"Fundamentals of Light Microscopy and Electronic Imaging, Second
Edition" provides a coherent introduction to the principles and
applications of the integrated optical microscope system, covering
both theoretical and practical considerations. It expands and
updates discussions of multi-spectral imaging, intensified digital
cameras, signal colocalization, and uses of objectives, and offers
guidance in the selection of microscopes and electronic cameras, as
well as appropriate auxiliary optical systems and fluorescent
tags.The book is divided into three sections covering optical
principles in diffraction and image formation, basic modes of light
microscopy, and components of modern electronic imaging systems and
image processing operations. Each chapter introduces relevant
theory, followed by descriptions of instrument alignment and image
interpretation. This revision includes new chapters on live cell
imaging, measurement of protein dynamics, deconvolution microscopy,
and interference microscopy.
PowerPoint slides of the figures as well as other supplementary
materials for instructors are available at a companion website:
www.wiley.com/go/murphy/lightmicroscopy
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