Great disparities exist between organisms with regard to the
relative ease of chromosomal mutagenesis and manipulation. In
Chromosomal Mutagenesis, a team of experts provide a variety of
chromosomal manipulation techniques, including insertional gene
disruptions, gene knockouts, stimulated homologous recombination
techniques and other novel tools, for both prokaryotic and
eukaryotic organisms, and attempt to expand the genetic toolbox
beyond model organisms. Following the format of the highly
successful Methods in Molecular Biology format, each chapter offers
step-by-step laboratory instructions, lists of the necessary
equipment and reagents, and tips on troubleshooting and avoiding
known pitfalls.
Comprehensive and cutting-edge, Chromosomal Mutagenesis covers
state-of-the-art techniques that are staged to expand, if not
revolutionize, genetic analysis in the long neglected and relevant
cell types.
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