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Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > DNA
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Environmental Applications of Nucleic Acid Amplification Technology (Hardcover)
Loot Price: R2,524
Discovery Miles 25 240
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Environmental Applications of Nucleic Acid Amplification Technology (Hardcover)
Expected to ship within 12 - 19 working days
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From the Preface
Antibody techniques have allowed us to study microorganisms in
situ. However, until recently all methodology lacked the
sensitivity necessary for environmental work where microorganisms
are in most cases present at very low concentrations or where
microbial ecosystems contain a myriad of different organisms. Gene
probes have been used successfully for a variety of samples, but
this method was still not sensitive enough. The next logical step
was the application of the recently developed DNA amplification
technique known as the polymerase chain reaction, or PCR. Since
then, many laboratories around the world have adopted PCR for
environmental work.
Samples obtained from soils, water and air are enormously complex
because they are unknown mixtures of DNA and other compounds. Thus,
procedures for target DNA amplification from the environment
require special attention. The PCR has allowed us to go beyond the
need for culturing prior to analysis of microbial communities. It
has been shown that even microorganisms that can be routinely grown
in the laboratory undergo some physiological changes when exposed
to the environment. One of these changes (first observed by R.
Colwell and colleagues) is known as the viable-but-non-culturable
state, and seems to be a common occurrence. Thus, the use of
culture techniques paint only part of the picture in terms of
microbial behavior under environmental conditions. The ability to
amplify nucleic acids by the PCR has brought about a myriad of very
ingenious modifications to the technique that can then be used to
study complex ecosystems. The manner in which the PCR can be
modified is only limited by the need and/or the imagination of the
researcher.
The first manual dedicated specifically to the analysis (by PCR) of
environmental samples, Environmental Applications of Nucleic Acid
Amplification Techniques presents state of the art methodology for
the detection of microorganisms in soil, water, air samples, as
well as the amplification of nucleic acids from fossil samples. The
manual gives step-by-step procedures for the analysis of these
samples. Although several publications have addressed the use of
Polymerase Chain Reaction technique, very few of them have been
directed toward the application of this technique to environmental
samples. This book fills this gap in the literature.
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