is a key element of any study of carbonate sediments, as a companion to field or core logging, and as a necessary precursor to geochemical analysis. This atlas, which illustrates in full colour a range of features not attempted in any general textbook, is designed as a laboratory manual to keep beside the microscope, as an aid to identifying grain types and textures in carbonates. It appeals alike to undergraduate and graduate students and to professionals in teaching institutions, research laboratories and industry.
This research-level reference provides a review of the morphological techniques that have become a primary method of anatomical study correlating structure and function in lung physiology and pathology. Detailing the evolution of anatomy as a research discipline, it explores general structural techn
This interdisciplinary book, Advanced Microscopy: A Strong Analytical Tool in Materials Science, covers the methodology and applications of different advanced microscopic techniques in various research fields, including chemistry, nanotechnology, polymers, chemical engineering, and biomedical engineering, providing an informative overview that helps to determine the best applications for advanced materials. Materials usually behave very differently at nanoscale in all aspects, and this volume shows how microscopy can help provide a detailed understanding of materials such as semiconductors, metals, polymers, biopolymers, etc. The volume illustrates advanced microscopic techniques that include scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM), confocal microscopy, and others. The microscopy techniques presented in the volume show applications in many areas of science, including botany and plant science, medicine, nanotechnology, chemistry, food science, waste management, and others. This book presents the diverse advanced microscopic techniques for researchers, giving a better understanding as well as implementation of novel techniques in materials science.
Includes recent research and development in the areas of omics and microbial bioremediation Covers the broad environmental pollution control approach such as metagenomics, metabolomics, fluxomics, bioremediation, and biodegradation of industrial wastes Reviews metagenomics and waste management, and recycling for environmental cleanup Describes the metagenomic methodologies and best-practices, from sample collection to data analysis for taxonomies Explores various microbial degradation pathways and detoxification mechanisms for organic and inorganic contaminants of wastewater with their gene expression
Work with individual atoms and molecules aims to demonstrate
that miniaturized electronic, optical, magnetic, and mechanical
devices can operate ultimately even at the level of a single atom
or molecule. As such, atomic and molecular manipulation has played
an emblematic role in the development of the field of nanoscience.
New methods based on the use of the scanning tunnelling microscope
(STM) have been developed to characterize and manipulate all the
degrees of freedom of individual atoms and molecules with an
unprecedented precision. In the meantime, new concepts have emerged
to design molecules and substrates having specific optical,
mechanical and electronic functions, thus opening the way to the
fabrication of real nano-machines. Manipulation of individual atoms
and molecules has also opened up completely new areas of research
and knowledge, raising fundamental questions of "Optics at the
atomic scale," "Mechanics at the atomic scale," Electronics at the
atomic scale," "Quantum physics at the atomic scale," and
"Chemistry at the atomic scale." This book aims to illustrate the
main aspects of this ongoing scientific adventure and to anticipate
the major challenges for the future in "Atomic and molecular
manipulation" from fundamental knowledge to the fabrication of
atomic-scale devices.
The detection and measurement of the dynamic interactions of
proteins within the living cell are critical to our understanding
of cell physiology and pathophysiology. With FRET microscopy and
spectroscopy techniques, basic and clinical scientists can make
such measurements at very high spatial and temporal resolution. But
sources of background information about these tools are very
limited, so this book fills an important gap. It covers both the
basic concepts and theory behind the various FRET microscopy and
spectroscopy techniques, and the practical aspects of using the
techniques and analyzing the results. The critical tricks for
obtaining a good FRET image and precisely quantitating the signals
from living specimens at the nanomolecular level are explained.
Valuable information about the preparation of biological samples
used for FRET image analysis is also provided.
Comprises four parts, the first of which provides an overview of the topics that are developed from fundamental principles to more advanced levels in the other parts. Presents in the second part an in-depth introduction to the relevant background in molecular and cellular biology and in physical chemistry, which should be particularly useful for students without a formal background in these subjects. Provides in the third part a detailed treatment of microscopy techniques and optics, again starting from basic principles. Introduces in the fourth part modern statistical approaches to the determination of parameters of interest from microscopy data, in particular data generated by single molecule microscopy experiments. Uses two topics related to protein trafficking (transferrin trafficking and FcRn-mediated antibody trafficking) throughout the text to motivate and illustrate microscopy techniques
Image Analysis of Food Microstructure offers a condensed guide to the most common procedures and techniques by which quantitative microstructural information about food can be obtained from images. The images are selected from a broad range of food items, including macroscopic images of meat and finished products such as pizza, and the microstructures of cheeses, dough and baked goods, ice cream, fruits and vegetables, emulsions, foams, and gels. The book informs food scientists about the image processing and measurement tools used to characterize a variety of microstructures in foods, using high-quality image techniques to illustrate chemical composition, thermo-mechanical processing, and genetic and structural properties. These different types of images used to measure various aspects of structure include: macroscopic light photography, confocal light microscopy, electron microscopy, atomic force microscope images, magnetic resonance, and computed tomography. Then the text explains how to interpret images to produce data, plot the results in different graphs, and identify trends. Examples using these image analysis techniques show typical results that researchers can expect and recreate. Image Analysis of Food Microstructure summarizes the basic procedures that can be useful in various aspects of food research, from nutraceuticals to cooking and food processing. It presents the processing of images and mathematical principles needed for image analyses in a step-by-step approach to extract key information from the images obtained.
Electron microscopy is now a mainstay characterization tool for solid state physicists and chemists as well as materials scientists. Containing the proceedings from the Electron Microscopy and Analysis Group (EMAG) conference in September 2003, this volume covers current developments in the field, primarily in the UK. These conferences are biennial events organized by the EMAG of the Institute of Physics to provide a forum for discussion of the latest developments in instrumentation, techniques, and applications of electron and scanning probe microscopies.
As the selection of material for particular engineering properties becomes increasingly important in keeping costs down, methods for evaluating material properties also become more relevant. One such method examines the geometry of grain boundaries, which reveals much about the properties of the material. Studying material properties from their geometrical measurements, The Measurement of Grain Boundary Geometry provides a framework for a specialized application of electron microscopy for metals and alloys and, by extension, for ceramics, minerals, and semiconductors. The book presents an overview of the developments in the theory of grain boundary geometry and its practical applications in material engineering. It also covers the tunneling electron microscope (TEM), experimental aspects of data collection, data processing, and examples from actual investigations. Each step of the analysis process is clearly described, from data collection through processing, analysis, representation, and display to applications. The book also includes a glossary of terms. Exploring both the experimental and analytical aspects of the subject, this practical reference guide is essential for researchers and students involved in material properties, whether in physics, materials science, metallurgy, or physical chemistry.
Cell Membrane Nanodomains: From Biochemistry to Nanoscopy describes recent advances in our understanding of membrane organization, with a particular focus on the cutting-edge imaging techniques that are making these new discoveries possible. With contributions from pioneers in the field, the book explores areas where the application of these novel techniques reveals new concepts in biology. It assembles a collection of works where the integration of membrane biology and microscopy emphasizes the interdisciplinary nature of this exciting field. Beginning with a broad description of membrane organization, including seminal work on lipid partitioning in model systems and the roles of proteins in membrane organization, the book examines how lipids and membrane compartmentalization can regulate protein function and signal transduction. It then focuses on recent advances in imaging techniques and tools that foster further advances in our understanding of signaling nanoplatforms. The coverage includes several diffraction-limited imaging techniques that allow for measurements of protein distribution/clustering and membrane curvature in living cells, new fluorescent proteins, novel Laurdan analyses, and the toolbox of labeling possibilities with organic dyes. Since superresolution optical techniques have been crucial to advancing our understanding of cellular structure and protein behavior, the book concludes with a discussion of technologies that are enabling the visualization of lipids, proteins, and other molecular components at unprecedented spatiotemporal resolution. It also explains the ins and outs of the rapidly developing high- or superresolution microscopy field, including new methods and data analysis tools that exclusively pertain to these techniques. This integration of membrane biology and advanced imaging techniques emphasizes the interdisciplinary nature of this exciting field. The array of contributions from leading world experts makes this book a valuable tool for the visualization of signaling nanoplatforms by means of cutting-edge optical microscopy tools.
Introduces both optical microscopy and medical imaging with an emphasis on recurring themes such as resolution and contrast to reinforce understanding. Includes many illustrations and boxed material that give more detailed explanations. Features hands-on activities and experiments. Provides end-of-chapter problems for self-study. Offers supplementary online materials including a solutions manual.
Because of its simplicity of use and quantitative results, Scanning Electrochemical Microscopy (SECM) has become an indispensable tool for the study of surface reactivity. The fast expansion of the SECM field over several years has been fueled by the introduction of new probes, commercially available instrumentation, and new practical applications. Scanning Electrochemical Microscopy, Third Edition offers essential background and in-depth overviews of specific applications in self-contained chapters. The vitality and growing popularity of SECM over the past 30+ years have largely been determined by its versatility and capability to remain useful in the changing scientific and technological environments. New applications reported during the last decade reflect significant current activity in biomedical and energy-related research. This thoroughly updated edition provides up-to-date comprehensive reviews of different aspects of SECM. New chapters by renowned professionals in the field cover recent advances in different areas of SECM including nanoSECM, surface reactions and films, batteries, and fuel cells. Expanded coverage of electrocatalysis and surface interrogation as well as photoelectrochemistry and photoelectrocatalysis are also provided. Useful for a broad range of interdisciplinary research-from biological systems to nanopatterning-this book is invaluable to all interested in learning and applying SECM.
Biomedical photonics is currently one of the fastest growing fields, connecting research in physics, optics, and electrical engineering coupled with medical and biological applications. It allows for the structural and functional analysis of tissues and cells with resolution and contrast unattainable by any other methods. However, the major challenges of many biophotonics techniques are associated with the need to enhance imaging resolution even further to the sub-cellular level as well as translate them for in vivo studies. The tissue optical clearing method uses immersion of tissues into optical clearing agents (OCAs) that reduces the scattering of tissue and makes tissue more transparent and this method has been successfully used ever since. This book is a self-contained introduction to tissue optical clearing, including the basic principles and in vitro biological applications, from in vitro to in vivo tissue optical clearing methods, and combination of tissue optical clearing and various optical imaging for diagnosis. The chapters cover a wide range of issues related to the field of tissue optical clearing: mechanisms of tissue optical clearing in vitro and in vivo; traditional and innovative optical clearing agents; recent achievements in optical clearing of different tissues (including pathological tissues) and blood for optical imaging diagnosis and therapy. This book provides a comprehensive account of the latest research and possibilities of utilising optical clearing as an instrument for improving the diagnostic effectiveness of modern optical diagnostic methods. The book is addressed to biophysicist researchers, graduate students and postdocs of biomedical specialties, as well as biomedical engineers and physicians interested in the development and application of optical methods in medicine. Key features: The first collective reference to collate all known knowledge on this topic Edited by experts in the field with chapter contributions from subject area specialists Brings together the two main approaches in immersion optical clearing into one cohesive book
This new volume, Microscopy Applied to Materials Sciences and Life Sciences. focuses on recent theoretical and practical advances in polymers and their blends, composites, and nanocomposites related to their microscopic characterization. It highlights recent accomplishments and trends in the field of polymer nanocomposites and filled polymers related to microstructural characterization. This book gives an insight and better understanding into the development in microscopy as a tool for characterization. The book emphasizes recent research work in the field of microscopy in life sciences and materials sciences mainly related to its synthesis, characterizations, and applications. The book explains the application of microscopic techniques in life sciences and materials sciences, and their applications and state of current research carried out. The book aims to foster a better understanding of the properties of polymer composites by describing new techniques to measure microstructure property relationships and by utilizing techniques and expertise developed in the conventional filled polymer composites. Characterization techniques, particularly microstructural characterization, have proven to be extremely difficult because of the range of length-scales associated with these materials. Topics include: *Instrumentation and Techniques: advances in scanning probe microscopy, SEM, TEM, OM. 3D imaging and tomography, electron diffraction techniques and analytical microscopy, advances in sample preparation techniques in-situ microscopy, correlative microscopy in life and material sciences, low voltage electron microscopy. *Life Sciences: Structure and imaging of biomolecules, live cell imaging, neurobiology, organelles and cellular dynamics, multi-disciplinary approaches for medical and biological sciences, microscopic application in plants, microorganism and environmental science, super resolution microscopy in biological sciences. *Materials Sciences: materials for nanotechnology, metals alloys and inter-metallic, ceramics, composites, minerals and microscopy in cultural heritage, thin films, coatings, surfaces and interfaces, carbon based materials, polymers and soft materials and self-assembled materials, semiconductors and magnetic materials. Polymers and inorganic nanoparticles. The volume will be of significant interest to scientists working on the basic issues surrounding polymers, nanocomposites, and nanoparticulate-filled polymers, as well as those working in industry on applied problems, such as processing. Because of the multidisciplinary nature of this research, the book will be valuable to chemists, materials scientists, physicists, chemical engineers, and processing specialists who are involved and interested in the future frontiers of blends.
In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization of genes: ° hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method) ° hybridization prior to embedding (pre-embedding) ° hybridization on ultrathin sections of frozen tissue (frozen tissue method). For each technique, the different stages are described in detail: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products. The book combines theory and practice, starting with the basic principles, then breaking down the experimental process into successive steps illustrated by numerous diagrams, detailed protocols, and tables. This is all done in a format that uses parallel columns to convey useful comments next to the theory and practical details alongside each stage of the protocol. Additionally, the summary tables provide the criteria for choosing the probe type and technique, and a detailed index aids in the search for information. in situ Hybridization In Electron Microscopy is an essential companion for applying these methods at the electron microscopic level.
Detecting Signals at the Single Molecule Level: Pioneering Achievements in Microscopy Recent advances have led to such remarkable improvements in fluorescence lifetime imaging microscopy's (FLIM) capacity for contrast and sensitivity that researchers can now employ it to detect signals at the single molecule level. FLIM also offers the additional benefit of independence from fluorophore concentration and excitation intensity. Moreover, its unique sensitivity makes it an excellent reporter of conformational changes and of variations in the molecular surroundings of biological molecules. Most of this improvement and discovery have occurred during the past decade, and, to date, information that would benefit a broad range of researchers remains scattered in the literature. Edited by two of the top pioneers in the field, FLIM Microscopy in Biology and Medicine presents the fundamentals of FLIM along with a number of advanced considerations so that a wider audience can appreciate recent and potential improvements that make it such a valuable tool. New Opportunities for Biomedical Researchers... New Challenges for Microscopy Researchers Discussion sections in all the chapters clearly show the challenges for implementing FLIM for various applications. Certain chapters discuss limits on the number of photons required for highly accurate lifetime determinations, as well as the accuracy with which multiple, closely associated lifetime components can reliably be determined. Such considerations are important for the user when he or she is selecting the most advantageous method of FLIM to use for a particular application. While this book provides an introduction for those new to FLIM, it gathers a wealth of material to enhance the work of experts involved in pioneering technological improvements, as well as those research opportunities in this unique and promising area of microscopy.
This dissertation examines the cultural and educational history of central Missouri between 1820 and 1860, and in particular, the issue of master-slave relationships and how they affected education (broadly defined as the transmission of Southern culture). Although Missouri had one of the lowest slave populations during the Antebellum period, Central Missouri - or what became known as Little Dixie - had slave percentages that rivaled many regions and counties of the Deep South. However, slaves and slave owners interacted on a regular basis, which affected cultural transmission in the areas of religion, work, and community. Generally, slave owners in Little Dixie showed a pattern of paternalism in all these areas, but the slaves did not always accept their masters' paternalism, and attempted to forge a life of their own.
Fluctuations in the level and pattern of international trade have a profound effect on the economies of less developed countries. This book explores the relationship between international trade and globl development through a series of essays which relate advances in trade theory to key issues in trade policy. The book, first published in 1991, is in honour of Jagdish Bhagwati and reflects the range and significance of his contributions to international economics.
This comprehensive reference work details the latest developments in fluorescence imaging and related biological quantification. It explores the most recent techniques in this imaging technology through the utilization and incorporation of quantification analysis which makes this book unique. It also covers super resolution microscopy with the introduction of 3D imaging and high resolution fluorescence. Many of the chapter authors are world class experts in this medical imaging technology. |
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