Books > Professional & Technical > Biochemical engineering
|
Buy Now
Gene Quantification (Paperback, Softcover reprint of the original 1st ed. 1998)
Loot Price: R5,455
Discovery Miles 54 550
|
|
Gene Quantification (Paperback, Softcover reprint of the original 1st ed. 1998)
Series: Advanced Biomedical Technologies
Expected to ship within 10 - 15 working days
|
Geneticists and molecular biologists have been interested in
quantifying genes and their products for many years and for various
reasons (Bishop, 1974). Early molecular methods were based on
molecular hybridization, and were devised shortly after Marmur and
Doty (1961) first showed that denaturation of the double helix
could be reversed - that the process of molecular reassociation was
exquisitely sequence dependent. Gillespie and Spiegelman (1965)
developed a way of using the method to titrate the number of copies
of a probe within a target sequence in which the target sequence
was fixed to a membrane support prior to hybridization with the
probe - typically a RNA. Thus, this was a precursor to many of the
methods still in use, and indeed under development, today. Early
examples of the application of these methods included the
measurement of the copy numbers in gene families such as the
ribosomal genes and the immunoglo bulin family. Amplification of
genes in tumors and in response to drug treatment was discovered by
this method. In the same period, methods were invented for
estimating gene num bers based on the kinetics of the reassociation
process - the so-called Cot analysis. This method, which exploits
the dependence of the rate of reassociation on the concentration of
the two strands, revealed the presence of repeated sequences in the
DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation
to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure
the abundance of RNAs in a mixed population."
General
Is the information for this product incomplete, wrong or inappropriate?
Let us know about it.
Does this product have an incorrect or missing image?
Send us a new image.
Is this product missing categories?
Add more categories.
Review This Product
No reviews yet - be the first to create one!
|
|
Email address subscribed successfully.
A activation email has been sent to you.
Please click the link in that email to activate your subscription.