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Bacterial, Phage and Molecular Genetics - An Experimental Course (Paperback, Softcover reprint of the original 1st ed. 1976)
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Bacterial, Phage and Molecular Genetics - An Experimental Course (Paperback, Softcover reprint of the original 1st ed. 1976)
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During the mid-forties bacteria and phages were dis- covered to be
suitable objects for the study of genet- ics. Genetic phenomena
such as mutation and recombina- tion, which had already been known
in eukaryotes for a long time, were now shown to exist in bacteria
and phages as well. New phenomena as lysogeny and trans- duction
were discovered, which gained great importance beyond the field of
microbial genetics. Bacteria and phages are of small size, multiply
rapid- ly, and have chemically defined growth requirements. Many
selective procedures can be applied to screen for rarely occurring
mutants or recombinants. Therefore, they offered ideal conditions
to investigate genetic processes and to interpret them in molecular
terms. Many new methods were developed (e. g. CsCl density gradient
centrifugation) and old techniques were im- proved and modified for
new purposes (e. g. chemical mutagenesis). Hypotheses, such as the
semiconservative replication of DNA, mutation by transition and
trans- version and operon regulation, have had an extraordi- narily
stimulating effect on the research in general genetics. Thus, in
the past two decades, from the ge- netics of microbes (including
fungi) the field of mo- lecular genetics developed. Many text books
compete in presenting the latest knowledge on this subject. But to
date there are only a few laboratory manuals which introduce the
student of biology to the manifold ex- perimental techniques of
microbial and molecular gene- tics. This laboratory manual is an
attempt to redress the balance.
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