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Bacterial Transcriptional Control - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2015)
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Bacterial Transcriptional Control - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2015)
Series: Methods in Molecular Biology, 1276
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This volume is designed to be a resource of proven techniques and
approaches for probing the activities of bacterial, eukaryotic, and
archaeal RNA polymerases. This book features a collection of in
vitro and in vivo technologies that will permit researchers to
purify and probe the position and stability of RNA polymerase
complexes at different points of the transcription cycle, analyze
the various translocations and intermolecular movements associated
with catalysis, define recruitment strategies, probe the roles of
transcription factors in each stage of the cycle, highlight
conserved and disparate fidelity mechanisms, analyze the resultant
transcripts, and study coordination of the nascent mRNA synthesis
by the RNA polymerase and mRNA translation by the ribosome. Written
in the highly successful Methods of Molecular Biology series
format, chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and key tips on troubles
troubleshooting and avoiding known pitfalls. Practical and timely,
Bacterial Transcriptional Controls: Methods and Protocols
highlights the breadth and depth of techniques that are likely to
continue shaping the transcription community in the future.
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