In "Cytotoxic T-Cells: Methods and Protocols," leading experts in
the field provide a collection of state-of-the art methods and
protocols encompassing a wide array of systems biology approaches
for Cytotoxic T-Cell research. Dived into three main sections, the
first part of the volume analyzes the isolation of T Cells along
with their expansion and characterization according to different
methods. The second part describes required techniques for
intracellular signaling, monitoring of antigen T cell specific
responses, CTL exosomes, and microscopy and in vivo imaging applied
to CTL studies. The final section focuses on specialist
applications of molecular methods into the study of CTL, including
next generation sequencing of the Jack/stat pathway and CTL
involvement in bone remodeling and transplantation. Written in the
highly successful "Methods in Molecular Biology" series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and key tips on troubleshooting
and avoiding known pitfalls.
Authoritative and practical, "Cytotoxic T-Cells: Methods and
Protocols" seeks to aid scientists in the further study into
concepts of laboratory methods using systems biology. "
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