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Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Genetics (non-medical)
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Gene Targeting Protocols (Hardcover, 2000 ed.)
Loot Price: R4,501
Discovery Miles 45 010
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Gene Targeting Protocols (Hardcover, 2000 ed.)
Series: Methods in Molecular Biology, 133
Expected to ship within 10 - 15 working days
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The potential now exists in many experimental systems to transfer a
cloned, modified gene back into the genome of the host organism. In
the ideal situation, the cloned gene is returned to its homologous
location in the genome and becomes inserted at the target locus.
This process is a controlled means for the repair of DNA damage and
ensures accurate chromosome disjunction during meiosis. The
paradigm for thinking about the mechanism of this p- cess has
emerged primarily from two sources: (1) The principles of reaction
mechanics have come from detailed biochemical analyses of the RecA
protein purified from Escherichia coli; and (2) the principles of
information transfer have been derived from genetic studies carried
out in bacteriophage and fungi. A compelling picture of the process
of homologous pairing and DNA strand exchange has been influential
in directing investigators interested in gene t- geting
experiments. The ability to find and pair homologous DNA molecules
enables ac- rate gene targeting and is the central phenomenon
underlying genetic recombi- tion. Biochemically, the overall
process can be thought of as a series of steps in a reaction
pathway whereby DNA molecules are brought into homologous register,
the four-stranded Holliday structure intermediate is formed, hete-
duplex DNA is extended, and DNA strands are exchanged. Not much is
known about the biochemical pathway leading to homologous
recombination in euka- otes.
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